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1.
Prev Med ; 91: 383-388, 2016 10.
Artículo en Inglés | MEDLINE | ID: mdl-27658650

RESUMEN

BACKGROUND: Building on evidence that natural environments (e.g. parks, woodlands, beaches) are key locations for physical activity, we estimated the total annual amount of adult recreational physical activity in England's natural environments, and assessed implications for population health. METHODS: A cross-sectional analysis of six waves (2009/10-2014/5) of the nationally representative, Monitor of Engagement with the Natural Environment survey (n=280,790). The survey uses a weekly quota sample, and population weights, to estimate nature visit frequency across England, and provides details on a single, randomly selected visit (n=112,422), including: a) duration; b) activity; and c) environment type. RESULTS: Approximately 8.23 million (95% CIs: 7.93, 8.54) adults (19.5% of the population) made at least one 'active visit' (i.e. ≥30min, ≥3 METs) to natural environments in the previous week, resulting in 1.23 billion (1.14, 1.32) 'active visits' annually. An estimated 3.20 million (3.05, 3.35) of these also reported meeting recommended physical activity guidelines (i.e. ≥5×30min a week) fully, or in part, through such visits. Active visits by this group were associated with an estimated 109,164 (101,736, 116,592) Quality Adjusted Life Years (QALYs) annually. Assuming the social value of a QALY to be £20,000, the annual value of these visits was approximately £2.18 billion (£2.03, £2.33). Results for walking were replicated using WHO's Health Economic Assessment Tool. CONCLUSIONS: Natural environments provide the context for a large proportion of England's recreational physical activity and highlight the need to protect and manage such environments for health purposes.


Asunto(s)
Ambiente , Ejercicio Físico , Parques Recreativos/estadística & datos numéricos , Recreación/fisiología , Estudios Transversales , Inglaterra , Conductas Relacionadas con la Salud , Humanos , Años de Vida Ajustados por Calidad de Vida , Encuestas y Cuestionarios
2.
Public Health ; 129(10): 1383-9, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24099716

RESUMEN

Scientific investigations have progressively refined our understanding of the influence of the environment on human health, and the many adverse impacts that human activities exert on the environment, from the local to the planetary level. Nonetheless, throughout the modern public health era, health has been pursued as though our lives and lifestyles are disconnected from ecosystems and their component organisms. The inadequacy of the societal and public health response to obesity, health inequities, and especially global environmental and climate change now calls for an ecological approach which addresses human activity in all its social, economic and cultural complexity. The new approach must be integral to, and interactive, with the natural environment. We see the continuing failure to truly integrate human health and environmental impact analysis as deeply damaging, and we propose a new conceptual model, the ecosystems-enriched Drivers, Pressures, State, Exposure, Effects, Actions or 'eDPSEEA' model, to address this shortcoming. The model recognizes convergence between the concept of ecosystems services which provides a human health and well-being slant to the value of ecosystems while equally emphasizing the health of the environment, and the growing calls for 'ecological public health' as a response to global environmental concerns now suffusing the discourse in public health. More revolution than evolution, ecological public health will demand new perspectives regarding the interconnections among society, the economy, the environment and our health and well-being. Success must be built on collaborations between the disparate scientific communities of the environmental sciences and public health as well as interactions with social scientists, economists and the legal profession. It will require outreach to political and other stakeholders including a currently largely disengaged general public. The need for an effective and robust science-policy interface has never been more pressing. Conceptual models can facilitate this by providing theoretical frameworks and supporting stakeholder engagement process simplifications for inherently complex situations involving environment and human health and well-being. They can be tools to think with, to engage, to communicate and to help navigate in a sea of complexity. We believe models such as eDPSEEA can help frame many of the issues which have become the challenges of the new public health era and can provide the essential platforms necessary for progress.


Asunto(s)
Ecosistema , Salud Pública , Cambio Climático , Humanos
3.
Microb Ecol ; 65(4): 852-9, 2013 May.
Artículo en Inglés | MEDLINE | ID: mdl-23325465

RESUMEN

Early steps in the emergence of the discipline of "Oceans and Human Health" are charted in the USA and discussed in relation to past and present marine environment and human health research activities in Europe. Differences in terminology are considered, as well as differences in circumstances related to the various seas of Europe and the intensity of human coastal activity and impact. Opportunities to progress interdisciplinary research are described, and the value of horizon scanning for the early identification of emerging issues is highlighted. The challenges facing researchers and policymakers addressing oceans and human health issues are outlined and some suggestions offered regarding how further progress in research and training into both the risks and benefits of Oceans and Human Health might be made on both sides of the Atlantic.


