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1.
Mar Drugs ; 21(2)2023 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-36827097

RESUMEN

As one of the first families of marine natural products to undergo clinical trials, the didemnin depsipeptides have played a significant role in inspiring the discovery of marine drugs. Originally developed as anticancer therapeutics, the recent re-evaluation of these compounds including synthetically derived dehydrodidemnin B or Aplidine, has led to their advancement towards antiviral applications. While conventionally associated with production in colonial tunicates of the family Didemnidae, recent studies have identified their biosynthetic gene clusters from the marine-derived bacteria Tistrella mobilis. While these studies confirm the production of didemnin X/Y, the low titer and general lack of understanding of their biosynthesis in Tistrella currently prevents the development of effective microbial or synthetic biological approaches for their production. To this end, we conducted a survey of known species of Tistrella and report on their ability to produce the didemnin depsipeptides. These data were used to develop conditions to produce didemnin B at titers over 15 mg/L.


Asunto(s)
Antineoplásicos , Depsipéptidos , Antineoplásicos/química , Depsipéptidos/química , Péptidos Cíclicos/química
2.
J Am Chem Soc ; 142(9): 4114-4120, 2020 03 04.
Artículo en Inglés | MEDLINE | ID: mdl-32045230

RESUMEN

This report describes the first application of the novel NMR-based machine learning tool "Small Molecule Accurate Recognition Technology" (SMART 2.0) for mixture analysis and subsequent accelerated discovery and characterization of new natural products. The concept was applied to the extract of a filamentous marine cyanobacterium known to be a prolific producer of cytotoxic natural products. This environmental Symploca extract was roughly fractionated, and then prioritized and guided by cancer cell cytotoxicity, NMR-based SMART 2.0, and MS2-based molecular networking. This led to the isolation and rapid identification of a new chimeric swinholide-like macrolide, symplocolide A, as well as the annotation of swinholide A, samholides A-I, and several new derivatives. The planar structure of symplocolide A was confirmed to be a structural hybrid between swinholide A and luminaolide B by 1D/2D NMR and LC-MS2 analysis. A second example applies SMART 2.0 to the characterization of structurally novel cyclic peptides, and compares this approach to the recently appearing "atomic sort" method. This study exemplifies the revolutionary potential of combined traditional and deep learning-assisted analytical approaches to overcome longstanding challenges in natural products drug discovery.


Asunto(s)
Productos Biológicos/química , Aprendizaje Automático , Redes Neurales de la Computación , Productos Biológicos/aislamiento & purificación , Productos Biológicos/toxicidad , Línea Celular Tumoral , Quimioinformática , Cianobacterias/química , Humanos , Espectroscopía de Resonancia Magnética , Péptidos Cíclicos/química , Péptidos Cíclicos/aislamiento & purificación , Péptidos Cíclicos/toxicidad
3.
Molecules ; 25(2)2020 Jan 14.
Artículo en Inglés | MEDLINE | ID: mdl-31947506

RESUMEN

Natural product research continues to be a productive source of unusual chemistry, producing novel compounds for biomedical applications and, increasingly, sustainably providing commercially useful compounds [...].


Asunto(s)
Productos Biológicos/farmacología , Biotecnología/tendencias , Evaluación Preclínica de Medicamentos , Industria Farmacéutica/tendencias , Animales , Humanos
4.
Angew Chem Int Ed Engl ; 59(3): 1144-1148, 2020 01 13.
Artículo en Inglés | MEDLINE | ID: mdl-31696595

RESUMEN

The discovery of biologically active small molecules requires sifting through large amounts of data to identify unique or unusual arrangements of atoms. Here, we develop, test and evaluate an atom-based sort to identify novel features of secondary metabolites and demonstrate its use to evaluate novelty in marine microbial and sponge extracts. This study outlines an important ongoing advance towards the translation of autonomous systems to identify, and ultimately elucidate, atomic novelty within a complex mixture of small molecules.

