Evidence for a semisolid phase state of aerosols and droplets relevant to the airborne and surface survival of pathogens.

The phase state of respiratory aerosols and droplets has been linked to the humidity-dependent survival of pathogens such as SARS-CoV-2. To inform strategies to mitigate the spread of infectious disease, it is thus necessary to understand the humidity-dependent phase changes associated with the particles in which pathogens are suspended. Here, we study phase changes of levitated aerosols and droplets composed of model respiratory compounds (salt and protein) and growth media (organic-inorganic mixtures commonly used in studies of pathogen survival) with decreasing relative humidity (RH). Efflorescence was suppressed in many particle compositions and thus unlikely to fully account for the humidity-dependent survival of viruses. Rather, we identify organic-based, semisolid phase states that form under equilibrium conditions at intermediate RH (45 to 80%). A higher-protein content causes particles to exist in a semisolid state under a wider range of RH conditions. Diffusion and, thus, disinfection kinetics are expected to be inhibited in these semisolid states. These observations suggest that organic-based, semisolid states are an important consideration to account for the recovery of virus viability at low RH observed in previous studies. We propose a mechanism in which the semisolid phase shields pathogens from inactivation by hindering the diffusion of solutes. This suggests that the exogenous lifetime of pathogens will depend, in part, on the organic composition of the carrier respiratory particle and thus its origin in the respiratory tract. Furthermore, this work highlights the importance of accounting for spatial heterogeneities and time-dependent changes in the properties of aerosols and droplets undergoing evaporation in studies of pathogen viability.

Seeded Crystal Growth of Internally Mixed Organic-Inorganic Aerosols: Impact of Organic Phase State.

Atmospheric aerosols are complex with both inorganic and organic components. The soluble inorganics can transition between aqueous and crystalline phases through efflorescence and deliquescence. This study focuses on the efflorescence of (NH4)2SO4/organic particles by seeded crystal growth through contact with a crystal of (NH4)2SO4. Seeded crystal growth is known to effectively shut down supersaturation of aqueous aerosols. Here, we investigate whether organics can inhibit seeded crystal growth. We demonstrate that poly(ethylene glycol) 400 (PEG-400), which phase-separates from the aqueous (NH4)2SO4 and forms a core-shell structure, did not inhibit seeded crystal growth of (NH4)2SO4 at all relative humidity (RH) values below deliquescence RH. The PEG-400 layer was not viscous enough to prevent the diffusion of species through the coating. In contrast, we find that although raffinose, which stays homogeneously mixed with (NH4)2SO4, did not inhibit seeded crystal growth at RH > 45%, it did inhibit heterogeneous efflorescence at lower humidities. Viscosity measurements using an electrodynamic balance show a significant increase in viscosity as humidity was lowered, suggesting that inhibited diffusion of water and ions prevented efflorescence. The observed efflorescence at the higher RH also demonstrates that collisions can induce efflorescence of mixed aerosols that would otherwise not homogeneously effloresce.

Carbohydrate vitrification in aerosolized saliva is associated with the humidity-dependent infectious potential of airborne coronavirus.

An accepted murine analogue for the environmental behavior of human SARS coronaviruses was aerosolized in microdroplets of its culture media and saliva to observe the decay of its airborne infectious potential under relative humidity (RH) conditions relevant to conditioned indoor air. Contained in a dark, 10 m3 chamber maintained at 22°C, murine hepatitis virus (MHV) was entrained in artificial saliva particles that were aerosolized in size distributions that mimic SARS-CoV-2 virus expelled from infected humans' respiration. As judged by quantitative PCR, more than 95% of the airborne MHV aerosolized was recovered from microdroplets with mean aerodynamic diameters between 0.56 and 5.6 µm. As judged by its half-life, calculated from the median tissue culture infectious dose (TCID50), saliva was protective of airborne murine coronavirus through a RH range recommended for conditioned indoor air (60% < RH < 40%; average half-life = 60 minutes). However, its average half-life doubled to 120 minutes when RH was maintained at 25%. Saliva microaerosol was dominated by carbohydrates, which presented hallmarks of vitrification without efflorescence at low RH. These results suggest that dehydrating carbohydrates can affect the infectious potential coronaviruses exhibit while airborne, significantly extending their persistence under the drier humidity conditions encountered indoors.