Asunto(s)
Ecosistema , Actividades Humanas , Agua de Mar/química , Animales , Salud , Humanos , Océanos y Mares
4.
Environ Sci Technol ; 45(11): 4660-5, 2011 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-21504154

RESUMEN

Exposure of individuals to natural environments, such as forests and coastlines, can promote stress reduction and assist in mental recovery following intensive cognitive activities. Settings as simple as hospital window views onto garden-like scenes can also be influential in reducing patients' postoperative recovery periods and analgesic requirements. This paper reviews the evidence supporting the exploitation of these restorative natural environments in future healthcare strategies. The paper also describes early research addressing the development of multisensory, computer-generated restorative environments for the benefit of patients with a variety of psychologically related conditions (including depression, attention deficit disorder, pain, and sleep deficit), who may be unable to access and experience real natural environments, such as those in hospices, military rehabilitation centers, and long-term care facilities. The Table of Contents art is a virtual reconstruction of Wembury Bay, in the southwest of the UK, based on imported Digital Terrain Elevation Data (DTED) to provide the topography and a high-resolution aerial image to provide a template for the location of 3D building and vegetation models, rock features, and pathways. The 3D environment is rendered using the Unity 3 Game Development Tool and includes spatial sound effects (waves, wind, birdsong, etc.), physics-based features (such as early morning sea mist), time-of-day cycles, and real-time weather changes. The Village Church of St. Werburgh can also be seen in this image.


Asunto(s)
Ambiente , Salud , Naturaleza , Interfaz Usuario-Computador , Humanos
5.
Environ Sci Technol ; 45(5): 1761-72, 2011 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-21291246

RESUMEN

Our objective was to compare the effects on mental and physical wellbeing, health related quality of life and long-term adherence to physical activity, of participation in physical activity in natural environments compared with physical activity indoors. We conducted a systematic review using the following data sources: Medline, Embase, Psychinfo, GreenFILE, SportDISCUS, The Cochrane Library, Science Citation Index Expanded, Social Sciences Citation Index, Arts and Humanities Citation Index, Conference Proceedings Citation Index--Science and BIOSIS from inception to June 2010. Internet searches of relevant Web sites, hand searches of relevant journals, and the reference lists of included papers and other review papers identified in the search were also searched for relevant information. Controlled trials (randomized and nonrandomized) were included. To be eligible trials had to compare the effects of outdoor exercise initiatives with those conducted indoors and report on at least one physical or mental wellbeing outcome in adults or children. Screening of articles for inclusion, data extraction, and quality appraisal were performed by one reviewer and checked by a second with discrepancies resolved by discussion with a third if necessary. Due to the heterogeneity of identified studies a narrative synthesis was performed. Eleven trials (833 adults) were included. Most participants (6 trials; 523 adults) were young students. Study entry criteria and methods were sparsely reported. All interventions consisted of a single episode of walking or running indoors with the same activity at a similar level conducted outdoors on a separate occasion. A total of 13 different outcome measures were used to evaluate the effects of exercise on mental wellbeing, and 4 outcome measures were used to assess attitude to exercise. Most trials (n = 9) showed some improvement in mental wellbeing on one or other of the outcome measures. Compared with exercising indoors, exercising in natural environments was associated with greater feelings of revitalization and positive engagement, decreases in tension, confusion, anger, and depression, and increased energy. However, the results suggested that feelings of calmness may be decreased following outdoor exercise. Participants reported greater enjoyment and satisfaction with outdoor activity and declared a greater intent to repeat the activity at a later date. None of the identified studies measured the effects of physical activity on physical wellbeing or the effect of natural environments on exercise adherence. The hypothesis that there are added beneficial effects to be gained from performing physical activity outdoors in natural environments is very appealing and has generated considerable interest. This review has shown some promising effects on self-reported mental wellbeing immediately following exercise in nature which are not seen following the same exercise indoors. However, the interpretation and extrapolation of these findings is hampered by the poor methodological quality of the available evidence and the heterogeneity of outcome measures employed. The review demonstrates the paucity of high quality evidence on which to base recommendations and reveals an undoubted need for further research in this area. Large, well designed, longer term trials in populations who might benefit most from the potential advantages of outdoor exercise are needed to fully elucidate the effects on mental and physical wellbeing. The influence of these effects on the sustainability of physical activity initiatives also awaits investigation.


