[The correlation between reflux esophagitis and Helicobacter pylori infection based on natural population].

Objective: Reflux esophagitis (RE) may be negatively correlated with Helicobacter pylori (H. pylori) infection, but the conclusion and relevant mechanism is still controversial. This study proposed to explore the correlation between RE and H. pylori infection based on natural population. Methods: From July 2013 to December 2014, 3 940 residents aged 40-69 years were recruited in Linqu County of Shandong Province and Hua County of Henan Province by the whole sampling method. All the subjects underwent gastroscopy, and gastric mucosa biopsy specimens were collected for pathological diagnosis and Warthin-Starry (WS) staining to identify H. pylori infection. Venous blood samples of some subjects were collected for H. pylori immunoglobulin G (H. pylori-IgG) detection. Also, demographic and sociological data were collected. Chi-square test and logistic regression were used to analyze the correlation between RE and H. pylori infection. Results: A total of 359 cases of RE were detected. Excluding RE and other upper gastrointestinal organic diseases, 3 382 cases were considered as controls. Chi-square test showed that WS staining positive rate in RE group was significantly lower than that in control group (P=0.023), but there was no significant difference in the positive rate of H. pylori-IgG between the two groups (P=0.281). There were significant differences between RE group and control group in gender composition, age, body mass index (BMI), smoking, alcohol consumption, education level and mucosal active inflammation. Multivariate regression analysis showed that RE was negatively correlated with gastric mucosa active inflammation [OR=0.754 (95%CI 0.600-0.949), P=0.016], and positively correlated with male [OR=4.231 (95%CI 3.263-5.486), P<0.001], age ≥60 years, BMI≥24 kg/m2 [OR=1.540 (95%CI 1.220-1.945), P<0.001]. Compared to those aged 40-49 years and 50-59 years, the odds ratio (OR) of RE in these aged ≥60 years were 1.566 (95%CI 1.144-2.143, P=0.005) and 1.405 (95%CI 1.093-1.805, P=0.008). Conclusion: RE is more closely related to H. pylori present infection. Multivariate analysis showed that RE is negatively correlated with active inflammation of gastric mucosa caused by H. pylori infection, and positively correlated with male, overweight and aged ≥60 years.

[Time trends of H. pylori infection and related drug use in patients with peptic ulcer bleeding in a hospital in Beijing from 2010 to 2019].

Objective: To explore the time trends of H. pylori infection and related drug use in patients with peptic ulcer bleeding (PUB) in recent ten years. Methods: PUB patients in Peking University Third Hospital from 2010 to 2019 were included. Time trends of H. pylori infection, related drugs (non-steroidal anti-inflammatory drugs, aspirin, antiplatelet drugs, anticoagulant drugs, glucocorticoids) use and non-H. pylori-non-drug ulcer in PUB patients were analyzed. Results: A total of 1 140 PUB patients were included from 2010 to 2019 (including 925 males and 215 females). The age M(Q1,Q3)was 53.5 (33.0, 66.0) years, with 833 patients <65 years old. The positive rates of H. pylori were 79.3%, 75.7%, 73.1%, 71.6%, 77.0%, 70.4%, 69.7%, 63.1%, 51.4%, 50.9%, respectively, showing a decreasing trend (χ²=32.386, P<0.001), and the decreasing trends were significant in different ulcer location, age and gender subgroups(all P<0.05). The proportions of PUB patients using at least one of the above drugs were 23.1%, 28.6%, 34.0%, 39.5%, 33.8%, 35.9%, 28.7%, 39.8%, 40.9%, 41.8%, respectively, showing an increasing trend (χ²=6.857, P=0.009), and the proportions of patients with antiplatelet drugs and anticoagulant drugs history showed increasing trends. The proportions of non-H. pylori-non-drug patients were 12.3%, 22.1%, 14.6%, 13.2%, 7.7%, 13.0%, 16.9%, 18.7%, 22.6% and 20.9%, respectively, showing an increasing trend (χ²=4.808, P=0.028). Accordingly, the proportions of elderly (≥65 years old) patients (χ²=4.608, P=0.032) and large ulcer (≥2 cm) patients (χ²=8.173, P=0.004) showed increasing trends. Conclusion: In the last decade, the positive rate of H. pylori in PUB patients showed a decreasing trend, while the proportions of patients with antiplatelet drugs and anticoagulant drugs history and the proportion of non-H. pylori-non-drug patients showed increasing trends.

