[Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100ß protein and myelin basic protein in rats following status epilepticus].

OBJECTIVE: This study examined the effect of recombinant human erythropoietin (r-HuEPO) on the serum levels of neuron-specific enolase (NSE), S-100ß protein and myelin basic protein (MBP) in young rats 24 hrs after lithium-pilocarpine-induced status epilepticus (SE) in order to study the potential role of r-HuEPO in epileptic brain damage. METHODS: Forty 19-21-day-old male Sprague-Dawley (SD) rats were randomly divided into four groups (n=10): normal control group, SE, r-HuEPO pretreated-SE and r-HuEPO. SE was induced by lithium-pilocarpine. R-HuEPO (500 IU/kg) was intraperitoneally injected in the r-HuEPO pretreated-SE and r-HuEPO groups 4 hrs before SE. Serum levels of NSE, S-100ß and MBP were determined 24 hrs after the SE event. RESULTS: Serum levels of NSE, S-100ß and MBP in the SE group increased significantly compared with those in the normal control and the r-HuEPO groups (P＜0.05). The r-HuEPO pretreated-SE group showed significantly decreased serum levels of NSE, S-100ß and MBP compared with the SE group (P＜0.05). CONCLUSIONS: r-HuEPO may reduce the expression of NSE, S-100ß and MBP and thus might provide an early protective effect against epileptic brain injury.

Plasma copeptin levels are associated with prognosis of severe acute pancreatitis.

Copeptin reflects the individual stress level, and is correlated with outcomes of critical illness. This study was designed to evaluate its relationship with disease severity, local complications, organ failure and mortality of severe acute pancreatitis (SAP). Seventy-eight SAP patients and 78 sex- and age-matched healthy individuals were recruited. Plasma samples were obtained on admission from SAP patients and at study entry from healthy individuals. Copeptin concentration was determined using enzyme-linked immunosorbent assay. Plasma copeptin level was obviously higher in patients than in healthy individuals, was identified as an independent predictor of local complications, organ failure and in-hospital mortality, was highly associated with traditional predictors of disease severity and mortality including the Acute Physiology and Chronic Health Care Evaluation II score, Ranson score, multiple organ dysfunction score, sequential organ failure assessment score, and predicted local complications, organ failure, and in-hospital mortality of SAP patients with high areas under receiver operating characteristic curve. Furthermore, its predictive value was similar to the traditional predictors'. However, it could not improve these traditional predictors' predictive values. Therefore, increased plasma copeptin level is associated with disease severity and identified as a novel prognostic marker of local complications, organ failure and mortality after SAP.

Relationship between plasma copeptin levels and complications of community-acquired pneumonia in preschool children.

High plasma copeptin level has been associated with clinical outcomes after acute illness. The present study was undertaken to investigate the plasma copeptin concentrations in preschool children with community-acquired pneumonia (CAP) and to analyze the correlations of copeptin with CAP-related complications and pleural effusion. Plasma copeptin concentrations of 100 healthy children and 165 preschool children with CAP were measured. 35 children (21.2%) presented with complicated CAP and 28 children (17.0%) presented with pleural effusion. The admission copeptin levels were significantly increased in all patients (49.7 ± 21.4 pmol/L), children with complicated CAP (73.0 ± 16.9 pmol/L), those with uncomplicated CAP (43.4 ± 17.8 pmol/L), those with pleural effusion (70.9 ± 17.4 pmol/L) and those without pleural effusion (45.3 ± 19.5 pmol/L) compared with healthy control individuals (9.0 ± 2.7 pmol/L, all P<0.001). Multivariate logistic regression analysis showed that plasma copeptin levels were independently related to CAP-related complications (odds ratio 1.214, 95% confidence interval 1.104-1.872, P<0.001) and pleural effusion (odds ratio 1.226, 95% confidence interval 1.109-1.917, P<0.001). A receiver operating characteristic curve analysis showed plasma copeptin level better predicted CAP-related complications (area under curve 0.876, 95% confidence interval 0.815-0.922) and pleural effusion (area under curve 0.831, 95% confidence interval 0.765-0.885). Thus, plasma copeptin level may represent a novel biomarker for predicting CAP-related complications in preschool children.

[Expression and clinical significance of surfactant protein D mRNA in peripheral blood of lung cancer patients].

BACKGROUND AND OBJECTIVES: The sensitive detection of circulating tumor cells in lung cancer patients is very important for prognosis and selection of appropriate treatment modalities. Based on recent promising data, the authors established reverse transcriptase (RT)-PCR with primers specific for surfactant protein D (SP-D) gene to detect circulating tumor cells in the peripheral blood of lung cancer patients, and to initiatively discuss its clinical significance. METHODS: The expression of SP-D mRNA was analyzed by an improved nested RT-PCR in the peripheral blood of 26 lung cancer patients with metastases, 37 lung cancer patients without metastases, 15 benign pneumonia patients, and 15 healthy volunteers. RESULTS: 1) The sensitivity of the method was 1 x 10(-6), with high level of specificity. 2) Using this method, the positive detection rate of the expression of SP-D mRNA was 92.3% (24/26) and 24.3% (9/37) in the peripheral blood of lung cancer patients with and without metastases, respectively. While no sample was positive for SP-D mRNA expression in the benign pneumonia patients and in the healthy volunteers. CONCLUSIONS: SP-D mRNA might be a valuable marker to detect circulating tumor cells in the peripheral blood of lung cancer patients. This method may be a valuable tool for early identification of metastases in asymptomatic lung cancer patients. Furthermore, it can also provide important clinical information for the evaluation of prognosis and in the selection of appropriate treatment strategies.