Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 47
Filtrar
Más filtros

Banco de datos
País/Región como asunto
Tipo del documento
Intervalo de año de publicación
1.
FASEB J ; 34(6): 7283-7294, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-32277848

RESUMEN

MicroRNAs (miRNAs) have been reported to directly alter the virus life cycle and virus-host interactions, and so are considered promising molecules for controlling virus infection. In the present study, we observed that miR-155 time-dependently downregulated upon dengue virus (DENV) infection. In contrast, exogenous overexpression of miR-155 appeared to limit viral replication in vitro, suggesting that the low levels of miR-155 would be beneficial for DENV replication. In vivo, overexpression of miR-155 protected ICR suckling mice from the life-threatening effects of DENV infection and reduced virus propagation. Further investigation revealed that the anti-DENV activity of miR-155 was due to target Bach1, resulting in the induction of the heme oxygenase-1 (HO-1)-mediated inhibition of DENV NS2B/NS3 protease activity, ultimately leading to induction of antiviral interferon responses, including interferon-induced protein kinase R (PKR), 2'-5'-oligoadenylate synthetase 1 (OAS1), OAS2, and OAS3 expression, against DENV replication. Collectively, our results provide a promising new strategy to manage DENV infection by modulation of miR-155 expression.


Asunto(s)
Antivirales/farmacología , Virus del Dengue/efectos de los fármacos , Dengue/tratamiento farmacológico , Dengue/genética , Hemo-Oxigenasa 1/genética , Interferones/farmacología , Proteínas de la Membrana/genética , MicroARNs/genética , Animales , Línea Celular , Línea Celular Tumoral , Cricetinae , Dengue/virología , Humanos , Ratones , Ratones Endogámicos ICR , Replicación Viral/efectos de los fármacos
2.
Biochim Biophys Acta Mol Cell Biol Lipids ; 1863(8): 844-856, 2018 08.
Artículo en Inglés | MEDLINE | ID: mdl-29678641

RESUMEN

Nonalcoholic fatty liver disease (NAFLD) has been associated with the function and changes in expression levels of microRNAs (miRs). MiR-7 has been proven to play an important role in many cellular processes; however, its functions in the context of liver lipogenesis remain unknown. We applied the microRNA-sponge (miR-SP) technology and generated transgenic miR-7a-SP models (hC7aSP and bC7aSP), which disrupted the activities of hepatic miR-7a and induced the early onset of NAFLD and nonalcoholic steatohepatitis (NASH) in zebrafish. We identified a novel miR-7a target, YY1, and demonstrated novel miR-7a functions to regulate zebrafish hepatic lipid metabolism by controlling YY1 stabilization through the regulation of the expression of lipogenic signaling pathways. Correspondingly, liver specific miR-7a depletion functionally promoted lipid accumulation in hC7ASP livers. NASH hC7aSP increased the expression of inflammatory genes (il-1b, il-6, tnf-α, ifn-γ, nfkb2, and NF-kB) and endoplasmic reticulum stress markers (atf6, ern2, ire1, perk, hspa5 and ddit3). Molecular analysis revealed that miR-7a-SP can stabilize YY1 expression and contribute to the accumulation of hepatic triglycerides by reducing the CHOP-10 expression in the hC7aSP and then inducing the transactivation of C/EBP-α and PPAR-γ expression. PPAR-γ antagonists and miR-7a mimic treatment ameliorate hC7aSP NASH phenotypes. CONCLUSION: Our results suggest that miR-7a-SP acts as a lipid enhancer by directly increasing YY1 stability to disrupt CHOP-10-dependent suppression of lipogenic pathways, resulting in increased lipid accumulation. MiR-7a expression improves liver steatosis and steatohepatitis in hC7aSPs, which suggests a novel strategy for the prevention and early treatment of NASH in humans.