[Automobile Traffic Accident Death Case Analysis of Characteristics of Driver Injury].

OBJECTIVES: To distinguish the injury characteristic changes on the drivers between the injuries of drivers and passengers in traffic accidents, and to provide scientific evidence for confirming the identity of driver in traffic accidents. METHODS: Data of 126 automobile traffic accident death cases in the reclamation areas of Heilongjiang province from 2006-2014 were retrospectively studied. The injury characteristics on the drivers of automobile traffic accident death cases were analyzed and the forensic identification problem in the injuries of drivers and passengers were discussed. RESULTS: Injuries were frequently observed on driver's neck, chest and abdomen. The characteristic injuries caused by auto parts were also found, which appeared at the places of passenger's head, face and limbs contacted with automobile. Such characteristic injuries were not found at other places. CONCLUSIONS: The location and type of injury are associated with the identity of the deceased.

[Analysis on the Injury Mechanism and Characteristics of Ox Horn].

OBJECTIVES: To study the morphological characteristics of the injury caused by ox horn on human body and its injury mechanism, and to discuss the rules and characteristics of injury by ox horn and provide forensic evidences for identification of such cases. METHODS: The comparative analysis of position and morphological characteristics were performed by summarizing the data of 100 victims gored by ox accepted by Heilongjiang agricultural areas public security bureau during 2004-2014. RESULTS: The specific injuries only could be found at the contact positions such as thorax and abdomen, lower back and limbs of the victims gored by ox horn. Most of the skin wounds had the characteristics of sharp injuries, the bar-type injury by club which called "rail way bruise" was an obvious characteristic appeared on the soft tissue. CONCLUSIONS: Ox horn can cause non-specific injuries on thorax and abdomen, lower back and limbs of human body, which are similar with the characteristics of sharp injury and injury by club. Careful analyzation and identification should be performed on such injury in daily work.

TM4SF5-CTD-2354A18.1-miR-4697-3P may play a key role in the pathogenesis of gastric cancer.

AIMS: Our aim is to identify important lncRNAs and mRNAs which may play a key role in contributing to pathogenesis of gastric cancer. METHODS: Different LncRNAs and mRNAs are identified by microarray in gastric cancer tissue and corresponding normal tissues. The function and relationship of different LncRNAs and mRNAs is performed by GO analysis and Pathway analysis and made code-non-code network (CNC) by Pearson correlation coefficients (PCC). Then mRNA-miRNA relationship is predicted through mRNA-miRNA relationship software (http://www.targetscan.org). Lastly, mRNA-miRNA-LncRNA network is established for further research. RESULTS: The expression profiles of 3732 lncRNAs showed different expression (fold change (FC)≥2.0, p<0.05) in gastric cancer tissue and normal tissue and expression profiles of 3994 mRNAs also showed different expression (FC≥2.0, p<0.05) in gastric cancer and corresponding normal tissue. CONCLUSION: The expression of TM4SF5, CTD-2354A18.1 and miR-4697-3P is in balance at physiological conditions, however, the balance is disrupted by some situations, which may contribute to gastric cancer. GO analysis and Pathway analysis also showed TM4SF5 played an important role in proliferation, differentiation and apoptosis. Therefore, TM4SF5-miR-4697-3P- CTD-2354A18.1 may play a key role in the pathogenesis of gastric cancer (Tab. 2, Fig. 4, Ref. 30).

[Analysis of the efficacy of transnasal endoscopic annulus of zinn area decompression in the treatment of dysthyroid optic neuropathy].

