The extent of the raphe in bicuspid aortic valves is associated with aortic regurgitation and aortic root dilatation.

BACKGROUND: The clinical course of bicuspid aortic valves (BAVs) is variable. Data on predictors of aortopathy and valvular dysfunction mainly focus on valve morphology. AIM: To determine whether the presence and extent of the raphe (fusion site of valve leaflets) is associated with the degree of aortopathy and valvular dysfunction in patients with isolated BAV and associated aortic coarctation (CoA). METHODS: Valve morphology and aortic dimensions of 255 BAV patients were evaluated retrospectively by echocardiography. RESULTS: BAVs with a complete raphe had a significantly higher prevalence of valve dysfunction (especially aortic regurgitation) than BAVs with incomplete raphes (82.9 vs. 66.7 %, p = 0.01). Type 1A BAVs (fusion of right and left coronary leaflets) and complete raphe had larger aortic sinus diameters compared with the rest of the population (37.74 vs. 36.01, p = 0.031). Patients with CoA and type 1A BAV had significantly less valve regurgitation (13.6 vs. 55.8 %, p < 0.001) and smaller diameters of the ascending aorta (33.7 vs. 37.8 mm, p < 0.001) and aortic arch (25.8 vs. 30.2 mm, p < 0.001) than patients with isolated BAV. CONCLUSIONS: Type 1A BAV with complete raphe is associated with more aortic regurgitation and root dilatation. The majority of CoA patients have incomplete raphes, associated with smaller aortic root diameters and less valve regurgitation.

Normal and abnormal development of the aortic wall and valve: correlation with clinical entities.

Dilation of the wall of the thoracic aorta can be found in patients with a tricuspid (TAV) as well as a bicuspid aortic valve (BAV) with and without a syndromic component. BAV is the most common congenital cardiovascular malformation, with a population prevalence of 0.5-2 %. The clinical course is often characterised by aneurysm formation and in some cases dissection. The non-dilated aortic wall is less well differentiated in all BAV as compared with TAV, thereby conferring inherent developmental susceptibility. Furthermore, a turbulent flow, caused by the inappropriate opening of the bicuspid valve, could accelerate the degenerative process in the aortic wall. However, not all patients with bicuspidy develop clinical complications during their life. We postulate that the increased vulnerability for aortic complications in a subset of patients with BAV is caused by a defect in the early development of the aorta and aortic valve. This review discusses histological and molecular genetic aspects of the normal and abnormal development of the aortic wall and semilunar valves. Aortopathy associated with BAV could be the result of a shared developmental defect during embryogenesis.

Exploring the impact of sleep on emotional and physical well-being in professional cricketers: a cohort study over an in-season training period.

Background: Professional athletes navigate a multitude of unique challenges associated to sport-specific factors (e.g., training, travel and competition) and non-sport factors (e.g., performance pressure, stress and anxiety) that can interfere with healthy sleep behaviors. Sleep plays a key role in proper biopsychosocial development as well as short- and long-term biological, physical, psychological, and cognitive health. As poor sleep quality is known to impair proper brain function, this study aimed to investigate the effect of sleep quality on a professional athlete's ability to train, recover, and perform, as well as their overall emotional and physical well-being. Methods: A cohort study was performed in 40 professional male cricket athletes from the Dutch national cricket team (mean age 26.5 ± 5.1 years). The athletes were monitored across a 22 weeks in-season training period. Sleep quality and overall emotional and physical well-being were assessed using daily sleep diaries and questionnaires which scored the readiness to train, stress levels, fatigue, muscle soreness and flu symptoms respectively. Quality of sleep and subsequent association with the consecutive elements of the well-being questionnaire were assessed through statistical using the student t-test and clinical differences with the methodology of Osoba and colleagues: <5% "no change", 5%-10% "little change"; 10%-20% "moderate change"; and >20% "very much change". Results: The results demonstrated that the professional athletes assessed their sleep quality as average with a mean score of 3.4 out of 5. Lower perceived quality of sleep (<75th percentile) was correlated with a decreased readiness to train (mean score 3.2 [IQR: 3.0-4.0] vs. 3.5 [IQR: 3.0-5.0]; P < 0.001) and increased extent of muscle soreness (2.7 [IQR: 2.0-3.0] vs. 2.3 [IQR: 2-3]; P < 0.001), stress level (mean score 2.3 [IQR: 2.0-3.0] vs. 1.9 [IQR: 1.0-2.0]; P < 0.001) and perceived fatigue (mean score 2.9 [IQR: 2.0-3.0] vs. 2.3 [IQR: 2.0-3.0]; P < 0.001). Likewise, in patients with lower perceived quality of sleep, the proportion of players presenting with flu symptoms increased over 4-fold (4.1% vs. 17%; P < 0.001). Conclusions: This study highlights that good sleep quality positively influences the overall emotional and physical well-being of professional athletes. Our results emphasize the importance of targeted sleep interventions to improve sleep quality and subsequently optimize psychological and physiological wellness.