Asunto(s)
Vías Biosintéticas/genética , Modelos Animales de Enfermedad , Lipogénesis/genética , MicroARNs/metabolismo , Enfermedad del Hígado Graso no Alcohólico/genética , Factor de Transcripción YY1/genética , Proteínas de Pez Cebra/genética , Pez Cebra , Animales , Animales Modificados Genéticamente , Línea Celular , Chaperón BiP del Retículo Endoplásmico , Femenino , Humanos , Hígado/metabolismo , Hígado/patología , Masculino , MicroARNs/antagonistas & inhibidores , MicroARNs/genética , Enfermedad del Hígado Graso no Alcohólico/patología , Oncorhynchus mykiss , PPAR gamma/antagonistas & inhibidores , PPAR gamma/metabolismo , Estabilidad Proteica , Factor de Transcripción CHOP/metabolismo , Factor de Transcripción YY1/metabolismo , Proteínas de Pez Cebra/metabolismo
3.
Electrophoresis ; 39(19): 2460-2470, 2018 10.
Artículo en Inglés | MEDLINE | ID: mdl-30004128

RESUMEN

This study uses negative dielectrophoresis and AC electroosmosis as a driving mechanism and presents an electrically driven microconcentrator that concentrates the sample in the region exterior to the electrodes (termed as exterior-electrode electrically driven microconcentrator in this paper). The proposed microconcentrator uses a 3-D face-to-face electrode pair; the top electrode is a relatively large planar electrode, and the bottom electrode is formed with three to six long and thin electrodes connected into an open ring. The sample is brought to the vicinity of the open electrode at the bottom by electroosmotic flow; then, negative dielectrophoresis is used to push the sample away from the electrode and concentrate it in the region surrounded by the open ring electrode. Concentration using an exterior-electrode electrically driven microconcentrator offers promise for convenient use in conjunction with relevant detection systems. The results indicate that for the proposed exterior-electrode electrically driven microconcentrator, the optimal frequency is 100 kHz and the optimal voltage is 13 Vp-p . The corner concentration process at the corners of the bottom open electrodes enables the multi-corner electrodes to exhibit better concentration results than that exhibited by semicircular-shaped electrodes. The concentration performance is most favorable when the shape of the open electrode at the bottom is a five-vertex electrode, enabling a concentration enhancement factor of 55 times for a latex particle solution and 11 times for E. coli. The experimental results also demonstrate that the concentration phenomenon in this study is not induced by non-specific adsorption and can be repeated multiple times.


Asunto(s)
Electroósmosis/instrumentación , Electroforesis/instrumentación , Electrodos , Diseño de Equipo , Escherichia coli/aislamiento & purificación , Microesferas , Modelos Químicos
7.
Int J Med Sci ; 14(9): 885-890, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28824326

RESUMEN

Lewis antigens related to the ABO blood group are fucosylated oligosaccharides and are synthesized by specific glycosyltransferases (FUTs). FUTs are involved in various biological processes including cell adhesion and tumor progression. The fucosyltransferase-2 gene (FUT2) encodes alpha (1,2) fucosyltransferase, which is responsible for the addition of the alpha (1,2)-linkage of fucose to glycans. Aberrant fucosylation occurs frequently during the development and progression of hepatocellular carcinoma (HCC). However, the association of FUT2 polymorphisms with HCC development has not been studied. Therefore, we aimed to investigate the association of FUT2 polymorphisms with demographic, etiological, and clinical characteristics and with susceptibility to HCC. In this study, a total of 339 patients and 720 controls were recruited. The genotypes of FUT2 at four single-nucleotide polymorphisms (SNPs; rs281377, rs1047781, rs601338, and rs602662) were detected by real-time polymerase chain reaction from these samples. Compared with the wild-type genotype at SNP rs1047781, which is homozygous for nucleotides AA, at least one polymorphic T allele (AT or TT) displayed significant association with clinical stage (p = 0.048) and tumor size (p = 0.022). Our study strongly implicates the polymorphic locus rs1047781 of FUT2 as being associated with HCC development.


