RESUMEN
We report two cases of encephalopathy (one with seizures, one with electroencephalogram changes) in patients with Zika virus infection. The cases occurred on Martinique in February 2016, during the Zika virus outbreak. Awareness of the various neurological complications of Zika virus infection is needed for patients living in areas affected by Zika virus infections or for travellers to these areas.
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Líquido Cefalorraquídeo/virología , Encefalitis Viral/líquido cefalorraquídeo , Encefalitis Viral/virología , Infección por el Virus Zika/líquido cefalorraquídeo , Infección por el Virus Zika/virología , Virus Zika/aislamiento & purificación , Anciano , Femenino , Humanos , Masculino , Martinica/epidemiología , Vigilancia de la Población , Adulto JovenRESUMEN
We report two cases of Guillain-Barré syndrome who had concomitant Zika virus viruria. This viruria persisted for longer than 15 days after symptom onset. The cases occurred on Martinique in January 2016, at the beginning of the Zika virus outbreak. Awareness of this possible neurological complication of ZikV infection is needed.
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Síndrome de Guillain-Barré/virología , Orina/virología , Infección por el Virus Zika/complicaciones , Virus Zika/aislamiento & purificación , Ensayo de Inmunoadsorción Enzimática , Femenino , Síndrome de Guillain-Barré/complicaciones , Humanos , Masculino , Martinica , Persona de Mediana Edad , ARN Viral/sangre , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Adulto Joven , Virus Zika/genética , Infección por el Virus Zika/diagnósticoRESUMEN
To identify factors associated with disease severity, we examined 102 patients with quantitative PCR-confirmed leptospirosis in Martinique during 2010-2013. Associated factors were hypotension, chest auscultation abnormalities, icterus, oligo/anuria, thrombocytopenia, prothrombin time <68%, high levels of leptospiremia, and infection with L. interrogans serovar Icterohaemorrhagiae/Copenhageni.
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Brotes de Enfermedades , Leptospirosis/epidemiología , Adulto , Animales , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/genética , Enfermedades de los Perros/patología , Perros , Femenino , Humanos , Leptospirosis/sangre , Leptospirosis/genética , Masculino , Martinica/epidemiología , Persona de Mediana Edad , Zoonosis/epidemiologíaRESUMEN
BACKGROUND: Humanitarian aid workers are exposed to deployment-related health threats. Identifying subgroups at a higher risk of infection in this diverse population could help optimize prevention. METHODS: We carried out a retrospective study based on anonymized data of humanitarian aid workers that visited our clinic for a post-deployment visit between 1 January 2018 and 31 December 2021. We conducted a descriptive analysis of basic demographic data, self-reported risk exposure and health problems encountered during deployment extracted from a standard questionnaire. RESULTS: The questionnaire was administered to 1238 aid workers during 1529 post-deployment medical consultations. The median age was 37.2 years (IQR 31.7-44.3), and 718/1529 (47.0%) were female aid workers. The median duration of deployment was 6 months (IQR 3-12 months). Most deployments (1321/1529 (86.4%)) were for a medical organization and in Sub-Saharan Africa (73.2%). The most common risk exposures were contact with freshwater in schistosomiasis endemic regions (187/1308 (14.3%)), unprotected sexual contact with a person other than a regular partner (138/1529 (9.0%)), suspected rabies exposure (56/1529 (3.7%)) and accidental exposure to blood (44/1529 (2.9%)). Gastrointestinal problems (487/1529 (31.9%)), malaria (237/1529 (15.5%)) and respiratory tract infections (94/1529 (6,2%)) were the most encountered health problems. Fifteen volunteers (1%) were hospitalized during deployment and 19 (1.2%) repatriated due to health problems. Adherence to malaria chemoprophylaxis was poor, only taken according to the prescription in 355 out of 1225 (29.0%) of aid workers for whom prophylaxis was indicated. CONCLUSION: Humanitarian aid workers deployed abroad encounter significant rates of health problems and report a high level of risk exposure during their deployment, with the risks being greatest among younger people, those deployed to rural areas, and those working for non-medical organizations. These findings help guide future pre-deployment consultations, to increase awareness and reduce risk behaviour during deployment, as well as focus on adherence to medical advice such as malaria chemoprophylaxis.
