RESUMEN
Pneumocystis organisms are airborne-transmitted fungal parasites that infect the lungs of numerous mammalian species with strong host specificity. In this study, we investigated the genetic diversity and host specificity of Pneumocystis organisms infecting Southeast Asian murid rodents through PCR amplification of two mitochondrial genes and tested the co-phylogeny hypothesis among these fungi and their rodent hosts. Pneumocystis DNA was detected in 215 of 445 wild rodents belonging to 18 Southeast Asian murid species. Three of the Pneumocystis lineages retrieved in our phylogenetic trees correspond to known Pneumocystis species, but some of the remaining lineages may correspond to new undescribed species. Most of these Pneumocystis species infect several rodent species or genera and some sequence types are shared among several host species and genera. These results indicated a weaker host specificity of Pneumocystis species infecting rodents than previously thought. Our co-phylogenetic analyses revealed a complex evolutionary history among Pneumocystis and their rodent hosts. Even if a significant global signal of co-speciation has been detected, co-speciation alone is not sufficient to explain the observed co-phylogenetic pattern and several host switches are inferred. These findings conflict with the traditional view of a prolonged process of co-evolution and co-speciation of Pneumocystis and their hosts.
Asunto(s)
Evolución Molecular , Variación Genética , Muridae/microbiología , Pneumocystis/genética , Neumonía por Pneumocystis/microbiología , Animales , Animales Salvajes/microbiología , Asia Sudoriental/epidemiología , ADN de Hongos/aislamiento & purificación , Genes Mitocondriales , Especificidad del Huésped , Pulmón/microbiología , Filogenia , Neumonía por Pneumocystis/epidemiología , Análisis de Secuencia de ADNRESUMEN
BACKGROUND: Histoplasma capsulatum and Pneumocystis organisms cause host infections primarily affecting the lung tissue. H. capsulatum is endemic in the United States of America and Latin American countries. In special environments, H. capsulatum is commonly associated with bat and bird droppings. Pneumocystis-host specificity has been primarily studied in laboratory animals, and its ability to be harboured by wild animals remains as an important issue for understanding the spread of this pathogen in nature. Bats infected with H. capsulatum or Pneumocystis spp. have been found, with this mammal serving as a probable reservoir and disperser; however, the co-infection of bats with both of these microorganisms has never been explored. To evaluate the impact of H. capsulatum and Pneumocystis spp. infections in this flying mammal, 21 bat lungs from Argentina (AR), 13 from French Guyana (FG), and 88 from Mexico (MX) were screened using nested-PCR of the fragments, employing the Hcp100 locus for H. capsulatum and the mtLSUrRNA and mtSSUrRNA loci for Pneumocystis organisms. RESULTS: Of the 122 bats studied, 98 revealed H. capsulatum infections in which 55 of these bats exhibited this infection alone. In addition, 51 bats revealed Pneumocystis spp. infection of which eight bats exhibited a Pneumocystis infection alone. A total of 43 bats (eight from AR, one from FG, and 34 from MX) were found co-infected with both fungi, representing a co-infection rate of 35.2% (95% CI = 26.8-43.6%). CONCLUSION: The data highlights the H. capsulatum and Pneumocystis spp.co-infection in bat population's suggesting interplay with this wild host.
Asunto(s)
Quirópteros , Coinfección/veterinaria , Histoplasma/aislamiento & purificación , Histoplasmosis/veterinaria , Infecciones por Pneumocystis/veterinaria , Pneumocystis/aislamiento & purificación , Animales , Argentina , Guyana , México , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , ARN Ribosómico/genética , Análisis de Secuencia de ADNRESUMEN
The humoral and cellular responses against excretory/secretory proteins and soluble extracts of Giardia intestinalis were evaluated in the course of experimental G. intestinalis infection in BALB/c mice. Production of IgG1, IgG2a, IgA, and IgE antibodies against excreted/secreted proteins and soluble extract was detected after infection by G. intestinalis. Specific IgA antibody against E/S proteins and soluble extract form intestinal fluids in infected mice was detected by ELISA. The Western blotting identified proteins of 30, 58, 63, and 83 kDa for IgA and IgG, respectively. High proliferation rate in vitro of spleen cell and secretion of interleukin-4 (IL-4) at 21 days p.i. after stimulation with excreted/secreted proteins and low proliferative response in the presence of soluble extract in infected BALB/c mice was observed. High production of interferon gamma (IFN-γ) and interleukin-5 (IL-5) at the time of decreasing cyst output (14-21 days p.i.) in infected mice was recorded, suggesting the important role of these cytokines in the control of the infection. Interestingly, progressive and gradual increase of the interleukin-10 after stimulation with both preparations was recorded from 7 days until 28 days after infection, indicating the possible regulatory effect of these antigens on the immune response during Giardia infection. Therefore, the infection by Giardia duodenalis stimulates a mixed response Th1 and Th2, mainly stimulated by excretory/secretory antigens. The immunogenicity of these antigens may be a suitable for identification of the proteins related with the effective immune response in the course of infection by G. duodenalsis.
Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Antígenos de Protozoos/inmunología , Giardia lamblia/inmunología , Giardiasis/inmunología , Inmunoglobulina G/sangre , Balance Th1 - Th2 , Animales , Antígenos de Protozoos/metabolismo , Ensayo de Inmunoadsorción Enzimática , Femenino , Giardia lamblia/metabolismo , Giardiasis/sangre , Giardiasis/parasitología , Interacciones Huésped-Parásitos , Inmunoglobulina A/sangre , Inmunoglobulina G/clasificación , Interferón gamma/sangre , Interleucina-10/sangre , Interleucina-4/sangre , Interleucina-5/sangre , Ratones , Ratones Endogámicos BALB C , Bazo/inmunología , Bazo/parasitología , Factores de TiempoRESUMEN
BACKGROUND: Pneumocystis without obvious accompanying pathology is occasionally reported in autopsied infant lungs. Its prevalence and significance are unknown. Interestingly, this mild infection induces a strong activation of mucus secretion-related genes in young immunocompetent rodents that has not been explored in infants. Excess mucus is induced by multiple airway offenders through nonspecific pathways and would explain a cofactor role of Pneumocystis in respiratory disease. We undertook characterization of the prevalence of Pneumocystis and associated mucus in infant lungs. METHODS: Samples from 128 infants (mean age, 101 days) who died suddenly and unexpectedly in Santiago during 1999-2004 were examined for Pneumocystis using nested polymerase chain reaction (nPCR) amplification of the P. jirovecii mtLSU ribosomal RNA gene and immunofluorescence microscopy (IF). Pneumocystis-negative infants 28 days and older and their age-closest positives were studied for MUC5AC expression and Pneumocystis burden by Western blot and quantitative PCR, respectively. RESULTS: Pneumocystis DNA was detected by nPCR in 105 of the 128 infants (82.0%) and Pneumocystis organisms were visualized by IF in 99 (94.3%) of the DNA-positive infants. The infection was commonest at 3-4 months with 40 of 41 (97.6%) infants of that age testing positive. MUC5AC was significantly increased in Pneumocystis-positive tissue specimens (P = .013). Death was unexplained in 113 (88.3%) infants; Pneumocystis was detected in 95 (84.0%) of them vs 10 of 15 (66.7%) with explained death (P = .28). CONCLUSIONS: A highly focal Pneumocystis infection associated to increased mucus expression is almost universally present in the lungs of infants dying unexpectedly in the community regardless of autopsy diagnosis.
Asunto(s)
Pulmón/metabolismo , Moco/metabolismo , Pneumocystis/aislamiento & purificación , Neumonía por Pneumocystis/epidemiología , Muerte Súbita del Lactante/epidemiología , Autopsia , Recuento de Colonia Microbiana , ADN de Hongos/genética , Femenino , Humanos , Lactante , Recién Nacido , Pulmón/microbiología , Pulmón/patología , Masculino , Microscopía , Mucina 5AC/metabolismo , Técnicas de Amplificación de Ácido Nucleico , Pneumocystis/genética , Neumonía por Pneumocystis/diagnóstico , Neumonía por Pneumocystis/microbiología , Prevalencia , Sensibilidad y Especificidad , Muerte Súbita del Lactante/diagnósticoRESUMEN
Histoplasma capsulatum is a dimorphic fungus that is widely distributed in the tropical or subtropical areas of the world and infects several mammalian hosts, mainly bats. Infective propagules grow in bat and bird droppings. A specific molecular marker, a highly sensitive fragment of a co-activator protein-coding gene (Hcp100), was used to detect H. capsulatum in lung samples of wild and captive bats from France using a nested polymerase chain reaction. To determine whether bats in France are potential carriers of H. capsulatum, 83 bats were sampled from two regions in France. Sixty-one specimens belonging to the Pteropus rodricensis (n = 45) and Rousettus aegyptiacus (n = 16) species were collected from a zoologic park (La Palmyre, western France). Twenty-two specimens were recovered from the Natural History Museum (Bourges) including the species Plecotus austriacus (n = 1), Pipistrellus pipistrellus (n = 3), and Nyctalus noctula (n = 18). From the lung DNA samples of 83 dead bats, only one sample of an N. noctula bat from Bourges amplified the H. capsulatum Hcp100 marker. The amplified product was sequenced and revealed a high similarity to the G217B H. capsulatum reference strain sequence that was deposited in the GenBank database. This finding suggests that H. capsulatum is an environmental pathogen in France that may infect bats.
