[Effect of serum from patients with kerion celsi on organ culture of chick embryo skin]. / Azione dei sieri di pazienti affetti da kerion celsi su culture organotipiche di cute embrionale di pollo.

In the present work the Authors have studied the differentiation and the cellular multiplication of chick embryo skin incubated in vitro with normal human sera or with pathological human sera from patients affected by Kerion Celsi. In the following research the Authors have studied the skin structure by an optical and electron microscope and the thymidine 3H incorporation. The result demonstrates that the pathological human serum does not interfere with the multiplication and the differentiation of the skin in vitro, but displays a cytoaggressive action that induces a noticeable cytoplasmic vacuolation.

[Preliminary scanning electron microscope (SEM) observations of the rat renal parenchyma after microdissection]. / Prime osservazioni al S.E.M. del parenchima renale di ratto, do po microdissezione.

Rat kidneys weighting between 250 and 300 g. were treated in HCl. A single nephron observed through SEM shows that the above treatment doesn't interfere with the ultrastructural morphology of nephron.

[Action of human serum on differentiation of skin in vitro. Morphological data and study of the incorporation of labeled amino acids]. / Azione del siero umano sul differenziamento della cute in vitro. Dati morfologici e studio dell'incorporazione di aminoacidi marcati.

Epithelial-mesodermal tissue interactions have been shown to be required for normal cytodifferentiation of chick embryo skin. Six-day limb skin does not develop in a protein free chemically defined medium, but keratinization has been observed in medium containing chicken serum. In the present study the authors show that the addition of human serum may stimulate the in vitro differentiation of explants of six-day chick embryo skin. Human serum is able to support skin keratinization and this finding has been confirmed by histological and histochemical criteria. Synthesis of proteins in tissue cultures supplemented with human serum has been studied by use of labeled amino acids such as H3-Leucine and C14-Cystine. These incorporation studies show the existence of macromolecular factors in human serum which may be responsible for the observed skin differentiation.

[Heterotopic transplantation of rat liver. Preliminary observations with the scanning electron microscope (SEM)]. / Trapianto eterotopico di fegato di ratto. Prime osservazioni al S.E.M.

An eterotopic grafting of their livers has been made between two inbred rats. From 30 to 50 days later the livers has been observed through OM or SEM. In the first case (OM) there was evidence of vacuolar degeneration of liver cells gradually decreasing on the 50th day. In the latter case (SEM) the morphological appearance remained unchanged.

[Differentiation in vitro of chick-embryo skin in presence of histones. Analysis of protein synthesis in toto and of keratin in particular, with the use of labeled precursors]. / Studio del differenziamento in vitro della pelle di embrione di pollo in presenza di istoni. Analisi della sintesi proteica in toto e della cheratina in par ticolare con l'uso di precursori marcati.

Six-day limb skin from a chick embryo was cultured in vitro for seven days in a complete medium either supplemented or not with histones. At the end of the incubation period, the chick embryo skin cultured in the absence of histones was found to undergo keratinization, the converse being true for the limbs cultured in the presence of histones. In the latter, when H3-leucine and C14-cystine were added to the medium, a sharp decrease in the labeled amino acid incorporation was found.

[Further research on the differentiation in vitro of chick-embryo skin in the presence of histones]. / Ulteriori ricerche sul differenziamento in vitro della pelle di embrione di pollo in fresenza di istoni.

In the present study the authors have carried out further researches on the differentiation of six day limb skin from chick embryo cultured "in vitro" in a complete medium supplemented with histones. When histones were added to the medium in the first two days "in vitro", epidermal keratinization was not observed. However the addition of histones after four days "in vitro" did not interfere with epidermal differentiation.

[Organotypic culture of normal and neoplastic laryngeal mucosa]. / Cultura organotipica di mucosa laringea normale e neoplastica.

Mucous membrane's phragments from human normal and neoplastic larynx have been maintained in a organ culture system. A set of cultures has been prepared for optical microscope studies and a parallel set for incorporation studies using 3H Thymidine and 3H Leucine. The results show that there is a good incorporation of 3H Thymidine and a greater incorporation of 3H Leucine at the 7th day due to a differentiation in vitro with a new synthesis of cheratin. These data agree with the morphologic evidence of considerable horny structures in the pathological mucous membrane after 11 days at culture.

Inhibition of in vitro keratinization of chick embryo skin cultured with hepatectomized rat serum.

In the present study organ cultures of 6 day-old chick embryo matatarsal skin were used to determine the effect of hepatectomized rat serum in modulating epithelial differentiation. Six day-old chick embryo skin, maintained in organ culture for 6-7 days with a medium containing adult rat serum, was seem to undergo differentiation and keratinization faster then in vivo. Epidermis cultured for 6-7 days with a medium containing partially hepatectomized rat serum does not differentiate and the squamous layer is absent. Studies with 3H-cystine show a sharp decrease in the labelled amino-acids incorporation in the cultures added with serum obtained after partial hepatectomy.

Further researches on the action of hepatectomized rat serum on epithelial differentiation in chick embryo skin.

Skin explants obtained from 12-day old chick embryos, maintained in organ culture containing adult rat serum, show a lower epidermal growth in comparison with skin explants cultured with other adult animal serum; nevertheless the keratogeneous differentiation of the epidermal cell is evident. When the cultures are incubated with medium containing partially hepatectomized rat serum epidermal-cell differentiation and proliferation are absent.

[Incorporation of H3 uridine in chick embryo skin cultured in media containing histones]. / Incorporazione di urinina H3 in espianti di cute di embrione di pollo coltivati con istoni.

In this paper the authors have carried out further research on the differentiation of six-day limb skin from chick embryos cultured in vitro in mediam containing histones. When 3H-uridine is added to this modified medium, a decrease of 3H-uridine uptake and incorporation is observed. The authors conclude that in their system the histones not only interfere with the regulation of genetic transcription by inhibiting DNA-dependent RNA synthesis, but they also change cellular permeability.