Determinants of exposure to endocrine disruptors following hurricane Harvey.

Hurricane Harvey was a category four storm that induced catastrophic flooding in the Houston metropolitan area. Following the hurricane there was increased concern regarding chemical exposures due to damage caused by flood waters and emergency excess emissions from industrial facilities. This study utilized personal passive samplers in the form of silicone wristbands in Houston, TX to both assess chemical exposure to endocrine disrupting chemicals (EDCs) immediately after the hurricane and determine participant characteristics associated with higher concentrations of exposure. Participants from the Houston-3H cohort (n = 172) wore a wristband for seven days and completed a questionnaire to determine various flood-related and demographic variables. Bivariate and multivariate analysis indicated that living in an area with a high Area Deprivation Index (ADI) (indicative of low socioeconomic status), identifying as Black/African American or Latino, and living in the Houston neighborhoods of Baytown and East Houston were associated with increased exposure to EDCs. These results provide evidence of racial/ethnic and socioeconomic injustices in exposure to EDCs in the Houston Metropolitan Area. Since the multiple regression models conducted did not fully explain exposure (0.047 < R2 < 0.34), more research is needed on the direct sources of EDCs within this area to create effective exposure mitigation strategies.

Effect of diet energy level and genomic residual feed intake on bred Holstein dairy heifer growth and feed efficiency.

The objective of this study was to determine growth, feed intake, and feed efficiency of postbred dairy heifers with different genomic residual feed intake (RFI) predicted as a lactating cow when offered diets differing in energy density. Postbred Holstein heifers (n = 128, ages 14-20 mo) were blocked by initial weight (high, medium-high, medium-low, and low) with 32 heifers per block. Each weight block was sorted by RFI (high or low) to obtain 2 pens of heifers with high and low genomically predicted RFI within each block (8 heifers per pen). Low RFI heifers were expected to have greater feed efficiency than high RFI heifers. Dietary treatments consisted of a higher energy control diet based on corn silage and alfalfa haylage [HE; 62.7% total digestible nutrients, 11.8% crude protein, and 45.6% neutral detergent fiber; dry matter (DM) basis], and a lower energy diet diluted with straw (LE; 57.0% total digestible nutrients, 11.7% crude protein, and 50.1% neutral detergent fiber; DM basis). Each pen within a block was randomly allocated a diet treatment to obtain a 2 × 2 factorial arrangement (2 RFI levels and 2 dietary energy levels). Diets were offered in a 120-d trial. Dry matter intake by heifers was affected by diet (11.0 vs. 10.0 kg/d for HE and LE, respectively) but not by RFI or the interaction of RFI and diet. Daily gain was affected by the interaction of RFI and diet, with low RFI heifers gaining more than high RFI heifers when fed LE (0.94 vs. 0.85 kg/d for low and high RFI, respectively), but no difference for RFI groups when fed HE (1.16 vs. 1.19 kg/d for low and high RFI, respectively). Respective feed efficiencies were improved for low RFI compared with high RFI heifers when fed LE (10.6 vs. 11.8 kg of feed DM/kg of gain), but no effect of RFI was found when fed HE (9.4 vs. 9.5 kg of DM/kg of gain for high and low RFI, respectively). No effect of RFI or diet on first-lactation performance through 150 DIM was observed. Based on these results, the feed efficiency of heifers having different genomic RFI may be dependent on diet energy level, whereby low RFI heifers utilized the LE diet more efficiently. The higher fiber straw (LE) diet controlled intake and maintained more desirable heifer weight gains. This suggests that selection for improved RFI in lactating cows may improve feed efficiency in growing heifers when fed to meet growth goals of 0.9 to 1.0 kg of gain/d.

Effect of diet energy density and genomic residual feed intake on prebred dairy heifer feed efficiency, growth, and manure excretion.

The objective of this study was to determine the growth, feed efficiency, and manure excretion of prebred dairy heifers with differing predicted genomic residual feed intakes (RFI) when offered diets differing in energy density. Prebred Holstein heifers (n = 128, ages 4 to 8 mo) were blocked by weight (low, medium-low, medium-high, or high) with 32 heifers per block. Heifers in each weight block were grouped by RFI and randomly assigned to obtain 2 pens of high (HRFI) and 2 pens of low RFI (LRFI) heifers within each block (8 heifers/pen). Heifers with LRFI were hypothesized to have greater feed efficiency than HRFI heifers. Dietary treatments were a high-energy diet (HE; 66.6% total digestible nutrients, 14.0% crude protein, and 36.3% neutral detergent fiber, dry matter basis) and a low-energy diet (LE; 63.8% total digestible nutrients, 13.5% crude protein, and 41.2% neutral detergent fiber, dry matter basis). Each pen of heifers was randomly assigned to a treatment to obtain a 2 × 2 factorial arrangement (2 RFI levels × 2 diet energy densities). Diets were offered in a 120-d trial. Dry matter intake was not affected by diet, RFI, or their interaction. Average daily gain (ADG) was affected by diet, with heifers fed HE having greater ADG than heifers fed LE. In addition, RFI affected ADG, with LRFI heifers having greater ADG than HRFI heifers, whereas the interaction of RFI and diet was not significant. Feed efficiency was improved for heifers fed the HE diet, but it was not affected by RFI or the interaction of RFI and diet. Overall, feed efficiency of prebred heifers was not dependent on predicted genomic RFI, because the greater ADG of LRFI heifers was accompanied by slightly higher dry matter intake. Feed efficiency of heifers was reduced when heifers were fed the LE diet, but this resulted in more optimal ADG compared with the HE diet fed for ad libitum intake.