Asunto(s)
Quirópteros/fisiología , Histoplasma/aislamiento & purificación , Histoplasmosis/veterinaria , Enfermedades Pulmonares/microbiología , Animales , Secuencia de Bases , ADN de Hongos , Francia/epidemiología , Histoplasmosis/epidemiología , Enfermedades Pulmonares/epidemiología , Reacción en Cadena de la Polimerasa/veterinariaRESUMEN
Pneumocystis carriage was detected in 12.5% of 104 cystic fibrosis (CF) patients during a prospective multicenter French study, with a prevalence of genotype 85C/248C and geographic variations. It was significantly associated with the absence of Pseudomonas aeruginosa colonization and a greater forced expiratory volume in 1 s. Results are discussed considering the natural history of CF.
Asunto(s)
Portador Sano/epidemiología , Fibrosis Quística/complicaciones , Infecciones por Pneumocystis/epidemiología , Pneumocystis carinii/aislamiento & purificación , Adolescente , Adulto , Portador Sano/microbiología , Femenino , Francia/epidemiología , Genotipo , Humanos , Masculino , Tipificación Molecular , Técnicas de Tipificación Micológica , Infecciones por Pneumocystis/microbiología , Pneumocystis carinii/clasificación , Pneumocystis carinii/genética , Estudios Prospectivos , Adulto JovenRESUMEN
In the present work, we report the characterization of a Cryptosporidium parvum strain isolated from a patient who nearly drowned in the Deule River (Lille, France) after being discharged from the hospital where he had undergone allogeneic stem cell transplantation. After being rescued and readmitted to the hospital, he developed fulminant cryptosporidiosis. The strain isolated from the patient's stools was identified as C. parvum II2A15G2R1 (subtype linked to zoonotic exposure) and inoculated into SCID mice. In this host, this virulent C. parvum isolate induced not only severe infection but also invasive gastrointestinal and biliary adenocarcinoma. The observation of adenocarcinomas that progressed through all layers of the digestive tract to the subserosa and spread via blood vessels confirmed the invasive nature of the neoplastic process. These results indicate for the first time that a human-derived C. parvum isolate is able to induce digestive cancer. This study is of special interest considering the exposure of a large number of humans and animals to this waterborne protozoan, which is highly tumorigenic when inoculated in a rodent model.
Asunto(s)
Adenocarcinoma/parasitología , Colangiocarcinoma/parasitología , Criptosporidiosis/diagnóstico , Cryptosporidium parvum/aislamiento & purificación , Cryptosporidium parvum/patogenicidad , Neoplasias Intestinales/parasitología , Ahogamiento Inminente/complicaciones , Animales , Criptosporidiosis/patología , Modelos Animales de Enfermedad , Heces/parasitología , Francia , Humanos , Ratones , Ratones SCIDRESUMEN
Bats belong to a wide variety of species and occupy diversified habitats, from cities to the countryside. Their different diets (i.e., nectarivore, frugivore, insectivore, hematophage) lead Chiroptera to colonize a range of ecological niches. These flying mammals exert an undisputable impact on both ecosystems and circulation of pathogens that they harbor. Pneumocystis species are recognized as major opportunistic fungal pathogens which cause life-threatening pneumonia in severely immunocompromised or weakened mammals. Pneumocystis consists of a heterogeneous group of highly adapted host-specific fungal parasites that colonize a wide range of mammalian hosts. In the present study, 216 lungs of 19 bat species, sampled from diverse biotopes in the New and Old Worlds, were examined. Each bat species may be harboring a specific Pneumocystis species. We report 32.9% of Pneumocystis carriage in wild bats (41.9% in Microchiroptera). Ecological and behavioral factors (elevation, crowding, migration) seemed to influence the Pneumocystis carriage. This study suggests that Pneumocystis-host association may yield much information on Pneumocystis transmission, phylogeny, and biology in mammals. Moreover, the link between genetic variability of Pneumocystis isolated from populations of the same bat species and their geographic area could be exploited in terms of phylogeography.
Asunto(s)
Portador Sano/veterinaria , Variación Genética , Pulmón/microbiología , Pneumocystis/clasificación , Pneumocystis/genética , Neumonía por Pneumocystis/veterinaria , Animales , Portador Sano/microbiología , Quirópteros , Análisis por Conglomerados , ADN de Hongos/química , ADN de Hongos/genética , ADN Mitocondrial/química , ADN Mitocondrial/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Datos de Secuencia Molecular , Filogenia , Pneumocystis/aislamiento & purificación , Neumonía por Pneumocystis/microbiología , Análisis de Secuencia de ADNRESUMEN
Genotypes of two different loci of the Pneumocystis jirovecii mitochondrial gene were studied in specimens from a total of 75 Pneumocystis pneumonia patients in Spain, France and Cuba. A new genotype of the mitochondrial small subunit rRNA gene of P. jirovecii (160A/196T) was identified, which was revealed to be the most common in these three countries, especially in Cuba where its proportion reached 93.8%. Our data imply that the new genotype might be circulating worldwide and also suggests that the distribution of P. jirovecii genotypes could be narrower in islands such as Cuba.
Asunto(s)
ADN Mitocondrial/genética , Variación Genética , Técnicas de Tipificación Micológica , Pneumocystis carinii/clasificación , Pneumocystis carinii/genética , Neumonía por Pneumocystis/epidemiología , Neumonía por Pneumocystis/microbiología , Adolescente , Adulto , Anciano , Niño , Preescolar , Cuba/epidemiología , Femenino , Francia/epidemiología , Genes de ARNr , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Epidemiología Molecular , Tipificación Molecular , Pneumocystis carinii/aislamiento & purificación , España/epidemiología , Adulto JovenRESUMEN
Is Pneumocystis pneumonia (PcP) a transmissible fungal disease? Does nosocomial PcP occur? Is there Pneumocystis transmission in the community? These questions, which could not be tackled before the 2000s, may at present be approached using either noninvasive detection methods or experimental transmission models. Represented by a unique entity (P. carinii) for almost one century, the Pneumocystis genus was shown to contain several species, being P. jirovecii the sole species identified in humans hitherto. Molecular methods combined with cross infection experiments revealed strong host specificity that precludes Pneumocystis inter-species transmission. In contrast, respiratory transmission between mammals of a same species is usually highly active, even between immunocompetent hosts. Other transmission ways could also exist. New data show that human being is the unique P. jirovecii reservoir; it would constitute the sole infection source in both hospital and community.
Asunto(s)
Infecciones por Pneumocystis/transmisión , Neumonía por Pneumocystis/transmisión , Animales , Infección Hospitalaria/epidemiología , Infección Hospitalaria/microbiología , Infección Hospitalaria/transmisión , Humanos , Transmisión Vertical de Enfermedad Infecciosa/estadística & datos numéricos , Modelos Biológicos , Pneumocystis/clasificación , Infecciones por Pneumocystis/epidemiología , Infecciones por Pneumocystis/microbiología , Neumonía por Pneumocystis/epidemiología , Neumonía por Pneumocystis/microbiologíaRESUMEN
To better understand the diffusion of Pneumocystis in the environment, airborne shedding of Pneumocystis carinii in the surrounding air of experimentally infected rats was quantified by means of a real-time polymerase chain reaction assay, in parallel with the kinetics of P. carinii loads in their lungs. P. carinii DNA was detected in the air 1 week after infection and increased until 4-5 weeks after infection before stabilizing. A significant correlation was shown between lung burdens and the corresponding airborne levels, suggesting the possibility of estimating the fungal lung involvement through quantification of Pneumocystis in the exhaled air.
Asunto(s)
Microbiología del Aire , Pneumocystis carinii/aislamiento & purificación , Neumonía por Pneumocystis/microbiología , Animales , ADN de Hongos/genética , ADN de Hongos/aislamiento & purificación , Modelos Animales de Enfermedad , Pulmón/microbiología , Micología/métodos , Reacción en Cadena de la Polimerasa/métodos , Ratas , Factores de TiempoRESUMEN
OBJECTIVES: To determine the rate and identify risk factors of Pneumocystis jirovecii (P. jirovecii) colonization among patients with systemic autoimmune diseases. METHODS: We conducted an observational study in patients with systemic autoimmune diseases in an internal medicine department. Each week, five patients with systemic diseases were randomly selected for colonization screening. Patients complaining of recent respiratory symptoms were excluded. P. jirovecii PCR was performed on induced sputum samples. Univariate and multivariate logistic regression analyses of clinical and biological data were performed to determine predictors of Pneumocystis colonization. Pneumocystis pneumonia occurrence in P. jirovecii-positive PCR patients was recorded during a 1-year follow-up. RESULTS: P. jirovecii was detected in 11/67 (16%) subjects. Comparing the features in P. jirovecii-positive and P. jirovecii-negative PCR patients, only male gender was significantly associated with Pneumocystis colonization. In multivariate analysis with regard to gender, the higher prevalence of P. jirovecii colonization in men was largely explained by higher daily CSs [odds ratio (OR) = 1.6; 95% CI 1.1, 2.3] and lower total lymphocyte level (OR = 0.9; 95% CI 0.8, 0.99). No P. jirovecii-positive PCR patient developed Pneumocystis pneumonia during the 1-year follow-up, but corticosteroid amounts were significantly lower at the end of follow-up than on inclusion. CONCLUSION: This is the first study on P. jirovecii colonization in patients with systemic autoimmune diseases. We found a high prevalence of colonization and identified CS therapy and lymphocyte counts as risk factors for colonization. We recommend screening for P. jirovecii colonization in patients with systemic autoimmune diseases receiving immunosuppressant treatment. Further studies are needed to determine the role of subclinical colonization in disease transmission and the persistence of Pneumocystis colonization.
Asunto(s)
Enfermedades Autoinmunes/microbiología , Infecciones por Pneumocystis/microbiología , Pneumocystis carinii/aislamiento & purificación , Anciano , Enfermedades Autoinmunes/complicaciones , Femenino , Humanos , Recuento de Linfocitos , Masculino , Persona de Mediana Edad , Infecciones por Pneumocystis/complicaciones , Prevalencia , Medición de Riesgo , Factores de Riesgo , Esputo/microbiologíaRESUMEN
At the end of the 20th century the unique taxonomically enigmatic entity called Pneumocystis carinii was identified as a heterogeneous group of microscopic Fungi, constituted of multiple stenoxenic biological entities largely spread across ecosystems, closely adapted to, and coevolving in parallel with, mammal species. The discoveries and reasoning that led to the current conceptions about the taxonomy of Pneumocystis at the species level are examined here. The present review also focuses on the biological, morphological and phylogenetical features of Pneumocystis jirovecii, Pneumocystis oryctolagi, Pneumocystis murina, P. carinii and Pneumocystis wakefieldiae, the five Pneumocystis species described until now, mainly on the basis of the phylogenetic species concept. Interestingly, Pneumocystis organisms exhibit a successful adaptation enabling them to dwell and replicate in the lungs of both immunocompromised and healthy mammals, which can act as infection reservoirs. The role of healthy carriers in aerial disease transmission is nowadays recognized as a major contribution to Pneumocystis circulation, and Pneumocystis infection of nonimmunosuppressed hosts has emerged as a public health issue. More studies need to be undertaken both on the clinical consequences of the presence of Pneumocystis in healthy carriers and on the intricate Pneumocystis life cycle to better define its epidemiology, to adapt existing therapies to each clinical context and to discover new drug targets.
Asunto(s)
Filogenia , Infecciones por Pneumocystis/epidemiología , Infecciones por Pneumocystis/microbiología , Pneumocystis/clasificación , Animales , Humanos , Pulmón/microbiología , Mamíferos , Pneumocystis/citología , Pneumocystis/genética , Pneumocystis/patogenicidad , Infecciones por Pneumocystis/patologíaRESUMEN
Feces from 142 animals were collected on 15 farms in the region of Brittany, France. Each sample was directly collected from the rectum of the animal and identified with the ear tag number. Animals were sampled three times, at 5, 15 and 22 weeks of age. After DNA extraction from stool samples, nested PCR was performed to amplify partial 18S-rDNA and 60 kDa glycoprotein genes of Cryptosporidium. The parasite was detected on all farms. One hundred out of 142 calves (70.4%) were found to be parasitized by Cryptosporidium. Amplified fragments were sequenced for Cryptosporidium species identification and revealed the presence of C. parvum (43.8%), C. ryanae (28.5%), and C. bovis (27%). One animal was infected with Cryptosporidium ubiquitum. The prevalence of these species was related to the age of the animal. C. parvum caused 86.7% of Cryptosporidium infections in 5-week-old calves but only 1.7% in 15-week-old animals. The analysis of the results showed that animals could be infected successively by C. parvum, C. ryanae, and C. bovis for the study period. C. parvum gp60 genotyping identifies 6 IIa subtypes of which 74.5% were represented by IIaA15G2R1. This work confirms previous studies in other countries showing that zoonotic C. parvum is the dominant species seen in young calves.
Asunto(s)
Enfermedades de los Bovinos/parasitología , Criptosporidiosis/veterinaria , Cryptosporidium/genética , Animales , Bovinos , Enfermedades de los Bovinos/epidemiología , Criptosporidiosis/epidemiología , Criptosporidiosis/parasitología , Cryptosporidium/clasificación , Cryptosporidium/aislamiento & purificación , ADN Protozoario/genética , ADN Protozoario/metabolismo , Heces/parasitología , Francia/epidemiología , Glicoproteínas/genética , Glicoproteínas/metabolismo , Estudios Longitudinales , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , Proteínas Protozoarias/genética , Proteínas Protozoarias/metabolismo , ARN Ribosómico 18S/genética , ARN Ribosómico 18S/metabolismo , Análisis de Secuencia de ADN/veterinariaRESUMEN
Blastocystis sp. is the most common eukaryotic parasite in the intestinal tract of humans. Due to its potential impact in public health, we determined the Blastocystis sp. subtypes (STs) and their relative frequency in symptomatic patients living in or in the vicinity of two Italian cities (Rome and Sassari). A total of 34 Blastocystis sp. isolates corresponding to 26 single and 4 mixed infections were subtyped using partial small subunit ribosomal RNA gene sequencing. From this molecular approach, the ST distribution in the present Italian population was as follows: ST3 (47.1%), ST2 (20.6%), ST4 (17.7%), ST1 (8.8%), and ST7, and ST8 (2.9%). As in almost all countries worldwide, ST3 was the most common ST reinforcing the hypothesis of its human origin. Together with a previous preliminary report, a total of seven STs (with the addition of ST5) have been found in Italian symptomatic patients. The wide range of STs identified in the Italian population suggest that Blastocystis sp. infection is not associated with specific STs even if some STs (ST1-ST4) are predominant as reported in all other countries. Since most of the STs identified in Italian patients are zoonotic, our data raise crucial questions concerning the identification of animal reservoirs for Blastocystis sp. and the potential risks of transmission to humans.
Asunto(s)
Infecciones por Blastocystis/parasitología , Blastocystis/clasificación , Blastocystis/genética , Tipificación Molecular , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Animales , Niño , Análisis por Conglomerados , ADN Protozoario/química , ADN Protozoario/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Femenino , Genes de ARNr , Genotipo , Humanos , Italia , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Filogenia , ARN Protozoario/genética , ARN Ribosómico 18S/genética , Análisis de Secuencia de ADN , Adulto JovenRESUMEN
This work reports for the first time the presence of two Pneumocystis species in wild Rattus norvegicus specimens from Thailand. Pneumocystis DNA was detected in 57.7% (15/26) wild rats without apparent association with typical pneumocystosis. Pneumocystis carinii was found alone in five rats (19.2%), Pneumocystis wakefieldiae was detected alone in six rats (23.1%), and two rats were infected by both species (7.7%). In addition, a new P. wakefieldiae variant sequence has been identified in three wild R. norvegicus specimens caught in the same geographical area. The high frequency of Pneumocystis in wild rats documented in this study and the apparent scarcity of severe pneumocystosis were consistent with an efficient circulation of rat Pneumocystis species in ecosystems.
Asunto(s)
Infecciones por Pneumocystis/veterinaria , Pneumocystis/clasificación , Pneumocystis/aislamiento & purificación , Enfermedades de los Roedores/epidemiología , Enfermedades de los Roedores/microbiología , Animales , ADN de Hongos/química , ADN de Hongos/genética , ADN de Hongos/aislamiento & purificación , ADN Ribosómico/química , ADN Ribosómico/genética , Infecciones por Pneumocystis/epidemiología , Infecciones por Pneumocystis/microbiología , Prevalencia , Ratas , Análisis de Secuencia de ADN , Tailandia/epidemiologíaRESUMEN
Blastocystis sp. has been described as the most common intestinal parasite in humans and has an increased impact in public health. To improve our understanding of the molecular epidemiology of this human-emerging parasite, we determined the Blastocystis subtypes (STs) and their relative frequency in Egyptian patients living in or in the vicinity of Cairo and presenting gastrointestinal symptoms. We obtained a total of 20 stool samples identified as positive for Blastocystis by microscopic examination of smears. Genotyping using partial small subunit ribosomal RNA gene analysis identified a total of 21 Blastocystis isolates corresponding to 19 single infections and one mixed infection (ST1 and ST3). Three STs were identified: ST3 was the most common ST in the present Egyptian population (61.90%) followed by ST1 (19.05%) and ST2 (19.05%). Together with previous studies carried out in different areas in Egypt, a total of five STs (ST1, ST2, ST3, ST4, and ST6) have been found in symptomatic patients. These data were compared to those available in the literature, and we underlined variations observed in the number and relative proportions of STs between and within countries. On the whole, it seemed that Blastocystis infection is likely not associated with specific STs even if some STs are predominant in the epidemiologic studies, but rather with a conjunction of factors in the course of infection including environmental risk and parasite and host factors.
Asunto(s)
Infecciones por Blastocystis/epidemiología , Infecciones por Blastocystis/parasitología , Blastocystis/clasificación , Blastocystis/genética , Adolescente , Adulto , Blastocystis/aislamiento & purificación , Niño , Análisis por Conglomerados , ADN Protozoario/química , ADN Protozoario/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Egipto/epidemiología , Heces/parasitología , Femenino , Gastroenteritis/epidemiología , Gastroenteritis/parasitología , Genes de ARNr , Humanos , Masculino , Persona de Mediana Edad , Epidemiología Molecular , Datos de Secuencia Molecular , Filogenia , ARN Protozoario/genética , ARN Ribosómico 18S/genética , Análisis de Secuencia de ADN , Adulto JovenRESUMEN
A library of 19 novel 4-(4-phenylpiperazine-1-yl)benzamidines has been synthesized and evaluated in vitro against Pneumocystis carinii. Among these compounds, N-ethyl- and N-hexyl-4-(4-phenylpiperazine-1-yl)benzamidines emerged as the most promising compounds, with inhibition percentages at 10.0 microg/mL of 87% and 96%, respectively. Those compounds remained active at 0.1 microg/mL.
Asunto(s)
Antifúngicos/química , Antifúngicos/farmacología , Benzamidinas/química , Benzamidinas/farmacología , Pneumocystis carinii/efectos de los fármacos , Antifúngicos/síntesis química , Benzamidinas/síntesis química , Estructura MolecularRESUMEN
A new Tetratrichomonas species was identified by molecular and phylogenetic approaches in the pleural fluid from a patient with encysted empyema leading to dyspnea. This observation raised the questions of the real prevalence of pulmonary trichomonosis in humans, the zoonotic potential of trichomonads, and the existence of human-host-adapted strains.
Asunto(s)
Empiema Pleural/parasitología , Infecciones por Protozoos/diagnóstico , Infecciones por Protozoos/parasitología , Trichomonadida/clasificación , Trichomonadida/aislamiento & purificación , Adulto , Animales , Análisis por Conglomerados , ADN Protozoario/química , ADN Protozoario/genética , ADN Ribosómico/química , ADN Ribosómico/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Femenino , Genes de ARNr , Humanos , Datos de Secuencia Molecular , Filogenia , ARN Protozoario/genética , ARN Ribosómico 5.8S/genética , Radiografía Torácica , Análisis de Secuencia de ADN , Trichomonadida/genéticaRESUMEN
The separation of Pneumocystis carinii life-cycle stages while preserving infectivity is a hitherto unresolved challenge. We describe an original, reproducible, and efficient method for separating trophic from cystic forms of P. carinii using a high-speed cell sorter. The large amounts of highly purified (99.6+/-0.3%) infectious trophic and cystic forms can now be used to elucidate the poorly understood P. carinii life cycle.