RESUMEN
Astragalin, isolated from flowers of Rosa chinensis Jacq., is a kind of flavonoid, with anti-inflammatory, antioxidant, antiviral, analgesic, antibacterial, antiallergic, and antihepatotoxic effects. However, no studieson the procoagulant effect of astragalin have been reported. This study aimed to investigate the procoagulant activity of astragalin and its mechanism. Its procoagulant effect was investigated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) in vitro, and a rat model established by heparin sodium was used to evaluate the mechanism for the procoagulant effect in vivo. The results showed that astragalin had good procoagulant effects compared with the control group in vitro. Compared with the model group in vivo, astragalin could shorten the coagulation time and significantly increase the number of platelets. Meanwhile, astragalin could significantly reduce the effectual time of PT and APTT and increase the content of FIB. The contents of 6-keto-PGF1α and eNOS significantly decreased. Astragalin could increase whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte sedimentation rate (ESR) and packedcell volume (PCV). All of the above revealed that astragalin had good procoagulant effects by promoting the intrinsic and extrinsic coagulation system.
Asunto(s)
Coagulación Sanguínea/efectos de los fármacos , Fibrinógeno/metabolismo , Quempferoles/farmacología , Agregación Plaquetaria/efectos de los fármacos , 6-Cetoprostaglandina F1 alfa/metabolismo , Animales , Pruebas de Coagulación Sanguínea , Sedimentación Sanguínea/efectos de los fármacos , Viscosidad Sanguínea/efectos de los fármacos , Medicamentos Herbarios Chinos/metabolismo , Medicamentos Herbarios Chinos/farmacología , Endotelina-1/metabolismo , Femenino , Flavonoides/metabolismo , Flavonoides/farmacología , Quempferoles/química , Quempferoles/aislamiento & purificación , Quempferoles/metabolismo , Masculino , Óxido Nítrico Sintasa de Tipo III/metabolismo , Tiempo de Tromboplastina Parcial , Tiempo de Protrombina , Conejos , Ratas , Ratas Sprague-Dawley , Rosaceae/química , Tiempo de Trombina , Tromboxano B2/metabolismoRESUMEN
The leaves and bark of Syringa oblata Lindl are used as folk medicine which has heat-clearing, detoxifying, dampness-removing and jaundice-relieving effects. There are many studies about leaves of S. oblata because of its abundant resource, however, less reports about the components of S. oblata flowers. The previous studies on S. oblate flowers were mainly focused on the volatile components and its traditional pharmacological activity. Thus, this study aimed to investigate the nonvolatile chemical constituents and the coagulation activity of S. oblate flowers. The chemical constituents of S. oblate flowers were isolated with various column chromatographies and coagulation activity of the major constituents was investigated by assaying the activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB) on plasma of rabbit in vitro. Fifteen known compounds (namely compound 1-15) were isolated from S. oblata flowers. Compound 6, 10, 11 and 14 were isolated from Syringa genus for the first time. Compound 1, 2, 4, 5, 8 and 9 were isolated from the plant for the first time. The results of coagulation activity showed that water part of S. oblate flowers, lauric acid and kaempferol-rutinose significantly shorten PT (P < 0.001), TT (P < 0.001) and APTT (P < 0.001) compared with blank group, thus revealed that water extract of S. oblate flowers, lauric acid and kaempferol-rutinose possessed the procoagulant activity, but the effects were not better than that of Yunnan Baiyao as positive control.