Objective: To evaluate the early efficacy and safety of transnasal endoscopic decompression in the annulus of zinn (AZ) region for refractory dysthyroid optic neuropathy (DON) and to preliminarily analyze the correlated factors of postoperative visual function outcome. Methods: From July 2021 to January 2023, 35 patients (56 eyes) with DON who received AZ area decompression in Peking University Third Hospital were included retrospectively, including 9 males (13 eyes) and 26 females (43 eyes), aging (52.2±12.0) years. Among them, 35 eyes underwent two-wall (medial and inferior) orbital decompression using an endonasal endoscopic approach, while 21 eyes received three-wall (medial, lateral, and inferior) orbital decompression through a combined approach. Key parameters such as best corrected visual acuity (BCVA), visual field (MD value), eyeball prominence, intraocular pressure, and complications were recorded. Postoperative data were collected one month after surgery. The statistical analysis was performed using paired t-test and Spearman correlation analysis. Results: Significant outcomes were observed post surgery in BCVA, visual field, intraocular pressure and proptosis (t value was 8.37, 6.17, 4.50, and 9.20, respectively, all P<0.001). The reduction in proptosis was statistically significant between the 2-wall and 3-wall orbital decompression groups (t=-2.82, P=0.007). Changes in BCVA, visual field, and intraocular pressure before and after surgery was greater in the 3-wall orbital decompression group compared to 2-wall orbital decompression group, although the difference was not statistically significant (all P>0.05). Change in postoperative visual acuity and visual field was significantly positively correlated with preoperative visual acuity and preoperative visual field (all P<0.001). Similarly, change in intraocular pressure and proptosis was positively correlated with preoperative intraocular pressure and preoperative protrusion (all P<0.001). Preoperative diplopia was reported in seven patients (20.0%), and two new cases (5.7%) were noted post-operation, which resolved within 3 months after surgery. Conclusions: Endoscopic endonasal decompression of the AZ area is a safe and effective surgical treatment for DON, with notable improvements in BCVA. Furthermore, three-orbital wall decompression seems to yield better outcomes in terms of eye retraction.

[Quality of life of 22 patients with type 1 Gaucher disease after enzyme replacement therapy].

Objective: To investigate the living status and quality of life (QOL) in type1 Gaucher disease (GD1) patients who underwent long-term enzyme replacement therapy (ERT) and identify possible relevant factors affecting QOL. Methods: Clinical data and SF-36 questionnaires were recorded in 22 adult GD1 patients under regular ERT at Peking Union Medical Colleague Hospital (PUMCH) from January 1995 to June 2017. Results: 13 males and 9 females were included in this study. The current median age, age at diagnosis and initial time of ERT were 41 (24-52) , 6 (1-38) and 26 (6-41) years respectively. Of these patients, 68.2% was living in less-developed regions, 86.4% were under college education, and 77.3% had personal annual income less than ¥30 000 RMB. Though after a median 16 (7-22) years of ERT, the QOL of GD1 patients was still significantly worse (P<0.05) compared with normal Chinese population based on SF-36 questionnaires. History of splenectomy was a negative factor of QOL, mainly in physical health (P<0.05) . Patients could get benefit from early start of ERT in both physical and mental health (P<0.05) . Mental health was not affected by history of splenectomy and related bone diseases. Conclusion: Most adult GD1 patients at PUMCH reside in less-developed regions and have low levels of education and annual income. History of splenectomy and time to start ERT are two important factors affecting QOL. Chinese adult GD1 patients are associated with reduced QOL, even after long-term ERT.

Synthesis of carboxylated chitosan and its adsorption properties for cadmium (II), lead (II) and copper (II) from aqueous solutions.

Carboxylated chitosan (CKCTS) was prepared for the removal of Cd(II), Pb(II), and Cu(II) from aqueous solutions. The effects of experimental parameters such as pH value, initial concentration, contact time and temperature on the adsorption were studied. From the results we can see that the adsorption capacities of Cd(II), Pb(II), and Cu(II) increase with increasing pH of the solution. The kinetic rates were best fitted to the pseudo-second-order model. The adsorption equilibrium data were fitted well with the Langmuir isotherm, which revealed that the maximum adsorption capacities for monolayer saturation of Cd(II), Pb(II), and Cu(II) were 0.555, 0.733 and 0.827 mmol/g, respectively. The adsorption was an exothermic process.

LncRNA SAMD12-AS1 down-regulates P53 to promote malignant progression of glioma.

OBJECTIVE: To study the expression level of long non-coding RNA (LncRNA) SAMD12-AS1 in glioma and its influence on the invasive ability of glioma cells; meanwhile, the regulation mechanism of LncRNA SAMD12-AS1 promoting the development of glioma was further explored. PATIENTS AND METHODS: The expression level of SAMD12-AS1 in 40 pairs of tumor tissue specimens and paracancerous ones collected from glioma patients were examined through Real Time quantitative-Polymerase Chain Reaction (qRT-PCR) method, and the interplay between SAMD12-AS1 expression and clinical indicators of glioma patients was also analyzed. Meanwhile, the expression of SAMD12-AS1 in glioma cell lines was further verified by qRT-PCR. In addition, SAMD12-AS1 knockdown model was constructed using glioma cell lines (T98-G and U87). Cell Counting Kit-8 (CCK-8), cell wound healing test, and transwell assays were conducted to examine the impact of SAMD12-AS on glioma cell functions. Additionally, whether it exerted its biological characteristics through P53 was finally explored. RESULTS: qPCR results in the study revealed that SAMD12-AS1 expression in tumor tissue specimens of glioma patients was remarkably higher than that in the adjacent ones, and the difference was statistically significant. Compared with patients with low expression of SAMD12-AS1, patients with high expression of SAMD1-AS1 had a higher incidence of lymph node or distant metastasis. In addition, compared to the NC group, knocking down SAMD12-AS1 markedly attenuated the proliferation rate, as well as the invasiveness and migration ability of glioma cells. Subsequently, in glioma tissues, it was verified that P53 expression was remarkably decreased and negatively correlated with SAMD12-AS1. Finally, cell recovery experiment also demonstrated that there may exist a mutual regulation between SAMD12-AS1 and P53, which then together affected the malignant progression of glioma. CONCLUSIONS: LncRNA SAMD12-AS1 may accelerate the invasion and migratory capacities of glioma cells by modulating P53, and its expression was confirmed to be significantly relevant to the incidence of lymph node or distant metastasis.

[Late-onset upper airway obstruction caused by alkali inhalation injury: a case report].

SummaryAlkalis can produce severe tissue injury through liquefaction. Since the liquefaction loosens tissue planes and allows deeper penetration of the agent, alkali burns tend to be more severe than acid burns. Alkaline substances ingestion and inhalation frequently causes damage to the upper respiratory and digestive tract. Initial presentation of airway alkali burn includes oropharyngeal pain, dysphagia, hoarseness and stridor. Patients with a clear history of caustic ingestion or inhalation could always receive proper management. In this paper, a middle aged female presented with acute upper airway obstruction was introduced. The cause of the airway compromise was finally determined to be alkali inhalation 3 weeks ago. By reviewing this special case, the clinical features and management of airway alkali burn was summarized.

[The role of body fat rate in the evaluation of obstructive sleep apnea].

Objective: To investigate the role of body fat ratio in the evaluation of obstructive sleep apnea(OSA). Methods: A retrospective analysis was made on 174 cases (between November, 2017 and April, 2018 showed that) of sleep monitoring in the Department of Otorhinolaryngology in Peking University Third Hospital. The data included the gender, age, body fat rate, body mass index (BMI), neck circumference, and apnea-hypopnea index (AHI). The above data were analyzed by non parametric correlation analysis, receiver operating characterristic (ROC) curve analysis and multiple factor Logistic regression analysis to study the relationship between the gender,age,body fat rate,BMI,neck circumference and other indexes of the patients with AHI. Results: Nonparametric correlation analysis showed that the correlation from strong to weak to AHI among women was BMI (r=0.621, P<0.001),body fat rate (r=0.602, P<0.001), age (r=0.570, P<0.001), neck circumference (r=0.402, P=0.014), respectively. BMI (r=0.599, P<0.001), neck circumference (r=0.493, P<0.001), body fat rate (r=0.318, P<0.001), and age (r=0.256, P=0.003) among men. ROC curve analysis showed that the strong to weak index (area under curve,AUC) of the AHI>15/h among women was the body fat rate (AUC=0.884, P=0.001), BMI(AUC=0.810, P=0.008), neck circumference (AUC=0.759, P=0.027), age (AUC=0.750, P=0.033), and the male was BMI (AUC=0.765,P<0.001), neck circumference (AUC=0.720, P<0.001), age (AUC=0.634, P=0.008), and body fat rate (AUC=0.632, P=0.010), respectively. Multifactor Logistic regression analysis showed that the body fat rate (OR=1.704,95%CI=1.012-2.870) in women was an independent risk factor for AHI greater than 15/h; the age of male (OR=1. 044, 95%CI=1.005-1.085) and BMI (OR=1.285, 95%CI=1.056-1.562) were independent risk factors for AHI greater than 15/h. Conclusion: Body fat rate can be used as a new indicator for predicting the severity of OSA,especially in adult female population. In adult female moderate to severe OSA patients (AHI>15/h), compared with BMI,neck circumference and age,the body fat rate has the greatest correlation with AHI. Compared with BMI,neck circumference and age,the body fat rate has a decisive role in predicting moderate to severe OSA (AHI>15/h).

