Screening self-peptides for recognition by mouse alloreactive CD8+ T cells using direct ex vivo multimer staining.

Here, we present a protocol to identify immunogenic self-peptide/allogeneic major histocompatibility complex (MHC) epitopes. We describe the generation of enriched alloreactive CD8+ T cells by priming mice with a skin graft expressing the allogeneic MHC class I molecule of interest, followed by boosting with a liver-specific AAV vector encoding the heavy chain of that donor MHC allomorph. We then use a peptide-exchange approach to assemble a range of peptide-MHC (pMHC) multimers for measuring recognition of the various epitopes by these alloreactive T cells. For complete details on the use and execution of this protocol, please refer to Son et al. (2021).1.

A COVID-19 Risk Assessment for the US Labor Force.

The consequences of COVID-19 infection varies substantially based on individual social risk factors and predisposing health conditions. Understanding this variability may be critical for targeting COVID-19 control measures, resources and policies, including efforts to return people back to the workplace. We compiled individual level data from the National Health Information Survey and Quarterly Census of Earnings and Wages to estimate the number of at-risk workers for each US county and industry, accounting for both social and health risks. Nearly 80% of all workers have at least one health risk and 11% are over 60 with an additional health risk. We document important variation in the at-risk population across states, counties, and industries that could provide a strategic underpinning to a staged return to work.