[Determination of content of iridoids and flavonoids in Tibetan medicine "Lanhua Longdan" by quantitative analysis of multi-components by single marker (QAMS)].

In this study, the content of iridoids and flavonoids in Tibetan medicine "Lanhua Longdan" was determined simul-taneously by quantitative analysis of multi-components by single marker(QAMS), which was used to verify the feasibility and applicability of the method in the application of Lanhua Longdan quality evaluation. Using HPLC with two typical elements gentiopicroside and isoorientin as the internal reference, the relative correction factor(RCF) between the mand loganin acid, swertiamarin, sweroside, isoscoparin-2″-ß-D-glucopyranoside and isoscoparin was determined and then used, to calculate the content of several other components to achieve QAMS. At the same time, the external standard method(ESM) was used to determine the contents of these 7 components in the medicinal materials, and the differences were compared to verify the accuracy and feasibility of QAMS. The results showed that the RCF repeatability is good. There were no significant differences in the determination results of the contents of 12 batches of 4 varieties of Tibetan medicine Lanhua Longdan obtained by QAMS and ESM. Therefore, the QAMS can be used for the quality evaluation of Lanhua Longdan, and provides a reference for the quality evaluation of multi-index components of Lanhua Longdan. The results showed that the content of iridoid and flavonoid in the dried product should not be less than 0.6% and 0.8% respectively.

Study on Chemotype of Tibetan Medicine"Bangjian"Multi-Origin Species Based on HPLC Fingerprint and UPLC-Q-TOF-MS / 世界科学技术-中医药现代化

Objective The HPLC fingerprints of different varieties of Tibetan medicine"Bangjian"were investigated to compare and analyse the differences in the chemical composition of different varieties and to further classify them,so as to provide reference for their quality control and safe clinical use.Methods HPLC method was used to establish the fingerprints of the different species of"Bangjian",and UPLC-Q-TOF-MS method was used to analyse the similarities and differences of the chemical components of the mainstream species and to identify the characteristic peaks.Chemometrics methods were used for the analysis including cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Results A total of 11 chromatographic peaks were identified from 93 batches of"Bangjian"samples.The HCA and PCA methods were used to classify the 93 batches into 2 categories,and then OPLS-DA was used to classify them into 3 categories in more detail,while 4 main components were selected according to the principle of VIP>1.The results of UPLC-Q-TOF-MS and chemometric showed that the Tibetan medicine"Bangjian"could be divided into iridoids with benzoyl fragments represented by Gentiana szechenyii Kanitz and monocyclic iridoids represented by Gentiana veitchiorum Hemsl.according to their chemotypes.The latter could be divided into alpine gentian group subtype and multi-branch group ornate subtype according to the content of components.The results of the chemotypic classification proved that the traditional classification of the"Bangjian"has a material basis in science but were also flawed.Conclusion The present study demonstrates that the established HPLC fingerprint can be used to classify the"Bangjian"of the complex base elements effectively,which is expected to provide an effective reference for the improvement of quality standards of"Bangjian"and clinical safety medication.