RESUMEN
OBJECTIVE: To clarify the expression and clinical significance of metastasis-associated in colon cancer 1 (MACC1) mRNA in hepatocellular carcinoma (HCC). METHODS: The expression and distribution of MACC1 were assessed by quantitative real-time polymerase chain reaction (RT-PCR) and immunohistochemical staining (IHC) in a cohort of hepatitis B virus-related HCC, including 138 in early (A), 96 in intermediate (B) and 120 in advanced stages (C). The association of MACC1 mRNA with disease progression and outcomes was analyzed by univariate and multivariate Cox analysis. RESULTS: The intratumoral expressions of MACC1 mRNA in HCC stage I (0.001 76, range: 0.000 54 - 0.002 47), stage II (0.002 49, range: 0.000 55 - 0.006 78) and stage III (0.008 35, range: 0.006 86 - 0.009 88) were about 3-, 4- and 14-fold higher than that in the normal liver tissue (0.000 59, range: 0.000 57 - 0.000 60), respectively. Intratumoral expression of MACC1 mRNA increased with disease progression from stage I to stage III. HCC clinical staging classification, age, portal vein invasion and tumor differentiation were significantly associated with intratumoral high expression of MACC1 mRNA (All P < 0.05). Immunohistochemical staining showed that there was an increased MACC1 expression in cytoplasm of HCC cells and positive nuclear staining in some cases. Increased MACC1 mRNA expression could predict poor outcome and recurrence in stage A and B HCC postoperatively. The median tumor-free survival and total survival of patients with high MACC1 mRNA expression were 34.0 and 40 months, respectively, significantly lower than that in those with low expression (48.0 and 48.0 months) (all P < 0.01). Cox analysis showed that Child-Pugh grading and high expression of MACC1 mRNA were independent predictive factors, and high expression of MACC1 was an independent predictive factor affecting the tumor-free survival. CONCLUSIONS: MACC1 mRNA up-regulation is a feature of disease progression in HCC. MACC1 mRNA expression in the HCC may become an independent predictive factor for recurrence and survival in postoperative HCC patients.
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Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patología , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patología , Factores de Transcripción/metabolismo , Adulto , Anciano , Carcinoma Hepatocelular/virología , ADN Viral/análisis , Supervivencia sin Enfermedad , Femenino , Estudios de Seguimiento , Virus de la Hepatitis B , Humanos , Neoplasias Hepáticas/virología , Masculino , Persona de Mediana Edad , Recurrencia Local de Neoplasia , Estadificación de Neoplasias , Modelos de Riesgos Proporcionales , ARN Mensajero/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Tasa de Supervivencia , Transactivadores , Factores de Transcripción/genética , Regulación hacia Arriba , Adulto JovenRESUMEN
AIM: To investigate the intratumoral expression of metastasis-associated in colon cancer 1 (MACC1) and c-Met and determine their clinical values associated with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). METHODS: A retrospective study admitted three hundred fifty-four patients with HBV-related HCC. The expression and distribution of MACC1 and c-Met were assessed by quantitative real-time polymerase chain reaction and immunohistochemistry staining. Prognostic factors influencing survival, metastasis and recurrence were assessed. RESULTS: Intratumoral MACC1 level was found to be associated with HCC disease progression. Both median tumor-free survival (TFS) and overall survival (OS) were significantly shorter in the postoperative HCC patients with high intratumoral MACC1 expression, as compared to those with low intratumoral MACC1 levels (TFS: 34 mo vs 48.0 mo, P < 0.001; OS: 40 mo vs 48 mo, P < 0.01). Multivariable analysis indicated that high MACC1 expression or co-expression with c-Met were independent predictors for HCC clinic outcome (P < 0.001). CONCLUSION: High intratumoral MACC1 expression can be associated with enhanced tumor progression and poor outcome of HBV-related HCC. MACC1 may serve as a prognostic biomarker for postoperative HCC.
Asunto(s)
Biomarcadores de Tumor/metabolismo , Carcinoma Hepatocelular/metabolismo , Neoplasias Hepáticas/metabolismo , Recurrencia Local de Neoplasia/metabolismo , Factores de Transcripción/metabolismo , Adulto , Anciano , Carcinoma Hepatocelular/patología , Carcinoma Hepatocelular/virología , Progresión de la Enfermedad , Femenino , Hepatitis B Crónica/complicaciones , Humanos , Neoplasias Hepáticas/patología , Neoplasias Hepáticas/virología , Masculino , Persona de Mediana Edad , Proteínas Proto-Oncogénicas c-met/metabolismo , Estudios Retrospectivos , Análisis de Supervivencia , Transactivadores , Adulto JovenRESUMEN
OBJECTIVE: To study the effect of mycorrhizal fungi on the growth and the main chemical composition accumulation of Dendrobium nobile seedlings. METHODS: Seedlings which inoculated mycorrhizal fungus MF23 grew for 18 months, and were sampled regularly. The stem height and the dry weight of plant organs were measured and the content of dendrobine and polysaccharide were determined to investigate the effect of MF23 on the plant growth and the main chemical composition accumulation of stem. RESULTS: After 18 months growth, the stem height and the dry weight of root, stem and leaf of inoculated seedlings were significantly higher than those of the control, increased by 25.34%, 29.28%, 34.64% and 47.12%, respectively. MF23 increased the highest amount of dendrobine and polysaccharide in stem and also prolonged the accumulation process of polysaccharide. After 12 months growth, the content of dendrobine in both the treatment and the control group reached the highest levels, which were 0.59% and 0.51%, respectively. The content of polysaccharide in the treatment and the control group reached the highest levels after 15 (20.05%) and 12 (17.89%) months growth, respectively. CONCLUSION: Mycorrhizal fungus MF23 significantly promotes the growth of D. nobile seedlings, and had a good application prospects on the cultivation and production of D. nobile.