Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
1.
PLoS Med ; 13(8): e1002055, 2016 08.
Artículo en Inglés | MEDLINE | ID: mdl-27483136

RESUMEN

BACKGROUND: Antimicrobial resistance is a major issue in the Shigellae, particularly as a specific multidrug-resistant (MDR) lineage of Shigella sonnei (lineage III) is becoming globally dominant. Ciprofloxacin is a recommended treatment for Shigella infections. However, ciprofloxacin-resistant S. sonnei are being increasingly isolated in Asia and sporadically reported on other continents. We hypothesized that Asia is a primary hub for the recent international spread of ciprofloxacin-resistant S. sonnei. METHODS AND FINDINGS: We performed whole-genome sequencing on a collection of 60 contemporaneous ciprofloxacin-resistant S. sonnei isolated in four countries within Asia (Vietnam, n = 11; Bhutan, n = 12; Thailand, n = 1; Cambodia, n = 1) and two outside of Asia (Australia, n = 19; Ireland, n = 16). We reconstructed the recent evolutionary history of these organisms and combined these data with their geographical location of isolation. Placing these sequences into a global phylogeny, we found that all ciprofloxacin-resistant S. sonnei formed a single clade within a Central Asian expansion of lineage III. Furthermore, our data show that resistance to ciprofloxacin within S. sonnei may be globally attributed to a single clonal emergence event, encompassing sequential gyrA-S83L, parC-S80I, and gyrA-D87G mutations. Geographical data predict that South Asia is the likely primary source of these organisms, which are being regularly exported across Asia and intercontinentally into Australia, the United States and Europe. Our analysis was limited by the number of S. sonnei sequences available from diverse geographical areas and time periods, and we cannot discount the potential existence of other unsampled reservoir populations of antimicrobial-resistant S. sonnei. CONCLUSIONS: This study suggests that a single clone, which is widespread in South Asia, is likely driving the current intercontinental surge of ciprofloxacin-resistant S. sonnei and is capable of establishing endemic transmission in new locations. Despite being limited in geographical scope, our work has major implications for understanding the international transfer of antimicrobial-resistant pathogens, with S. sonnei acting as a tractable model for studying how antimicrobial-resistant Gram-negative bacteria spread globally.


Asunto(s)
Antibacterianos/uso terapéutico , Ciprofloxacina/uso terapéutico , Disentería Bacilar/tratamiento farmacológico , Shigella sonnei/efectos de los fármacos , Australia/epidemiología , Bután/epidemiología , Cambodia/epidemiología , Preescolar , Estudios Transversales , Farmacorresistencia Bacteriana/genética , Farmacorresistencia Bacteriana Múltiple/genética , Disentería Bacilar/epidemiología , Disentería Bacilar/microbiología , Genoma Bacteriano/genética , Humanos , Irlanda/epidemiología , Filogenia , Shigella sonnei/genética , Tailandia/epidemiología , Vietnam/epidemiología
2.
Arch Virol ; 160(4): 1021-33, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25663216

RESUMEN

Based on morphology and comparative nucleotide and protein sequence analysis, a new subfamily of the family Siphoviridae is proposed, named "Jerseyvirinae" and consisting of three genera, "Jerseylikevirus", "Sp3unalikevirus" and "K1glikevirus". To date, this subfamily consists of 18 phages for which the genomes have been sequenced. Salmonella phages Jersey, vB_SenS_AG11, vB_SenS-Ent1, vB_SenS-Ent2, vB_SenS-Ent3, FSL SP-101, SETP3, SETP7, SETP13, SE2, SS3e and wksl3 form the proposed genus "Jerseylikevirus". The proposed genus "K1glikevirus" consists of Escherichia phages K1G, K1H, K1ind1, K1ind2 and K1ind3. The proposed genus "Sp3unalikevirus" contains one member so far. Jersey-like phages appear to be widely distributed, as the above phages were isolated in the UK, Canada, the USA and South Korea between 1970 and the present day. The distinguishing features of this subfamily include a distinct siphovirus morphotype, genomes of 40.7-43.6 kb (49.6-51.4 mol % G+C), a syntenic genome organisation, and a high degree of nucleotide sequence identity and shared proteins. All known members of the proposed subfamily are strictly lytic.