The effect of fissure morphology and eruption time on penetration and adaptation of pit and fissure sealants: An SEM study.

UNLABELLED: This study was designed to examine the effect of fissure morphology on penetration and adaptation of fissure sealants and their relationship with the eruption time of tooth. MATERIALS AND METHODS: One hundred and fifty extracted molars and premolars were divided into two groups on the basis of their eruption time. The two groups were further divided into five subgroups on the basis of fissure morphology. An scanning electron microscopic analysis of penetration and adaptation of sealant was done. OBSERVATIONS AND RESULTS: V- and U-shaped fissures were found to have the maximum penetration. Penetration was very poor for I- and IK-types of fissures. No significant difference in penetration was found in relation to eruption time. Adaptation of sealant was not affected by any of the factors. CONCLUSION: Even the well-applied sealant does not necessarily provide complete obturation of pits and fissures, thus necessitating periodical clinical observation to determine the success or potential failure of the sealant treatment.

Comparative in vivo evaluation of restoring severely mutilated primary anterior teeth with biological post and crown preparation and reinforced composite restoration.

BACKGROUND: This study was designed to compare the success rate of biological and composite restorations when used to replace structural loss of primary anterior teeth using intracanal post for radicular support of the restoration. MATERIALS AND METHODS: Forty-two patients aged between 3-5 years presenting with early childhood caries (ECC) received at least one or more composite and biological restorations for comparative evaluation. A total of 150 restorations were done (75 biological restorations and 75 composite restorations). The restorations were evaluated single-blind according to a modified USPHS system. Assessment of the patient's response in accepting a biological restoration, psychological impact of the restorations, view of the parents, and peer group reviews, etc. were recorded in a response sheet in presence of the child and the parents. OBSERVATIONS AND RESULTS: In vivo clinical performance of biological post and crown restorations and intracanal reinforced composite restorations was comparable with respect to shade match, marginal discoloration, marginal integrity, surface finish, gingival health, retention, and recurrent carious lesions. The cost effectiveness of biological restorations was certainly a positive attribute. CONCLUSION: The biological restoration presented as a cost effective, clinician friendly, less-technique sensitive, and esthetic alternative to commercially available restorative materials used for restoring deciduous teeth affected by ECC.

A gene encoding a protein with seven zinc finger domains acts on the sexual differentiation pathways of Schizosaccharomyces pombe.

Byr3 was selected as a multicopy suppressor of the sporulation defects of diploid Schizosaccharomyces pombe cells that lack ras1. Like cells mutant at byr1 and byr2, two genes that encode putative protein kinases and that in multiple copies are also suppressors of the sporulation defects of ras1 null diploid cells, cells mutant at byr3 are viable but defective in conjugation. Nucleic acid sequence indicates byr3 has the capacity to encode a protein with seven zinc finger binding domains, similar in structure to the cellular nucleic acid binding protein (CNBP), a human protein that was identified on the basis of its ability to bind DNA. Expression of CNBP in yeast can partially suppress conjugation defects of cells lacking byr3.

D-Aspartate oxidase and D-amino acid oxidase are localised in the peroxisomes of terrestrial gastropods.

D-Aspartate oxidase and D-amino acid oxidase were found in high activity in the tissues of representative species of terrestrial gastropods. Analytical subcellular fractionation demonstrated that both of these oxidases co-localised with the peroxisome markers, acyl-CoA oxidase and catalase, in the digestive gland homogenate. Electron microscopy of peak peroxisome fractions showed particles of uniform size with generally well preserved variably electron-dense matrices bounded by an apparently single limiting membrane. Many of the particles exhibited a core region of enhanced electron density. Catalase cytochemistry of peak fractions confirmed the peroxisome identity of the organelles. Peroxisome-enriched subcellular fractions were used to investigate the properties of gastropod D-aspartate oxidase and D-amino acid oxidase activities. The substrate and inhibitor specificities of the two activities demonstrated that two distinct enzymes were present analogous to, but not identical to, the equivalent mammalian peroxisomal enzymes.

The antigenic domain of flagellin from S. paratyphi shares a structural fold with subtilisin.

Bacterial flagellin has two domains: the polymerizing domain consisting of N- and C-terminal regions which are partly disordered in the monomeric state; and the central antigenic domain with compact globular structure. The polymerizing domain is highly conserved in flagellins from different species but the antigenic domain is diverse in sequence and size. Whereas the former has direct functional significance for bacterial motility, the latter has not been identified as having a specific function except for defining the distinct serotype of the bacterium. The sequence alignment of flagellin from S. paratyphi with proteins of known three-dimensional structure reveals significant homology of the central 265 residue stretch with the bacterial serine protease, subtilisin. This homology is evident also in the comparison of the predicted secondary structure of flagellin with the observed secondary structural features in subtilisin. The deletions/insertions arising due to optimal alignment of the two proteins occur on the surface loops in the structure. Thus, a domain of S. paratyphi flagellin and subtilisin appear to have similar structural folds.

Gastropod mollusc aliphatic alcohol oxidase: subcellular localisation and properties.

The digestive gland and other tissues of several species of terrestrial gastropod mollusc contain an aliphatic alcohol oxidase activity (EC1.1.3.13). The enzyme is FAD dependent, consumes oxygen and generates hydrogen peroxide and the corresponding aldehyde. Saturated primary alcohols are favoured as substrates with octanol preferred with an apparent Km of 3-4 microM. The activity is clearly distinguishable from previously reported molluscan aromatic alcohol oxidase (EC1.1.3.7) on the basis of FAD dependence, sensitivity to heat treatment and high salt concentration and with regard to substrate preferences. The aliphatic alcohol oxidase is membrane associated and most likely localised to the endoplasmic reticulum. Extraction of membranes with 1% Igipal solubilises the enzyme in active form. This enzyme is a further example of an oxidase apparently restricted to molluscs.

Implementation of behaviour management techniques--how well accepted they are today.

Dynamic changes in society with working parents and smaller nuclear families have brought about a sea of changes in parental attitudes and anxieties towards their children. This has affected the perception and implementation of behaviour modification techniques by the dentist. Today, the significance of Tell Show Do technique and its efficiency in behaviour modification is questionable. Insistence of parents on conscious sedation is seen to be on the rise. This study aimed to assess the change in the utilization pattern of behaviour management techniques by pediatric dentists of Punjab in the past five years along with identification of change in parental attitude towards these techniques.

A study of toothbrush contamination at different time intervals and comparative effectiveness of various disinfecting solutions in reducing toothbrush contamination.

Toothbrush contamination during the procedure of brushing was assessed at different time intervals of usage. A peak value of 100% contamination was found at 1 month interval. Hence, a method suggesting disinfection of the toothbrush was employed by comparing the effectiveness of various disinfecting solutions available in the market so that, the brush does not become a source of potential pathogens.

Relationship of tongue-thrust swallowing and anterior open bite with articulation disorders: a clinical study.

A Paediatric dentist may be the first person consulted for professional advice concerning children with speech problems. A positive significant relationship has been hypothesized by some authors between tongue-thrust swallowing and articulation disorders in children. This study was undertaken to find out whether any articulation disorders are associated with the habit of tongue-thrust swallowing, or is it the type of anterior bite that plays an important role in the normal or abnormal speech production in tongue-thrust swallowers. Forty subjects with tongue-thrust swallowing in the age group of 7-16 years were examined. Of these 20 had normal anterior bite while rest had anterior open bite. A word articulation test was used and word level articulation testing was done for initial, medial and final positions. The sounds tested were: Linguoalveolars, Labiodentals, Linguodentals, Linguopalatals, Bilabials and Linguovelars. The results of the study have indicated that the presence of articulation disorders is strongly associated with the anterior open bite present in tongue-thrust swallowers but a simple, direct relationship between the presence of defective consonant sounds and tongue-thrust swallowing has not been found.

Retentive [correction of Preventive] efficacy of glass ionomer, zinc phosphate and zinc polycarboxylate luting cements in preformed stainless steel crowns: a comparative clinical study.

This study was undertaken to assess the efficacy of three luting cements, namely, glass ionomer, zinc phosphate and zinc polycarboxylate in retainng the preformed stainless steel crowns in-vivo. Twenty subjects, with an indication for restoration of three primary molars with stainless steel crowns, were selected. Sixty teeth were taken up for the study, and twenty crowns were cemented with each of the three luting cements. After an eight month follow up the crowns were assessed for their presence/ absence or "rocking". Statistical analysis was done using Chi-square test. The results have shown no significant difference in retentivity of stainless steel crowns with the use of either of the three luting agents.

A comparative study of microleakage below cemento-enamel junction using light cure and chemically cured glass ionomer cement liners.

Posterior composite resins have gained considerable importance over last few years. However, improper placement techniques continue to be a prime cause of failure of these restorations. This study was done to compare the microleakage at the tooth restoration interface extending below cementoenamel junction when posterior composite restorations were placed directly in the cavity or when placed over a glass ionomer cement liner, both chemically cured and light cured. The results have shown that light cured glass ionomer when used as a liner under a composite restoration used below gingival cementum/dentin margin has the best sealing capability.

A comparative study of effects of mouth breathing and normal breathing on gingival health in children.

The present study was conducted to assess the effects of mouth breathing, lip seal and upper lip coverage on gingival health of children. 240 school children aged 10-14 years were selected irrespective of sex race and socioeconomic status. They were divided into two major groups i.e. mouth breathers and normal breathers. These groups were further subdivided into six sub-groups or categories on the basis of lip seal and upper incisor coverage. Gingival index was found to be higher in the mouth breathers than in the normal breathers in the subjects with incompetent lip seal. Increased lip separation and decreased upper lip coverage were all associated with higher levels of Plaque index and Gingival index. No statistical difference existed between mouth breathers and normal breathers with respect to Plaque index.

Comparison of sealing efficacy of materials placed in lateral root perforations--an in vitro study.

An endodontic perforation interferes with the principal goal of sealing the root canal system. An in vitro study was conducted on comparative efficacy of three materials-Dispersalloy, Cavit and Prisma VLC Dycal when placed in lateral root perforations in cervical third areas. Root canals were prepared and irrigated thoroughly. Perforations were made with No. 2 round bur and sealed with Dispersalloy, Cavit and Prisma VLC Dycal. Dispersalloy Group I showed best sealing ability. Prisma VLC Dycal Group I too was comparable to Dispersalloy Group I in its sealing ability, followed by Cavit Group II, Prisma VLC Dycal Group II, Dispersalloy Group II in that order. Cavit Group I was least effective in sealing perforations.