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1.
Phys Rev Lett ; 126(20): 201102, 2021 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-34110215

RESUMEN

The measurement of the energy spectrum of cosmic ray helium nuclei from 70 GeV to 80 TeV using 4.5 years of data recorded by the Dark Matter Particle Explorer (DAMPE) is reported in this work. A hardening of the spectrum is observed at an energy of about 1.3 TeV, similar to previous observations. In addition, a spectral softening at about 34 TeV is revealed for the first time with large statistics and well controlled systematic uncertainties, with an overall significance of 4.3σ. The DAMPE spectral measurements of both cosmic protons and helium nuclei suggest a particle charge dependent softening energy, although with current uncertainties a dependence on the number of nucleons cannot be ruled out.

2.
Genet Mol Res ; 14(3): 8819-28, 2015 Aug 03.
Artículo en Inglés | MEDLINE | ID: mdl-26345813

RESUMEN

Insulin resistance is a key feature of obesity and type 2 diabetes mellitus (T2DM). Interaction of insulin with the insulin receptor (IR) leads to both its auto-phosphorylation and phosphorylation of tyrosine residues on the IR substrate (IRS) proteins, initiating the activation of intracellular signaling cascades. The metabolic effects of IRS are known to be mediated through pathways involving phosphatidyl-inositol 3-kinase (PI-3K), which result in the activation of Akt signaling. The C-terminal region of the IR ectodomain is required to facilitate the conformational changes that are required for high-affinity binding to insulin. Furthermore, the CH2 and CH3 domains in the Fc fragments of immunoglobulins are responsible for their binding to the Fc receptor, which triggers transcytosis. In this study, we created a fusion peptide of the C-terminal end of the human IR ectodomain with the IgG4 Fc fragment, including an intervening polyG fragment to ensure enough space for insulin binding. We named this new peptide "Yiminsu", meaning an insulin sensitizer. The results of our analyses show that Yiminsu significantly facilitates insulin signaling via the activation of Akt in hepatocytes in a dose- and time-dependent manner. Further studies are required to determine whether Yiminsu can act as an insulin sensitizer.


Asunto(s)
Diabetes Mellitus Tipo 2/tratamiento farmacológico , Fragmentos Fab de Inmunoglobulinas/farmacología , Inmunoglobulina G/farmacología , Insulina/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Proteínas Recombinantes de Fusión/farmacología , Animales , Células CHO , Línea Celular , Clonación Molecular/métodos , Cricetulus , Diabetes Mellitus Tipo 2/metabolismo , Relación Dosis-Respuesta a Droga , Hepatocitos/efectos de los fármacos , Hepatocitos/metabolismo , Humanos , Fragmentos Fab de Inmunoglobulinas/química , Fragmentos Fab de Inmunoglobulinas/genética , Inmunoglobulina G/química , Inmunoglobulina G/genética , Resistencia a la Insulina , Fosfatidilinositol 3-Quinasa/metabolismo , Fosforilación/efectos de los fármacos , Ingeniería de Proteínas , Estructura Terciaria de Proteína , Receptor de Insulina/metabolismo , Receptores Fc/metabolismo , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Transducción de Señal/efectos de los fármacos , Tirosina/metabolismo
3.
Genet Mol Res ; 13(2): 3060-8, 2014 Apr 17.
Artículo en Inglés | MEDLINE | ID: mdl-24782163

RESUMEN

MicroRNAs (miRNAs) are small non-coding RNA molecules that play key roles in the regulation of development processes of many tissues and organs at the post-transcriptional level. However, little is known about how they affect chicken gonadal development. We examined the expression of four miRNAs (miR-218, -200b, -196, and -206) in chicken embryonic gonads at embryonic days 3.5-6.5. Their target genes were predicted by miRDB, TargetScan and PicTar algorithms. The expression levels of these four miRNAs differed with sex to varying degrees; miR-200b was expressed at a significantly higher level in female gonads during the entire interval. The whole mount in situ hybridization result showed considerably higher expression of miR-200b in females than in males in E5.5 embryos. The miRNA target scanning results indicated several genes with functions in gonad development and gonad function. We conclude that miR-200b is involved in the regulation of gonad development and sexual differentiation of chicken embryos.


