Deterministic processes structure bacterial genetic communities across an urban landscape.

Land-use change is predicted to act as a driver of zoonotic disease emergence through human exposure to novel microbial diversity, but evidence for the effects of environmental change on microbial communities in vertebrates is lacking. We sample wild birds at 99 wildlife-livestock-human interfaces across Nairobi, Kenya, and use whole genome sequencing to characterise bacterial genes known to be carried on mobile genetic elements (MGEs) within avian-borne Escherichia coli (n = 241). By modelling the diversity of bacterial genes encoding virulence and antimicrobial resistance (AMR) against ecological and anthropogenic forms of urban environmental change, we demonstrate that communities of avian-borne bacterial genes are shaped by the assemblage of co-existing avian, livestock and human communities, and the habitat within which they exist. In showing that non-random processes structure bacterial genetic communities in urban wildlife, these findings suggest that it should be possible to forecast the effects of urban land-use change on microbial diversity.

Biosynthesis and structure of the basement membrane proteoglycan containing heparan sulphate side-chains.

Endothelial, epithelial and muscle cells produce a similar proteoglycan for deposition in basement membrane. This proteoglycan is initially synthesized as a low buoyant density proteoglycan containing 3-5 heparan sulphate side-chains (15,000-65,000 Mr each), along one half of a 400,000 Mr core protein. A portion of the population of these macromolecules is degraded to produce small high density proteoglycans containing a variable-sized core protein less than 100,000 in Mr. This biosynthetic and degradative process probably accounts for the variety of differently sized heparan sulphate proteoglycans that have been isolated from various basement membrane sources.