RESUMEN
Hepatocyte growth factor (HGF) promotes regeneration of the central nervous system, but its effects on the peripheral nervous system remain unclear. This study was conducted to elucidate the effect of HGF on regeneration of the murine facial nerve after crush injury. To do so, a replication-defective herpes simplex virus vector that incorporated HGF was prepared (HSV-HGF). The main trunk of the facial nerve was compressed by mosquito hemostats, and HSV-HGF, control vector or medium was then applied to the compressed nerve. We found that mice in the HGF group required significantly fewer days for complete recovery from nerve compression. Furthermore, the amplitude of the evoked buccinator muscle compound action potential increased following HSV-HGF application. HGF expression in and around the compressed nerve was demonstrated by enzyme-linked immunoassay and immunohistochemistry. In addition, HSV-HGF introduction around the damaged nerve significantly accelerated recovery of function of the facial nerve. These data suggest a possible role of HGF in promoting facial nerve regeneration after nerve damage. Furthermore, this viral delivery method may be applied clinically for many types of severe facial palsy during facial nerve decompression surgery.
Asunto(s)
Traumatismos del Nervio Facial/terapia , Terapia Genética/métodos , Factor de Crecimiento de Hepatocito/genética , Regeneración Nerviosa/efectos de los fármacos , Simplexvirus/genética , Animales , Nervio Facial/fisiología , Vectores Genéticos , Ratones , Síndromes de Compresión Nerviosa/terapia , Regeneración Nerviosa/genéticaRESUMEN
Progress in imaging techniques and nano-manipulation of single molecules has been remarkable. These techniques have allowed the accurate determination of myosin-head-induced displacements and of how the mechanical cycles of the actomyosin motor are coupled to ATP hydrolysis. This has been achieved by measuring mechanical and chemical events of actomyosin directly at the single molecule level. Recent studies have made detailed measurements of myosin step size and mechanochemical coupling. The results of these studies suggest a new model for the mechanism of motion underlying actomyosin motors, which differs from the currently accepted lever-arm swinging model.
Asunto(s)
Actomiosina/química , Actomiosina/metabolismo , Proteínas Motoras Moleculares/química , Proteínas Motoras Moleculares/metabolismo , Animales , Modelos Químicos , Miosinas/química , Miosinas/metabolismo , Conformación ProteicaRESUMEN
Immunosuppression by anti-adhesion molecule antibody of free or vascularized skin allograft rejection was investigated in rats. Lewis (LEW, RT11) rats were used as donors and Fisher (F344, RT11v1) rats as the recipients. When F344 rats were treated intraperitoneally (i.p.) with anti-intercellular adhesion molecule-1 (ICAM-1) mAb (1A29) (3 mg/kg/day) and anti-leukocyte function-associated antigen-1 (LFA-1) mAb (WT.1) (3 mg/kg/day) one day prior to grafting and daily after grafting for nine days, free skin graft survival was prolonged only slightly compared with that in control rats which were injected i.p. with a daily dose of 6 mg/kg of anti-TNP mAbs (H1-6-2) one day prior to grafting and daily after grafting for nine days. (Mean survival time [MST] of the free skin graft was 11.2 +/- 0.6 days in the control group and 13.4 +/- 0.3 days in the 1A29 + WT-1 treated group [p < 0.01], respectively.) On the other hand, the vascularized graft survival was prolonged significantly in anti-ICAM-1/LFA-1 mAbs-treated F344 rats as compared with that in control rats. (The mean vascularized graft survival time was 14.2 +/- 0.7 days in the control group and 21.5 +/- 1.9 days in 1A29 + WT-1 treated group [p < 0.002]). Our results suggest that interaction with ICAM-1 and LFA-1 is more important in the rejection of vascularized skin allografts than that of free skin allografts.