Asunto(s)
Carcinoma Hepatocelular/genética , Fucosiltransferasas/genética , Predisposición Genética a la Enfermedad , Neoplasias Hepáticas/genética , Adulto , Alelos , Carcinoma Hepatocelular/patología , Femenino , Estudios de Asociación Genética , Genotipo , Humanos , Neoplasias Hepáticas/patología , Masculino , Persona de Mediana Edad , Factores de Riesgo , Galactósido 2-alfa-L-Fucosiltransferasa
9.
Int J Mol Sci ; 19(1)2017 Dec 29.
Artículo en Inglés | MEDLINE | ID: mdl-29286302

RESUMEN

miR-27b has emerged as a regulatory hub in cholesterol and lipid metabolism, and as a potential therapeutic target for treating atherosclerosis and obesity. However, the impact of miR-27b on lipid levels in vivo remains to be determined. Zebrafish lipids are normally stored as triacylglycerols (TGs) and their main storage sites are visceral, intramuscular, and subcutaneous lipid depots, and not blood vessels and liver. In this study, we applied microRNA-sponge (miR-SP) technology and generated zebrafish expressing transgenic miR-27b-SP (C27bSPs), which disrupted endogenous miR-27b activity and induced intravascular lipid accumulation (hyperlipidemia) and the early onset of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Oil Red O staining predominantly increased in the blood vessels and livers of larvae and juvenile C27bSPs, indicating that miR-27b depletion functionally promoted lipid accumulation. C27bSPs also showed an increased weight gain with larger fat pads, resulting from adipocyte hyperplasia. Molecular analysis revealed that miR-27b depletion increased the expression of genes that are associated with lipogenesis and the endoplasmic reticulum (ER). Moreover, miR-27b-SP increased peroxisome proliferator-activated receptor γ (PPAR-γ), CCAAT enhancer binding protein-α (C/EBP-α, and sterol regulatory element binding transcription factor 1c (SREBP-1c) expression and contributed to lipogenesis and adipogenesis. CONCLUSION: Our results suggest that miR-27b-SP acts as a lipid enhancer by directly increasing the expression of several lipogenic/adipogenic transcriptional factors, resulting in increased lipogenesis and adipogenesis. In this study, miR-27b expression improved lipid metabolism in C27bSPs, which suggests that miR-27b is an important lipogenic factor in regulating early onset of hyperlipidemia and adipogenesis in zebrafish.


Asunto(s)
Adipogénesis/genética , Hiperlipidemias/genética , Hiperplasia/genética , Larva/genética , MicroARNs/genética , Enfermedad del Hígado Graso no Alcohólico/genética , Adipocitos/metabolismo , Adipocitos/patología , Animales , Animales Modificados Genéticamente , Antagomirs/genética , Antagomirs/metabolismo , Secuencia de Bases , Proteínas Potenciadoras de Unión a CCAAT/genética , Proteínas Potenciadoras de Unión a CCAAT/metabolismo , Modelos Animales de Enfermedad , Embrión no Mamífero , Regulación del Desarrollo de la Expresión Génica , Genes Reporteros , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Hiperlipidemias/metabolismo , Hiperlipidemias/patología , Hiperplasia/metabolismo , Hiperplasia/patología , Larva/crecimiento & desarrollo , Larva/metabolismo , Metabolismo de los Lípidos/genética , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , MicroARNs/antagonistas & inhibidores , MicroARNs/metabolismo , Enfermedad del Hígado Graso no Alcohólico/metabolismo , Enfermedad del Hígado Graso no Alcohólico/patología , PPAR gamma/genética , PPAR gamma/metabolismo , Transducción de Señal , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/genética , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo , Pez Cebra , Proteína Fluorescente Roja
10.
Neurobiol Learn Mem ; 131: 101-8, 2016 05.
Artículo en Inglés | MEDLINE | ID: mdl-27003118