Asunto(s)
Estado de Salud , Salud Mental/estadística & datos numéricos , Actividad Motora , Recreación , Adulto , Ejercicio Físico , Humanos
6.
Sci Total Environ ; 372(2-3): 515-31, 2007 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-17140642

RESUMEN

The investigation aimed primarily at understanding the PAH record in inter-tidal mussel tissues and evaluating the bivalve performance as a bioindicator for oil contamination. The species Perna perna was used as test organism since it is abundantly distributed in coastal areas of the Americas, Africa and elsewhere. The study was carried out in Guanabara Bay and comprised two observation phases: phase one included seasonal sampling in 8 sites, whereas in phase two 4 sites were examined over 4 years. Among the 35 determined PAH (60-90 microg kg(-1) up to 4000-6000 microg kg(-1)) alkylated homologues predominated by more than 80%. The PAH profile in inter-tidal mussels is largely petrogenic with high contribution of dibenzothiophenes (DBT) and phenanthrenes (Ph). The prevailing petrogenic fingerprint, confirmed by diagnostic ratios, is linked to the properties of the inter-tidal habitat, which favors exposure to oil films. C2DBT/C2Ph and C3DBT/C3Ph ratios, however, show a wide range of values uncorrelated to specific oils. Micronucleus frequencies are significantly related to PAH concentrations, especially to those of alkylated homologues. Genotoxic expression appears at concentrations as low as 300 microg kg(-1) summation operator 35 PAH.


Asunto(s)
Monitoreo del Ambiente/métodos , Perna/química , Hidrocarburos Policíclicos Aromáticos/análisis , Contaminantes Químicos del Agua/análisis , Animales , Brasil , Análisis Factorial , Pruebas de Micronúcleos , Pruebas de Mutagenicidad , Hidrocarburos Policíclicos Aromáticos/toxicidad , Factores de Tiempo , Contaminantes Químicos del Agua/toxicidad
7.
Mar Pollut Bull ; 54(3): 329-38, 2007 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-17196223

RESUMEN

The mussel Perna perna was used to monitor and assess areas suspected of oil contamination by transplanting animals from unimpacted to impacted sites and vice versa. The deployment lasted for three months during which total PAH were measured in seawater samples collected every 15 days. PAH concentrations were determined in mussel tissue monthly. The lysosomal neutral red assay and the condition index were used as biomarkers of the adverse biological effects. The PAH concentration in mussels at unimpacted sites, prior to deployment was ca. 100 microg kg(-1) but increased to 300 microg kg(-1) after three months at the contaminated site. Mussels transplanted to the uncontaminated site showed a decrease from 380 to 80 microg kg(-1). Significant changes in lysosomal stability occurred within one month of the beginning of the transposition; in contrast condition indexes were not correlated with the observed changes in PAH concentration.


Asunto(s)
Monitoreo del Ambiente/métodos , Perna/efectos de los fármacos , Hidrocarburos Policíclicos Aromáticos/análisis , Hidrocarburos Policíclicos Aromáticos/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad , Animales , Agua de Mar/química
10.
Int J Radiat Oncol Biol Phys ; 9(2): 145-51, 1983 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-6339447

RESUMEN

Results of a prospective lung function study are presented for 48 patients with acute myeloid leukemia (AML) treated with total body irradiation (TBI) and bone marrow transplantation (BMT) at the Royal Marsden Hospital between 1978 and 1980. Patients with active disease or who were in remission following cytoreductive chemotherapy had mildly impaired gas exchange prior to grafting. After TBI and BMT all patients studied developed progressive deterioration of lung function during the first 100 days, although these changes were subclinical. Infection and graft-versus-host disease (GvHD) were associated with further worsening of restrictive ventilatory defects and diffusing capacity (DLCO). Beyond 100 days, ventilatory ability returned to normal and gas transfer improved, although it failed to reach pre-transplant levels. There was no evidence of progressive pulmonary fibrosis during the first year after grafting.


Asunto(s)
Trasplante de Médula Ósea , Leucemia Mieloide Aguda/terapia , Pulmón/fisiopatología , Adolescente , Adulto , Niño , Femenino , Humanos , Leucemia Mieloide Aguda/fisiopatología , Leucemia Mieloide Aguda/radioterapia , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Fibrosis Pulmonar/etiología , Traumatismos por Radiación/etiología , Pruebas de Función Respiratoria , Irradiación Corporal Total
11.
Int J Radiat Oncol Biol Phys ; 9(7): 1029-33, 1983 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-6305894

RESUMEN

Idiopathic and infective interstitial pneumonitis (IPn) is a common complication after bone marrow transplantation (BMT) in many centers and carries a high mortality. We report here a series of 107 patients with acute leukemia grafted at the Royal Marsden Hospital in which only 11 (10.3%) developed IPn and only 5 died (5%). Only one case of idiopathic IPn was seen. Factors which may account for this low incidence are discussed. Sixty of 107 patients were transplanted in first remission of acute myeloid leukemia (AML) and were therefore in good general condition. Lung radiation doses were carefully monitored and doses of 10.5 Gy were not exceeded except in a group of 16 patients in whom a study of escalating doses of TBI (up to 13 Gy) was undertaken. The dose rate used for total body irradiation (TBI) was lower than that used in other centers and as demonstrated elsewhere by ourselves and others, reduction of dose rate to less than 0.05 Gy/min may be expected to lead to substantial reduction in lung damage. Threshold doses of approximately 8 Gy for IPn have been reported, but within the dose range of 8 to 10.5 Gy we suggest that dose rate may significantly affect the incidence. Data so far available suggest a true improvement in therapeutic ratio for low dose rate single fraction TBI compared with high dose rate.


Asunto(s)
Trasplante de Médula Ósea , Leucemia/terapia , Neumonía por Pneumocystis/etiología , Fibrosis Pulmonar/etiología , Irradiación Corporal Total/efectos adversos , Enfermedad Aguda , Adolescente , Adulto , Niño , Infecciones por Citomegalovirus/etiología , Femenino , Herpes Simple/etiología , Humanos , Masculino , Persona de Mediana Edad , Dosificación Radioterapéutica , Estudios Retrospectivos
12.
Environ Health Perspect ; 102 Suppl 12: 101-4, 1994 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-7713024

RESUMEN

The goals of genetic ecotoxicology are discussed and redefined. New directions in which genotoxicity "effect" studies might be pursued are outlined. Recognition of the genotoxic disease syndrome in lower animals suggests that more attention should be given to exploring the relationships between DNA damage (adduct formation, gene mutations, etc.) and its manifestation at the level of individuals. Within a given population, not all individuals are equally susceptible to pollutant toxicity (including genotoxicity). It is proposed therefore, that more attention be paid to identifying the factors underlying interindividual variability in susceptibility. Examples are provided of specific cases in which differences in susceptibility to pollutants have been directly related to genotypic predisposition. This approach is also advocated for investigating the individual and population level consequences of genotoxic damage. The possibility of using phenotypic traits to recognise subsets of individuals within populations possessing similar genotypes is discussed.


Asunto(s)
Daño del ADN/efectos de los fármacos , Contaminantes Ambientales/efectos adversos , Genética de Población/efectos de los fármacos , Animales , Braquiuros , Aductos de ADN/efectos adversos , Daphnia , Drosophila , Monitoreo del Ambiente , Genotipo , Mutágenos/efectos adversos , Fenotipo
13.
Environ Health Perspect ; 105 Suppl 4: 849-54, 1997 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-9255571

RESUMEN

Environmental management is inevitably complicated by the large variation in susceptibility to chemical toxicity exhibited by the living components of ecosystems, a significant proportion of which is determined by genetic factors. This paper examines the concept of genetic susceptibility in ecosystems and suggests the existence of two distinct forms reflecting genetic changes at the level of the individual and at the level of population and community. The influence of genetic susceptibility on exposure-response curves is discussed and the consequent accuracy of data used for toxicity test-based risk assessments examined. The paper concludes by describing a possible biomarker-based approach to future studies of susceptibility in ecosystems, suggesting the use of modern molecular genetic methods.


