RESUMEN
In the 19th century, there were several major cholera pandemics in the Indian subcontinent, Europe, and North America. The causes of these outbreaks and the genomic strain identities remain a mystery. We used targeted high-throughput sequencing to reconstruct the Vibrio cholerae genome from the preserved intestine of a victim of the 1849 cholera outbreak in Philadelphia, part of the second cholera pandemic. This O1 biotype strain has 95 to 97% similarity with the classical O395 genome, differing by 203 single-nucleotide polymorphisms (SNPs), lacking three genomic islands, and probably having one or more tandem cholera toxin prophage (CTX) arrays, which potentially affected its virulence. This result highlights archived medical remains as a potential resource for investigations into the genomic origins of past pandemics.
Asunto(s)
Cólera/historia , Pandemias/historia , Vibrio cholerae/genética , Técnicas de Tipificación Bacteriana , Cólera/epidemiología , Cólera/microbiología , ADN Bacteriano/aislamiento & purificación , ADN Mitocondrial/análisis , Evolución Molecular , Genoma Bacteriano , Islas Genómicas , Historia del Siglo XIX , Humanos , Intestinos/microbiología , Intestinos/patología , Masculino , Philadelphia/epidemiología , Filogenia , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN , Vibrio cholerae/clasificación , Vibrio cholerae/patogenicidad , Virulencia , Factores de Virulencia/análisisRESUMEN
We report metrics from complete genome capture of nuclear DNA from extinct mammoths using biotinylated RNAs transcribed from an Asian elephant DNA extract. Enrichment of the nuclear genome ranged from 1.06- to 18.65-fold, to an apparent maximum threshold of â¼80% on-target. This projects an order of magnitude less costly complete genome sequencing from long-dead organisms, even when a reference genome is unavailable for bait design.
Asunto(s)
Genoma , Genómica/métodos , Mamuts/genética , Análisis de Secuencia de ADN/métodos , Animales , ADN/genética , ADN/aislamiento & purificación , Elefantes/genética , Fósiles , Historia Antigua , Alineación de Secuencia/métodosRESUMEN
Pregnancy complications are poorly represented in the archeological record, despite their importance in contemporary and ancient societies. While excavating a Byzantine cemetery in Troy, we discovered calcified abscesses among a woman's remains. Scanning electron microscopy of the tissue revealed 'ghost cells', resulting from dystrophic calcification, which preserved ancient maternal, fetal and bacterial DNA of a severe infection, likely chorioamnionitis. Gardnerella vaginalis and Staphylococcus saprophyticus dominated the abscesses. Phylogenomic analyses of ancient, historical, and contemporary data showed that G. vaginalis Troy fell within contemporary genetic diversity, whereas S. saprophyticus Troy belongs to a lineage that does not appear to be commonly associated with human disease today. We speculate that the ecology of S. saprophyticus infection may have differed in the ancient world as a result of close contacts between humans and domesticated animals. These results highlight the complex and dynamic interactions with our microbial milieu that underlie severe maternal infections.
Asunto(s)
Absceso/patología , Fósiles , Infecciones por Bacterias Grampositivas/patología , Complicaciones Infecciosas del Embarazo/patología , Absceso/microbiología , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Femenino , Gardnerella vaginalis/clasificación , Gardnerella vaginalis/genética , Infecciones por Bacterias Grampositivas/microbiología , Humanos , Microscopía Electrónica de Rastreo , Embarazo , Staphylococcus saprophyticus/clasificación , Staphylococcus saprophyticus/genéticaRESUMEN
Ancient human remains of paleopathological interest typically contain highly degraded DNA in which pathogenic taxa are often minority components, making sequence-based metagenomic characterization costly. Microarrays may hold a potential solution to these challenges, offering a rapid, affordable, and highly informative snapshot of microbial diversity in complex samples without the lengthy analysis and/or high cost associated with high-throughput sequencing. Their versatility is well established for modern clinical specimens, but they have yet to be applied to ancient remains. Here we report bacterial profiles of archaeological and historical human remains using the Lawrence Livermore Microbial Detection Array (LLMDA). The array successfully identified previously-verified bacterial human pathogens, including Vibrio cholerae (cholera) in a 19th century intestinal specimen and Yersinia pestis ("Black Death" plague) in a medieval tooth, which represented only minute fractions (0.03% and 0.08% alignable high-throughput shotgun sequencing reads) of their respective DNA content. This demonstrates that the LLMDA can identify primary and/or co-infecting bacterial pathogens in ancient samples, thereby serving as a rapid and inexpensive paleopathological screening tool to study health across both space and time.