RESUMEN

The ability to select between actions that are more vs. less likely to be reinforced is necessary for survival and navigation of a changing environment. A task termed "response-outcome contingency degradation" can be used in the laboratory to determine whether rodents behave according to such goal-directed response strategies. In one iteration of this task, rodents are trained to perform two food-reinforced behaviors, then the predictive relationship between one instrumental response and the associated outcome is modified by providing the reinforcer associated with that response non-contingently. During a subsequent probe test, animals can select between the two trained responses. Preferential engagement of the behavior most likely to be reinforced is considered goal-directed, while non-selective responding is considered a failure in response-outcome conditioning, or "habitual." This test has largely been used with rats, and less so with mice. Here we compiled data collected from several cohorts of mice tested in our lab between 2012 and 2015. Mice were bred on either a C57BL/6 or predominantly BALB/c strain background. We report that both strains of mice can use information acquired as a result of instrumental contingency degradation training to select amongst multiple response options the response most likely to be reinforced. Mice differ, however, during the training sessions when the familiar response-outcome contingency is being violated. BALB/c mice readily generate perseverative or habit-like response strategies when the only available response is unlikely to be reinforced, while C57BL/6 mice more readily inhibit responding. These findings provide evidence of strain differences in response strategies when an anticipated reinforcer is unlikely to be delivered.


Asunto(s)
Conducta Animal/fisiología , Conducta de Elección/fisiología , Ratones Endogámicos/fisiología , Corteza Prefrontal/fisiología , Refuerzo en Psicología , Animales , Inhibición Psicológica , Ratones , Ratones Endogámicos BALB C/fisiología , Ratones Endogámicos C57BL/fisiología , Recompensa
11.
J Org Chem ; 80(13): 6669-78, 2015 Jul 02.
Artículo en Inglés | MEDLINE | ID: mdl-26029977

RESUMEN

2-(Acylmethylene)pyrrolidine derivatives were synthesized via intermolecular decarbonylative Mannich reaction from various methyl ketones and 1-alkyl-1-pyrroliniums, generated in situ from 1-alkylprolines. This approach mimics the biosynthetic pathway and provides a direct access to a series of 2-(acylmethylene)pyrrolidine alkaloids, including hygrine, N-methylruspolinone, dehydrodarlinine, and ruspolinone.


Asunto(s)
Alcaloides/síntesis química , Pirrolidinas/síntesis química , Alcaloides/química , Biomimética , Estructura Molecular , Pirrolidinas/química
12.
Molecules ; 20(4): 6844-55, 2015 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-25913925

RESUMEN

One new benzofuran, (2R)-(2',4'-dihydroxybenzyl)-6,7-methylenedioxy-2,3-dihydrobenzofuran (1), one new phenylisocoumarin, 3-(2'-hydroxyphenyl)-6,8-dihydroxy-7-methoxy-isocoumarin (2), and one new benzofuroisocoumarin, platyphyllarin C (3), were isolated from the ethanolic extract of Liriope platyphylla aerial parts, along with seventeen known compounds. The structures of the isolates were established by spectroscopic analysis and comparison with the literature data. The results indicated that structures 1-3 are uncommon in Nature. Benzofuroisocoumarin 4, flavonoids 9, 10, and 13-15, and homoisoflavonoids 19 and 20 exhibited significant binding activity to estrogen-receptor α and/or ß as demonstrated by the SEAP reporter assay system in an MCF-7 cell-line.


Asunto(s)
Estrógenos/farmacología , Liriope (Planta)/química , Fitoquímicos/química , Componentes Aéreos de las Plantas/química , Extractos Vegetales/química , Cromanos/química , Cromanos/aislamiento & purificación , Cromanos/farmacología , Receptor alfa de Estrógeno/metabolismo , Receptor beta de Estrógeno/metabolismo , Estrógenos/química , Estrógenos/aislamiento & purificación , Humanos , Isocumarinas/química , Isocumarinas/aislamiento & purificación , Isocumarinas/farmacología , Células MCF-7 , Estructura Molecular , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacología
13.
BMC Neurol ; 14: 100, 2014 May 07.
Artículo en Inglés | MEDLINE | ID: mdl-24885357

RESUMEN

BACKGROUND: The 2010 Revisions to the McDonald Criteria have established that dissemination in time (DIT) of multiple sclerosis (MS) can be demonstrated by simultaneous presence of asymptomatic gadolinium-enhancing and nonenhancing lesions on a single magnetic resonance imaging (MRI). However, gadolinium-based contrast agents (GBCAs) have contraindications. Diffusion-weighted imaging (DWI) can detect diffusion alterations in active inflammatory lesions. The purpose of this study was to investigate if DWI can be an alternative to contrast-enhanced T1-weighted imaging (CE T1WI) for demonstrating DIT in MS. METHODS: We selected patients with clinically definite MS and evaluated their baseline brain MRI. Asymptomatic lesions were identified as either hyperintense or nonhyperintense on DWI and enhancing or nonenhancing on CE T1WI. Fisher's exact test was performed to determine whether the hyperintensity on DWI was related to the enhancement on CE T1WI (P < 0.05). The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of the DWI to predict lesion enhancement were calculated. RESULTS: Twenty-two patients with 384 demyelinating lesions that were hyperintense on T2-weighted imaging and more than 3 mm in size were recruited. The diffusion hyperintensity and lesion enhancement were significantly correlated (P <0.001). The sensitivity, specificity, PPV, NPV and accuracy were 100%, 67.9%, 32.3%, 100% and 72.1%, respectively. CONCLUSIONS: A hyperintense DWI finding does not necessarily overlap with contrast enhancement. There are many false positives, possibly representing other stages of lesion development. Although DWI may not replace CE T1WI imaging to demonstrate DIT due to the low PPV, it may serve as a screening MRI sequence where the use of GBCAs is a concern.


Asunto(s)
Imagen de Difusión por Resonancia Magnética/métodos , Imagen por Resonancia Magnética/métodos , Esclerosis Múltiple/diagnóstico , Adolescente , Adulto , Encéfalo/patología , Medios de Contraste/efectos adversos , Enfermedades Desmielinizantes/patología , Progresión de la Enfermedad , Femenino , Gadolinio/efectos adversos , Humanos , Masculino , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Reproducibilidad de los Resultados , Adulto Joven
14.
Cancers (Basel) ; 16(8)2024 Apr 22.
Artículo en Inglés | MEDLINE | ID: mdl-38672694

RESUMEN

The Cancers Editorial Office retracts the article, "MicroRNA-21 Plays Multiple Oncometabolic Roles in the Process of NAFLD-Related Hepatocellular Carcinoma via PI3K/AKT, TGF-ß, and STAT3 Signaling" [...].

15.
Transgenic Res ; 22(4): 823-38, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23315130

RESUMEN

The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish liver, we conditionally expressed CB1R proteins using a liver-specific Tet(off) transgenic system. In this study, we found hepatic lipid accumulation in CB1R transgenic zebrafish (CB) without doxycycline treatment (-Dox) and a suppression of CB1R expression, resulting in the loss of lipid accumulation in the livers of CB fish that received doxycycline treatment (+Dox). Oil Red O (ORO)-stained hepatocytes were predominant in the liver buds of CB-Dox larvae, indicating that CB1R functionally promotes lipid accumulation during CB hepatogenesis. More than 73 % of CB-Dox adults showed increased lipid content, which leads, in turn, to steatosis. Liver histology and ORO staining of CB-Dox hepatocytes also indicated the accumulation of fatty droplets in the CB liver samples, consistent with the specific pathological features of liver steatosis or steatohepatitis. We also found that hepatic CB1R overexpression accompanies the stimulation of the lipogenic transcription factor SREBP-1c and its target enzymes, acetyl coenzyme-A carboxylase-1 (ACC1) and fatty acid synthase (FAS), and increases de novo fatty acid synthesis. This study is the first to report CB1R as a potential hepatic stimulator for zebrafish liver steatosis.