Uncovering the Neoproterozoic carbon cycle.

Interpretations of major climatic and biological events in Earth history are, in large part, derived from the stable carbon isotope records of carbonate rocks and sedimentary organic matter. Neoproterozoic carbonate records contain unusual and large negative isotopic anomalies within long periods (10-100 million years) characterized by δ(13)C in carbonate (δ(13)C(carb)) enriched to more than +5 per mil. Classically, δ(13)C(carb) is interpreted as a metric of the relative fraction of carbon buried as organic matter in marine sediments, which can be linked to oxygen accumulation through the stoichiometry of primary production. If a change in the isotopic composition of marine dissolved inorganic carbon is responsible for these excursions, it is expected that records of δ(13)C(carb) and δ(13)C in organic carbon (δ(13)C(org)) will covary, offset by the fractionation imparted by primary production. The documentation of several Neoproterozoic δ(13)C(carb) excursions that are decoupled from δ(13)C(org), however, indicates that other mechanisms may account for these excursions. Here we present δ(13)C data from Mongolia, northwest Canada and Namibia that capture multiple large-amplitude (over 10 per mil) negative carbon isotope anomalies, and use these data in a new quantitative mixing model to examine the behaviour of the Neoproterozoic carbon cycle. We find that carbonate and organic carbon isotope data from Mongolia and Canada are tightly coupled through multiple δ(13)C(carb) excursions, quantitatively ruling out previously suggested alternative explanations, such as diagenesis or the presence and terminal oxidation of a large marine dissolved organic carbon reservoir. Our data from Namibia, which do not record isotopic covariance, can be explained by simple mixing with a detrital flux of organic matter. We thus interpret δ(13)C(carb) anomalies as recording a primary perturbation to the surface carbon cycle. This interpretation requires the revisiting of models linking drastic isotope excursions to deep ocean oxygenation and the opening of environments capable of supporting animals.

Next-generation sequencing for diagnosis of thoracic aortic aneurysms and dissections: diagnostic yield, novel mutations and genotype phenotype correlations.

BACKGROUND: Thoracic aortic aneurysms and dissections (TAAD) are silent but possibly lethal condition with up to 40 % of cases being hereditary. Genetic background is heterogeneous. Recently next-generation sequencing enabled efficient and cost-effective examination of gene panels. Aim of the study was to define the diagnostic yield of NGS in the 51 TAAD patients and to look for genotype-phenotype correlations within families of the patients with TAAD. METHODS: 51 unrelated TAAD patients were examined by either whole exome sequencing or TruSight One sequencing panel. We analyzed rare variants in 10 established thoracic aortic aneurysms-associated genes. Whenever possible, we looked for co-segregation in the families. Kaplan-Meier survival curve was constructed to compare the event-free survival depending on genotype. Aortic events were defined as acute aortic dissection or first planned aortic surgery. RESULTS AND DISCUSSION: In 21 TAAD patients we found 22 rare variants, 6 (27.3 %) of these were previously reported, and 16 (73.7 %) were novel. Based on segregation data, functional analysis and software estimations we assumed that three of novel variants were causative, nine likely causative. Remaining four were classified as of unknown significance (2) and likely benign (2). In all, 9 (17.6 %) of 51 probands had a positive result when considering variants classified as causative only and 18 (35.3 %) if likely causative were also included. Genotype-positive probands (n = 18) showed shorter mean event free survival (41 years, CI 35-46) than reference group, i.e. those (n = 29) without any plausible variant identified (51 years, CI 45-57, p = 0.0083). This effect was also found when the 'genotype-positive' group was restricted to probands with 'likely causative' variants (p = 0.0092) which further supports pathogenicity of these variants. The mean event free survival was particularly low (37 years, CI 27-47) among the probands with defects in the TGF beta signaling (p = 0.0033 vs. the reference group). CONCLUSIONS: This study broadens the spectrum of genetic background of thoracic aneurysms and dissections and supports its potential role as a prognostic factor in the patients with the disease.

Relationship between processing score and kernel-fraction particle size in whole-plant corn silage.