Asunto(s)
Anticuerpos Monoclonales/farmacología , Rechazo de Injerto/prevención & control , Inmunosupresores/farmacología , Molécula 1 de Adhesión Intercelular/inmunología , Antígeno-1 Asociado a Función de Linfocito/inmunología , Trasplante de Piel/inmunología , Animales , Endotelio Vascular/inmunología , Supervivencia de Injerto/inmunología , Masculino , Ratas , Ratas Endogámicas F344 , Ratas Endogámicas Lew , Trasplante HomólogoRESUMEN
The suppression of the temporal bone's pneumatized cavities is commonly observed in chronic middle ear inflammatory disease, but this fact has not been explained in respect to its etiology, i.e., whether the poorly pneumatized cavity induces chronic middle ear infection or middle ear inflammatory condition suppresses the pneumatization of cellulae. We studied the effect of middle ear infection and eustachian tubal dysfunction in the early stage of life upon the development of pneumatization of the mastoid using 13 pigs, whose mastoid is comparable to that of man. We used two methods, the eustachian tube stenosis test and the tympanic membrane paraffin infusion test. The present study indicated that in six pigs infection of the middle ear in the early stage of life suppressed the normal development of pneumatization of the mastoid bone in pigs.
Asunto(s)
Apófisis Mastoides/crecimiento & desarrollo , Otitis Media/fisiopatología , Animales , Trompa Auditiva/crecimiento & desarrollo , Trompa Auditiva/patología , Apófisis Mastoides/patología , Modelos Biológicos , Otitis Media/patología , Porcinos , Membrana Timpánica/crecimiento & desarrollo , Membrana Timpánica/patologíaRESUMEN
The distally based superficial sural artery flap, first described as a distally based neuroskin flap by Masquelet et al., is a skin island flap supplied by the vascular axis of the sural nerve. The aim of this paper is to refine the surgical technique in order to establish the reliability of this flap. We treated 20 patients with 21 distally based superficial sural artery flaps, including 2 fascial flaps. All flaps survived. Most flaps showed slight venous congestion. In the largest flap (10 cm wide by 13 cm long), edema lasted 2 months. In the flap whose pedicle was raised without the deep fascia, there was necrosis at the distal tip of the flap. The advantages are the following: The blood supply is reliable, elevation is easy and quick, and major arteries are not sacrificed. This new flap may be useful in selected circumstances.
Asunto(s)
Traumatismos de la Pierna/cirugía , Pierna/irrigación sanguínea , Colgajos Quirúrgicos/métodos , Accidentes de Tránsito , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Supervivencia de Injerto , Humanos , Masculino , Persona de Mediana Edad , Reproducibilidad de los Resultados , Piel/irrigación sanguínea , Traumatismos de la Médula Espinal/complicaciones , Nervio SuralRESUMEN
BACKGROUND/PURPOSE: The aim of this study was to evaluate the advantages or disadvantages of laparoscopic pyloromyotomy compared with open transumbilical fold pyloromyotomy. METHODS: Thirty consecutive laparoscopic extramucosal pyloromyotomies (LP) performed from 1994 to 1997 were compared with 30 consecutive open pyloromyotomies (OP) performed during the same period with regard to age at operation, body weight, thickness of hypertrophied pyloric muscle, operating time, time of return to full feeding, frequency of postoperative emesis, surgical complications, and degree of surgical stress reflected by interleukin-6 (IL-6). LP was performed according to conventional techniques, and OP was performed using a transumbilical fold approach. RESULTS: The groups were matched for age at operation, preoperative clinical and physical status, laboratory data, and size of the hypertrophied pylorus assessed by ultrasonography. There was a learning curve with LP; the average operating time required for the first 10 cases was significantly longer than the time required for OP, but later cases took just as long as OP cases. Time taken to full feeding was significantly shorter in the LP group than the OP group (LP, 38 hours v OP, 64 hours). One case was converted from LP to OP because of mucosal perforation. The incidence of postoperative emesis was significantly higher in the OP group than in the LP group (OP, 25% v LP, 3%). The mean length of hospitalization was significantly shorter in LP (P < .01). The intraoperative peak values of IL-6 in LP were significantly lower than those in the OP group (P < .01). CONCLUSIONS: The advantages of LP are improved cosmesis, decreased surgical stress with earlier postoperative recovery, and shorter hospitalization. Because LP uses reusable devices, and the mean period of hospitalization is shorter, average operating costs could be reduced, representing a net saving in total hospital charges.