Asunto(s)
Bacteriófagos/clasificación , Siphoviridae/clasificación , Bacteriófagos/química , Bacteriófagos/genética , Bacteriófagos/aislamiento & purificación , Escherichia coli/virología , Genoma Viral , Datos de Secuencia Molecular , Filogenia , República de Corea , Salmonella/virología , Análisis de Secuencia de ADN , Análisis de Secuencia de Proteína , Homología de Secuencia , Siphoviridae/química , Siphoviridae/genética , Siphoviridae/aislamiento & purificación
5.
BMC Microbiol ; 9: 155, 2009 Jul 31.
Artículo en Inglés | MEDLINE | ID: mdl-19646244

RESUMEN

BACKGROUND: With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000-2007 to identify likely incidents of laboratory cross contamination. METHODS: Serotyping and antimicrobial susceptibility testing was performed on all Salmonella isolates received in the NSRL. Phage typing was performed on all S. Typhimurium and S. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected S. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars. RESULTS: Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1). CONCLUSION: The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.


Asunto(s)
Tipificación de Bacteriófagos , Laboratorios , Salmonella/aislamiento & purificación , Electroforesis en Gel de Campo Pulsado , Contaminación de Equipos , Contaminación de Alimentos/análisis , Microbiología de Alimentos , Pruebas de Sensibilidad Microbiana , Salmonella/clasificación , Serotipificación
6.
Nat Commun ; 10(1): 4828, 2019 10 23.
Artículo en Inglés | MEDLINE | ID: mdl-31645551

RESUMEN

Shigella sonnei increasingly dominates the international epidemiological landscape of shigellosis. Treatment options for S. sonnei are dwindling due to resistance to several key antimicrobials, including the fluoroquinolones. Here we analyse nearly 400 S. sonnei whole genome sequences from both endemic and non-endemic regions to delineate the evolutionary history of the recently emergent fluoroquinolone-resistant S. sonnei. We reaffirm that extant resistant organisms belong to a single clonal expansion event. Our results indicate that sequential accumulation of defining mutations (gyrA-S83L, parC-S80I, and gyrA-D87G) led to the emergence of the fluoroquinolone-resistant S. sonnei population around 2007 in South Asia. This clone was then transmitted globally, resulting in establishments in Southeast Asia and Europe. Mutation analysis suggests that the clone became dominant through enhanced adaptation to oxidative stress. Experimental evolution reveals that under fluoroquinolone exposure in vitro, resistant S. sonnei develops further intolerance to the antimicrobial while the susceptible counterpart fails to attain complete resistance.


Asunto(s)
Farmacorresistencia Bacteriana/genética , Disentería Bacilar/microbiología , Fluoroquinolonas , Genoma Bacteriano/genética , Shigella sonnei/genética , Antibacterianos/uso terapéutico , Asia Sudoriental/epidemiología , Asia Occidental/epidemiología , Teorema de Bayes , Ciprofloxacina/uso terapéutico , Girasa de ADN/genética , Topoisomerasa de ADN IV/genética , Evolución Molecular Dirigida , Disentería Bacilar/tratamiento farmacológico , Disentería Bacilar/epidemiología , Europa (Continente)/epidemiología , Evolución Molecular , Humanos , Epidemiología Molecular , Mutación , Filogenia , Polimorfismo de Nucleótido Simple , Shigella sonnei/fisiología
7.
Methods Mol Biol ; 1301: 35-40, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25862046

RESUMEN

In early July 2008, a cluster of six Salmonella Agona was identified in the Republic of Ireland. A dispersed, common source outbreak was suspected. Later in July a further case was identified and the Health Protection Agency in the UK indicated that they had 32 cases of S. Agona since Feb 2008. This chapter discusses how pulsed field gel electrophoresis was used to help confirm an outbreak and to trace the source of the outbreak.


Asunto(s)
Electroforesis en Gel de Campo Pulsado/métodos , Microbiología de Alimentos , Salmonella enterica/genética , Brotes de Enfermedades , Humanos , Salmonella enterica/aislamiento & purificación , Salmonella enterica/patogenicidad , Serotipificación
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA