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1.
Vet Parasitol ; 196(1-2): 179-83, 2013 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-23523013

RESUMEN

To analyse genetic variability and population structure, 84 isolates of Echinococcus granulosus (Cestoda: Taeniidae) collected from various host species at different sites of the Tibetan plateau in China were sequenced for the whole mitochondrial nad1 (894 bp) and atp6 (513 bp) genes. The vast majority were classified as G1 genotype (n=82), and two samples from human patients in Sichuan province were identified as G3 genotype. Based on the concatenated sequences of nad1+atp6, 28 different haplotypes (NA1-NA28) were identified. A parsimonious network of the concatenated sequence haplotypes showed star-like features in the overall population, with NA1 as the major haplotype in the population networks. By AMOVA it was shown that variation of E. granulosus within the overall population was the main pattern of the total genetic variability. Neutrality indexes of the concatenated sequence (nad1+atp6) were computed by Tajima's D and Fu's Fs tests and showed high negative values for E. granulosus, indicating significant deviations from neutrality. FST and Nm values suggested that the populations were not genetically differentiated.


Asunto(s)
ADN Mitocondrial/genética , Equinococosis/veterinaria , Echinococcus granulosus/genética , Variación Genética , Altitud , Animales , Demografía , Equinococosis/epidemiología , Equinococosis/parasitología , Echinococcus granulosus/fisiología , Haplotipos , Humanos , Tibet/epidemiología
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