5.
Nat Chem Biol ; 12(10): 773-5, 2016 10.
Artículo en Inglés | MEDLINE | ID: mdl-27547923

RESUMEN

Colibactin is an as-yet-uncharacterized genotoxic secondary metabolite produced by human gut bacteria. Here we report the biosynthetic discovery of two new precolibactin molecules from Escherichia coli, including precolibactin-886, which uniquely incorporates the highly sought genotoxicity-associated aminomalonate building block into its unprecedented macrocyclic structure. This work provides new insights into the biosynthetic logic and mode of action of this colorectal-cancer-linked microbial chemical.


Asunto(s)
Malonatos/metabolismo , Péptidos/metabolismo , Policétidos/metabolismo , Escherichia coli/metabolismo , Humanos , Malonatos/química , Conformación Molecular , Péptidos/química , Policétidos/química
6.
J Org Chem ; 83(22): 13670-13677, 2018 11 16.
Artículo en Inglés | MEDLINE | ID: mdl-30280573

RESUMEN

Three decahydroisoquinoline alkaloids, lepadins I-K, were isolated from a specimen of Didemnum sp. collected in the Bahamas. The structures of the new compounds were assigned by an integrated analysis of MS, IR, and 1H, 13C, and 2D NMR spectra. Like previously reported lepadins, the structures of the new compounds contain a decahydroquinoline heterocyclic core in lepadin I, and a new variation, an octahydroquinoline in lepadin J, but differ from earlier reported compounds by acylation of the 3-hydroxyl group by a rare 3'-methylthioacrylate. The absolute configuration of lepadin I was solved by interpretation of NOE measurements, and exciton coupled circular dichroism (ECCD) of the corresponding N- p-bromobenzoyl derivative. The latter constitutes a general method for determination of absolute configuration of the entire lepadin family. The configuration of the remote side-chain secondary carbinol was solved by the modified Mosher's esters method. Lepadin I inhibited butyrylcholineesterase (BuChE, IC50 3.1 µM), but only weakly inhibited acetylcholineesterase (AChE) (10% at 100 µM).


Asunto(s)
Alcaloides/química , Isoquinolinas/química , Quinolinas/química , Urocordados/química , Animales , Bahamas , Modelos Moleculares , Estructura Molecular
7.
Molecules ; 23(6)2018 06 18.
Artículo en Inglés | MEDLINE | ID: mdl-29912151

RESUMEN

Viruses are underrepresented as targets in pharmacological screening efforts, given the difficulties of devising suitable cell-based and biochemical assays. In this study we found that a pre-fractionated organic extract of the Red Sea sponge Amphimedon chloros was able to inhibit the West Nile Virus NS3 protease (WNV NS3). Using liquid chromatography⁻mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectroscopy, the identity of the bioactive compound was determined as a 3-alkylpyridinium with m/z = 190.16. Diffusion Ordered Spectroscopy (DOSY) NMR and NMR relaxation rate analysis suggest that the bioactive compound forms oligomers of up to 35 kDa. We observed that at 9.4 µg/mL there was up to 40⁻70% inhibitory activity on WNV NS3 protease in orthogonal biochemical assays for solid phase extracts (SPE) of A. chloros. However, the LC-MS purified fragment was effective at inhibiting the protease up to 95% at an approximate amount of 2 µg/mL with negligible cytotoxicity to HeLa cells based on a High-Content Screening (HCS) cytological profiling strategy. To date, 3-alkylpyridinium type natural products have not been reported to show antiviral activity since the first characterization of halitoxin, or 3-alkylpyridinium, in 1978. This study provides the first account of a 3-alkylpyridinium complex that exhibits a proposed antiviral activity by inhibiting the NS3 protease. We suggest that the here-described compound can be further modified to increase its stability and tested in a cell-based assay to explore its full potential as a potential novel antiviral capable of inhibiting WNV replication.


