Enhancing dendritic cell immunotherapy for melanoma using a simple mathematical model.

BACKGROUND: The immunotherapy using dendritic cells (DCs) against different varieties of cancer is an approach that has been previously explored which induces a specific immune response. This work presents a mathematical model of DCs immunotherapy for melanoma in mice based on work by Experimental Immunotherapy Laboratory of the Medicine Faculty in the Universidad Autonoma de Mexico (UNAM). METHOD: The model is a five delay differential equation (DDEs) which represents a simplified view of the immunotherapy mechanisms. The mathematical model takes into account the interactions between tumor cells, dendritic cells, naive cytotoxic T lymphocytes cells (inactivated cytotoxic cells), effector cells (cytotoxic T activated cytotoxic cells) and transforming growth factor ß cytokine (T G F-ß). The model is validated comparing the computer simulation results with biological trial results of the immunotherapy developed by the research group of UNAM. RESULTS: The results of the growth of tumor cells obtained by the control immunotherapy simulation show a similar amount of tumor cell population than the biological data of the control immunotherapy. Moreover, comparing the increase of tumor cells obtained from the immunotherapy simulation and the biological data of the immunotherapy applied by the UNAM researchers obtained errors of approximately 10 %. This allowed us to use the model as a framework to test hypothetical treatments. The numerical simulations suggest that by using more doses of DCs and changing the infusion time, the tumor growth decays compared with the current immunotherapy. In addition, a local sensitivity analysis is performed; the results show that the delay in time " τ", the maximal growth rate of tumor "r" and the maximal efficiency of tumor cytotoxic cells rate "aT" are the most sensitive model parameters. CONCLUSION: By using this mathematical model it is possible to simulate the growth of the tumor cells with or without immunotherapy using the infusion protocol of the UNAM researchers, to obtain a good approximation of the biological trials data. It is worth mentioning that by manipulating the different parameters of the model the effectiveness of the immunotherapy may increase. This last suggests that different protocols could be implemented by the Immunotherapy Laboratory of UNAM in order to improve their results.

Controlling behaviors in couple relationships in the digital age: acceptability of gender violence, sexism, and myths about romantic love / Los comportamientos de control en la pareja en la era digital: la aceptación de la violencia de género, el sexismo y los mitos del amor

Young people have incorporated information and communication technology (ICT) and its influence on socialization as a new instrument to exercise controlling behaviors in their relationships. The present research aims to analyse the influence of some variables that affect social perception of those controlling behaviors, such as the adopted role on the scene (i. e. , protagonist vs. observer) and means of control that is used (i. e. , face-to-face vs. WhatsApp) while considering the effect of attitudinal variables: acceptability of intimate partner violence against women (IPVAW), ambivalent sexism, and myths about romantic love. Two studies were implemented: Study 1 included women (n = 224) and Study 2 included men (n = 120), all of them college students. The main results revealed that both women and men perceive controlling behaviors amongst other peer couples; however, few of them recognize suffering or the exercise of these behaviors within their relationships. In addition, data pointed out the adopted role on the scene and the ideological variables (ambivalent sexism, acceptability of IPVAW, and myths about romantic love) that influenced social perception of dating violence; however, there was no influence of means of control. This research contributes to the previous literature, evidencing that controlling behaviors through technological means are accepted and normalized among young people. Additionally, it shows novel data about young people's social perception of controlling behaviors in their relationships, depending on whether they adopt the role of observer or the role of protagonist in a violent situation

Con las tecnologías de la información y la comunicación (TIC) y su influencia en la socialización, los jóvenes han incorporado un instrumento más para ejercer comportamientos controladores en sus relaciones de pareja. Esta investigación pretende analizar la influencia de algunas variables que afectan a la percepción social de estos comportamientos controladores, como el rol del participante en el escenario (protagonista vs. observador u observadora) y el medio de control utilizado (cara a cara vs. WhatsApp), considerando el efecto de las variables ideológicas: aceptabilidad de la violencia, sexismo y mitos del amor romántico. Se llevaron a cabo dos estudios: un primer estudio con mujeres (n = 224) y uno segundo con hombres (n = 120), todos ellos estudiantes universitarios. Los principales resultados indicaron que tanto mujeres como hombres observan comportamientos controladores en otras parejas de su edad, aunque pocos reconocen sufrir o ejercer estos comportamientos en sus relaciones. Asimismo, se encuentra que el rol que se ocupa en el escenario y las variables ideológicas (sexismo ambivalente, aceptabilidad de la violencia y mitos sobre el amor romántico) influyen en la percepción social de la violencia en la pareja, si bien no se encontró influencia del medio de control. Estos hallazgos constituyen una aportación a la literatura existente, poniendo en evidencia que los comportamientos controladores ejercidos a través de los medios tecnológicos son aceptados y normalizados entre los jóvenes y las jóvenes. Asimismo, proporciona datos novedosos sobre la percepción social que esta población tiene de los comportamientos controladores en las relaciones en función de si se adopta el rol de observador o de protagonista de la situación violenta

ATPase and MHC class II molecules co-expression in Rana pipiens dendritic cells.