Asunto(s)
Metabolismo de los Lípidos/genética , Hígado/metabolismo , Obesidad/genética , Receptor Cannabinoide CB1/metabolismo , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/genética , Adulto , Animales , Animales Modificados Genéticamente , Hepatocitos/metabolismo , Humanos , Lípidos/toxicidad , Obesidad/metabolismo , Receptor Cannabinoide CB1/genética , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo , Pez Cebra/genética , Pez Cebra/metabolismo
16.
Biochim Biophys Acta ; 1811(9): 536-48, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21722753

RESUMEN

Gankyrin is a small ankyrin-repeat protein that previous research has confirmed to be overexpressed in hepatocellular carcinoma (HCC). Although relevant literature has reported on gankyrin functions in cellular proliferation and tumorigenesis, the exact role of gankyrin is poorly understood in animal model systems. This study analyzed hepatic lipid accumulation in gankyrin transgenic (GK) zebrafish. Bromodeoxyuridine (BrdU)-positive cells were predominantly increased in the liver bud of GK larvae, indicating that gankyrin functionally promoted cell proliferation at the larval stage in GK fish. However, over 90% of the viable GK adults showed an increased lipid content, leading in turn to liver steatosis. Liver histology and oil red O staining also indicated the accumulation of fatty droplets in GK fish, consistent with the specific pathological features of severe steatosis. Molecular analysis revealed that gankyrin overexpression induced hepatic steatosis and modulated the expression profiles of four hepatic microRNAs, miR-16, miR-27b, miR-122, and miR-126, and 22 genes involved in lipid metabolism. Moreover, significantly increased hepatic cell apoptosis resulted in liver damage in GK adults, leading to liver failure and death after approximately 10months. This study is the first to report gankyrin as a potential link between microRNAs and liver steatosis in zebrafish.


Asunto(s)
Hígado Graso/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Proteínas Proto-Oncogénicas/metabolismo , Proteínas de Pez Cebra/metabolismo , Pez Cebra/anatomía & histología , Pez Cebra/fisiología , Animales , Animales Modificados Genéticamente , Secuencia de Bases , Hígado Graso/patología , Femenino , Humanos , Metabolismo de los Lípidos , Hígado/patología , Hígado/fisiología , Masculino , MicroARNs/metabolismo , Datos de Secuencia Molecular , Complejo de la Endopetidasa Proteasomal/genética , Proteínas Proto-Oncogénicas/genética , Pez Cebra/genética , Proteínas de Pez Cebra/genética
17.
Opt Express ; 20(21): 23954-9, 2012 Oct 08.
Artículo en Inglés | MEDLINE | ID: mdl-23188361

RESUMEN

We analyze the non-uniform electric field distribution in an in-plane-switching blue phase liquid crystal (IPS-BPLC) cell and use the director model to simulate the electro-optical properties of an IPS-BPLC cell using a commercial simulator. The calculated results are in good agreement with the experimental data.


Asunto(s)
Cristales Líquidos/química , Cristales Líquidos/efectos de la radiación , Modelos Químicos , Refractometría/instrumentación , Color , Simulación por Computador , Campos Electromagnéticos , Diseño de Equipo , Análisis de Falla de Equipo , Luz , Dispersión de Radiación
18.
Ann Plast Surg ; 69(6): 656-62, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23154338