Asunto(s)
Antivirales/aislamiento & purificación , Poríferos/química , Inhibidores de Proteasas/aislamiento & purificación , Compuestos de Piridinio/aislamiento & purificación , Proteínas no Estructurales Virales/antagonistas & inhibidores , Virus del Nilo Occidental/enzimología , Animales , Antivirales/química , Antivirales/farmacología , Cromatografía de Gases y Espectrometría de Masas , Células HeLa , Humanos , Espectroscopía de Resonancia Magnética , Modelos Moleculares , Estructura Molecular , Inhibidores de Proteasas/química , Inhibidores de Proteasas/farmacología , Compuestos de Piridinio/química , Compuestos de Piridinio/farmacología , Relación Estructura-Actividad , Virus del Nilo Occidental/efectos de los fármacos
8.
Genet Sel Evol ; 49(1): 42, 2017 05 02.
Artículo en Inglés | MEDLINE | ID: mdl-28464791

RESUMEN

BACKGROUND: Genetic progress in selection for greater body mass and meat yield in poultry has been associated with an increase in gait problems which are detrimental to productivity and welfare. The incidence of suboptimal gait in breeding flocks is controlled through the use of a visual gait score, which is a subjective assessment of walking ability of each bird. The subjective nature of the visual gait score has led to concerns over its effectiveness in reducing the incidence of suboptimal gait in poultry through breeding. The aims of this study were to assess the reliability of the current visual gait scoring system in ducks and to develop a more objective method to select for better gait. RESULTS: Experienced gait scorers assessed short video clips of walking ducks to estimate the reliability of the current visual gait scoring system. Kendall's coefficients of concordance between and within observers were estimated at 0.49 and 0.75, respectively. In order to develop a more objective scoring system, gait components were visually scored on more than 4000 pedigreed Pekin ducks and genetic parameters were estimated for these components. Gait components, which are a more objective measure, had heritabilities that were as good as, or better than, those of the overall visual gait score. CONCLUSIONS: Measurement of gait components is simpler and therefore more objective than the standard visual gait score. The recording of gait components can potentially be automated, which may increase accuracy further and may improve heritability estimates. Genetic correlations were generally low, which suggests that it is possible to use gait components to select for an overall improvement in both economic traits and gait as part of a balanced breeding programme.


Asunto(s)
Patos/genética , Carácter Cuantitativo Heredable , Velocidad al Caminar/genética , Animales , Fenotipo , Selección Artificial
9.
Magn Reson Chem ; 55(4): 263-268, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26951950

RESUMEN

Ultra-high resolution band-selective HSQC (bsHSQC) has been employed for detection of 35 Cl-37 Cl isotope shifted 13 C NMR signals for assignment of regioisomerism in bromo-chloro natural products. Optimum pulse sequence and instrumental parameters for maximization of detection of the isotope shifts were explored. The chlorine isotope shifts (Δδ) were detected within crosspeaks and were shown to vary with hybridization of 13 C, substitution of 13 C, presence of ß-chloro substituents, and their relative configuration. Deconvolution of Cl-substituted CH bsHSQC crosspeaks may provide other useful information, including a potentially MS-independent method for quantitating 37 Cl/35 C isotopic fractionation during the biosynthesis of halogenated natural products. Copyright © 2016 John Wiley & Sons, Ltd.


Asunto(s)
Productos Biológicos/química , Cloro/química , Espectroscopía de Resonancia Magnética/métodos , Isótopos de Carbono , Fraccionamiento Químico , Cromatografía Líquida de Alta Presión/métodos , Humanos , Isótopos/química , Estructura Molecular
10.
Proc Natl Acad Sci U S A ; 110(47): E4407-16, 2013 Nov 19.
Artículo en Inglés | MEDLINE | ID: mdl-24191063

RESUMEN

Glycosyl groups are an essential mediator of molecular interactions in cells and on cellular surfaces. There are very few methods that directly relate sugar-containing molecules to their biosynthetic machineries. Here, we introduce glycogenomics as an experiment-guided genome-mining approach for fast characterization of glycosylated natural products (GNPs) and their biosynthetic pathways from genome-sequenced microbes by targeting glycosyl groups in microbial metabolomes. Microbial GNPs consist of aglycone and glycosyl structure groups in which the sugar unit(s) are often critical for the GNP's bioactivity, e.g., by promoting binding to a target biomolecule. GNPs are a structurally diverse class of molecules with important pharmaceutical and agrochemical applications. Herein, O- and N-glycosyl groups are characterized in their sugar monomers by tandem mass spectrometry (MS) and matched to corresponding glycosylation genes in secondary metabolic pathways by a MS-glycogenetic code. The associated aglycone biosynthetic genes of the GNP genotype then classify the natural product to further guide structure elucidation. We highlight the glycogenomic strategy by the characterization of several bioactive glycosylated molecules and their gene clusters, including the anticancer agent cinerubin B from Streptomyces sp. SPB74 and an antibiotic, arenimycin B, from Salinispora arenicola CNB-527.


Asunto(s)
Vías Biosintéticas/genética , Minería de Datos/métodos , Genes Microbianos/genética , Genómica/métodos , Metaboloma , Espectrometría de Masas en Tándem/métodos , Productos Biológicos/química , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Glicosilación , Espectroscopía de Resonancia Magnética , Estructura Molecular
11.
Proc Natl Acad Sci U S A ; 110(28): E2611-20, 2013 Jul 09.
Artículo en Inglés | MEDLINE | ID: mdl-23798442

RESUMEN

The ability to correlate the production of specialized metabolites to the genetic capacity of the organism that produces such molecules has become an invaluable tool in aiding the discovery of biotechnologically applicable molecules. Here, we accomplish this task by matching molecular families with gene cluster families, making these correlations to 60 microbes at one time instead of connecting one molecule to one organism at a time, such as how it is traditionally done. We can correlate these families through the use of nanospray desorption electrospray ionization MS/MS, an ambient pressure MS technique, in conjunction with MS/MS networking and peptidogenomics. We matched the molecular families of peptide natural products produced by 42 bacilli and 18 pseudomonads through the generation of amino acid sequence tags from MS/MS data of specific clusters found in the MS/MS network. These sequence tags were then linked to biosynthetic gene clusters in publicly accessible genomes, providing us with the ability to link particular molecules with the genes that produced them. As an example of its use, this approach was applied to two unsequenced Pseudoalteromonas species, leading to the discovery of the gene cluster for a molecular family, the bromoalterochromides, in the previously sequenced strain P. piscicida JCM 20779(T). The approach itself is not limited to 60 related strains, because spectral networking can be readily adopted to look at molecular family-gene cluster families of hundreds or more diverse organisms in one single MS/MS network.


Asunto(s)
Familia de Multigenes , Espectrometría de Masas en Tándem/métodos , Secuencia de Aminoácidos , Bacillus/genética , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Datos de Secuencia Molecular , Péptidos/química , Péptidos/genética , Pseudomonas/genética
12.
J Am Chem Soc ; 137(38): 12343-51, 2015 Sep 30.
Artículo en Inglés | MEDLINE | ID: mdl-26336030

RESUMEN

Four new chlorobromohydrins, mollenynes B-E, were isolated from the marine sponge Spirastrella mollis collected from Hogsty Reef, Bahamas. Their structures were elucidated by integrated analysis of NMR, MS, and computational methods. A high-resolution band-selective HSQC experiment was developed to identify (13)C NMR signals in samples at the nanomole-scale that arise from Cl-substituted (13)C by exploiting the (35)Cl/(37)Cl isotope shift.


Asunto(s)
Productos Biológicos/química , Productos Biológicos/aislamiento & purificación , Clorhidrinas/química , Clorhidrinas/aislamiento & purificación , Poríferos/química , Teoría Cuántica , Animales , Conformación Molecular , Estereoisomerismo
13.
Genet Sel Evol ; 47: 88, 2015 Nov 17.
Artículo en Inglés | MEDLINE | ID: mdl-26576729

RESUMEN

BACKGROUND: Poultry account for the most numerous species farmed for meat and have been subject to intense selection over approximately 60 generations. To assess morphological changes which have occurred in the avian leg due to selection for rapid growth and high meat yields, divergent lines of chicken (Gallus gallus) and duck (Anas platyrhynchos) were studied between 3 and 7 weeks of age. For each line, femoral and tibiotarsal morphology was recorded using computed tomography scanning and tibiotarsal bone quality measures (stiffness, bending stress and porosity) were assessed. RESULTS: In chicken and duck, divergence in hindlimb morphology has occurred in the commercial meat lines compared to their lighter conspecifics. As expected, the differences were largest between species. Leg development nears completion much earlier in ducks than in chickens. Duck tibiotarsi showed a large degree of lateral curvature, which is expected to affect foot position during swimming and walking, and thus to influence gait. All lines have adapted their tibiotarsal morphology to suit the loading forces they experience; however bone quality was found to be poorer in chickens. CONCLUSIONS: We demonstrate that intensive selection for growth rate in both chickens and ducks has resulted in leg morphology changes, which are likely to influence gait. Ducks represent an interesting compromise of adaptation for efficient locomotion in two media-on land and in water. Some aspects of bone morphology in the duck, such as lateral curvature of the tibiotarsus, may result from adaptation to swimming, which potentially imposes limitations on terrestrial locomotion.


Asunto(s)
Pollos , Patos , Evolución Molecular , Estudios de Asociación Genética , Miembro Posterior/anatomía & histología , Selección Genética , Animales , Femenino , Masculino , Fenotipo , Carácter Cuantitativo Heredable
14.
J Nat Prod ; 78(7): 1671-82, 2015 Jul 24.
Artículo en Inglés | MEDLINE | ID: mdl-26149623

RESUMEN

An innovative approach was developed for the discovery of new natural products by combining mass spectrometric metabolic profiling with genomic analysis and resulted in the discovery of the columbamides, a new class of di- and trichlorinated acyl amides with cannabinomimetic activity. Three species of cultured marine cyanobacteria, Moorea producens 3L, Moorea producens JHB, and Moorea bouillonii PNG, were subjected to genome sequencing and analysis for their recognizable biosynthetic pathways, and this information was then compared with their respective metabolomes as detected by MS profiling. By genome analysis, a presumed regulatory domain was identified upstream of several previously described biosynthetic gene clusters in two of these cyanobacteria, M. producens 3L and M. producens JHB. A similar regulatory domain was identified in the M. bouillonii PNG genome, and a corresponding downstream biosynthetic gene cluster was located and carefully analyzed. Subsequently, MS-based molecular networking identified a series of candidate products, and these were isolated and their structures rigorously established. On the basis of their distinctive acyl amide structure, the most prevalent metabolite was evaluated for cannabinomimetic properties and found to be moderate affinity ligands for CB1.


Asunto(s)
Productos Biológicos/química , Productos Biológicos/aislamiento & purificación , Cianobacterias/química , Productos Biológicos/farmacología , Vías Biosintéticas/genética , Cianobacterias/genética , Genómica , Metaboloma , Metabolómica , Estructura Molecular , Familia de Multigenes , Resonancia Magnética Nuclear Biomolecular , Receptor Cannabinoide CB1/metabolismo
15.
Proc Natl Acad Sci U S A ; 109(34): 13811-6, 2012 Aug 21.
Artículo en Inglés | MEDLINE | ID: mdl-22869730

RESUMEN

In polymicrobial infections, microbes can interact with both the host immune system and one another through direct contact or the secretion of metabolites, affecting disease progression and treatment options. The thick mucus in the lungs of patients with cystic fibrosis is highly susceptible to polymicrobial infections by opportunistic pathogens, including the bacterium Pseudomonas aeruginosa and the fungus Aspergillus fumigatus. Unravelling the hidden molecular interactions within such polymicrobial communities and their metabolic exchange processes will require effective enabling technologies applied to model systems. In the present study, MALDI-TOF and MALDI-FT-ICR imaging mass spectrometry (MALDI-IMS) combined with MS/MS networking were used to provide insight into the interkingdom interaction between P. aeruginosa and A. fumigatus at the molecular level. The combination of these technologies enabled the visualization and identification of metabolites secreted by these microorganisms grown on agar. A complex molecular interplay was revealed involving suppression, increased production, and biotransformation of a range of metabolites. Of particular interest is the observation that P. aeruginosa phenazine metabolites were converted by A. fumigatus into other chemical entities with alternative properties, including enhanced toxicities and the ability to induce fungal siderophores. This work highlights the capabilities of MALDI-IMS and MS/MS network analysis to study interkingdom interactions and provides insight into the complex nature of polymicrobial metabolic exchange and biotransformations.


Asunto(s)
Espectrometría de Masas/métodos , Pseudomonas aeruginosa/metabolismo , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Aspergillus fumigatus/metabolismo , Cromatografía Líquida de Alta Presión/métodos , Coinfección/microbiología , Fibrosis Quística/microbiología , Humanos , Iones , Técnicas Microbiológicas , Modelos Biológicos , Pseudomonas aeruginosa/genética , Especificidad de la Especie , Espectrometría de Masas en Tándem/métodos , Factores de Tiempo
17.
J Nat Prod ; 77(11): 2553-60, 2014 Nov 26.
Artículo en Inglés | MEDLINE | ID: mdl-25343669

RESUMEN

A small library of synthetic (-)-palmyrolide A diastereomers, analogues, and acyclic precursors have been examined with respect to their interaction with voltage-gated sodium channels (VGSCs). Toward this goal, the ability of (-)-palmyrolide A and analogues to antagonize veratridine-stimulated Na(+) influx in primary cultures of mouse cerebrocortical neurons was assessed. We found that synthetic (-)-palmyrolide A and its enantiomer functioned as VGSC antagonists to block veratridine-induced sodium influx. A detailed NMR and computational analysis of four diastereomers revealed that none had the same combination of shape and electrostatic potential as exhibited by natural (-)-palmyrolide A. These data indicate that the relative configuration about the tert-butyl and methyl substituents appears to be a prerequisite for biological function. Additional testing revealed that the enamide double bond was not necessary for blocking veratridine-induced sodium influx, whereas the acyclic analogues and other macrolide diastereomers tested were inactive as inhibitors of VGSCs, suggesting that the intact macrolide was required.


Asunto(s)
Macrólidos/química , Macrólidos/farmacología , Bloqueadores del Canal de Sodio Activado por Voltaje/farmacología , Animales , Ratones , Estructura Molecular , Neuronas/efectos de los fármacos , Estereoisomerismo , Veratridina/farmacología , Bloqueadores del Canal de Sodio Activado por Voltaje/química
18.
Biochem J ; 450(1): 231-42, 2013 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-23216060

RESUMEN

In the present paper we show a comprehensive in vitro, ex vivo and in vivo study on hydrolytic detoxification of nerve agent and pesticide OPs (organophosphates) catalysed by purified hBChE (human butyrylcholinesterase) in combination with novel non-pyridinium oxime reactivators. We identified TAB2OH (2-trimethylammonio-6-hydroxybenzaldehyde oxime) as an efficient reactivator of OP-hBChE conjugates formed by the nerve agents VX and cyclosarin, and the pesticide paraoxon. It was also functional in reactivation of sarin- and tabun-inhibited hBChE. A 3-5-fold enhancement of in vitro reactivation of VX-, cyclosarin- and paraoxon-inhibited hBChE was observed when compared with the commonly used N-methylpyridinium aldoxime reactivator, 2PAM (2-pyridinealdoxime methiodide). Kinetic analysis showed that the enhancement resulted from improved molecular recognition of corresponding OP-hBChE conjugates by TAB2OH. The unique features of TAB2OH stem from an exocyclic quaternary nitrogen and a hydroxy group, both ortho to an oxime group on a benzene ring. pH-dependences reveal participation of the hydroxy group (pKa=7.6) forming an additional ionizing nucleophile to potentiate the oxime (pKa=10) at physiological pH. The TAB2OH protective indices in therapy of sarin- and paraoxon-exposed mice were enhanced by 30-60% when they were treated with a combination of TAB2OH and sub-stoichiometric hBChE. The results of the present study establish that oxime-assisted catalysis is feasible for OP bioscavenging.


Asunto(s)
Butirilcolinesterasa/metabolismo , Sustancias para la Guerra Química/metabolismo , Organofosfatos/metabolismo , Oximas/química , Paraoxon/metabolismo , Sarín/metabolismo , Animales , Catálisis , Sustancias para la Guerra Química/toxicidad , Femenino , Humanos , Concentración de Iones de Hidrógeno , Inactivación Metabólica , Cinética , Ratones , Ratones Endogámicos , Organofosfatos/toxicidad , Oximas/metabolismo , Paraoxon/toxicidad , Sarín/toxicidad
19.
Biomolecules ; 14(4)2024 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-38672438

RESUMEN

Abnormal blood coagulation is a major health problem and natural anticoagulants from blood-feeding organisms have been investigated as novel therapeutics. NAPc2, a potent nematode-derived inhibitor of coagulation, has an unusual mode of action that requires coagulation factor Xa but does not inhibit it. Molecular dynamics simulations of NAPc2 and factor Xa were generated to better understand NAPc2. The simulations suggest that parts of NAPc2 become more rigid upon binding factor Xa and reveal that two highly conserved residues form an internal salt bridge that stabilises the bound conformation. Clotting time assays with mutants confirmed the utility of the salt bridge and suggested that it is a conserved mechanism for stabilising the bound conformation of secondary structure-poor protease inhibitors.


Asunto(s)
Anticoagulantes , Factor Xa , Simulación de Dinámica Molecular , Unión Proteica , Animales , Anticoagulantes/química , Anticoagulantes/farmacología , Factor Xa/metabolismo , Factor Xa/química , Nematodos/metabolismo , Nematodos/efectos de los fármacos , Humanos , Coagulación Sanguínea/efectos de los fármacos , Proteínas del Helminto/química , Proteínas del Helminto/metabolismo , Proteínas del Helminto/genética , Sitios de Unión
20.
Biophys J ; 105(4): 1004-17, 2013 Aug 20.
Artículo en Inglés | MEDLINE | ID: mdl-23972852

RESUMEN

Arginine-rich motifs (ARMs) capable of binding diverse RNA structures play critical roles in transcription, translation, RNA trafficking, and RNA packaging. The regulatory HIV-1 protein Rev is essential for viral replication and belongs to the ARM family of RNA-binding proteins. During the early stages of the HIV-1 life cycle, incompletely spliced and full-length viral mRNAs are very inefficiently recognized by the splicing machinery of the host cell and are subject to degradation in the cell nucleus. These transcripts harbor the Rev Response Element (RRE), which orchestrates the interaction with the Rev ARM and the successive Rev-dependent mRNA export pathway. Based on established criteria for predicting intrinsic disorder, such as hydropathy, combined with significant net charge, the very basic primary sequences of ARMs are expected to adopt coil-like structures. Thus, we initiated this study to investigate the conformational changes of the Rev ARM associated with RNA binding. We used multidimensional NMR and circular dichroism spectroscopy to monitor the observed structural transitions, and described the conformational landscapes using statistical ensemble and molecular-dynamics simulations. The combined spectroscopic and simulated results imply that the Rev ARM is intrinsically disordered not only as an isolated peptide but also when it is embedded into an oligomerization-deficient Rev mutant. RRE recognition triggers a crucial coil-to-helix transition employing an induced-fit mechanism.


Asunto(s)
Arginina/metabolismo , Pliegue de Proteína , ARN/metabolismo , Elementos de Respuesta , Productos del Gen rev del Virus de la Inmunodeficiencia Humana/química , Productos del Gen rev del Virus de la Inmunodeficiencia Humana/metabolismo , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Proteínas Intrínsecamente Desordenadas/química , Proteínas Intrínsecamente Desordenadas/genética , Proteínas Intrínsecamente Desordenadas/metabolismo , Simulación de Dinámica Molecular , Datos de Secuencia Molecular , Mutación , Unión Proteica , Productos del Gen rev del Virus de la Inmunodeficiencia Humana/genética
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