Insights into the biogeochemical transformation, environmental impacts and biochar-based soil decontamination of antimony.

Every year, a significant amount of antimony (Sb) enters the environment from natural and anthropogenic sources like mining, smelting, industrial operations, ore processing, vehicle emissions, shooting activities, and coal power plants. Humans, plants, animals, and aquatic life are heavily exposed to hazardous Sb or antimonide by either direct consumption or indirect exposure to Sb in the environment. This review summarizes the current knowledge about Sb global occurrence, its fate, distribution, speciation, associated health hazards, and advanced biochar composites studies used for the remediation of soil contaminated with Sb to lessen Sb bioavailability and toxicity in soil. Anionic metal(loid) like Sb in the soil is significantly immobilized by pristine biochar and its composites, reducing their bioavailability. However, a comprehensive review of the impacts of biochar-based composites on soil Sb remediation is needed. Therefore, the current review focuses on (1) the fundamental aspects of Sb global occurrence, global soil Sb contamination, its transformation in soil, and associated health hazards, (2) the role of different biochar-based composites in the immobilization of Sb from soil to increase biochar applicability toward Sb decontamination. The review aids in developing advanced, efficient, and effective engineered biochar composites for Sb remediation by evaluating novel materials and techniques and through sustainable management of Sb-contaminated soil, ultimately reducing its environmental and health risks.

Synergistic interactions and reaction mechanisms of biochar surface functionalities in antibiotics removal from industrial wastewater.

Biochar, a carbon-rich material with a unique surface chemistry (high abundance of surface functional groups, large surface area, and well-distributed), has shown great potential as a sustainable solution for industrial wastewater treatment as compared to conventional industrial wastewater treatment techniques demand substantial energy consumption and generate detrimental byproducts. This critical review emphasizes the surface functionalities formation and development in biochar to enhance its physiochemical properties, for utilization in antibiotics removal. Factors affecting the formation of functionalities, including carbonization processes, feedstock materials, operating parameters, and the influence of pre-post treatments, are thoroughly highlighted to understand the crucial role of factors influencing biochar properties for optimal antibiotics removal. Furthermore, the research explores the removal mechanisms and interactions of biochar-based surface functionalities, hydrogen bonding, encompassing electrostatic interactions, hydrophobic interactions, π-π interactions, and electron donor and acceptor interactions, to provide insights into the adsorption/removal behavior of antibiotics on biochar surfaces. The review also explains the mechanism of factors influencing the removal of antibiotics in industrial wastewater treatment, including particle size and pore structure, nature and types of surface functional groups, pH and surface charge, temperature, surface modification strategies, hydrophobicity/hydrophilicity, biochar dose, pollutant concentration, contact time, and the presence of coexisting ions and other substances. Finally, the study offers reusability and regeneration, challenges and future perspectives on the development of biochar-based adsorbents and their applications in addressing antibiotics. It concludes by summarizing the key findings and emphasizing the significance of biochar as a sustainable and effective solution for mitigating antibiotics contamination in industrial wastewater.

Biological Control of Chili Damping-Off Disease, Caused by Pythium myriotylum.

Pythium myriotylum is a notorious soil-borne oomycete that causes post-emergence damping-off in chili pepper. Of various disease management strategies, utilization of plant growth promoting rhizobacteria (PGPR) in disease suppression and plant growth promotion is an interesting strategy. The present study was performed to isolate and characterize PGPR indigenous to the chili rhizosphere in Pakistan, and to test the potential to suppress the damping-off and plant growth promotion in chili. Out of a total of 28 antagonists, eight bacterial isolates (4a2, JHL-8, JHL-12, 1C2, RH-24, 1D, 5C, and RH-87) significantly suppressed the colony growth of P. myriotylum in a dual culture experiment. All the tested bacterial isolates were characterized for biochemical attributes, and 16S rRNA sequence based phylogenetic analysis identified these isolates as Flavobacterium spp., Bacillus megaterium, Pseudomonas putida, Bacillus cereus, and Pseudomonas libanensis. All the tested bacterial isolates showed positive test results for ammonia production, starch hydrolase (except 4a2), and hydrogen cyanide production (except 4a2 and 1D). All the tested antagonists produced indole-3-acetic acid (13.4-39.0 µg mL-1), solubilized inorganic phosphate (75-103 µg mL-1), and produced siderophores (17.1-23.7%) in vitro. All the tested bacterial isolates showed varying levels of susceptibility and resistance response against different antibiotics and all these bacterial isolates were found to be non-pathogenic to chili seeds and notably enhanced percentage seed germination, plumule, redical length, and vigor index over un-inoculated control. Additionally, under pathogen pressure, bacterization increased the defense related enzymes such as Peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) activates. Moreover, the treatment of chili seeds with these bacterial isolates significantly suppressed the damping-off caused by P. myriotylum and improved PGP traits compared to the control. In addition, a positive correlation was noticed between shoot, root length, and dry shoot and root weight, and there was a negative correlation between dry shoot, root weight, and seedling percentage mortality. These results showed that native PGPR possesses multiple traits beneficial to the chili plants and can be used to develop eco-friendly and effective seed treatment formulation as an alternative to synthetic chemical fungicides.