Asunto(s)
Laparoscopía/métodos , Estenosis Pilórica/cirugía , Ingestión de Alimentos , Humanos , Hipertrofia/cirugía , Lactante , Recién Nacido , Laparoscopios , Complicaciones Posoperatorias , Factores de Tiempo , Resultado del TratamientoRESUMEN
The intention of this experiment was to investigate whether anatomical variations of the temporal bone such as low middle fossa dura, anterior position of the sigmoid sinus or small mastoid process, which are often seen at surgery in cholesteatoma patients, are caused by inflammation in early childhood. In 7 pigs, 1.5 ml paraffin liquid were instilled into the left tympanic cavity 2 or 7 days after birth in order to produce inflammation of the middle ear and tubal dysfunction. After six and a half months the length and area of the mastoid process were significantly smaller on the left instilled side than on the right, normal side. In all cases there was remarkable hypocellularity and increased thickness of the cortical bone strongly supporting the environmental theory of pneumatization that inflammation suppresses the pneumatization process and growth of the mastoid process.
Asunto(s)
Apófisis Mastoides/crecimiento & desarrollo , Otitis Media con Derrame/fisiopatología , Animales , Apófisis Mastoides/anatomía & histología , Apófisis Mastoides/patología , Otitis Media con Derrame/patología , PorcinosRESUMEN
Involvement of bacteria in serous otitis media was studied in man and by animal experimentation (using rabbits). Bacteria from the middle ear fluid, the skin of the external ear canal and the epipharynx of 100 human ears were cultured. Bacteria were also cultured from 50 ears of rabbits with serous otitis media induced experimentally by tubal stenosis, in the same way as those taken from human ears. Bacteria were detected in the middle ear fluid of 61 human ears (61%), and in 48 human ears (48%) except for those with contamination. The bacteria detected in the middle ear fluid were the same as those detected in the epipharynx in 40 of the 50 rabbit ears during the period from the start of treatment to the 30th day. In ears which were treated for more than 30 days, the bacteria detected in the middle ear fluid tended not to be the same as those detected in the epipharynx. From these results it was inferred that inflammation of the epipharynx spread over the middle ear cavity through the auditory tube.
Asunto(s)
Otitis Media con Derrame/microbiología , Adolescente , Adulto , Anciano , Animales , Niño , Preescolar , Humanos , Persona de Mediana Edad , ConejosRESUMEN
The pharmacokinetics of cisplatin (cis-dichlorodiammineplatinum (II), CDDP) have been studied in 11 patients. Plasma and urine CDDP concentrations were determined by flameless atomic absorption spectrophotometry. The treatment schedule was administered either by a 3-hour and 4-hour i.v. infusion or a 1.5-hour i.a. infusion. In the 3-hour i.v. infusion, the peak level of plasma total CDDP was 4.09 micrograms/ml after the end of infusion and non-protein-bound CDDP was 0.78 microgram/ml. Plasma total CDDP level declined with two peaks, the half-life of the alpha phase being 3.3 hours and that of the beta phase being 5.29 days. The half-life of non-protein-bound CDDP was 1.6 hours. CDDP might have remained for a long time, because CDDP values were obtained 3 weeks after infusion and urinary recovery of CDDP showed low values. Its urinary recovery was 40.3% of the dose infusion in the first 24 hours and 48.0% in 96 hours. The protein-binding of CDDP was inhibited by sodium thiosulfate(STS) and renal urinary excretion was enhanced. Our data suggest that STS can be applied as a neutralizer of CDDP.