Kernel processing increases starch digestibility in whole-plant corn silage (WPCS). Corn silage processing score (CSPS), the percentage of starch passing through a 4.75-mm sieve, is widely used to assess degree of kernel breakage in WPCS. However, the geometric mean particle size (GMPS) of the kernel-fraction that passes through the 4.75-mm sieve has not been well described. Therefore, the objectives of this study were (1) to evaluate particle size distribution and digestibility of kernels cut in varied particle sizes; (2) to propose a method to measure GMPS in WPCS kernels; and (3) to evaluate the relationship between CSPS and GMPS of the kernel fraction in WPCS. Composite samples of unfermented, dried kernels from 110 corn hybrids commonly used for silage production were kept whole (WH) or manually cut in 2, 4, 8, 16, 32 or 64 pieces (2P, 4P, 8P, 16P, 32P, and 64P, respectively). Dry sieving to determine GMPS, surface area, and particle size distribution using 9 sieves with nominal square apertures of 9.50, 6.70, 4.75, 3.35, 2.36, 1.70, 1.18, and 0.59 mm and pan, as well as ruminal in situ dry matter (DM) digestibilities were performed for each kernel particle number treatment. Incubation times were 0, 3, 6, 12, and 24 h. The ruminal in situ DM disappearance of unfermented kernels increased with the reduction in particle size of corn kernels. Kernels kept whole had the lowest ruminal DM disappearance for all time points with maximum DM disappearance of 6.9% at 24 h and the greatest disappearance was observed for 64P, followed by 32P and 16P. Samples of WPCS (n=80) from 3 studies representing varied theoretical length of cut settings and processor types and settings were also evaluated. Each WPCS sample was divided in 2 and then dried at 60 °C for 48 h. The CSPS was determined in duplicate on 1 of the split samples, whereas on the other split sample the kernel and stover fractions were separated using a hydrodynamic separation procedure. After separation, the kernel fraction was redried at 60°C for 48 h in a forced-air oven and dry sieved to determine GMPS and surface area. Linear relationships between CSPS from WPCS (n=80) and kernel fraction GMPS, surface area, and proportion passing through the 4.75-mm screen were poor. Strong quadratic relationships between proportion of kernel fraction passing through the 4.75-mm screen and kernel fraction GMPS and surface area were observed. These findings suggest that hydrodynamic separation and dry sieving of the kernel fraction may provide a better assessment of kernel breakage in WPCS than CSPS.

Growth performance and sorting characteristics of corn silage-alfalfa haylage diets with or without forage dilution offered to replacement Holstein dairy heifers.

Gravid heifers consuming high-quality forage diets are susceptible to excessive weight gains and overconditioning. One approach for controlling this problem is to dilute diets with low-energy forages, such as straw, that reduce the caloric density and dry matter intake (DMI) of that diet by heifers. These diluting agents are often sortable by dairy heifers, but previous visual evidence has suggested that eastern gamagrass haylage may be a nonsortable alternative. Our objectives were (1) to compare the growth performance of dairy heifers offered a high-quality forage diet (control) with diets containing 1 of 3 diluting agents [eastern gamagrass haylage (EGH), chopped wheat straw (WS), or chopped corn fodder (CF)]; and (2) evaluate sorting behaviors of heifers offered these forage diets. Holstein heifers (n=128) were stratified (32 heifers/block) on the basis of initial body weight (heavy, 560 ± 27.7 kg; medium-heavy, 481 ± 17.7 kg; medium-light, 441 ± 22.0 kg; and light, 399 ± 14.4 kg), and then assigned to 1 of 16 identical research pens (4 pens/block; 8 heifers/pen), where each of the 4 research diets were assigned to 1 pen within each block. Diets were offered in a 118-d feeding trial with heifers crowded to 133% of capacity at the feed bunk. Inclusion of low-energy forages was effective in reducing both diet energy density and DMI. Concentrations of physically effective fiber (pef) particles did not change during the 24-h period following feeding for either the control or EGH diets; however, this response for pef particles masked the competing (and cancelling) responses for individual large and medium particles, which heifers sorted with discrimination and preference, respectively. Sorting against pef particles was detected for WS, and much more severely for the CF diet. Sorting of forage particles by heifers could not be related to heifer performance. Compared with control (1.16 kg/d), average daily gains (ADG) were reduced by dilution in all cases, but were virtually identical between EGH (0.98 kg/d) and CF (0.97 kg/d), which exhibited no sorting and extensive sorting of pef, respectively. Furthermore, ADG for WS was approximately 0.2 kg/d less than EGH or CF, despite exhibiting sorting characteristics intermediate between EGH and CF. Diets diluted with low-energy forages were formulated to be isonitrogenous and isocaloric; within that context, WS was most effective in reducing DMI and maintaining ADG within typical recommendations for Holstein heifers.

Cardiothoracic ratio may be misleading in the assessment of right- and left-ventricular size in patients with repaired tetralogy of Fallot.

AIM: To assess the relationship between cardiothoracic ratio (CTR) and ventricular and atrial volumes in patients with repaired tetralogy of Fallot (TOF). MATERIALS AND METHODS: Patients with repaired TOF undergoing cardiac magnetic resonance (CMR) and chest radiography within 1 day were included (n = 82; median age: 24.7 years, interquartile range: 21.5-35.9). The CTR was obtained from upright posteroanterior chest roentgenograms. Analyses of CMR images and radiographs were performed in a blinded fashion. RESULTS: There were 35.1% (13/37) of patients with normal CTR (<0.5) who had severe right ventricular (RV) dilatation. There were six patients (13.3%, 6/45) with high CTR with both normal RV and left-ventricular (LV) volumes. CTR did not correlate with either RV or LV volumes but showed a weak correlation with right- and left-atrial volumes (r = 0.43, p = 0.0001; r = 0.27, p = 0.01, respectively). CTR ≥0.5 showed poor ability in the identification of severe RV dilatation (sensitivity: 61.8%, specificity: 50%). The combination of CTR and signs of RV enlargement on lateral radiographs did not improve the diagnostic accuracy of any of those parameters alone. CONCLUSION: CTR in patients with repaired TOF reflected atrial rather than ventricular dilatation. The use of CTR or lateral radiographs in patients with repaired TOF may lead to false conclusions concerning ventricular size.

Relationships between dry matter content, ensiling, ammonia-nitrogen, and ruminal in vitro starch digestibility in high-moisture corn samples.

The objectives of the study were (1) to determine relationships between high-moisture corn (HMC) dry matter (DM), ammonia-N [% of crude protein (CP)], and soluble CP concentrations, and pH, with 7-h ruminal in vitro starch digestibility (ivStarchD), and (2) to evaluate the effect of ensiling on pH, ammonia-N, soluble CP, and ivStarchD measurements in HMC. A data set comprising 6,131 HMC samples (55 to 80% DM) obtained from a commercial feed analysis laboratory was used for this study. Month of sample submittal was assumed to be associated with length of the ensiling period. Data for month of sample submittal were analyzed using Proc Mixed in SAS (SAS Institute Inc., Cary, NC) with month as a fixed effect. Regressions to determine linear and quadratic relationships between ivStarchD and ammonia-N, soluble CP, pH, and DM content were performed using Proc Mixed. The ivStarchD increased by 9 percentage units from October to August of the following year. Similar results were observed for ammonia-N and soluble CP with increases from 1.8 to 4.6% of CP and 31.3 to 46.4% of CP, respectively, from October to August of the following year. Ammonia-N was positively related to ivStarchD (R(2)=0.61). The DM content of HMC at silo removal was negatively related (R(2)=0.47) to ivStarchD with a decrease of 1.6 percentage units in ivStarchD per 1-percentage-unit increase in DM content. The pH of HMC was negatively related to ammonia-N (R(2)=0.53), soluble CP (R(2)=0.57), and ivStarchD (R(2)=0.51). Combined, ammonia-N, DM, soluble CP, and pH provided a good prediction of ivStarchD (adjusted R(2)=0.70). Increasing pH, ammonia-N, soluble CP, and ivStarchD values indicate that HMC may need up to 10 mo of ensiling to reach maximum starch digestibility. Ammonia-N, DM content, soluble CP concentration, and pH are good indicators of ruminal in vitro starch digestibility for high-moisture corn.

Fecal starch as an indicator of total-tract starch digestibility by lactating dairy cows.

To test whether total-tract starch digestibility by lactating dairy cattle could be predicted accurately from concentration of starch in fecal dry matter (FS), data from 8 digestibility trials, 30 diets, and 564 individual starch measurements were compiled from trials conducted at the University of Wisconsin-Madison. Total-tract starch digestibility by individual cows was determined from the concentrations of starch in total mixed rations and feces (rectal grab samples) and concentrations of several external or intrinsic feed markers. Fecal starch concentration was closely and linearly related to total-tract starch digestibility (R(2) = 0.94). Differences in fecal sampling time for FS, such as day within week or week, did not influence determination of FS concentration. In contrast, time of day when fecal samples were taken influenced FS concentration, but the differences were minimal (mean ± standard error of the mean = 0.4 ± 0.1). These data suggest that on-farm collection of feces from individual cows or pens of cows may be sampled only once per day. Fecal pH was not related to FS when FS was only 1 to 3%, indicating that fecal pH was not a good index of FS and not practical as a tool to assess total-tract starch digestibility for lactating dairy cows when fecal starch excretion is low. An equation was developed to predict FS using near-infrared reflectance spectroscopy of dried ground fecal samples; the equation had moderate to good accuracy (R(2) = 0.83-0.94) and a low standard error of prediction. Fecal starch concentration can be used to monitor total-tract starch digestibility.

Fall-grown oat to extend the fall grazing season for replacement dairy heifers.

Our objective was to assess the pasture productivity and forage characteristics of 2 fall-grown oat (Avena sativa L.) cultivars, specifically for extending the grazing season and reducing reliance on harvested forages by replacement dairy heifers. A total of 160 gravid Holstein heifers (80 heifers/yr) were stratified by weight, and assigned to 1 of 10 identical research pens (8 heifers/pen). Initial body weights were 480 ± 43.5 kg in 2011 and 509 ± 39.4 kg in 2012. During both years of the trial, four 1.0-ha pasture replicates were seeded in August with Ogle oat (Schumitsch Seed Inc., Antigo, WI), and 4 separate, but similarly configured, pasture replicates were seeded with Forage Plus oat (Kratz Farms, Slinger, WI). Heifer groups were maintained as units, assigned to specific pastures, and then allowed to graze fall-oat pastures for 6h daily before returning to the barn, where they were offered a forage-based basal total mixed ration. Two heifer groups were retained in confinement (without grazing) as controls and offered the identical total mixed ration as pasture groups. During 2011, available forage mass increased with strong linear and quadratic effects for both cultivars, peaking at almost 9 Mg/ha on October 31. In contrast, forage mass was not affected by evaluation date in 2012, remaining ≤ 2,639 kg/ha across all dates because of droughty climatic conditions. During 2012, Ogle exhibited greater forage mass than Forage Plus across all sampling dates (2,678 vs. 1,856 kg/ha), largely because of its more rapid maturation rate and greater canopy height. Estimates of energy density for oat forage ranged from 59.6 to 69.1% during 2011, and ranged narrowly from 68.4 to 70.4% during 2012. For 2011, responses for both cultivars had strong quadratic character, in which the most energy-dense forages occurred in mid November, largely due to accumulation of water-soluble carbohydrates that reached maximum concentrations of 18.2 and 15.1% for Forage Plus and Ogle, respectively. Across the 2-yr trial, average daily gain for grazing heifer groups tended to be greater than heifers remaining in confinement (0.85 vs. 0.74 kg/d), but both management strategies produced weight gains within reasonable proximity to normal targets for heifers in this weight range. Fall-grown oat should be managed as stockpiled forage for deferred grazing, and good utilization of fall-oat forage can be accomplished by a one-time removal of standing forage, facilitated by a single lead wire advanced daily to prevent waste.

Lipoxygenase activity and sanguinarine production in cell suspension cultures of California poppy (Eschscholtzia californica CHAM.).

In this study we investigated the influence of biotic elicitor (phytopathogenic fungus Botrytis cinerea) and abiotic elicitors (methyljasmonate [MJ] and salicylic acid [SA]) on lipoxygenase (LOX) activity and sanguinarine production in cell suspension cultures of California poppy (Eschscholtzia californica CHAM.). We have observed different time effects of elicitors (10, 24, 48 and 72 h) on LOX activity and production of sanguinarine in in vitro cultures. All elicitors used in the experiments evidently increased the LOX activity and sanguinarine production in contrast to control samples. The highest LOX activities were determined in samples elicitated by MJ after 48 h and 72 h and the lowest LOX activities (in contrast to control samples) were detected after biotic elicitation by Botrytis cinerea. These activities showed about 50% lower level against the activities after MJ elicitation. The maximal amount of sanguinarine was observed after 48 h in MJ treated cultures (429.91 mg/g DCW) in comparision with control samples. Although all elicitors affect the sanguinarine production, effect of SA and biotic elicitor on sanguinarine accumulation in in vitrocultures was not so significant than after MJ elicitation.

Assessment of mould remediation in a healthcare setting following extensive flooding.

BACKGROUND: A new hospital building was close to completion when a large pipe carrying clean water broke, causing extensive flooding. AIM: To determine the flood-associated fungal risk to susceptible patients who would use that building. METHODS: Though standard flood remediation by the builders was relatively straightforward, there was no model for specialist assessment of patient risk due to the flood-associated mould growth. As levels of background airborne fungal spores can be expected to vary significantly over time, we could not use absolute levels to indicate either an excess of airborne fungal spores or successful remediation. Therefore it was decided to use weekly settle plates, exposed at the same time in flooded (test) and equivalent non-flooded (control) areas to compensate for variations in background levels. Flood-related risk was estimated by the ratio between fungal colonies on the test and control sets of settle plates, rather than absolute number. FINDINGS: Whereas the physical flood remediation, including the use of 'anti-fungal' treatments, was completed in three weeks post flooding, fungal contamination in flooded areas took 38 weeks to return to control levels and remained so for a further six weeks of observation. CONCLUSION: By the use of this method, we were able to assure the absence of flood-associated fungal risk to susceptible patients who would use that building. We recommend that infection prevention and control teams consider using this approach should they be faced with similar situations.

Association between genetic variation in a region on chromosome 11 and schizophrenia in large samples from Europe.

Recent molecular studies have implicated common alleles of small to moderate effect and rare alleles with larger effect sizes in the genetic architecture of schizophrenia (SCZ). It is expected that the reliable detection of risk variants with very small effect sizes can only be achieved through the recruitment of very large samples of patients and controls (that is tens of thousands), or large, potentially more homogeneous samples that have been recruited from confined geographical areas using identical diagnostic criteria. Applying the latter strategy, we performed a genome-wide association study (GWAS) of 1169 clinically well characterized and ethnically homogeneous SCZ patients from a confined area of Western Europe (464 from Germany, 705 from The Netherlands) and 3714 ethnically matched controls (1272 and 2442, respectively). In a subsequent follow-up study of our top GWAS results, we included an additional 2569 SCZ patients and 4088 controls (from Germany, The Netherlands and Denmark). Genetic variation in a region on chromosome 11 that contains the candidate genes AMBRA1, DGKZ, CHRM4 and MDK was significantly associated with SCZ in the combined sample (n=11 540; P=3.89 × 10(-9), odds ratio (OR)=1.25). This finding was replicated in 23 206 independent samples of European ancestry (P=0.0029, OR=1.11). In a subsequent imaging genetics study, healthy carriers of the risk allele exhibited altered activation in the cingulate cortex during a cognitive control task. The area of interest is a critical interface between emotion regulation and cognition that is structurally and functionally abnormal in SCZ and bipolar disorder.

Stable right ventricular size and function during short-term follow-up in patients with pulmonary regurgitation after tetralogy of Fallot repair.

AIM: To assess changes in ventricular size and function over time in conservatively treated adult patients with repaired tetralogy of Fallot (TOF) and significant pulmonary regurgitation (PR). MATERIALS AND METHODS: Patients with repaired TOF who had undergone more than one cardiac magnetic resonance study were identified. To confine the cause of ventricular size and function deviation to PR, patients with residual ventricular septal defect, more than mild regurgitation at a valve other than the pulmonary valve, and known coronary artery disease were excluded. RESULTS: The final analysis included 27 adults with PR fraction >20%. During a follow-up of mean 2.1 ± 0.8 years, there was no change in right ventricular (RV) end-diastolic volume (EDV; 162.1 ± 27.6 versus 164 ± 29.6 ml/m(2), p = 0.5). Left ventricular (LV) EDV showed a small decrease (85.1 ± 16.2 versus 81.5 ± 14.1 ml/m(2), p = 0.02). The mean PR fraction, PR volume, and peak RV outflow tract gradient did not change. Additionally, both RV ejection fraction (EF) and LVEF remained stable over the follow-up period (48.1 ± 6.5 versus 48.4 ± 6.7%, p = 0.83, and 57.3 ± 5.4 versus 57.2 ± 5.1 %, p = 0.91, respectively). Only two asymptomatic patients (7.4% of the study group) developed symptoms and the remaining did not deteriorate. CONCLUSION: The RVEDV, RVEF, and LVEF remained stable over a mean follow-up of approximately 2 years in the majority of adult patients after TOF repair with significant PR and a wide range of RVEDV.

Technical note: a modified method to quantify prolamin proteins in dry and high-moisture corn.

Numerous studies have evaluated laboratory methods to quantify prolamin proteins in dry corn; however, the utility of methods to quantify functional prolamins, which impede starch digestibility, in high-moisture corn (HMC) is less defined. As a result, a common rapid turbidimetric (rTM) laboratory procedure was modified (rapid Bradford method, rBM), extracting buffer-soluble proteins before prolamin solubilization in an effort to better quantify functional prolamins in HMC. Twenty samples of dry and HMC were evaluated by rTM and rBM procedures. Prolamin concentration in dry corn, as estimated by rTM or rBM methods, ranged from 6.12 to 2.20g/100 g of DM or 5.46 to 2.41g/100 g of DM, respectively. Dry corn mean prolamin concentrations, as estimated by rTM or rBM methods, were similar at 3.65 or 3.66g/100 g of DM. Prolamin concentration in HMC, as estimated by rTM, ranged from 4.99 to 3.24g/100 g of DM, with a mean prolamin concentration of 4.19g/100 g of DM, but estimation of prolamins in HMC by the rBM method resulted in lower mean (4.19 vs. 3.24g/100 g of DM) prolamin concentration. Prolamin concentration in dry and HMC measured by rTM was negatively related to peak absolute rates (PAR; mL/0.1 g of DM) of in vitro gas production. However, relationships between rTM prolamin concentration and PAR were not homogeneous and were different between dry and HMC. Prolamin proteins as determined by rBM were likewise negatively related to PAR, but corn type did not influence rBM prolamin concentration by PAR relationships. Data suggest that the rBM method defined more similar functional prolamin proteins, which impede starch degradability, in dry and HMC.

Assessment of heifer grazing experience on short-term adaptation to pasture and performance as lactating cows.

A 3-yr study evaluated the carryover effects of dairy heifer grazing experience on behavior and first-lactation performance as dairy cows. Forty-one Holstein and 23 Holstein-Jersey crossbred calves born between January and April 2008 were randomly assigned to 1 of 4 treatments (n=8 per group, 2 groups per treatment) in a completely randomized design. Treatments were combinations of managing dairy heifers in confinement (CNF) or on pasture (PST): grazed yr 1 and 2 (PSTPST); grazed yr 1, but confined yr 2 (PSTCNF); confined yr 1 and grazed yr 2 (CNFPST); or confined yr 1 and 2 (CNFCNF). After calving, all heifers on all treatments were grazed as cows in yr 3. In yr 1, PSTPST and PSTCNF heifers were grazed for 41 d on Italian ryegrass pastures, whereas CNFPST and CNFCNF were housed in bedded-pack pens and fed a TMR. In yr 2, PSTPST and CNFPST heifers grazed Italian ryegrass pasture for 65 d, whereas PSTCNF and CNFCNF remained in confinement. In yr 2, a mid-trial assessment of heifer grazing behavior was made on PSTPST versus CNFPST heifers. Grazing activities were assessed by visual observation and heifer movement measured by portable global positioning system units. Heifers from all treatment groups subsequently calved between January and April in yr 3. All primiparous cows were then allocated to pastures by treatment group, grazed for 61 d, (May through July) in yr 3, with grazing behavior and milk production evaluated while grazing. In yr 2, heifers on the PSTPST treatment spent more time grazing than heifers on the CNFPST treatment (78 vs. 35% of the time) when first exposed to pasture (d 1). On d 1 to 3, PSTPST heifers walked a greater distance than CNFPST heifers; however, PSTPST and CNFPST heifers had similar daily grazing times and walking patterns after 3 d of pasture exposure in yr 2. As lactating cows (yr 3), cows with no (CNFCNF) grazing experience grazed less on d 1 compared with cows with (PSTPST, PSTCNF, or CNFPST) grazing experience. Day-1 grazing times in yr 3 were 62, 59, 76, and 13% of the times for cows with PSTPST, PSTCNF, CNFPST, and CNFCNF grazing experience, respectively. In yr 3, on d 1 to 3, cows with previous grazing experience as heifers (PSTPST, CNFPST, and PSTCNF) walked a greater distance than cows without previous grazing experience (CNFCNF). Milk production was lowest on d 1 to 3 for cows with no previous grazing experience (CNFCNF), but average daily milk production was not different overall over the 61 d of study in yr 3. Results indicate that grazing experiences as a heifer can affect behavior and milk production during a cow's first days on pasture. After a short acclimation period, dairy cows without grazing experience as heifers developed similar grazing behaviors and performance as cows with grazing experience as heifers.

Unique interrelationships between fiber composition, water-soluble carbohydrates, and in vitro gas production for fall-grown oat forages.

Sixty samples of 'ForagePlus' oat were selected from a previous plot study for analysis of in vitro gas production (IVGP) on the basis of 2 factors: (1) high (n=29) or low (n=31) neutral detergent fiber (NDF; 62.7±2.61 and 45.1±3.91%, respectively); and (2) the range of water-soluble carbohydrates (WSC) within the high- and low-NDF groups. For the WSC selection factor, concentrations ranged from 4.7 to 13.4% (mean=7.9±2.06%) and from 3.5 to 19.4% (mean=9.7±4.57%) within high- and low-NDF forages, respectively. Our objectives were to assess the relationships between IVGP and various agronomic or nutritional characteristics for high- and low-NDF fall-oat forages. Cumulative IVGP was fitted to a single-pool nonlinear regression model: Y=MAX × (1 - e ([-)(K)(× (t - lag)])), where Y=cumulative gas produced (mL), MAX=maximum cumulative gas produced with infinite incubation time (mL), K=rate constant, t=incubation time (h), and lag=discrete lag time (h). Generally, cumulative IVGP after 12, 24, 36, or 48h within high-NDF fall-oat forages was negatively correlated with NDF, hemicellulose, lignin, and ash, but positively correlated with WSC, nonfiber carbohydrate (NFC), and total digestible nutrients (TDN). For low-NDF fall-grown oat forages, IVGP was positively correlated with growth stage, canopy height, WSC, NFC, and TDN; negative correlations were observed with ash and crude protein (CP) but not generally with fiber components. These responses were also reflected in multiple regression analysis for high- and low-NDF forages. After 12, 24, or 36h of incubation, cumulative IVGP within high-NDF fall-oat forages was explained by complex regression equations utilizing (lignin:NDF)(2), lignin:NDF, hemicellulose, lignin, and TDN(2) as independent variables (R(2)≥0.43). Within low-NDF fall-grown oat forages, cumulative IVGP at these incubation intervals was explained by positive linear relationships with NFC that also exhibited high coefficients of determination (R(2)≥0.75). Gas production was accelerated at early incubation times within low-NDF forages, specifically in response to large pools of WSC that were most likely to be present as forages approached boot stage by late-fall.

Potential gains in lifetime net merit from genomic testing of cows, heifers, and calves on commercial dairy farms.

The objective of this study was to quantify the gains in genetic potential of replacement females that could be achieved by using genomic testing to facilitate selection and culling decisions on commercial dairy farms. Data were simulated for 100 commercial dairy herds, each with 1,850 cows, heifers, and calves. Parameters of the simulation were based on the US Holstein population, and assumed reliabilities of traditional and genomic predictions matched reliabilities of animals that have been genotyped to date. Selection of the top 10, 20, 30, …, 90% of animals within each age group was based on parent averages and predicted transmitting abilities with or without genomic testing of all animals or subsets of animals that had been presorted by traditional predictions. Average gains in lifetime net merit breeding value of selected females due to genomic testing, minus prorated costs of genotyping the animals and their unselected contemporaries, ranged from $28 (top 90% selected) to $259 (top 20% selected) for heifer calves with no pedigrees, $14 (top 90% selected) to $121 (top 10% selected) for heifer calves with known sires, and $7 (top 90% selected) to $87 (top 20% selected) for heifer calves with full pedigrees. In most cases, gains in genetic merit of selected heifer calves far exceeded prorated genotyping costs, and gains were greater for animals with missing or incomplete pedigree information. Gains in genetic merit due to genomic testing were smaller for lactating cows that had phenotypic records, and in many cases, these gains barely exceeded or failed to exceed genotyping costs. Strategies based on selective genotyping of the top, middle, or bottom 50% of animals after presorting by traditional parent averages or predicted transmitting abilities were cost effective, particularly when pedigrees or phenotypes were available and a relatively small proportion of animals were to be selected or culled. Based on these results, it appears that routine genotyping of heifer calves or yearling heifers can be a cost-effective strategy for enhancing the genetic level of replacement females on commercial dairy farms. Increasing the accuracy of predicted breeding values for young females with genomic testing might lead to synergies with other management tools and strategies, such as propagating genetically superior females using advanced reproductive technologies or selling excess females that were generated by the use of sex-enhanced semen.

A query for effective mean particle size in dry and high-moisture corns.

The objective of this study was to investigate whether prediction of fermentation potential (FP) of dry and high-moisture (HM) corns could be improved by using a concept of effective (e) mean particle size (MPS). A set of FP standards was created by processing a single lot of Reid Yellow Dent (RYD) corn to achieve MPS of 3,778, 2,786, 2,282, 1,808, 1,410, 806, 586, 378, 308, 226, and 105 µm. In vitro gas production of RYD standards was measured, and peak absolute rate (PAR) of gas production (mL/0.2 g of DM/h) was used to establish a standard relationship between PAR and MPS. To identify factors other than MPS affecting FP, the MPS and nutrient composition of 36 diverse samples of dry (n=18) and HM (n=18) corns were determined. Composition included dry matter (DM), crude protein, soluble crude protein, neutral detergent fiber, starch, NH3-N, prolamin protein, and fat. In vitro gas production of undried, unground dry and HM corns was measured, and PAR, time of peak absolute rate (h), maximum cumulative gas production (mL/0.2 g of DM), gas production fractional rate (h⁻¹), and lag (h) were determined. Nonlinear relationships between MPS, defined as the dependent variable, and PAR, as an independent variable, were used to identify FP deviations unexplained by MPS. When no variation in nutritional composition was present (RYD standards), the relationship between PAR and MPS was described by an exponential decay model [RYD_MPS=9,006 × e((-0.452 × PAR)); R²=0.96]. For diverse dry and HM corn populations, the variation in MPS explained by PAR was diminished (R²=0.50). To investigate factors that diminish the relationship between MPS and PAR in diverse corns, relative residual (rr) MPS was determined [rrMPS = (MPS - RYD_MPS)/MPS], where RYD_MPS was predicted from the PAR of diverse dry and HM corn. The rrMPS was most highly related to prolamin protein [rrMPS(dry)=0.58-0.15 × (prolamin protein, % of DM); R²=0.43] and NH3-N [rrMPS(HM)=0.21+0.08 × (NH3-N, % of total N); R²=0.46] for dry and HM corns, respectively. An eMPS was calculated as eMPS = MPS - MPS × rrMPS, where rrMPS was predicted from prolamin protein or NH3-N concentration in dry and HM corn, respectively. The natural logarithm of eMPS accounted for 84% of the variability in PAR and 53% of the variability in the fractional rate of gas production. Calculating eMPS by adjusting the MPS of dry corn for prolamin and HM corn for NH3-N concentration improved the assessment of industry corn FP.