RESUMEN
Medicinal leech therapy is commonly used to treat venous congestion by local application to skin flaps, which are frequently employed in re-construction surgery. In the present study, we investigated the effect of natural hirudin, which was applied locally to treat the venous congestion, in random skin flaps in a Guangxi Bama miniature pig model. The congestive random skin flaps were divided into 3 groups: Group A (control group that was locally applied with saline), Group B (locally applied with 20ATU natural hirudin per each flap), Group C (locally applied with 40ATU natural hirudin per each flap). The microcirculatory changes were observed by measuring myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD). The level of MPO, MDA and SOD activities in the tissue in hirudin-treated groups was significantly different compared with control group (P < 0.05), but there was no significant difference between group B and group C. At 12 days, as compared to the control group, flap survival rate in hirudin-treated groups was significantly different (P < 0.05), but there was no significant difference between the groups receiving two different doses of hirudin. Natural hirudin can improve microcirculation by inhibiting oxygen free radical damage and also improve flap survival time.
Asunto(s)
Antitrombinas/farmacología , Hirudinas/farmacología , Microcirculación/efectos de los fármacos , Colgajos Quirúrgicos/irrigación sanguínea , Animales , Radicales Libres/metabolismo , Supervivencia de Injerto/efectos de los fármacos , Malondialdehído/metabolismo , Peroxidasa/metabolismo , Piel/irrigación sanguínea , Trasplante de Piel , Superóxido Dismutasa/metabolismo , Colgajos Quirúrgicos/patología , PorcinosRESUMEN
We have assessed the anti-inflammatory, anti-oxidative and anti-coagulant effects of locally applied natural and recombinant hirudin in a random skin flap rat model. Thirty Wistar rats with venous congested skin flaps were randomly divided into two treatment groups and a control group to receive subcutaneous injections of natural hirudin (6 U), recombinant hirudin (6 U) or physiological saline, respectively. Superoxide dismutase, malondialdehyde and endothelin levels as well as flap survival rates of the skin flaps were measured after surgery. Compared to the control group, the treatment groups had significant higher superoxide dismutase levels and lower malondialdehyde and endothelin levels in the skin flaps. The surviving areas of the flaps were larger in the treatment groups than the control group. Our results demonstrated that hirudin could improve skin flap survival through its anti-inflammatory, anti-oxidative and anti-coagulant activities.
Asunto(s)
Endotelinas/metabolismo , Hirudinas/farmacología , Malondialdehído/metabolismo , Superóxido Dismutasa/metabolismo , Colgajos Quirúrgicos/irrigación sanguínea , Análisis de Varianza , Animales , Supervivencia de Injerto , Ratas , Ratas WistarRESUMEN
<p><b>OBJECTIVE</b>To detect the expression of TRAIL protein and mRNA in hemangiomas and vascular malformations.</p><p><b>METHODS</b>Sections of 33 proliferative hemangiomas,28 involuting hemangiomas and 29 vascular malformations were immunostained for TRAIL protein, TRAIL mRNA was examined by in situ hybridization in these tissue.</p><p><b>RESULTS</b>The TRAIL protein positive rates in proliferative hemangiomas, involuting hemangiomas, vascular malformations and normal skins were respectively 45.45% (15/33), 78.57% (22/28), 0% and 0%. There were significant differences among the four pathologies (P < 0.01). The difference between proliferative hemangiomas and involuting hemangiomas was also significant (P < 0.01). The TRAIL mRNA positive rates were 66.67% (11/33), 89.29 (25/28), 0% and 0% respectively. There were also significant differences among the four pathologies (P < 0.01). The difference between proliferative hemangiomas and involuting hemangiomas was also significant (P < 0.01).</p><p><b>CONCLUSIONS</b>TRAIL could induce endothelial apoptosis and cause regression of hemangiomas.</p>
Asunto(s)
Femenino , Humanos , Recién Nacido , Masculino , Apoptosis , Hemangioma , Metabolismo , Patología , Hibridación in Situ , Microcirculación , ARN Mensajero , Genética , Ligando Inductor de Apoptosis Relacionado con TNF , Genética , Metabolismo , Malformaciones Vasculares , Metabolismo , PatologíaRESUMEN
<p><b>UNLABELLED</b>OBJECTIVE; To evaluate a technique with retained copper needles for the treatment of venous malformations.</p><p><b>METHODS</b>With 78 venous malformation cases, there were three methods were applied for the treatment respectively, including copper needles in the lesion only, vascular ligation with the copper needles in the lesion, and electrical puncture with the copper needles in the lesion.</p><p><b>RESULTS</b>There were totally 96% effective rate achieved in this clinical data.</p><p><b>CONCLUSIONS</b>The retained copper needles technique may be a simple and effective method for the treatment of venous, malformations resulting in vessel denaturation, fibrosis and disappearance of structure.</p>