[Effect of sirolimus on erythropoiesis of K562 cell line and patients with pure red cell aplasia in vitro].

Objective: To understand the effect of sirolimus on the erythropoiesis of K562 cell line and bone marrow cells from pure red cell aplasia (PRCA) patients and normal controls. Methods: Different concentrations (10, 100, 1 000 nmol/L) of sirolimus were added to the K562 cell line or bone marrow cells from PRCA patients or normal controls and cultured 14 days for BFU-E formation. Meanwhile, sirolimus was also added to the serum treated PRCA bone marrow cells to cultivate for the same priod of time. Results: Neither K562 cells, bone marrow cells from PRCA patients or normal controls showed any difference when sirolimus was added to the culture system for BFU-E. However, BFU-E formation decreased after serum was added in PRCA patients (76.40±22.48 vs 136.33±12.58, t=-4.329, P=0.001) and this suppression of BFU-E was partly corrected by 1 000 nmol/L sirolimus treatment (97.14±15.83 vs 76.40±22.48, P=0.038). Conclusions: Sirolimus may modulate the suppression of erythropoiesis by serum instead of directly stimulate the growth of red blood cells in PRCA patients.

[Current in eosinophilic otitis media].

Eosinophilic otitis media(EOM) is a kind of intractable disease. It has drawn much attention since it being recognitized first time in 1995. The pathogenesis of EOM remains unknown, and clinical manifestation varies. Early diagnosis and treatment is of great importance for improving the prognosis and avoiding severe hearing loss. This article reviewed current analysis of EOM for better understanding of this disease, and help to establish a standard procedure for the diagnosis and treatment of EOM.

[The relationship between type ⠡ diabetes mellitus and hearing loss：a Meta-analysi].

Objective:Analysis of the relationship between type 2 diabetes and hearing loss through the available literature.Method:According to the main keyword retrieval databases at home and abroad,and in accordance with the inclusion and exclusion criteria for the literature,the listening incidence rate,OR value,the frequency of pure tone hearing threshold,tests the average standard deviation were chose as the evaluation index. Meta analysis was carried out using Rev Man5.3 software. Result:According to the inclusion and exclusion criteria,the articles were screened and included in the final to meet the requirements of the 17 studies. The prevalence of hearing loss in patients with type II diabetes mellitus was 21.0%-74.6%,which was 2.54 times higher than the control group. The difference was statistically significant between the two groups(OR=2.54,95% CI 1.85-3.49,P<0.000 01). In addition,the hearing threshold of all frequencies in type Ⅱ diabetes group was higher than that of control group(Z=5.03,P<0.000 01). Conclusion:Type 2 diabetes can affect hearing loss,especially at the high frequencies.

Synthetic Biology Approaches to New Bisindoles.

Bisindoles are a class of natural products derived from oxidative dimerization of tryptophan, and many of these molecules have potential use as anticancer agents. The recent isolation of new bisindoles and their corresponding gene clusters has greatly expanded the repertoire of biosynthetic genes available to synthetic biologists. This chapter describes methods to exploit the biosynthetic pathways leading to bisindoles, using cladoniamides as a representative example. Specifically, we describe how to identify and heterologously express gene clusters and how to manipulate pathways in order to generate new bisindoles. We also discuss methods for cultivating, extracting, purifying, and characterizing these new metabolites.

Pronounced differences between native Chinese and Swedish populations in the polymorphic hydroxylations of debrisoquin and S-mephenytoin.