Mammalian Langerhans cells are antigen-presenting cells located in different epithelia. These cells have a characteristic ultrastructural pattern, present a plasmatic membrane ATPase activity and constitutively express class II molecules of the major histocompatibility complex. ATPase-positive dendritic cells that are morphologically similar to Langerhans cells have also been found in amphibian epidermis. In order to demonstrate that ATPase-positive dendritic cells of amphibian epidermis express class II molecules and are present in other stratified epithelia, histochemical and immunohistochemical as well as ultrastructural analysis were performed. ATPase-positive dendritic cells and class II-positive dendritic cells were observed in epidermis, nictitant membrane and cornea. In epidermis the number of ATPase-positive dendritic cells was 656+/-186/mm2 while class II-positive dendritic cells was 119+/-45/mm2. Some ATPase-positive dendritic cells showed co-expression of class II molecules. These results suggest the existence of dendritic cell subsets in amphibians as is clearly demonstrated in mammals.

CD11c decrease in mouse thymic dendritic cells after vanadium inhalation.

Vanadium (V) is a transition metal found in air adsorbed onto suspended particles. As a result, urban populations are often exposed to this element as a constituent of particulate matter (PM). One aspect of the myriad toxicities that might arise from these exposures is altered immune responses. Previous reports from the laboratory reported modifications in splenic architecture - with germinal center hyperplasia and a suppressed humoral immune response - in mice that had been exposed to vanadium agents via inhalation. This paper reports a decrease in the presence of the CD11c surface marker on mouse thymic dendritic cells (DC) as a result of host exposure to vanadium (here, in the form of vanadium pentoxide; V(2)O(5)) over a period of 4 weeks. All results were obtained using immunohistochemistry and flow cytometry. It is surmised that this decrease might induce a dysfunction, including possible negative selection of T-cells, which could increase the presence of autoreactive clones in the exposed host. Such an outcome could, in turn, increase the risk for development of autoimmune reactions in different organs specifically, and of autoimmune diseases in general in these V-exposed hosts.

Una Vida entre Rejas: Aspectos Psicosociales de la Encarcelación y Diferencias de Género / A Life behind Bars: Psychosocial Aspect of Incarceration and Gender Differences

Dado el rol que la mujer tiene en la familia, su ingreso en prisión conlleva un estigma familiar,alteración de roles y problemas de apego, efectos que se ven agravados en el caso de tener hijos pequeños en prisión. El propósito de esta investigación es contrastar las diferencias, entre las respuestas que dan hombres y mujeres a una serie de cuestiones referidas a su paso por la cárcel. Concretamente, conocer si el paso por la institución afecta de manera diferencial a unos y otras y analizar las diferencias a la luz de las teorías sobre roles de género. Es por ello que resulta de suma importancia atender el hecho de que no se trata de que las mujeres tengan problemas diferentes a los que pueden hallarse en los hombres, sino que a las consecuencias propias que se derivan del encierro en hombres y mujeres, se suman las propias de la condición de género (AU)

Given the role that women play in the family, their imprisonment brings about a stigma in thefamily, an alteration of roles and attachment problems. These effects worsen for those who have young children while in prison. The purpose of this research is to contrast the differences between men’s and women’s responses to certain issues related to the time they spent in prison. Specifically, the purpose is to analyze whether time in prison affects the genders in different ways and to analyze any differences in the light of gender role theories. It is of utmost importance to address the fact that it is not that women have different problems than those which can be found in men, but rather that the consequences arising from incarceration for men and for women come on top of those differences already inherent to the genders (AU)

Non-specific esterase-positive dendritic cells in epithelia of the frog Rana pipiens.

Langerhans cells are antigen-presenting cells located in epithelia and have a dendritic outline, a convoluted nucleus surrounded by an electron lucent cytoplasm with sparse organelles and occasionally containing the characteristic Birbeck granule; their membrane contains class II molecules of the major histocompatibility complex and a strong membrane reactivity for both ATPase and non-specific esterase. Despite increasing knowledge about mammalian Langerhans cells, only a few studies have examined the possible occurrence of Langerhans-like cells in lower vertebrates. Our group has previously demonstrated the presence of dendritic cells in different epithelial membranes co-expressing a strong membrane ATPase reactivity and class II molecules of the major histocompatibility complex in the frog Rana pipiens. Adding another criterion in the characterization of Langerhans-like cells in amphibians, we now report evidence for the expression of membrane non-specific esterase reactivity in dendritic cells located in the epidermis, nictitant membrane and cornea with topographical and light and electron microscopical characteristics identical to those previously described for dendritic cells positive for ATPase and major histocompatibility complex class II in Rana pipiens. We postulate that, taking all this data together, these dendritic intraepithelial cells constitute the amphibian counterpart of mammalian Langerhans cells.