Asunto(s)
Ecosistema , Sustancias Peligrosas/toxicidad , Animales , Biomarcadores , Variación Genética , Humanos
14.
J Clin Pathol ; 36(5): 546-54, 1983 May.
Artículo en Inglés | MEDLINE | ID: mdl-6341414

RESUMEN

The histopathological changes in the lungs of 32 patients who died after bone marrow transplantation for leukaemia have been studied and compared with those found in 21 patients treated by conventional chemotherapy. The transplanted patients exhibited a higher incidence of interstitial pneumonitis, vascular lesions and viral infections, particularly cytomegalovirus (CMV), although bacterial and fungal diseases were commoner in the non-grafted subjects. The pathogenesis of interstitial pneumonitis is discussed with specific reference to the possible roles of irradiation, chemotherapy, viruses and the immunosuppressive drug cyclosporin A. Ten patients died of a syndrome characterised clinically by fever, skin rash, fluid retention, uraemia, low serum albumin concentrations, low central venous pressure and acute pulmonary oedema. These patients exhibited intra-alveolar haemorrhagic fibrinous exudation with or without interstitial changes. The aetiology of this syndrome is not known but it occurs more frequently in recipients of mismatched grafts and evidence is presented suggesting that viruses may play a significant causative role. No lesion was identified that could be directly attributed to Graft-versus-Host disease.


Asunto(s)
Trasplante de Médula Ósea , Leucemia/terapia , Enfermedades Pulmonares/patología , Pulmón/patología , Adolescente , Adulto , Niño , Femenino , Rechazo de Injerto , Humanos , Pulmón/irrigación sanguínea , Enfermedades Pulmonares/etiología , Masculino , Persona de Mediana Edad , Edema Pulmonar/etiología , Edema Pulmonar/patología , Fibrosis Pulmonar/etiología , Fibrosis Pulmonar/patología , Enfermedades Vasculares/etiología , Enfermedades Vasculares/patología
15.
Toxicol Sci ; 59(2): 241-50, 2001 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-11158717

RESUMEN

This study compared the effects occurring at molecular and population levels in Daphnia magna exposed to copper concentrations in the range of 15-120 microg/l. The qualitative and quantitative modifications arising in random amplified polymorphic DNA (RAPD) profiles as a measure of DNA effects were compared with a number of key ecological fitness parameters, namely, the age-specific survival, age-specific fecundity, net reproductive rate, and intrinsic rate of population increase. Results suggested that growth, reproduction, and most of the fitness parameters as well as genomic template stability (a qualitative measure reflecting changes in RAPD profiles) were significantly affected at copper concentrations of 90 and 120 microg/l. Among the fitness parameters, the age-specific fecundity and net reproductive rate were the most sensitive parameters of toxicity. Changes in RAPD patterns generally occurred at copper concentrations of 90 and 120 microg/l, but with one primer, changes significantly arose at all copper concentrations. Overall, molecular and population parameters compared well and represented a sensitive means to measure toxicity induced by copper in Daphnia magna. In conclusion, the measurement of parameters at both molecular and population levels is valuable for investigating the specific effects of agents interacting with DNA. Ultimately, this methodology may allow the ecotoxicological examination of the link between molecular alterations and measurable adverse effects at higher levels of biological organization.


Asunto(s)
Cobre/toxicidad , ADN/efectos de los fármacos , Daphnia/efectos de los fármacos , Dinámica Poblacional , Animales , ADN/análisis , Daphnia/genética , Daphnia/crecimiento & desarrollo , Relación Dosis-Respuesta a Droga , Genómica , Dosificación Letal Mediana , Técnica del ADN Polimorfo Amplificado Aleatorio , Reproducción/efectos de los fármacos
16.
Mutat Res ; 399(1): 109-22, 1998 Mar 13.
Artículo en Inglés | MEDLINE | ID: mdl-9635493