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Sistemas de Socorro , Humanos , Femenino , Adulto , Masculino , Estudios Retrospectivos , Encuestas y Cuestionarios , Viaje , Factores de Riesgo , Persona de Mediana EdadRESUMEN
We report the case of a 19-year-old Malian patient, who presented with urethritis and a vesicular rash during the summer of 2022, following a probable heterosexual intercourse. The epidemic context among the male homosexual population and the clinical picture without genital lesions or lymphadenopathy allowed us to discuss both chickenpox and mpox, the latter being finally confirmed by the detection of Monkeypox virus DNA from vesicular fluid.
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Exantema , Mpox , Uretritis , Humanos , Masculino , Adulto Joven , Exantema/etiología , Homosexualidad Masculina , Migrantes , Uretritis/diagnóstico , Uretritis/etiología , Enfermedades Cutáneas Vesiculoampollosas/etiología , Mpox/complicaciones , Mpox/diagnóstico , Mpox/virología , Monkeypox virus/aislamiento & purificaciónRESUMEN
Syphilis reemerged in Martinique in 2004 and initially affected 3 HIV-infected patients. By March 2008, syphilis was diagnosed for 37 men and 18 women. As of October 31, 2009, this outbreak had not yet been brought under control. It initially affected mainly men who had sex with men before it spread to heterosexual persons, minority group members, and crack cocaine users.
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Brotes de Enfermedades , Sífilis/epidemiología , Adulto , Brotes de Enfermedades/prevención & control , Femenino , Infecciones por VIH/complicaciones , Humanos , Masculino , Martinica/epidemiología , Conducta Sexual , Sífilis/complicaciones , Sífilis/prevención & controlRESUMEN
Mycobacterium abscessus is an emerging rapidly growing mycobacterium that causes tuberculous-like lesions in humans. We studied the immune control of this organism in C57BL/6 mice challenged intravenously with 10(7) CFU. Bacteria were eliminated from both the spleen and the liver within 90 days, and liver histology showed organized granulomatous lesions. A T- and B-cell requirement was investigated by challenging Rag2-/-, Cd3epsilon-/-, and muMT-/- mice. Rag2-/- and Cd3epsilon-/- mice were significantly impaired in the ability to clear M. abscessus from the liver and spleen, and muMT-/- mice were significantly impaired in the ability to clear M. abscessus from the liver, suggesting that infection control was primarily T cell dependent in the spleen and both T and B cell dependent in the liver. The liver granulomatous response was similar to that of wild-type controls in muMT-/- mice but completely absent in Cd3epsilon-/- and Rag2-/- mice. We studied the involvement of gamma interferon (IFN-gamma) and tumor necrosis factor (TNF) by challenging C57BL/6 mice deficient in the IFN-gamma receptor (Ifngr1-/-) and in TNF (Tnf-/-). Ifngr1-/- mice were significantly impaired in M. abscessus control both in the spleen and in the liver, and granulomas were profoundly altered. The effect was even more substantial in Tnf-/- mice; they failed to control M. abscessus infection in the liver and died within 20 to 25 days after infection with many hepatic inflammatory foci and major lesions of ischemic necrosis in the liver and kidney. These features were not observed with the closely related species M. chelonae. T-cell immunity, IFN-gamma, and TNF are central factors for the control of M. abscessus in C57BL/6 mice, as they are for the control of pathogenic slowly growing mycobacteria.
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Interferón gamma/inmunología , Infecciones por Mycobacterium/inmunología , Mycobacterium/inmunología , Linfocitos T/inmunología , Factor de Necrosis Tumoral alfa/inmunología , Animales , Linfocitos B/inmunología , Linfocitos B/microbiología , Modelos Animales de Enfermedad , Memoria Inmunológica , Hígado/inmunología , Hígado/microbiología , Hígado/patología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Endogámicos DBA , Ratones Noqueados , Mycobacterium/crecimiento & desarrollo , Infecciones por Mycobacterium/microbiología , Bazo/inmunología , Bazo/microbiología , Linfocitos T/microbiologíaRESUMEN
We conducted an observational study to describe the characteristics of infective endocarditis (IE) in French West Indies (FWI) and to identify variables associated with in-hospital case fatality. The records of the patients admitted for IE to the University Hospital of Martinique between 2000 and 2012 were collected using an electronic case report form. Only Duke-Li definite cases were considered for this analysis. Variables associated with in-hospital mortality were tested using univariate logistic regression analysis. The analysis included 201 patients (median age 58 years, sex ratio: two males to one female). There was no previously known heart disease in 45.8%, a prosthetic valve in 21.4%, and previously known native valve disease in 32.8% of the cases. Community-acquired IE represented 59.7% of all cases, health-care-associated IE represented 38.3% and injection-drug-use-acquired IE represented 1.5%. Locations of IE were distributed as follows: 42.3% were mitral valve IE, 34.8% were aortic valve IE, and 7% were right-sided IE. Microorganisms recovered from blood cultures included 30.4% streptococci, 28.9% staphylococci, and 5% enterococci. Blood cultures were negative in 20.9% of the cases. Surgical treatment was performed in 53% of the patients. In-hospital case fatality rate was 19%. Advanced age, Staphylococcus aureus IE, and health-care-associated IE were associated with in-hospital case fatality. The epidemiological and microbiological profile of IE in FWI is in between those observed in developed countries and developing countries: patients were younger, blood cultures were more frequently negative, and IE due to group D streptococci and enterococci were less common than in industrialized countries.
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Países Desarrollados/estadística & datos numéricos , Países en Desarrollo/estadística & datos numéricos , Endocarditis/epidemiología , Endocarditis/mortalidad , Adulto , Factores de Edad , Femenino , Humanos , Masculino , Martinica/epidemiología , Persona de Mediana Edad , Factores de Riesgo , Indias Occidentales/epidemiologíaRESUMEN
BACKGROUND: Leptospirosis is a potentially life-threatening but curable zoonosis whose prognosis depends on accurate and timely diagnosis. Because of its non-specific clinical presentation, laboratory testing is essential to confirm the diagnosis. Here, we aimed to assess the performance of two enzyme-linked immunosorbent assays (ELISAs) (ELISA Serion and ELISA-Hb Pasteur) and one immunodot (GenBio) using quantitative PCR (qPCR) as gold standard, instead of the traditional microscopic agglutination test, for the diagnosis of acute leptospirosis in an endemic area. METHODOLOGY/PRINCIPAL FINDINGS: Between January 2011 and December 2012, a total of 122 patients were diagnosed with leptospirosis, as confirmed by qPCR at the University Hospital of Martinique. Among them, 103 had at least one serum sample available for analysis. Performance of each serological assay was evaluated according to days' post onset of symptoms (DPO) and local species diversity (which included L. santarosai, L. interrogans, L. kirschneri, L. borgpetersenii, L. noguchii, and L. kmetyi). Several thresholds were tested to optimize accuracy. When considering the manufacturer's threshold, the sensitivity of ELISA Serion, ELISA-Hb Pasteur and GenBio immunodot was 75%, 67% and 64%, while specificity was 92%, 98% and 100%, respectively. Moreover, the threshold optimization allowed a significant improvement in specificity for the ELISA Serion from 92% to 99% (p<0.05). During the first 5 DPO, sensitivities were 35%, 30% and 42% for ELISA Serion, ELISA-Hb Pasteur and GenBio immunodot, respectively. However, between 6â10 DPO, these sensitivities dramatically increased to reach 86%, 76% and 67%, respectively. Performances of the three assays were not affected by the species studied. CONCLUSIONS/SIGNIFICANCE: All these serological assays showed the potential for diagnosing leptospirosis after (but not before) 6 days' post onset of symptoms. In a high prevalence setting, where highest specificities are needed, threshold optimizing should be performed for this purpose.
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Leptospirosis/diagnóstico , Pruebas Serológicas/métodos , Diagnóstico Precoz , Ensayo de Inmunoadsorción Enzimática/métodos , Humanos , Immunoblotting/métodos , Martinica , Sensibilidad y Especificidad , Factores de TiempoRESUMEN
A syphilis outbreak began in Martinique, French Antilles, in 2004, initially among men who had sex with men (MSM) and who were living with human immunodeficiency virus (HIV). The outbreak subsequently affected all groups at risk, leading to a first epidemic peak in 2008. After an initial decrease, the outbreak started growing again in 2014 among patients living with HIV with unprecedented incidence among MSM. Herein, we describe the change in medical and social parameters of the outbreak since 2005.
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Brotes de Enfermedades , Infecciones por VIH/complicaciones , Sífilis/complicaciones , Adulto , Femenino , Infecciones por VIH/epidemiología , Humanos , Masculino , Martinica/epidemiología , Conducta Sexual , Sífilis/epidemiologíaRESUMEN
BACKGROUND: Few on-site studies involving local doctors have been published. METHODS: We conducted a prospective on-site study of health problems occurring among French tourists to Nepal between 1 January 2001 and 31 December 2001, and compared the results with those of an identical study performed in 1984. RESULTS: Of the 21,457 French tourists who visited Nepal in 2001, 276 (1.3%) consulted the French Embassy doctor in Kathmandu with health complaints. The main reasons for seeking medical advice were diarrhea (26.8%), high-altitude illness (15.6%), lower respiratory tract infections (11.6%), dermatoses (8.7%), and fever (8.7%). Fifteen patients (5.4%) required hospitalization, five required medical evacuation (1.8%), and 14 (5%) were rescued by helicopter in the Himalayas. One patient died of cardiovascular disease. Relative to the 1984 cohort, significantly more patients consulted for high-altitude illness (p<.001), lower respiratory tract infections (p=.001), physical trauma (p=.01), and psychiatric disorders (p<.001), and significantly fewer patients consulted for dermatoses (p=.04), sexually transmitted diseases (p=.001), and upper respiratory tract infections (p=.005). CONCLUSION: These results, obtained 17 years apart, illustrate the changes in the pattern of health disorders causing travelers in Nepal to consult a doctor.
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Mal de Altura/epidemiología , Encuestas Epidemiológicas , Montañismo , Aceptación de la Atención de Salud/estadística & datos numéricos , Viaje , Mal de Altura/etiología , Estudios de Cohortes , Diarrea/epidemiología , Diarrea/etiología , Fiebre/epidemiología , Fiebre/etiología , Francia/etnología , Humanos , Nepal/epidemiología , Estudios Prospectivos , Infecciones del Sistema Respiratorio/epidemiología , Infecciones del Sistema Respiratorio/etiología , Enfermedades de la Piel/epidemiología , Enfermedades de la Piel/etiologíaRESUMEN
Few data related to the care of children injured in an earthquake are available. The objective of our study was to analyze the results of clinical and microbiologic characteristics of children treated in a Fort de France hospital after the Haiti earthquake. Bacteria were mainly Gram-negative bacteria. Some of these infections seemed to be environmental; a significant portion was related to acquisition during hospitalization.
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Desastres/estadística & datos numéricos , Terremotos , Heridas y Lesiones/microbiología , Infecciones Bacterianas/microbiología , Sangre/microbiología , Catéteres/microbiología , Niño , Femenino , Haití/epidemiología , Hospitales Universitarios , Humanos , Masculino , Micosis/microbiología , Supuración/microbiología , Heridas y Lesiones/epidemiologíaRESUMEN
Shewanella spp. are saprophytic bacteria that are part of the marine microflora in warm climates and are rarely pathogenic. However, Shewanella spp. infections are being increasingly reported, and there has been no comprehensive review of the literature describing these infections. This article reports 16 cases of Shewanella spp. infections in Martinique since 1997 and reviews another 239 cases reported in the literature since 1973. Patients experienced soft tissue infections, ear infection, or abdominal and biliary tract infections. A skin or mucosal portal of entry was found for 53% of the patients and exposure to the marine environment was reported for 44%; 79% of patients had an underlying condition. Bacteriema were frequent (28%). Most (87%) patients recovered, although ear infections can become chronic. Death occurred in 13% of the patients. Most Shewanella spp. isolates are susceptible to cefotaxime (95%), piperacillin and tazobactam (98%), gentamicin (99%), and ciprofloxacin (94%).
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Infecciones por Bacterias Gramnegativas/epidemiología , Shewanella putrefaciens , Shewanella , Adulto , Anciano , Anciano de 80 o más Años , Antibacterianos/uso terapéutico , Enfermedades de las Vías Biliares/tratamiento farmacológico , Enfermedades de las Vías Biliares/epidemiología , Enfermedades de las Vías Biliares/microbiología , Femenino , Infecciones por Bacterias Gramnegativas/tratamiento farmacológico , Humanos , Recién Nacido , Masculino , Martinica/epidemiología , Persona de Mediana Edad , Otitis/tratamiento farmacológico , Otitis/epidemiología , Otitis/microbiología , Infecciones de los Tejidos Blandos/tratamiento farmacológico , Infecciones de los Tejidos Blandos/epidemiología , Infecciones de los Tejidos Blandos/microbiologíaRESUMEN
OBJECTIVE: Characterization of HIV-1 sequences in newly infected individuals is important for elucidating the mechanisms of viral sexual transmission. We report the identification of transmitted/founder viruses in eight pairs of HIV-1 sexually-infected patients enrolled at the time of primary infection ("recipients") and their transmitting partners ("donors"). METHODS: Using a single genome-amplification approach, we compared quasispecies in donors and recipients on the basis of 316 and 376 C2V5 env sequences amplified from plasma viral RNA and PBMC-associated DNA, respectively. RESULTS: Both DNA and RNA sequences indicated very homogeneous viral populations in all recipients, suggesting transmission of a single variant, even in cases of recent sexually transmitted infections (STIs) in donors (nâ=â2) or recipients (nâ=â3). In all pairs, the transmitted/founder virus was derived from an infrequent variant population within the blood of the donor. The donor variant sequences most closely related to the recipient sequences were found in plasma samples in 3/8 cases and/or in PBMC samples in 6/8 cases. Although donors were exclusively (nâ=â4) or predominantly (nâ=â4) infected by CCR5-tropic (R5) strains, two recipients were infected with highly homogeneous CXCR4/dual-mixed-tropic (X4/DM) viral populations, identified in both DNA and RNA. The proportion of X4/DM quasispecies in donors was higher in cases of X4/DM than R5 HIV transmission (16.7-22.0% versus 0-2.6%), suggesting that X4/DM transmission may be associated with a threshold population of X4/DM circulating quasispecies in donors. CONCLUSIONS: These suggest that a severe genetic bottleneck occurs during subtype B HIV-1 heterosexual and homosexual transmission. Sexually-transmitted/founder virus cannot be directly predicted by analysis of the donor's quasispecies in plasma and/or PBMC. Additional studies are required to fully understand the traits that confer the capacity to transmit and establish infection, and determine the role of concomitant STIs in mitigating the genetic bottleneck in mucosal HIV transmission.
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Infecciones por VIH/transmisión , Infecciones por VIH/virología , VIH-1/fisiología , Leucocitos Mononucleares/virología , Parejas Sexuales , Carga Viral , Adulto , Evolución Molecular , Femenino , Variación Genética , Genotipo , Proteína gp120 de Envoltorio del VIH/genética , VIH-1/clasificación , Humanos , Masculino , Datos de Secuencia Molecular , Filogenia , ARN Viral/genética , Tropismo Viral , Adulto JovenRESUMEN
BACKGROUND: Leptospirosis is one of the most important neglected tropical bacterial diseases in Latin America and the Caribbean. However, very little is known about the circulating etiological agents of leptospirosis in this region. In this study, we describe the serological and molecular features of leptospires isolated from 104 leptospirosis patients in Guadeloupe (nâ=â85) and Martinique (nâ=â19) and six rats captured in Guadeloupe, between 2004 and 2012. METHODS AND FINDINGS: Strains were studied by serogrouping, PFGE, MLVA, and sequencing 16SrRNA and secY. DNA extracts from blood samples collected from 36 patients in Martinique were also used for molecular typing of leptospires via PCR. Phylogenetic analyses revealed thirteen different genotypes clustered into five main clades that corresponded to the species: L. interrogans, L. kirschneri, L. borgpetersenii, L. noguchi, and L. santarosai. We also identified L. kmetyi in at least two patients with acute leptospirosis. This is the first time, to our knowledge, that this species has been identified in humans. The most prevalent genotypes were associated with L. interrogans serovars Icterohaemorrhagiae and Copenhageni, L. kirschneri serovar Bogvere, and L. borgpetersenii serovar Arborea. We were unable to identify nine strains at the serovar level and comparison of genotyping results to the MLST database revealed new secY alleles. CONCLUSIONS: The overall serovar distribution in the French West Indies was unique compared to the neighboring islands. Typing of leptospiral isolates also suggested the existence of previously undescribed serovars.
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Leptospira interrogans/clasificación , Leptospirosis/epidemiología , Leptospirosis/microbiología , Leptospirosis/veterinaria , Animales , Análisis por Conglomerados , ADN Bacteriano/química , ADN Bacteriano/genética , Genotipo , Guadalupe/epidemiología , Humanos , Leptospira interrogans/genética , Leptospira interrogans/inmunología , Leptospira interrogans/aislamiento & purificación , Martinica/epidemiología , Datos de Secuencia Molecular , Tipificación Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Ratas , Análisis de Secuencia de ADN , SerotipificaciónAsunto(s)
Infecciones por Uncinaria/diagnóstico , Infecciones por Uncinaria/tratamiento farmacológico , Larva Migrans/diagnóstico , Larva Migrans/tratamiento farmacológico , Viaje , Albendazol/uso terapéutico , Ancylostomatoidea/aislamiento & purificación , Animales , Antihelmínticos/uso terapéutico , Diagnóstico Diferencial , Infecciones por Uncinaria/parasitología , Humanos , Larva Migrans/parasitología , Clima TropicalRESUMEN
Three athletes who participated in a race in the tropical forest of the Caribbean island of Martinique were subsequently diagnosed with leptospirosis using polymerase chain reaction (PCR). We investigated an outbreak to evaluate possible risk factors, and to determine the appropriate public health recommendations. Of 230 athletes, we contacted 148 (64%) and 20 (13.5%) met our case definition. Five were hospitalized and none were fatal. Ten (91%) of the 11 ill athletes who were tested were confirmed by PCR or serology. Serogroup Pyrogenes was commonly found. Cutaneous cuts, reported by 14 (73.7%), was the only potential risk factor using univariate analysis. Sporting event participants in tropical areas should be made aware of specific warnings and recommendations concerning the risk of leptospirosis, especially after periods of heavy rainfall or flooding. Rapid diagnostic assays such as PCR are particularly appropriate in this setting for early diagnosis and for formulating public health recommendations.
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Brotes de Enfermedades , Leptospirosis/epidemiología , Adulto , Femenino , Humanos , Leptospira/clasificación , Masculino , Martinica/epidemiología , Persona de Mediana Edad , Factores de Riesgo , Deportes , ÁrbolesRESUMEN
Aeromonas species are Gram-negative bacilli of the water environment whose survival appears facilitated by warm climates. There have been no reports on Aeromonas species in the [corrected] Caribbean to date. Our aim was to describe clinical and bacteriological features in patients presenting with such bacteremia in Martinique and Guadeloupe. During a 14-year period, we retrospectively identified 37 patients. The mean age was 55 years and in 89% of cases underlying disease such as digestive diseases, cutaneous wounds, and malignancy were identified. One case was related to severe strongyloidiasis and one with snake bite. Polymicrobial bacteremia was identified in 38%, essentially with Enterobacteriaceae. All Aeromonas isolates were resistant to amoxicillin but extended-spectrum beta-lactam and fluoroquinolone were active against more than 95%. During hospitalization 10 patients died (27%). Older age, occurrence of multiorgan failure, and impaired renal function were associated with in-hospital mortality.
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Aeromonas hydrophila/aislamiento & purificación , Bacteriemia/microbiología , Infecciones por Bacterias Gramnegativas/microbiología , Adulto , Anciano , Anciano de 80 o más Años , Bacteriemia/epidemiología , Femenino , Infecciones por Bacterias Gramnegativas/epidemiología , Guadalupe/epidemiología , Humanos , Masculino , Martinica/epidemiología , Persona de Mediana Edad , Factores de Riesgo , Adulto JovenRESUMEN
BACKGROUND: Key symptoms observed during the febrile phase of dengue may identify patients who are likely to progress to severe disease. OBJECTIVES: To test this hypothesis, we examined the relationships between symptoms reported by patients at presentation and the development of severe outcomes. STUDY DESIGN: Retrospective analysis of data recorded prospectively in 560 adult dengue patients admitted to an emergency department. A logistic regression analysis was used to quantify the association between symptoms reported at presentation and outcome. RESULTS: Plasma leakage was observed in 95 patients (17%), severe thrombocytopenia (platelet counts <20 x 10(9)/L) in 93 patients (16.6%) and acute hepatitis in 42 patients (7.5%). Severe thrombocytopenia developed in 57% of patients with plasma leakage and 40.5% of patients with hepatitis. Patients who developed a plasma leakage syndrome were older, mainly male, and reported more often an abdominal pain and a cough. Diarrhea and taking paracetamol >60 mg/kg/day before admission were associated with the development of acute hepatitis. Seven patients died. The mortality rate was 6/95 (6.3%) in patients who developed plasma leakage, 3/42 (7.1%) in patients who developed hepatitis, 5/93 (5.4%) in patients with severe thrombocytopenia, and 3/12 (25%) in the patients who demonstrated together all these severe manifestations. CONCLUSION: Plasma leakage, severe thrombocytopenia and acute hepatitis identified subgroups of adult dengue patients with increased mortality rates. Key symptoms reported by the patients at presentation such as abdominal pain, cough or diarrhea were significantly associated with the development of severe manifestations and should be considered as warning signs.