The frequency of poor metabolizers of debrisoquin was low and similar in four different native Chinese nationalities. In a total sample of 695 Chinese subjects, only seven (1.01%) had a urinary ratio between debrisoquin and 4-hydroxydebrisoquin greater than 12.6, which is the antimode between poor metabolizers and extensive metabolizers in white populations. This is significantly lower than the 6.82% found in 1011 white Swedish healthy subjects (p less than 0.0001). Admixture analysis indicated the occurrence of two distributions within extensive metabolizers among both Chinese and white subjects. The mean of the distribution of metabolic ratios among Chinese extensive metabolizers was shifted toward higher values compared with Swedish extensive metabolizers (p less than 0.01). The frequency of poor metabolizers of S-mephenytoin was higher in 137 Chinese (14.6%) than in 488 Swedish (3.3%) subjects (p less than 0.0001). Our findings imply that drugs metabolized by these two polymorphic hydroxylases should be prescribed in different dosages to Chinese and white subjects.

Methylmercury-induced alterations in excitatory amino acid transport in rat primary astrocyte cultures.

To determine whether methylmercury (MeHg) has the potential to interfere with homeostatic functions in neonatal rat cortical primary astrocyte cultures, the effects of MeHg on the uptake and efflux of both L-glutamate and D-aspartate were examined. Uptake of both of these excitatory amino acids (EAAs) was significantly (P < 0.05) reduced in the presence of MeHg concentrations as low as 10(-5) M. Efflux of both glutamate and aspartate from preloaded astrocytes was also increased by MeHg in a dose- and time-dependent fashion. Since in our earlier studies we had found that MeHg causes dose-dependent astrocytic swelling, which could have been the mechanism of the increased efflux, we examined whether blockage of conductive ion fluxes, which have been implicated in astrocytic swelling, could reverse the MeHg-induced increase in L-glutamate and D-aspartate efflux. Three compounds which inhibit the hypotonic-media-induced efflux of EAA, 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS), L-644,711 (a derivative of ethacrynic acid) and furosemide were tested at their maximal concentrations for their ability to reverse MeHg-induced EAA efflux. Only furosemide (5 mM) could sustain the reversal for the entire 120 min duration of the efflux measurement. Since hypotonic-media swelling-induced release of EAAs is inhibited by these anion inhibitors (in the following rank order: L-644,711 > SITS > furosemide), we conclude that different mechanisms account for EAA release from primary astrocyte cultures during MeHg exposure as compared to hypotonic media-induced efflux.

The influence of dietary iodine on iodine in the blood serum of cows and calves in the perinatal period.

Concentrations of total iodine (TI) and protein-bound iodine (PBI) in the blood serum of both cows and their neonatal calves were related to the iodine content of the diet fed to the cows. A group of six cows (group 1) fed a diet containing 0.6 ppm iodine had mean concentrations of TI and PBI of 4.1 and 3.4 microgram/100 ml respectively. A second group (group 2), fed a diet containing 4.6 ppm iodine, had concentrations of 33.8 and 7.6 microgram/100 ml of TI and PBI respectively. Calves of groups 1 and 2 had mean concentrations of TI of 21 microgram/100 ml and 96 microgram/100 ml respectively within 24 h of birth, but these concentrations declined markedly over a period of eight days. Evidence was obtained that the high concentrations of TI, and also of PBI, in neonatal calves relative to the concentrations in their dams resulted mainly from placental transfer rather than from the ingestion of colostrum.

[Pharmacodynamics of dihydroartemisinine against Plasmodium berghei in mice].

Dihydroartemisinine (DHA) is a derivative of qinghaosu(artemisine), which is an antimalarial drug. DHA was given to mice inoculated with Plasmodium berhgei ANKA strain by intramuscular injection. Within proper dose range, the dose-effect and time-effect curves of DHA against the malaria can be described by y = 4.9960 + 2.9536 x and y = 7.2654 - 0.3414 t, respectively, where y is the estimated value of parasitemia suppressing rate in probit; x is log dose; t is the period since drug administration im. Accordingly, the ED50 = 1.00 +/- 0.13 mg/kg and the time of half-effect was 6.6 h, when the dose was 5.0 mg/kg im. The elimination rate constant of the effective dose in vivo was 0.2662h-1 with a half-life of 2.6 h.