RESUMEN

Attention is drawn to the goals of genetic ecotoxicology, in particular, the need to relate genotoxicity in individuals to population and community level consequences. The evidence for pollutant-induced genotoxicity in marine invertebrates is reviewed. Neoplasia is apparently rare in marine invertebrates and only limited evidence is available to suggest that chemical genotoxins act as causative agents. It is unknown why marine invertebrates exhibit low tumour incidences and are much more tolerant of ionising radiation than their vertebrate counterparts. The importance of the genotoxic disease syndrome is highlighted. Disentangling phenotypic manifestations of genotoxic damage and that due to direct metabolic toxicity provides a major challenge for the future. Further work is required to assess the significance of interspecific and interindividual variability in susceptibility to genotoxicity, especially with regard to the evolution of resistant populations and communities of marine organisms at contaminated sites. Only by addressing the issues highlighted above can proper risk assessments of genotoxic agents be performed to minimise threats to human and ecosystem health.


Asunto(s)
Invertebrados/efectos de los fármacos , Mutágenos/toxicidad , Contaminantes del Agua/toxicidad , Animales , Ecología
17.
Mutat Res ; 464(2): 213-28, 2000 Jan 24.
Artículo en Inglés | MEDLINE | ID: mdl-10648908

RESUMEN

In genetic ecotoxicology or eco-genotoxicology, there is lack of well-validated systems which could demonstrate the utility of multiple endpoints in environmental quality assessment. For an evaluation of genotoxic potential of heterogeneous marine sediment samples collected from a small fishing harbour in the UK, an in vivo test system using embryo-larval stages of the common mussel, Mytilus edulis was validated against direct and indirect acting reference mutagens. The system appeared to be sensitive and reproducible for cytogenetic endpoints analysed (sister chromatid exchanges (SCEs) and chromosomal aberrations (CAbs)). Following validation and chemical characterisation of the environmental samples, multiple endpoints were measured. Determination of the maximum tolerated dose (MTD) was carried out as a measure to determine cytotoxic effects as a confounding factor for genotoxicity, based on developmental and cytotoxic (in terms of proliferative rate index or PRI) effects. Evaluation of the genotoxic potential of the samples gave a positive response for all the endpoints tested, linking different levels of biological organisation (i.e., chromosomal, cellular and organismal) for the observed effects. The study also emphasises the need for the assessment of the short and long-term impacts of dredge disposal on marine biota by including laboratory-based bioassays and incorporating an integrated approach which could yield as much useful information as possible in overall hazard and risk assessment for aquatic genotoxicity.


Asunto(s)
Bioensayo/métodos , Bivalvos/embriología , Monitoreo del Ambiente/métodos , Mutágenos/análisis , Contaminación Química del Agua/análisis , Animales , Aberraciones Cromosómicas , Relación Dosis-Respuesta a Droga , Embrión no Mamífero/efectos de los fármacos , Sedimentos Geológicos/química , Concentración de Iones de Hidrógeno , Cariotipificación , Larva/efectos de los fármacos , Metales Pesados/análisis , Pruebas de Mutagenicidad/métodos , Mutágenos/toxicidad , Oxidación-Reducción , Oxígeno/análisis , Tamaño de la Partícula , Reproducibilidad de los Resultados , Intercambio de Cromátides Hermanas/efectos de los fármacos
18.
Comp Biochem Physiol B Biochem Mol Biol ; 125(4): 483-91, 2000 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-10904861

RESUMEN

The presence of nitric oxide synthase (EC 1.14.23 NOS) activity is demonstrated in the tropical marine cnidarian Aiptasia pallida (Verrill). Enzyme activity was assayed by measuring the conversion of [3H]arginine to [3H]citrulline. Optimal NOS activity was found to require NADPH. Activity was inhibited by the competitive NOS inhibitor NG-methyl-L-arginine (L-NMA), but not the arginase inhibitors L-valine and L-ornithine. NOS activity was predominantly cytosolic, and was characterised by a Km for arginine of 19.05 microM and a Vmax of 2.96 pmol/min per microgram protein. Histochemical localisation of NOS activity using NADPH diaphorase staining showed the enzyme to be predominantly present in the epidermal cells and at the extremities of the mesoglea. These results provide a preliminary biochemical characterisation and histochemical localisation of NOS activity in A. pallida, an ecologically important sentinel species in tropical marine ecosystems.


