Advanced Glycation End Product Inhibition by Alkaloids from Ocotea paranapiacabensis for the Prevention of Skin Aging.

A bioassay-guided study aiming at identifying inhibitors of the glycation process on the leaves of Ocotea paranapiacabensis afforded four benzylisoquinoline alkaloids (1-4), with 1 and 2 identified as new naturals products, while 3 and 4 were previously described in the literature, with 3 being identified as magnocurarine. Purification was performed by column chromatography and high-performance liquid chromatography. The structures of the isolated compounds were elucidated by spectroscopic methods including UV, NMR, and HRMS. The process of skin aging has been recently associated with advanced glycation end products (AGEs), and strategies inhibiting their formation have been addressed by pharmaceutical companies for the development of novel antiaging compounds. Alkaloids 1-4 were evaluated for their potential to inhibit AGE formation and showed inhibition of 62.9%, 83.3%, 26.1%, and 98.2% (150 µM), respectively. The antiaging potential of compounds 1 and 4 were evaluated with a reconstructed human skin model in vitro, and results showed a decrease in dermis contraction (8.7% and 4.2% respectively for 1 and 4) when compared to the glycated control (57.4%). Additionally, absorption, distribution, metabolism, and excretion (ADME) and toxicity properties were predicted using in silico methods, and the results were considered significantly promising for alkaloids 1 and 4 to continue the development of these alkaloids with skincare properties.

Atendimento em enfermaria psiquiátrica: concepções dos profissionais de saúde / Asistencia en un servicio de psiquiatría en hospital: concepciones de los profesionales de la salud / Attendance in psychiatric ward: healthcare professionals' conceptions

Buscou-se compreender as concepções de profissionais de uma enfermaria psiquiátrica referentes à organização do serviço e terapêutica oferecida, no que tange a aproximações e distanciamentos dos ideais da Reforma Psiquiátrica. A abordagem foi qualitativa e o tratamento dos dados baseou-se na técnica de Análise de Conteúdo Temática. Aplicou-se um questionário aberto, com temáticas referentes ao tratamento oferecido, planejamento e avaliação das ações, participação do usuário em seu tratamento, continuidade da assistência após a internação e a percepção dos profissionais sobre a enfermaria psiquiátrica. Participaram sete profissionais, sendo eles da área da enfermagem, psicologia, serviço social, terapia ocupacional e psiquiatria. De acordo com os resultados, os profissionais têm incorporado conceitos da Reforma Psiquiátrica; no entanto, encontram impasses, pois o cuidado ainda se mostra fragmentado e há espaços subutilizados para a comunicação da equipe. É essencial repensar formas de otimização do trabalho em equipe multiprofissional, com o propósito de proporcionar um cuidado integral.(AU)

This study intended to understand the psychiatric ward professionals' conceptions concerning the organization of the provided service and therapeutics, with respect to the approach and distancing from the Psychiatric Reform ideals. The qualitative approach was adopted and the data was processed by using the Thematic Content Analysis. The data collection was performed by applying an open questionnaire, approaching themes related to the treatment, planning and evaluation of the actions, participation of the user in his/her treatment, continuity of the assistance after the admission period and professionals' perception about the psychiatric ward. The sample comprised seven professionals from the nursing, psychology, social service, occupational area and psychiatry areas. According to the results, the professionals have incorporated concepts resulting from the psychiatric reform; however, they find impasses, once the care is still fragmented and there are underused spaces for the team communication. With this respect, it would be important to rethink the multiprofessional team work optimization ways in order to provide an integral care.(AU)

Se buscó comprender las concepciones de los profesionales de un pabellón psiquiátrico sobre la organización del servicio y la terapia ofrecida, en cuanto a enfoques y distancias de los ideales de la Reforma Psiquiátrica. El enfoque fue cualitativo y el procesamiento de datos se basó en la técnica de Análisis de Contenido Temático. Se aplicó un cuestionario abierto, con temas relacionados con el tratamiento ofrecido, la planificación y evaluación de las acciones, la participación del usuario en su tratamiento, la continuidad de la atención tras la hospitalización y la percepción de los profesionales sobre el servicio de psiquiatría. Participaron siete profesionales de las áreas de enfermería, psicología, trabajo social, terapia ocupacional y psiquiatría. Según los resultados, los profesionales han incorporado conceptos de Reforma Psiquiátrica; sin embargo, se enfrentan a un callejón sin salida, ya que la atención aún está fragmentada y hay espacios infrautilizados para la comunicación del equipo. Es fundamental repensar formas de optimizar el trabajo en equipo multidisciplinario, con el propósito de brindar una atención integral.(AU)