Asunto(s)
Regulación del Desarrollo de la Expresión Génica , MicroARNs/biosíntesis , Diferenciación Sexual/genética , Animales , Embrión de Pollo , Pollos/genética , Femenino , Perfilación de la Expresión Génica , Gónadas/crecimiento & desarrollo , Gónadas/metabolismo , Hibridación in Situ , Masculino , MicroARNs/genética , Ovario/crecimiento & desarrollo , Ovario/metabolismo
4.
Br Poult Sci ; 55(1): 37-43, 2014 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-24215365

RESUMEN

Abstract 1. Polymorphisms of the duck MSTN gene were investigated in 413 individuals by DNA sequencing and polymerase chain reaction restriction fragment length polymorphism. Four single nucleotide polymorphisms (G129A, C324T, A981G and C1002A), with A981G and C1002A completely linked, were found in the coding region. 2. Association analysis showed that different genotypes of all the identified SNPs were significantly associated with duck growth rate from week 5, 6 and 2 for G129A, C324T and A981G (C1002A), respectively. The greatest difference in body weight was 180 g at week 9, 106 g at week 8 and 123 g at week 8, respectively, for the three SNP's. 3. Linkage disequilibrium (LD) analysis indicated that C324T, A981G and C1002A were in strong LD. Nine main diplotypes from the reconstructed five main haplotypes were observed, and different diplotypes were significantly associated with growth rate from week 1. Birds with the h1h1 diplotype exhibited the largest body weight from week 1 onwards. 4. It was concluded that the duck MSTN gene was associated with body weight and is an important candidate gene for duck growth. traits and marker-assisted selection.


Asunto(s)
Patos/crecimiento & desarrollo , Patos/genética , Miostatina/genética , Polimorfismo de Nucleótido Simple , Animales , Secuencia de Bases , Femenino , Haplotipos , Desequilibrio de Ligamiento , Masculino , Datos de Secuencia Molecular , Miostatina/metabolismo , Reacción en Cadena de la Polimerasa/veterinaria , Polimorfismo Conformacional Retorcido-Simple
5.
Br Poult Sci ; 54(5): 567-74, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24286503

RESUMEN

1. Two splice variants of duck LRP8 were identified, one containing 8 ligand-binding repeats (LRP8-1) and the other containing only 7 repeats (LRP8-2). The two transcripts share ~71-91% nucleic acid identity and ~65-94% amino acid identity with their counterparts in other species. A phylogenetic tree based on amino acid sequences shows that duck LRP8 proteins are closely related to those of chicken, turkey and zebra finch. 2. The semi-quantitative reverse transcription polymerase chain reaction (RT-PCR )analysis indicates that the two transcripts are expressed in all the examined tissues, and the LRP8-1 transcript is more highly expressed in hypothalamus, ovary and pituitary gland than in other detected tissues. 3. Six single nucleotide polymorphisms (SNPs) were identified in the coding region. Association analysis demonstrated that the c.528C > T genotypes were associated with egg production (EP) (EP210d, EP300d and EP360d), age at laying the first egg (AFE) and body weight at sexual maturity (BWSM). The c.1371A > G genotypes were associated with egg production (EP210d, EP300d and EP360d). 4. The haplotypes of c.528C > T and c.1371A > G were associated with EP (EP210d, EP300d and EP360d), yolk weight (YW), albumen weight (AW), egg weight (EW), BWSM and the first egg weight (FEW). 5. Duck LRP8 gene was associated with some reproductive traits and is an important candidate gene for the genetic selection of improved reproductive traits.


Asunto(s)
Patos/fisiología , Regulación de la Expresión Génica/fisiología , Proteínas Relacionadas con Receptor de LDL/fisiología , Filogenia , Polimorfismo Conformacional Retorcido-Simple/fisiología , Reproducción/fisiología , Animales , Secuencia de Bases , Clonación Molecular , Cruzamientos Genéticos , Patos/genética , Femenino , Regulación de la Expresión Génica/genética , Estudio de Asociación del Genoma Completo , Haplotipos , Proteínas Relacionadas con Receptor de LDL/genética , Datos de Secuencia Molecular , Polimorfismo de Longitud del Fragmento de Restricción , Polimorfismo Conformacional Retorcido-Simple/genética , ARN/química , ARN/genética , Reproducción/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Alineación de Secuencia , Análisis de Secuencia de ADN
6.
Acta Biol Hung ; 64(2): 161-8, 2013 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23739885

RESUMEN

Doublesex and mab-3-related transcription factor 1 (Dmrt1) is a Z-linked gene that putatively determines the phenotype of gonads in birds. The sex differential expression of Dmrt1 was examined using wholemount in situ hybridization (WISH) in the urogenital systems during embryogenesis. The results revealed that Dmrt1 showed dimorphic expression in chicken gonads, which increased from day 6.5 to day 10.5. The expression of Dmrt1 in male (ZZ) gonads was not twice as much as in female (ZW) gonads, suggesting the existence of other regulatory mechanisms in addition to Z chromosome dosage effect.


Asunto(s)
Desarrollo Embrionario/fisiología , Ovario/embriología , Caracteres Sexuales , Testículo/embriología , Factores de Transcripción/biosíntesis , Animales , Embrión de Pollo , Pollos , Femenino , Regulación del Desarrollo de la Expresión Génica , Hibridación Fluorescente in Situ , Masculino , Ovario/citología , Testículo/citología
7.
Acta Biol Hung ; 63(4): 453-62, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23134602

RESUMEN

New technologies in gene transfer combined with experimental embryology make the chicken embryo an excellent model system for gene function studies. The techniques of in ovo electroporation, in vitro culture for ex ovo electroporation and retrovirus-mediated gene transfer have already been fully developed in chicken. Yet to our knowledge, there are no definite descriptions on the features and application scopes of these techniques. The survival rates of different in vitro culture methods were compared and the EGFP expression areas of different gene transfer techniques were explored. It was that the optimal timings of removing embryo for EC culture and Petri dish system was at E1.5 and E2.5, respectively; and optimal timing of injecting retrovirus is at E0. Results indicated that the EC culture, in ovo electroporation, the Petri dish system and retrovirus-mediated method are, respectively, suitable for the very early, early, late and whole embryonic stages in chicken. Comparison of different gene transfer methods and establishment of optimal timings are expected to provide a better choice of the efficient method for a particular experiment.


Asunto(s)
Electroporación , Técnicas de Transferencia de Gen , Vectores Genéticos , Retroviridae/genética , Animales , Embrión de Pollo , Técnicas de Cultivo de Embriones , Genes Reporteros , Proteínas Fluorescentes Verdes/biosíntesis , Proteínas Fluorescentes Verdes/genética , Inyecciones , Factores de Tiempo
8.
Zhonghua Er Ke Za Zhi ; 60(12): 1312-1316, 2022 Dec 02.
Artículo en Zh | MEDLINE | ID: mdl-36444436

RESUMEN

Objective: To evaluate the diagnostic value and application of 24 h multichannel intraluminal impedance-pH (24 h MII-pH) monitoring in children with gastroesophageal reflux disease (GERD). Methods: This is a cross-sectional study. From January 2013 to December, 2020, 417 patients who received 24 h MII-pH monitoring in Department of Gastroenterology of Children's Hospital Capital Institute of Pediatrics were included. According to results, these children were divided into the GERD and non-GERD groups. Furthermore, the 132 children with GERD who had gastroscopy were divided into the reflux esophagitis (RE) and non-erosive reflux disease (NE) groups to investigate the differences in their refluxes. Non-parametric Mann-Whitney U test or indepentent sample t test was used for comparisons between the groups. Results: Among the 417 children, 232 were males and 185 females, aged (7.3±3.9) years. The course of disease was 0.5 (0.1, 2.0) years. The main clinical symptoms included acid reflux (128 cases), vomiting (173 cases), abdominal pain (101 cases), and cough (76 cases). The 24 h MII-pH monitoring were positive in 243 children (58.3%, 243/417), which was higher than that by 24 h esophageal pH monitoring (43.6%, 182/417). The 24 h MII-pH monitoring results demonstrated significant differences in the episodes of acid reflux, weakly acidic reflux, non-acidic reflux, liquid reflux and mixed reflux between GERD and non-GERD groups (10 (4, 19) vs. 4 (1, 9) times/24 h, 14 (6, 32) vs. 7 (3, 13) times/24 h, 0 (0, 0) vs. 0 (0, 0) times/24 h, 19 (10, 34) vs. 8 (3, 14) times/24 h, and 6 (2, 12) vs. 3 (1, 5) times/24 h, Z=-6.96, -7.25, -5.62, -8.75, and -6.48, all P<0.05, respectively). Besides, the results also showed significant differences in Boix-Ochoa score, episodes of long reflux, course of long reflux, and episodes of weakly acidic reflux between the RE and NE groups (51.2 (21.4, 153.2) vs. 20.7 (12.1, 34.7), 5 (2, 10) vs. 1 (0, 4) times/24 h, 19 (7, 87) vs. 8 (3, 22) min, and 5 (2, 15) vs. 15 (6, 33) times/24 h, Z=-3.44, -3.41, -2.65, and -2.27, all P<0.05, respectively). Conclusion: 24 h MII-pH monitoring not only improves the detection rate of GERD in children, but also provides a possibility to early etiological diagnosis.


Asunto(s)
Reflujo Gastroesofágico , Femenino , Masculino , Humanos , Niño , Estudios Transversales , Impedancia Eléctrica , Reflujo Gastroesofágico/diagnóstico , Monitorización del pH Esofágico , Concentración de Iones de Hidrógeno
10.
Zhonghua Shao Shang Za Zhi ; 37(6): 546-554, 2021 Jun 20.
Artículo en Zh | MEDLINE | ID: mdl-34139835

RESUMEN

Objective: To investigate the role of Bruton's tyrosine kinase (BTK) in pyroptosis of intestinal cells caused by endotoxin/lipopolysaccharide (LPS) in scalded mice. Methods: The experimental research method was applied. One hundred and twenty-eight male C57BL/6 mice aged 6-8 weeks were divided into sham injury group, scald alone group, scald+LPS group, scald+LPS+3 mg/kg LFM-A13 group, scald+LPS+10 mg/kg LFM-A13 group, and scald+LPS+30 mg/kg LFM-A13 group. There were 8 mice in sham injury group, and there were 24 mice in the other 5 groups, respectively. Mice in 5 scald groups were inflicted with 10% total body surface area full-thickness scald on the back, and mice in sham injury group were sham injured on the back. At post injury hour (PIH) 0 (immediately), mice in sham injury group and scald alone group were intraperitoneally injected with normal saline, mice in scald+LPS group were intraperitoneally injected with LPS, and mice in scald+LPS+3 mg/kg LFM-A13 group, scald+LPS+10 mg/kg LFM-A13 group, and scald+LPS+30 mg/kg LFM-A13 group were intraperitoneally injected with LPS and LFM-A13 in corresponding doses. Mice in sham injury group were sacrificed at PIH 0 to collect serum and intestinal tissue, and 8 mice in each group of 5 scald groups were sacrificed at PIH 0, 12, and 24 to collect intestinal tissue and serum at PIH 12. Immunohistochemistry was used to detect phosphorylation of BTK in intestinal tissue of mice. Western blotting was used to detect the protein expressions of phosphorylated BTK (p-BTK), cleaved cysteine aspartic acid specific protease 1 (caspase-1), and cleaved caspase-11 in intestinal tissue of mice. Enzyme-linked immunosorbent assay method was used to detect interleukin-1ß (IL-1ß) in serum and intestinal tissue of mice. Data were statistically analyzed with one-way analysis of variance and least significant difference test. Results: There was no obvious phosphorylation of BTK in intestinal tissue of mice in 6 groups at PIH 0 and scald alone group at PIH 12 and 24. Phosphorylation of BTK in intestinal tissue of mice in scald+LPS group at PIH 12 and 24 were obviously increased compared with those in scald alone group. Phosphorylation of BTK in intestinal tissue of mice in scald+LPS+3 mg/kg LFM-A13 group, scald+LPS+10 mg/kg LFM-A13 group, and scald+LPS+30 mg/kg LFM-A13 group were obviously decreased compared with those in scald+LPS group, and the degrees of decline gradually increased with increase of dose in LFM-A13. Compared with (0.130±0.010) of sham injury group and (0.120±0.040 and 0.110±0.040) of scald alone group, protein expressions of p-BTK in intestinal tissue of mice in scald+LPS group at PIH 12 and 24 were obviously increased (0.470±0.090 and 0.430±0.080, P<0.01). Compared with those in scald+LPS group, protein expressions of p-BTK in intestinal tissue of mice in scald+LPS+3 mg/kg LFM-A13 group at PIH 24, and scald+LPS+10 mg/kg LFM-A13 group and scald+LPS+30 mg/kg LFM-A13 group at PIH 12 and 24 were obviously decreased (0.280±0.060, 0.300±0.120, 0.150±0.050, 0.280±0.090, 0.140±0.040, P<0.05 or P<0.01). Compared with those in scald+LPS+3 mg/kg LFM-A13 group, protein expressions of p-BTK in intestinal tissue of mice in scald+LPS+10 mg/kg LFM-A13 group and scald+LPS+30 mg/kg LFM-A13 group at PIH 24 were obviously decreased (P<0.01). Compared with those in sham injury group and scald alone group, protein expressions of cleaved caspase-1 and caspase-11 in intestinal tissue of mice in scald+LPS group were obviously increased at PIH 12 and 24 (P<0.01). Compared with those in scald+LPS group, protein expressions of cleaved caspase-1 at PIH 12 and cleaved caspase-11 at PIH 12 and 24 in intestinal tissue of mice in scald+LPS+3 mg/kg LFM-A13 group and protein expressions of cleaved caspase-1 and caspase-11 in intestinal tissue of mice in scald+LPS+10 mg/kg LFM-A13 group and scald+LPS+30 mg/kg LFM-A13 group at PIH 12 and 24 were obviously decreased (P<0.01). Compared with those in scald+LPS+3 mg/kg LFM-A13 group, protein expressions of cleaved caspase-1 and caspase-11 in intestinal tissue of mice in scald+LPS+10 mg/kg LFM-A13 group and scald+LPS+30 mg/kg LFM-A13 group at PIH 12 and 24 were obviously decreased (P<0.05 or P<0.01). At PIH 12, content of IL-1ß in intestinal tissue and serum of mice in scald+LPS group were obviously higher than those in sham injury group and scald alone group (P<0.01), and content of IL-1ß in intestinal tissue and serum of mice in scald+LPS+30 mg/kg LFM-A13 group were obviously lower than those in scald+LPS group (P<0.01). Conclusions: Phosphorylation of BTK is related to increases of cleaved caspase-1 and caspase-11 in intestinal tissue, and IL-1ß content in intestinal tissue and serum of scalded septic mice caused by LPS. Phosphorylation of BTK mediates intestinal cell pyroptosis of scalded mice caused by LPS. Inhibiting phosphorylation of BTK can alleviate intestinal cell pyroptosis of scalded mice, with protective effect on intestinal injury intestine.


Asunto(s)
Quemaduras , Lipopolisacáridos , Agammaglobulinemia Tirosina Quinasa , Animales , Endotoxinas , Intestinos , Masculino , Ratones , Ratones Endogámicos C57BL , Piroptosis , Ratas , Ratas Sprague-Dawley
11.
Zhonghua Er Ke Za Zhi ; 58(7): 570-575, 2020 Jul 02.
Artículo en Zh | MEDLINE | ID: mdl-32605341

RESUMEN

Objective: To analyze the clinical characteristics and efficacy of drug treatment in children with inflammatory bowel disease (IBD) at different ages of onset. Methods: The clinical data of 87 children with IBD admitted to Department of Gastroenterology in Children's Hospital, Capital Institute of Pediatrics from January 2009 to December 2018 were collected. The patients were divided into four groups according to the age of onset: 0 -<2 years old group (36 cases), 2 -<6 years old group (10 cases), 6 -<10 years old group (12 cases) and 10 -<18 years old group (29 cases). The clinical manifestations, laboratory examination, endoscopic findings, pathologic and genetic changes, and treatment were compared among different age groups with chi-square test or Fisher's exact text. Results: (1) A total of 87 patients were diagnosed with IBD, including 50 Crohn's disease (CD) (57%), 25 ulcerative colitis (UC) (29%) and 12 unclassified inflammatory bowel disease (IBD-U) (14%). (2) Patients with fever accounted for 78% (28/36) and 8/10 in the 0 -<2 years old group and 2 -<6 years old group, respectively. Patients with abdominal pain and perianal diseases accounted for 6% (2/36) and 47% (17/36) in the 0 -<2 years old group, and their proportions were significantly different among the four groups (χ(2)=8.369, 40.317 and 13.130, all P<0.05). (3) Leukocytosis, thrombocytosis and anemia were more common in the 0-<2 years old group, seen in 72% (26/36), 31% (11/36) and 81% (29/36), respectively. There were significant differences in the changes of complete blood count among the four groups (χ(2)=21.919, 8.095 and 11.520, all P<0.05). (4) Colonic involvement accounted for 85% (17/20) in the 0 -<2 years old CD patients. While in the CD patients over 6 years old, 61% (14/23) had inflammation of ileum and colon, with a significant difference compared to that in patients under 6 years old (19% (5/27) , χ(2)=9.455, P=0.003). Also, the location of bowel inflammation among the four groups were significantly different (χ(2)=21.120, P<0.01). (5) Noncaseating granulomas were found in 15 (30%) CD patients, and crypt abscess was found in 11 (44%) UC patients. (6) Among the 24 patients whose genes were analyzed by high throughput sequencing, 12 had pathogenic single gene mutation. (7) There were 25 patients treated with total enteral nutrition. Among the 25 patients treated with thalidomide, 20 (80%) had clinical remission or partial remission. Among the 19 CD patients treated with infliximab (IFX), 14 had clinical remission at the 6(th) week of treatment, and the proportion of remission maintenance at the 30(th) week of treatment was 12/14. (8) The rate of clinical remission or partial remission was 64% (23/36) in the 0 -<2 years old group, 8/10 in the 2 -<6 years old group, 11/12 in the 6 -<10 years old group, and 83% (24/29) in the 10 -<18 years old group. Conclusions: The proportion of CD was higher than that of UC in this study. Infant onset inflammatory bowel disease was more likely to present with perianal lesions, and was usually associated with leukocytosis, thrombocytosis and anemia, and has high possibility of single gene mutation. IFX may be effective in treating CD.


Asunto(s)
Colitis Ulcerosa , Enfermedad de Crohn , Enfermedades Inflamatorias del Intestino , Adolescente , Niño , Preescolar , Colitis Ulcerosa/tratamiento farmacológico , Colitis Ulcerosa/epidemiología , Enfermedad de Crohn/tratamiento farmacológico , Nutrición Enteral , Fármacos Gastrointestinales/uso terapéutico , Humanos , Lactante , Enfermedades Inflamatorias del Intestino/tratamiento farmacológico , Enfermedades Inflamatorias del Intestino/epidemiología , Infliximab/uso terapéutico , Talidomida/uso terapéutico
12.
Poult Sci ; 88(10): 2212-8, 2009 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-19762878

RESUMEN

The main objective of this study was to examine the diversity of meat quality traits among 5 chicken genotypes. The genotypes included 2 Chinese native breeds (Wenchang,WCH, and Xianju), 1 commercial broiler line (Avian, AV), 1 commercial layer line (Hy-Line Brown, HLB), and 1 Chinese commercial broiler line (Lingnanhuang, LNH) synthesized by exotic and native breeds, which were slaughtered at their market ages: 16, 7, 16, and 8 wk, respectively. The effects of genotype, muscle type, and sex on meat quality traits were examined. Birds from slow-growing genotypes (WCH, Xianju, and HLB) exhibited higher shear value, inosine-5'-monophosphate concentration, lower cook loss, and more fat than those from fast-growing genotypes (AV and LNH). Chickens from WCH possessed the lowest expressible moisture, cook loss, and the highest lipid (%) among the 3 slow-growing genotypes. The HLB birds were intermediate in expressible moisture and cook loss and lowest in lipid among all genotypes. The LNH cross birds were similar to AV broilers in most meat quality parameters, although they had a lower shear force value and higher fat content than AV broilers. Breast muscle had higher expressible moisture, shear force, protein (%), inosine-5'-monophosphate content, lower cook loss, and lipid (%) than leg muscle. Muscles from male chickens had higher expressible moisture than those from the females. Variability of meat quality characteristics is mainly related to genotype and muscle type differences.


Asunto(s)
Pollos/genética , Carne/normas , Músculo Esquelético/fisiología , Animales , Pollos/fisiología , Culinaria , Ácidos Grasos no Esterificados/análisis , Femenino , Genotipo , Inosina Monofosfato/análisis , Lípidos/análisis , Masculino , Distribución Aleatoria
13.
Zhonghua Er Ke Za Zhi ; 57(7): 520-525, 2019 Jul 02.
Artículo en Zh | MEDLINE | ID: mdl-31269551

RESUMEN

Objective: To analyze the clinical and genotypic characteristics of infantile inflammatory bowel disease (IBD). Methods: The age of onset, family history, clinical manifestations, and treatment effect were retrospectively analyzed in 39 infants (male 23 cases, female 16 cases) with IBD who were admitted to the Department of Gastroenterology in Children's Hospital, Capital Institute of Pediatrics from January 2007 to December 2017. Next generation sequencing (NGS) based on target gene panel was used for gene analysis in 17 patients. Results: The median age of onset was 0.5 (0.5, 1.0) month. The most common clinical symptoms included diarrhea (39, 100%), malnutrition (38, 97%), hematochezia (34, 87%), fever (25, 64%), and perianal diseases (24, 61%). Four children had associated family history. Among the 17 patients whose gene was analyzed, 10 were found to have the pathogenic gene variation, within whom 7 had interleukin-10 receptor α subunit (IL-10RA) mutation, 2 had CYBB heterozygous mutation, 1 had interleukin-10 receptor ß subunit (IL-10RB) mutation. The therapeutic medicine included mesalazine, steroids, and thalidomide. Eighteen children (46%) reached clinical remission (10 cases) or partial remission (8 cases). Conclusions: The incidence of single gene mutation in infants with IBD is high, with IL-10RA mutation as the most common. Refractory diarrhea and malnutrition may indicate infantile IBD.