Asunto(s)
Óxido Nítrico Sintasa/metabolismo , Anémonas de Mar/enzimología , Animales , Arginina/análogos & derivados , Arginina/farmacología , Citrulina/análisis , Citrulina/metabolismo , Inhibidores Enzimáticos/farmacología , Células Epiteliales/enzimología , NADP/metabolismo , NADPH Deshidrogenasa/análisis , NADPH Deshidrogenasa/metabolismo , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico Sintasa/antagonistas & inhibidores , Anémonas de Mar/fisiología , Coloración y Etiquetado/métodos , Valina/farmacología
19.
Aquat Toxicol ; 51(3): 277-91, 2001 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-11090890

RESUMEN

Numerous studies have indicated that stress proteins have potential as biomarkers of exposure to environmental contaminants. Analysis of stress proteins in animals, especially Hsp70, has dominated this research area despite increasing interest in the use of plants as pollution monitors. In the present study Hsp70 expression in Enteromorpha intestinalis has been investigated under exposure to a variety of stressors, to evaluate the potential use of Hsp70 as a biomarker of contaminant exposure in this seaweed. E. intestinalis was exposed to thermal stress, copper under differing conditions of nutrient availability and the triazine herbicide Irgarol 1051. Measurements were also taken to compare Hsp70 with conventional sublethal endpoints of toxicity such as growth and fluorescence induction parameters such as Fv/Fm. E. intestinalis exhibited a typical heat shock response. Hsp70 expression was increased with copper exposure, but proved to be a relatively insensitive biomarker of copper exposure compared to growth measurements. Nutrient limitation enhanced copper toxicity and significantly impaired growth, Fv/Fm and Hsp70 production. Fv/Fm and growth were strongly affected by Irgarol 1051 exposure, but Hsp70 levels were unaltered following exposure to the herbicide. The implications of using Hsp70 expression in E. intestinalis as a biomarker are discussed.


Asunto(s)
Chlorophyta/metabolismo , Proteínas HSP70 de Choque Térmico/biosíntesis , Calor/efectos adversos , Chlorophyta/efectos de los fármacos , Chlorophyta/crecimiento & desarrollo , Cobre/toxicidad , Herbicidas/toxicidad , Fotosíntesis/efectos de los fármacos , Fotosíntesis/fisiología , Triazinas/toxicidad
20.
Aquat Toxicol ; 52(1): 29-38, 2001 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-11163428

RESUMEN

Previous studies have shown that low levels of copper (down to 0.8 microM) induce bradycardia in the blue mussel (Mytilus edulis) and that this is not caused by prolonged valve closure. The aim of this study was to determine the precise mechanism responsible. To establish if copper was directly affecting heart cell physiology, recordings of contractions from isolated ventricular strips were made using an isometric force transducer, in response to copper concentrations (as CuCl2) ranging between 1 microM and 1 mM. Inhibition of mechanical activity only occurred at 1 mM copper, suggesting that the copper-induced bradycardia observed in whole animals cannot be attributed to direct cardiotoxicity. Effects of copper on the cardiac nerves were subsequently examined. Following removal of visceral ganglia (from where the cardiac nerves originate), exposure to 12.5 microM copper had no effect on the heart rate of whole animals. The effect of copper on the heart rate of mussels could not be abolished by depletion of the monoamine content of the animal using reserpine. However, pre-treatment of the animals with alpha-bungarotoxin considerably reduced the sensitivity of the heart to copper. These results indicated that the influence of copper on the heart of M. edulis might be mediated by a change in the activity of cholinergic nerves to heart. In the final experiments, mussels were injected with either benzoquinonium or D-tubocurarine, prior to copper exposure, in an attempt to selectively block the inhibitory or excitatory cholinoreceptors of the heart. Only benzoquinonium decreased the susceptibility of the heart to copper, suggesting that copper affects the cardiac activity of blue mussels by stimulating inhibitory cholinergic nerves to the heart.


Asunto(s)
Bivalvos/fisiología , Cobre/toxicidad , Corazón/efectos de los fármacos , Corazón/fisiología , Animales , Frecuencia Cardíaca/efectos de los fármacos , Miocardio/metabolismo , Antagonistas Nicotínicos/farmacología , Reserpina/farmacología
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