Asunto(s)
Cisplatino/metabolismo , Tiosulfatos/farmacología , Cisplatino/administración & dosificación , Esquema de Medicación , Neoplasias de Cabeza y Cuello/tratamiento farmacológico , Neoplasias de Cabeza y Cuello/metabolismo , Humanos , Infusiones Parenterales , Cinética , Espectrofotometría AtómicaRESUMEN
It is generally believed that a cause and effect relationship exists between chronic middle ear inflammatory conditions and suppressed growth of the pneumatized cellulae. We already demonstrated the normal process of pneumatization and the suppressive process of pneumatization in experimental studies using pigs. In that study, the process of suppression of the pneumatization was caused by the continuous inflammatory changes of the epithelium of the middle ear cavity, and the degree of the suppression of the pneumatization was caused by the degree and duration of the air cavity's inflammatory condition at the growing stage of the mastoid process. We also carried out the histological study of the normal process of pneumatization using 100 sides of human fetuses between 16th and 36th week of pregnancy. In these studies the bone metabolisms which were found in the normal processes of pneumatization in pigs and human fetuses were same, and the normal pneumatization occurred at the growing stage of the mastoid process. This growing period of the mastoid is from 0 to 6 months after birth in pigs, and 0 to 15 years in humans. So in humans, we can infer that the suppression of the growth of the cellulae is closely related to a persistent state of otitic inflammation like an otitis media with effusion in the early stages of growth. From these studies, in children who have otitis media with effusion, it will be possible to realize the previous middle ear pathology by the degree of the suppression of pneumatization on x-ray film.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Apófisis Mastoides/crecimiento & desarrollo , Otitis Media con Derrame/patología , Adolescente , Envejecimiento/patología , Aire , Niño , Preescolar , Humanos , Apófisis Mastoides/diagnóstico por imagen , Otitis Media con Derrame/terapia , RadiografíaAsunto(s)
Apófisis Mastoides/patología , Otitis Media con Derrame/patología , Adolescente , Adulto , Envejecimiento , Aire , Animales , Niño , Preescolar , Humanos , LactanteAsunto(s)
Neoplasias de las Glándulas Salivales/terapia , Adulto , Anciano , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Neoplasias de la Parótida/terapia , Neoplasias de las Glándulas Salivales/patología , Neoplasias de la Glándula Submandibular/terapiaAsunto(s)
Aire , Hueso Temporal/embriología , Animales , Humanos , Porcinos , Hueso Temporal/metabolismoRESUMEN
Nonenzymatic glycation of bovine serum albumin (BSA) was inhibited in vitro by some extracts of 34 kinds of spices. The methanol extract of thyme (Thymus vulgaris) had the most potent inhibitory activity among them. Chromatographic purification yielded four flavonoids, quercetin (1), eriodictyol (2), 5,6,4'-trihydroxy-7,8,3'-trimethoxyflavone (3), and cirsilineol (4). These known flavonoids suppressed the levels of advanced glycation end products (AGEs) and fructosamines, shown by the measurement of specific fluorescent groups and the reduction of nitroblue tetrazolium (NBT), respectively. The inhibitory activities were compared with those of other structure-related flavonoids and aminoguanidine.
Asunto(s)
Flavonoides/farmacología , Glucosa/metabolismo , Magnoliopsida/metabolismo , Albúmina Sérica Bovina/metabolismo , Flavonoides/química , Flavonoides/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Espectrometría de Masa Bombardeada por Átomos Veloces , Relación Estructura-ActividadRESUMEN
To clarify the relationship between the structure and inhibitory action toward ß-D-galactosidase (EC 3.2.1.21) of isoflavones and isoflavone glycosides, a number of polyhydroxyisoflavones, and the α-L-rhamnosides and ß-L-quinovosides of daidzein and genistein were synthesized. Among the polyhydroxyisoflavones, 2',3',4',7-tetrahydroxyisoflavone showed the strongest inhibitory activity (Ki = 26 × 10(-6) M). Among the glycosides, all the L-rhamnosides were strong inhibitors, of which genistein 4',7-di-O-α-L-rhamnoside was the strongest (Ki = 4.44 × 10(-6) M), while all the isoflavone ß-L-quinovosides were considerably weak or possessed no inhibition.
RESUMEN
The 7-O-[2-O-(alpha-L-Rhamnopyranosyl)-beta-L-quinovoside] of naringenin and of hesperetin, and their dihydrochalcone (DHC) derivatives were synthesized by the method of Koenigs-Knorr (Ag2CO3 and quinoline). The reaction of TMS ethers of naringenin and of hesperetin with each of the alpha-acetofluoro derivatives of D-glucose, L-rhamnose, 2-O-(alpha-L-rhamnopyranosyl)-L-rhamnose, and 2-O-(alpha-L-rhamnopyranosyl)-D-glucose (neohesperidose), using boron trifluoride etherate as an activator, yielded coupling products which, after deprotection, gave naringenin 4'-O-beta-D-glucoside, naringenin 4'-O-alpha-L-rhamnoside, naringenin 4'-O-[2-O-(alpha-L-rhamnopyranosyl)-alpha-L-rhamnoside], hesperetin 3'-O-[2-O-(alpha-L-rhamnopyranosyl)-alpha-L-rhamnoside], and naringenin 4'-O-beta-neohesperidoside, respectively. Catalytic hydrogenation of these flavanones gave the corresponding DHC derivatives. Hesperetin DHC 4'-O-[2-O-(alpha-L-rhamnopyranosyl-beta-L-quinovoside] was 300 times sweeter than sucrose, while the other compounds were bitter or tasteless.
Asunto(s)
Chalcona/análogos & derivados , Flavonoides/síntesis química , Glicósidos/síntesis química , Edulcorantes/síntesis química , Secuencia de Carbohidratos , Chalcona/síntesis química , Chalcona/química , Chalconas , Flavonoides/química , Glicósidos/química , Datos de Secuencia Molecular , Edulcorantes/química , GustoRESUMEN
The anticlastogenic effect of 12 structurally different flavonoids was investigated in whole body gamma-ray irradiated mice. Each flavonoid was administered to ICR male mice by a single gastric intubation (5 mumol/kg) 6 h before gamma-ray irradiation (1.5 Gy) and the frequency of micronucleated reticulocytes (MNRETs) in peripheral blood was determined. In order to elucidate the mechanism of the anticlastogenic effect of these flavonoids, their antioxidative activities were examined by the thiobarbituric acid method using methyl linoleate and Fenton's reagent (Fe2+/H2O2). Of the 12 flavonoids, luteolin had the most marked effect on reducing the frequencies of MNRETs and also inhibiting lipid peroxidation. However, quercetin tetramethylether, which has methoxy groups instead of hydroxyl groups at the 3,7,3',4'-positions, and phloretin with an open C-ring showed the least anticlastogenic and antioxidative activity. A good correlation (r = 0.717, P < 0.01) was observed between the anticlastogenic activity and the antioxidative activity of the 12 flavonoids. These results suggest that the radioprotective effect of flavonoids in mice may be attributed to the hydroxyl radical scavenging potency in a direct or an endogenous enzyme mediated manner.
Asunto(s)
Antimutagênicos/farmacología , Antioxidantes/farmacología , Flavonoides/farmacología , Protectores contra Radiación/farmacología , Animales , Rayos gamma , Masculino , Ratones , Ratones Endogámicos ICR , Micronúcleos con Defecto Cromosómico/efectos de los fármacosRESUMEN
In order to clarify the substrate specificity of the alpha-L-mannosidase activity of naringinase (Sigma), the following disaccharides and phenol glycosides were freshly prepared: methyl 2-O-(alpha-L-mannopyranosyl)-beta-D-glucoside (1), methyl 3-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (2), methyl 4-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (3), methyl 5-O-(alpha-L-mannopyranosyl)-beta-D-glucoside (4), methyl 6-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (5), 6-O-(alpha-L-mannopyranosyl)-D-galactose (6), p-nitrophenyl alpha-L-mannoside (7), and 4-methyl umbelliferone alpha-L-mannoside (8). These compounds, except for 3 and 5 were hydrolyzed with naringinase.