Asunto(s)
Enfermedades Inflamatorias del Intestino/diagnóstico , Subunidad alfa del Receptor de Interleucina-10/genética , Subunidad beta del Receptor de Interleucina-10/genética , NADPH Oxidasa 2/genética , Niño , Femenino , Marcadores Genéticos , Genotipo , Humanos , Lactante , Enfermedades Inflamatorias del Intestino/genética , Interleucina-10 , Masculino , Mutación , Estudios Retrospectivos , Análisis de Secuencia de ADN
14.
Sci Adv ; 5(9): eaax3793, 2019 09.
Artículo en Inglés | MEDLINE | ID: mdl-31799401

RESUMEN

The precise measurement of the spectrum of protons, the most abundant component of the cosmic radiation, is necessary to understand the source and acceleration of cosmic rays in the Milky Way. This work reports the measurement of the cosmic ray proton fluxes with kinetic energies from 40 GeV to 100 TeV, with 2 1/2 years of data recorded by the DArk Matter Particle Explorer (DAMPE). This is the first time that an experiment directly measures the cosmic ray protons up to ~100 TeV with high statistics. The measured spectrum confirms the spectral hardening at ~300 GeV found by previous experiments and reveals a softening at ~13.6 TeV, with the spectral index changing from ~2.60 to ~2.85. Our result suggests the existence of a new spectral feature of cosmic rays at energies lower than the so-called knee and sheds new light on the origin of Galactic cosmic rays.

15.
Zhonghua Er Ke Za Zhi ; 56(10): 753-758, 2018 Oct 02.
Artículo en Zh | MEDLINE | ID: mdl-30293279

RESUMEN

Objective: To analyze the clinical features and interleukin-10 receptor gene mutations in six infants with very early onset inflammatory bowel disease (VEO-IBD). Methods: Four girls and two boys with VEO-IBD admitted to Children's Hospital Affiliated to Capital Institute of Pediatrics from June 2016 to September 2017 were reviewed. The clinical data including general condition, clinical symptoms, laboratory tests, and colonoscopy and pathological results were collected and analyzed. Interleukin-10 receptor α subunit (IL-10RA) gene was examined in all patients. Results: Persistent diarrhea and fever were the most common symptoms and were found within 1 month after birth in all 6 patients. Anemia, oral ulcer or perianal lesions and growth retardation were common concomitant symptoms. All patients had colonoscopy examination and the results showed multiple ulcers affecting the colon with biopsies revealing acute and chronic inflammation. Three patients were found to have cryptitis and crypt abscesses. Gene sequencing revealed IL-10RA gene mutations in all six patients, including 3 cases with homozygous mutations (one with c.537G>A and two with c.301C>T) and 3 heterozygous mutations (paternal c.301C>T in all cases; maternal c.299T>G, c.350G>A and c.537G>A, respectively) . After conventional treatment, one got clinical and pathological improvement according to colonoscopy, three improved clinically, one worsened and died, and one died of septic shock secondary to intestinal perforation. Conclusions: VEO-IBD is associated with IL-10RA mutation, usually with severe intestinal symptoms and significant extra-intestinal symptoms, as well as varied responses to conventional treatment. In our study, c.301C>T and c.537G>A are the most common mutations.


Asunto(s)
Enfermedades Inflamatorias del Intestino , Subunidad alfa del Receptor de Interleucina-10 , Mutación , Femenino , Heterocigoto , Humanos , Lactante , Enfermedades Inflamatorias del Intestino/complicaciones , Enfermedades Inflamatorias del Intestino/genética , Interleucina-10 , Subunidad alfa del Receptor de Interleucina-10/genética , Masculino , Estudios Retrospectivos
17.
Biochim Biophys Acta ; 1078(3): 329-35, 1991 Jul 12.
Artículo en Inglés | MEDLINE | ID: mdl-1859823

RESUMEN

A 14 kDa polypeptide in rat ileal cytosol has been identified as the major intestinal cytosolic bile acid-binding protein (I-BABP) by photoaffinity labeling with the radiolabeled 7,7-azo derivative of taurocholate (7,7-azo-TC). To further characterize I-BABP, the protein was purified by lysylglycocholate Sepharose 4B affinity and DE-52 anion-exchange chromatography. The purified I-BABP contained a single 14 kDa band on SDS-PAGE. The 14 kDa protein showed a 26-fold increase in binding affinity for [3H]7,7-azo-TC compared to cytosolic protein. Immunoblotting of protein fractions separated by affinity chromatography showed that neither liver fatty acid binding protein (L-FABP) nor intestinal fatty acid binding protein (I-FABP) bind to the affinity column and that the 14 kDa protein which bound to the column and was subsequently eluted with detergent did not cross-react with anti-L-FABP or anti-I-FABP. The 14 kDa protein labeled with [3H]7,7-azo-TC was radioimmunoprecipitated from cytosol by rabbit antiserum raised against purified I-BABP. I-BABP was shown to have a blocked N-terminus; however, its mixed internal sequence generated from cyanogen bromide-cleaved protein and amino acid composition indicated that it was related to (although clearly distinct from) both I-FABP and L-FABP. These studies have isolated a 14 kDa bile acid-binding protein from rat ileal cytosol which is immunologically and biochemically distinct from I-FABP and L-FABP.


Asunto(s)
Ácidos y Sales Biliares/metabolismo , Proteínas Portadoras/aislamiento & purificación , Citosol/química , Hidroxiesteroide Deshidrogenasas , Íleon/química , Glicoproteínas de Membrana , Secuencia de Aminoácidos , Animales , Cromatografía por Intercambio Iónico , Immunoblotting , Masculino , Datos de Secuencia Molecular , Peso Molecular , Ratas , Ratas Endogámicas
19.
Zhonghua Zhong Liu Za Zhi ; 10(3): 184-7, 1988 May.
Artículo en Zh | MEDLINE | ID: mdl-3219978

RESUMEN

The in vitro synthesis of secretory immunoglobulin A (SIgA) and carcinoembryonic antigen (CEA) was observed by tissue culture of biopsied gastric cancer tissue and gastric mucosa in other gastric diseases. The level of SIgA Synthesis in cultured gastric cancer tissue was lower than that in gastric mucosa in chronic atrophic gastritis, chronic superficial gastritis and normal stomach. The gastric mucosa of chronic gastritis can produce more SIgA than the normal gastric tissue, but the difference between chronic atrophic gastritis and chronic superficial gastritis was of no statistical significance. The CEA level was significantly higher in cancerous tissue than that in noncancerous ones, the amount of CEA synthesis by gastric mucosa in chronic atrophic gastritis was higher than that in chronic superficial gastritis and normal stomach. Well differentiated adenocarcinoma secreted much more SIgA and CEA than the poor-differentiated ones. The results suggest that the estimation of secretory function of SIgA and CEA be helpful for clinical diagnosis of gastric cancer.


Asunto(s)
Adenocarcinoma/inmunología , Antígeno Carcinoembrionario/análisis , Inmunoglobulina A Secretora/biosíntesis , Neoplasias Gástricas/inmunología , Técnicas de Cultivo , Femenino , Mucosa Gástrica/inmunología , Gastritis/inmunología , Humanos , Masculino , Persona de Mediana Edad , Células Tumorales Cultivadas
20.
Zhongguo Zhong Yao Za Zhi ; 25(5): 293-6, 2000 May.
Artículo en Zh | MEDLINE | ID: mdl-12512454

RESUMEN

OBJECTIVE: To investigate the inhibitory effect of Xinjierkang(XJEK) granules on virus myocarditis. METHODS: Using a model mouse of virus myocarditis induced by coxsackie virus B3m (CVB3m) and mouse toxic myocarditis induced by adriamycin, a model of arrhythmia induced by BaCl2 and CHCl3, a model of inflammation caused by egg white and agar, along with a dynamic test of cardiac blood flow and an inhibitory test of CVB3m in vitro. RESULTS: XJEK granules are efficacious in inhibiting CVB3m both in vitro and in vivo, protecting and curing virus myocarditis and toxic myocarditis in mice, reducing mouse death rate, serum level of LDH, AST and CK, titer of neutralizing antibodies, virus concentration of heart, and improving the abnormal ECG, pathological and ultrastructural damage of myocadium. The granules are also good for anti-inflammation, anti-myocardial ischemia, anti-arrhythmia, as well as for strengthening myocardiac contraction and increasing the serum IgG level. CONCLUSION: Xinjerkang granules possess an inhibitory effect on virus myocarditis and toxic myocarditis.


Asunto(s)
Antivirales/farmacología , Infecciones por Coxsackievirus/fisiopatología , Medicamentos Herbarios Chinos/farmacología , Enterovirus Humano B/efectos de los fármacos , Miocarditis/fisiopatología , Fitoterapia , Animales , Combinación de Medicamentos , Masculino , Ratones , Ratones Endogámicos BALB C , Miocarditis/virología , Plantas Medicinales , Distribución Aleatoria , Ratas , Ratas Wistar
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