RESUMEN

INTRODUCTION: The promotion of wound healing using dermal substitutes has become increasingly widespread, but the outcomes of substitute-assisted healing remain functionally deficient. Adipose-derived stem cells (ASCs) have been investigated widely in regenerative medicine and tissue engineering, and they have the potential to enhance wound healing. In this study, we focused on investigating the effects and mechanism of ASCs combined with an acellular dermal matrix (ADM) to treat full-thickness cutaneous wounds in a murine model. METHODS: The ADM was prepared from the dorsal skin of nude mice by decellularization by treatment with trypsin followed by Triton X-100. The human ASCs were isolated and cultured from abdominal lipoaspirate. We created a rounded, 8-mm, full-thickness cutaneous wound in nude mice and divided the mice into the following 4 groups: silicon sheet cover only, silicon sheet with spreading ASCs, ADM only, and ASCs seeded on ADM. The granulation thickness was evaluated by histology after 7 days. Further comparisons between the ADM only and ASC-seeded ADM groups were undertaken by assessing the reepithelialization ratio and blood vessel density at postoperative days 9 and 14. Statistical analyses were conducted using Student 2-tailed t test. Immunofluorescent histology and ASC labeling were also performed to identify possible mechanisms. RESULTS: The ADM was successfully prepared, and the cytometry analysis and differentiation assay provided the characterization of the human ASCs. A marked improvement in granulation thickness was detected in the ADM-ASC group in comparison with other 3 groups. A significantly increased rate of reepithelialization in the ADM-ASC group (80 ± 6%) compared to the ADM only group (60 ± 7%) was noted on postoperative day 9. The blood vessel density was evidently increased in the ADM-ASC group (7.79 ± 0.40 vessels per field) compared to the ADM only group (5.66 ± 0.23 vessels) on day 14. Cell tracking experiments demonstrated that labeled ASCs were colocalized with staining for VEGF or endothelial cell maker vWF after the transplantation of ADM-ASCs on postoperative day 14. CONCLUSIONS: Adipose-derived stem cells seeded on an ADM can enhance wound healing, promote angiogenesis, and contribute to newly formed vasculature, and VEGF-expressing ASCs can be detected after transplantation. This model could be used to improve the other clinical applications of ASCs and to decipher the detailed mechanism by which ASCs interact with wound tissue.


Asunto(s)
Dermis Acelular , Tejido Adiposo/citología , Trasplante de Piel , Piel Artificial , Trasplante de Células Madre , Ingeniería de Tejidos , Cicatrización de Heridas , Adipocitos/citología , Adulto , Animales , Diferenciación Celular , Células Cultivadas , Condrocitos/citología , Femenino , Técnica del Anticuerpo Fluorescente , Humanos , Ratones , Ratones Desnudos , Osteoblastos/citología , Piel/irrigación sanguínea , Piel/lesiones , Piel/patología
19.
J Hazard Mater ; 432: 128726, 2022 06 15.
Artículo en Inglés | MEDLINE | ID: mdl-35316633

RESUMEN

In-situ chemical oxidation (ISCO) and permeable reactive barrier (PRB) have been used in field practices for contaminated groundwater remediation. In this lab-scale study, a novel system integrating ISCO and PRB using peroxydisulfate (PDS) as the oxidant and copper oxide (CuO) as the reactive barrier material was developed for the removal of 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP). The influences of chlorophenol concentration and flow rate on the system performance were first evaluated using synthetic solutions. The removal efficiencies of target chlorophenols were greater than 90% when sufficient PDS was supplied ([PDS]/[chlorophenol]>1). It was also found that the removal efficiencies decreased with the increasing chlorophenol concentrations (10-150 µM) and flow rates (1.8-14.4 mL/min). When three real groundwaters were employed, the removal efficiencies of 2,4-DCP and 2,4,6-TCP slightly reduced to 90% and 85%, respectively. For PCP, the removal efficiency dropped to 20% in two groundwaters with relatively high levels of alkalinity. The influences of pH and TOC were found to be insignificant for the range investigated (pH 6.5-8.7 and TOC = 0.4-1.5 mgC/L). The reduced removal efficiency could be due to the formation of weaker radicals and the stronger competition between bicarbonate ions and PDS for the activation sites on the CuO surfaces.


Asunto(s)
Clorofenoles , Contaminantes Químicos del Agua , Cobre , Oxidación-Reducción , Óxidos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA