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1.
Toxicol Pathol ; 47(2): 138-149, 2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30587097

RESUMEN

The chemically induced accumulation of α2u-globulin protein in male rats causes specific renal lesions and subsequent nephropathy. Herein, we report additional parallel findings in the kidney of male rats consistent with obstructive and retrograde nephropathy. Kidney and urinary bladder samples were evaluated from Wistar rats treated with RG7129 for 2 week and 8 week and from an 8-week mechanistic study using females, intact and castrated males. Histopathological findings were present in intact males in all studies, including hyaline droplet accumulation and granular casts consistent with α2u-globulin nephropathy. In addition, tubular degeneration and regeneration, tubular changes extending from papilla to cortex, tubular dilation, and interstitial and luminal inflammation were observed consistent with retrograde and obstructive nephropathy. Renal and urinary lesions and their severity increased in a time- and dose-dependent manner. Urinalysis findings, including increases in leukocytes, protein, and in kidney biomarkers, kidney injury molecule 1 and clusterin, were present only in intact males. No treatment-related changes were observed in female rats or in castrated males. These results indicate that RG7129 induces α2u-globulin nephropathy, associated with retrograde and obstructive nephropathy secondary to precipitation in intact male rats only, constituting a species- and sex-specific syndrome that is not expected to occur in humans or other species.


Asunto(s)
alfa-Globulinas/metabolismo , Inhibidores Enzimáticos/toxicidad , Enfermedades Renales/inducido químicamente , Enfermedades Renales/metabolismo , Animales , Femenino , Enfermedades Renales/patología , Masculino , Fármacos Neuroprotectores/toxicidad , Ratas , Ratas Wistar
2.
Regul Toxicol Pharmacol ; 88: 125-137, 2017 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-28624430

RESUMEN

With the emergence of novel biotherapeutic formats and immunostimulatory biotherapeutics in cancer immunotherapy, an understanding of immune-complex (IC) mediated hypersensitivity reactions in toxicology studies - and their differentiation from pharmacology - remains key to the preclinical evaluation of these drugs. In this review we provide an in-depth evaluation and comparison of case examples where IC-mediated hypersensitivity reactions were observed in cynomolgus monkeys. We provide details of the parameters evaluated in each study to substantiate and guide the interpretation of these findings. Five study cases (1 therapeutic protein, 4 monoclonal antibodies) are discussed for which effects ranged from minor to fatal. Common characteristics are the high incidence of clinical signs, detectable antidrug antibodies, and accelerated drug clearance up to virtual loss of exposure. In our experience, measurement of cytokine levels in vivo and detection of complement (split products) were supportive markers in situations where coagulopathy was suspected to play a role in the observed effects. Recommendations are outlined to prepare for root-cause analysis of suspected hypersensitivity reactions. Overall, a thorough analysis of the findings has helped to start clinical trials despite major findings. The hypersensitivity reactions with our human(ized) immunoglobulins have not proven to be predictive for humans.


Asunto(s)
Complejo Antígeno-Anticuerpo/inmunología , Terapia Biológica/efectos adversos , Hipersensibilidad/inmunología , Inmunoglobulina G/inmunología , Inmunoterapia/efectos adversos , Animales , Anticuerpos Monoclonales/inmunología , Citocinas/sangre , Humanos , Inmunoglobulina G/administración & dosificación , Macaca fascicularis
3.
Regul Toxicol Pharmacol ; 86: 221-230, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28322894

RESUMEN

The nonclinical safety evaluation of therapeutic drug candidates is commonly conducted in two species (rodent and non-rodent) in keeping with international health authority guidance. Biologic drugs typically have restricted species cross-reactivity, necessitating the evaluation of safety in non-human primates and thus limiting the utility of lower order species. Safety studies of cross-reactive ocular biologic drug candidates have been conducted in rabbits as a second toxicology species, despite the fact that rabbits are not a rodent species. Such studies are often confounded by the development of anti-drug antibodies and severe ocular inflammation, the latter requiring studies to be terminated prematurely for animal welfare reasons. Notably, these confounding factors preclude the interpretation of safety. Nonclinical toxicology programs should be designed with consideration of ethical animal use and 3Rs principles (Replacement, Reduction and Refinement). The experience of several pharmaceutical sponsors, demonstrating that toxicology studies of ocular (intravitreal and topical ocular) biologic drug candidates in the rabbit are of limited interpretive value, calls into question the utility of such studies in this species and indicates that such studies should not be conducted.


Asunto(s)
Productos Biológicos/efectos adversos , Evaluación Preclínica de Medicamentos/métodos , Oftalmopatías/inmunología , Conejos , Animales , Ojo/inmunología , Inflamación/inmunología , Especificidad de la Especie
4.
J Biophotonics ; 17(2): e202300274, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-37795556

RESUMEN

Supervised deep learning (DL) algorithms are highly dependent on training data for which human graders are assigned, for example, for optical coherence tomography (OCT) image annotation. Despite the tremendous success of DL, due to human judgment, these ground truth labels can be inaccurate and/or ambiguous and cause a human selection bias. We therefore investigated the impact of the size of the ground truth and variable numbers of graders on the predictive performance of the same DL architecture and repeated each experiment three times. The largest training dataset delivered a prediction performance close to that of human experts. All DL systems utilized were highly consistent. Nevertheless, the DL under-performers could not achieve any further autonomous improvement even after repeated training. Furthermore, a quantifiable linear relationship between ground truth ambiguity and the beneficial effect of having a larger amount of ground truth data was detected and marked as the more-ground-truth effect.


Asunto(s)
Aprendizaje Profundo , Humanos , Tomografía de Coherencia Óptica/métodos , Sesgo de Selección , Algoritmos
5.
Sci Rep ; 13(1): 5797, 2023 04 09.
Artículo en Inglés | MEDLINE | ID: mdl-37032376

RESUMEN

Cynomolgus monkeys (Macaca fascicularis) are commonly used in pre-clinical ocular studies. However, studies that report the morphological features of the macaque retina are based only on minimal sample sizes; therefore, little is known about the normal distribution and background variation. This study was conducted using optical coherence tomography (OCT) imaging to investigate the variations in retinal volumes of healthy cynomolgus monkeys and the effects of sex, origin, and eye side on the retinal volumes to establish a comprehensive reference database. A machine-learning algorithm was employed to segment the retina within the OCT data (i.e., generated pixel-wise labels). Furthermore, a classical computer vision algorithm has identified the deepest point in a foveolar depression. The retinal volumes were determined and analyzed based on this reference point and segmented retinal compartments. Notably, the overall foveolar mean volume in zone 1, which is the region of the sharpest vision, was 0.205 mm3 (range 0.154-0.268 mm3), with a relatively low coefficient of variation of 7.9%. Generally, retinal volumes exhibit a relatively low degree of variation. However, significant differences in the retinal volumes due to the monkey's origin were identified. Additionally, sex had a significant impact on the paracentral retinal volume. Therefore, the origin and sex of cynomolgus monkeys should be considered when evaluating the macaque retinal volumes based on this dataset.


Asunto(s)
Aprendizaje Profundo , Tomografía de Coherencia Óptica , Animales , Macaca fascicularis , Tomografía de Coherencia Óptica/métodos , Retina/diagnóstico por imagen , Algoritmos
7.
Sci Rep ; 12(1): 13276, 2022 08 02.
Artículo en Inglés | MEDLINE | ID: mdl-35918392

RESUMEN

Cynomolgus monkeys exhibit human-like features, such as a fovea, so they are often used in non-clinical research. Nevertheless, little is known about the natural variation of the choroidal thickness in relation to origin and sex. A combination of deep learning and a deterministic computer vision algorithm was applied for automatic segmentation of foveolar optical coherence tomography images in cynomolgus monkeys. The main evaluation parameters were choroidal thickness and surface area directed from the deepest point on OCT images within the fovea, marked as the nulla with regard to sex and origin. Reference choroid landmarks were set underneath the nulla and at 500 µm intervals laterally up to a distance of 2000 µm nasally and temporally, complemented by a sub-analysis of the central bouquet of cones. 203 animals contributed 374 eyes for a reference choroid database. The overall average central choroidal thickness was 193 µm with a coefficient of variation of 7.8%, and the overall mean surface area of the central bouquet temporally was 19,335 µm2 and nasally was 19,283 µm2. The choroidal thickness of the fovea appears relatively homogeneous between the sexes and the studied origins. However, considerable natural variation has been observed, which needs to be appreciated.


Asunto(s)
Aprendizaje Profundo , Tomografía de Coherencia Óptica , Animales , Coroides/diagnóstico por imagen , Fóvea Central/diagnóstico por imagen , Humanos , Macaca fascicularis , Tomografía de Coherencia Óptica/métodos
8.
J Biochem Mol Toxicol ; 25(3): 183-94, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-21671310

RESUMEN

We have analyzed gene expression and histopathology of rat liver treated with a histamine-3 receptor inverse agonist under development for the treatment of obesity 24 h after a single acute administration. While histopathology did not identify a clear liver toxicity, analysis of gene changes strongly suggested the development of toxicity. This prediction was confirmed in a 2-week repeat-dose rat study where prominent liver pathology occurred, while gene changes that lead to the prediction persisted. A subset of these genes was analyzed in vitro in both rat and human hepatocytes to reveal the potential relevancy of the findings for the situation in humans. This comprehensive analysis of the development compound at the gene expression level allowed interpretation of findings of the follow-up compound in a frontloaded 24-h single-dose acute study that was initiated before regular 2-week repeat-dose studies started. The high similarity of the follow-up compound to the lead compound based on gene expression lead to the immediate termination of the development program for this compound series. Our data demonstrate the value of genomics-based early toxicity prediction in short-term in vivo studies for the characterization of compounds to allow prioritization and selection of suited candidates before compound-, animal-, and cost-intensive longer term studies are undertaken.


Asunto(s)
Descubrimiento de Drogas , Regulación de la Expresión Génica , Hígado/metabolismo , Preparaciones Farmacéuticas/análisis , Pruebas de Toxicidad/métodos , Animales , Células Cultivadas , Colesterol/biosíntesis , Genómica , Glutatión/metabolismo , Hepatocitos/metabolismo , Humanos , Masculino , Análisis de Secuencia por Matrices de Oligonucleótidos , Ratas , Ratas Wistar , Factores de Tiempo
9.
Comp Med ; 70(2): 145-151, 2020 04 01.
Artículo en Inglés | MEDLINE | ID: mdl-32164796

RESUMEN

Cynomolgus macaques are an important and commonly used species in preclinical toxicology studies, but structural reports of in vivo retinal findings are rare in this species. The purpose of this study was to diminish this gap and document optical coherence tomography and scanning laser ophthalmoscopy imaging data in the healthy posterior pole of cynomolgus monkeys' eyes at predose examinations. The current study is a retrospective assessment of baseline spectral domain OCT data obtained from the 768 eyes of 384 cynomolgus monkeys (192 males and 192 females) of Mauritian origin. The data set was obtained from studies conducted over a 4-y period in the context of ocular safety evaluations of various compounds under preclinical development. The most prevalent findings were the presence of Bergmeister papilla and intravitreal hyperreflective spots. Less common findings included disorganization of retinal zones, abnormalities of the retinal vasculature, partial posterior vitreous detachment, and abnormally shaped foveal pits. Thoughtful consideration of these physiologic findings will aid in distinguishing normal features from toxic outcomes in future preclinical ophthalmic studies.


Asunto(s)
Enfermedades de los Monos/diagnóstico por imagen , Enfermedades de la Retina/veterinaria , Animales , Femenino , Macaca fascicularis , Masculino , Oftalmoscopía/veterinaria , Enfermedades de la Retina/diagnóstico por imagen , Estudios Retrospectivos , Tomografía de Coherencia Óptica/veterinaria
10.
PLoS One ; 14(10): e0222850, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31589624

RESUMEN

The purpose of this study was to measure central macular thickness in an unprecedented number of cynomolgus monkeys. Macular thickness was measured with Heidelberg spectral-domain OCT in 320 eyes of healthy and treatment-naïve cynomolgus monkeys (80 males and 80 females). The macula was successfully measured in all 320 eyes. Macular thickness was not significantly different between the sexes. The mean central macular thickness was 244 µm (+/- 21 µm). Macular thicknesses in the quadrants were 327 +/-17 µm (temporal inner), 339 +/- 17 µm (inferior inner), 341 +/- 14 µm (superior inner), 341 +/-18 µm (nasal inner), and 299 +/- 20 µm (temporal outer), 320 +/- 16 µm (superior outer), 332 +/-23 µm (inferior outer), and 337 +/-18 µm (nasal outer). Highly significant differences between the nasal and temporal quadrants were detected. This study successfully demonstrated the feasibility of retinal thickness measurements in healthy cynomolgus monkeys. The present findings indicate that the macula is thicker in cynomolgus monkeys than in humans and provide important normative data for future studies.


Asunto(s)
Mácula Lútea/diagnóstico por imagen , Tomografía de Coherencia Óptica/métodos , Animales , Femenino , Macaca fascicularis , Masculino , Retina/diagnóstico por imagen
11.
Brain Res ; 1083(1): 50-60, 2006 Apr 14.
Artículo en Inglés | MEDLINE | ID: mdl-16545783

RESUMEN

A large body of experimental evidence suggests that the basal ganglia circuitry may be part of a remote control system modulating the spread of epileptic seizures. In the kindling model of temporal lobe epilepsy, this endogenous inhibitory control mechanism seems to be impaired. Neurochemical and neurophysiological studies have indicated that the activity of the GABAergic projection from the striatum to the substantia nigra pars reticulata is reduced in kindled rats, but the exact mechanisms involved in this observation are not known. Possible explanations include a kindling-induced loss of striatal GABAergic projection neurons to the substantia nigra or enhanced inhibition of these neurons by GABAergic interneurons. In the present experiments, the GABAergic system of the striatum (caudate-putamen) of amygdala-kindled rats and controls was studied immunohistochemically with a monoclonal antibody to GABA and with nonisotopic in situ hybridization with cRNA probes selective for glutamic acid decarboxylase 65 (GAD65) and GAD67, respectively. Compared to sham controls, an increased density of neurons heavily labeled for GAD67 mRNA was observed in the anterior striatum of kindled rats when cells were counted 6 weeks after the last kindled seizure. This subgroup of striatal GABAergic neurons has been suggested previously to correspond to the medium-sized aspiny interneurons in the striatum, indicating that kindling is associated with an increased activity of these neurons. Our previous finding of reduced GAD and GABA levels in synaptosomes isolated from the substantia nigra of kindled rats together with the present observation of increased density of GABAergic striatal interneurons in such rats suggest that kindling affects the regulation of the GABAergic projections from the striatum to the substantia nigra rather than directly damaging GABAergic neurons in the striatum.


Asunto(s)
Amígdala del Cerebelo/metabolismo , Cuerpo Estriado/metabolismo , Vías Nerviosas/metabolismo , Neuronas/metabolismo , Sustancia Negra/metabolismo , Ácido gamma-Aminobutírico/metabolismo , Amígdala del Cerebelo/fisiopatología , Animales , Cuerpo Estriado/fisiopatología , Modelos Animales de Enfermedad , Epilepsia del Lóbulo Temporal/genética , Epilepsia del Lóbulo Temporal/metabolismo , Epilepsia del Lóbulo Temporal/fisiopatología , Femenino , Glutamato Descarboxilasa/genética , Glutamato Descarboxilasa/metabolismo , Inmunohistoquímica , Isoenzimas/genética , Isoenzimas/metabolismo , Excitación Neurológica , Inhibición Neural/genética , Vías Nerviosas/fisiopatología , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Sustancia Negra/fisiopatología
12.
Brain Res ; 1025(1-2): 203-9, 2004 Oct 29.
Artículo en Inglés | MEDLINE | ID: mdl-15464761

RESUMEN

GABAergic inhibition of the substantia nigra pars reticulata (SNR) has been shown to suppress seizures in most models of epilepsy, including the amygdala-kindling model of temporal lobe epilepsy (TLE). A dysfunction of this seizure gating mechanism of the SNR may lead to facilitation of seizure propagation in such models. In post-status epilepticus models of TLE, GABAergic neurons in the SNR are damaged, but it is not known whether such damage also occurs in kindling. By using stereological techniques for cell counting in amygdala-kindled rats, we determined the density of SNR neurons that were labeled for GABA by immunohistochemistry or for the two isoforms of the GABA-synthesizing enzyme glutamate decarboxylase (GAD), GAD65 and GAD67, by in situ hybridization (ISH). In addition, GABA neurons in the basolateral amygdala (BLA) were counted. While there was a significant reduction of GAD65 mRNA expressing neurons in the BLA of kindled rats, no alteration in the density of neurons was observed in the anterior or posterior SNR when cells were counted 6 weeks after the last kindled seizure. Our previous finding of reduced GAD and GABA levels in synaptosomes isolated from the SN of kindled rats together with the present observation of unchanged density of SNR neurons in such rats suggest that kindling affects the GABAergic projections from the striatum or globus pallidus to the SNR rather than directly affecting GABA neurons in the SNR.


Asunto(s)
Amígdala del Cerebelo/citología , Excitación Neurológica/metabolismo , Neuronas/citología , Sustancia Negra/citología , Ácido gamma-Aminobutírico/metabolismo , Amígdala del Cerebelo/metabolismo , Animales , Femenino , Excitación Neurológica/patología , Neuronas/metabolismo , Ratas , Ratas Wistar , Sustancia Negra/metabolismo
13.
Basic Clin Pharmacol Toxicol ; 111(1): 50-7, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-22309322

RESUMEN

Oseltamivir is widely used for the treatment and prophylaxis of influenza. Renewed interest in the central nervous system (CNS) tolerability profile of oseltamivir has been triggered by the reports of neuropsychiatric adverse events in patients with influenza. In addition, a recent pre-clinical study in rodents suggested a hypothermic effect of oseltamivir. The current studies investigated the CNS effects, body temperature effect and toxicokinetic profile of oseltamivir in rats. The CNS/temperature study included three groups receiving oseltamivir (500, 763 and 1000 mg/kg free base by oral gavage), one vehicle/control group and one reference group (D-amphetamine, 10 mg/kg). CNS parameters (behaviour, motor activity and co-ordination and sensory/motor reflex responses) and rectal temperature were measured at baseline and at five intervals until 8 hr after dosing. In the toxicokinetic study, rats received oseltamivir by oral gavage at 763 or 1000 mg/kg free base. Plasma, cerebrospinal fluid (CSF) and perfused brain concentrations of oseltamivir and its active metabolite, oseltamivir carboxylate (OC), were measured until 8 hr after dosing. Median scores for CNS parameters were similar in controls and animals receiving oseltamivir at all time points. Oseltamivir had no physiologically relevant effect on body temperature, but induced a short-lived and small dose-independent decrease in temperature in all active treatment groups at 1 hr after dosing only. Plasma concentrations of OC were higher than of oseltamivir, but the reverse was true in CSF and brain. CNS penetration was low for both moieties. In rats, oseltamivir at supratherapeutic doses up to 1000 mg/kg free base did not exert any effects on CNS function or hypothermic effects and led to limited CNS exposure, resulting in large safety margins.


Asunto(s)
Antivirales/administración & dosificación , Sistema Nervioso Central/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Hipotermia , Oseltamivir/administración & dosificación , Administración Oral , Animales , Antivirales/efectos adversos , Antivirales/sangre , Antivirales/líquido cefalorraquídeo , Temperatura Corporal/efectos de los fármacos , Peso Corporal/efectos de los fármacos , Encéfalo/metabolismo , Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos/metabolismo , Masculino , Oseltamivir/efectos adversos , Oseltamivir/análogos & derivados , Oseltamivir/sangre , Oseltamivir/líquido cefalorraquídeo , Ratas , Ratas Sprague-Dawley
14.
J Med Chem ; 52(13): 3855-68, 2009 Jul 09.
Artículo en Inglés | MEDLINE | ID: mdl-19456097

RESUMEN

Obesity is a major risk factor in the development of conditions such as hypertension, hyperglycemia, dyslipidemia, coronary artery disease, and cancer. Several pieces of evidence across different species, including primates, underscore the implication of the histamine 3 receptor (H(3)R) in the regulation of food intake and body weight and the potential therapeutic effect of H(3)R inverse agonists. A pharmacophore model, based on public information and validated by previous investigations, was used to design several potential scaffolds. Out of these scaffolds, the 5-hydroxyindole-2-carboxylic acid amide appeared to be of great potential as a novel series of H(3)R inverse agonist. Extensive structure-activity relationships revealed the interconnectivity of microsomal clearance and hERG (human ether-a-go-go-related gene) affinity with lipophilicity, artificial membrane permeation, and basicity. This effort led to the identification of compounds reversing the (R)-alpha-methylhistamine-induced water intake increase in Wistar rats and, further, reducing food intake in diet-induced obese Sprague-Dawley rats. Of these, the biochemical, pharmacokinetic, and pharmacodynamic characteristics of (4,4-difluoropiperidin-1-yl)[1-isopropyl-5-(1-isopropylpiperidin-4-yloxy)-1H-indol-2-yl]methanone 36 are detailed.


Asunto(s)
Amidas/química , Agonistas de los Receptores Histamínicos/uso terapéutico , Indoles/química , Obesidad/tratamiento farmacológico , Receptores Histamínicos H3/efectos de los fármacos , Amidas/farmacocinética , Amidas/uso terapéutico , Animales , Permeabilidad de la Membrana Celular , Biología Computacional , Diseño de Fármacos , Agonistas de los Receptores Histamínicos/farmacocinética , Agonistas de los Receptores Histamínicos/farmacología , Interacciones Hidrofóbicas e Hidrofílicas , Indoles/farmacocinética , Indoles/uso terapéutico , Ratas , Ratas Sprague-Dawley , Ratas Wistar , Relación Estructura-Actividad
16.
Neurobiol Aging ; 28(9): 1421-35, 2007 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-16872721

RESUMEN

Intraneuronal alpha-synuclein (alphaSYN) inclusions constitute the hallmark lesions of a number of neurodegenerative diseases, including Parkinson's disease and dementia with Lewy bodies. In a transgenic mouse model expressing mutant [A30P]alphaSYN under control of the pan-neuronal Thy1 promoter, motor impairment became significant beyond 17 months of age. Cognitive performance was measured in the Morris water maze and upon fear conditioning. At 4 months of age, transgenic mice performed like controls. However, performance in these tasks was significantly impaired in (Thy1)-h[A30P]alphaSYN mice at 12 months of age. After completion of the cognition tests, mice were sacrificed and the regional distribution of neuropathology was examined. In contrast to 4 months old animals, 12 months old transgenic mice showed alpha-synucleinopathy in several brain regions, including the central nucleus of the amygdala, which is involved in cognitive behavior of mice, and is susceptible to alphaSYN pathology in human patients. Thus, age-dependent fibrillization of alphaSYN in specific cortical regions concomitant with cognitive decline may reflect dementia with Lewy bodies in a transgenic mouse model.


Asunto(s)
Amígdala del Cerebelo/patología , Trastornos del Conocimiento/patología , Trastornos del Conocimiento/fisiopatología , Enfermedades Neurodegenerativas , alfa-Sinucleína/genética , Factores de Edad , Análisis de Varianza , Animales , Reacción de Prevención/fisiología , Conducta Animal , Condicionamiento Clásico/fisiología , Modelos Animales de Enfermedad , Endopeptidasa K/metabolismo , Miedo , Hibridación in Situ , Aprendizaje por Laberinto/fisiología , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Actividad Motora/genética , Enfermedades Neurodegenerativas/complicaciones , Enfermedades Neurodegenerativas/genética , Enfermedades Neurodegenerativas/patología , Examen Neurológico , Desempeño Psicomotor , Prueba de Desempeño de Rotación con Aceleración Constante
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