RESUMEN
Nocardioides sp. strain JS614 grows on ethene and vinyl chloride (VC) as sole carbon and energy sources and is of interest for bioremediation and biocatalysis. Sequencing of the complete genome of JS614 provides insight into the genetic basis of alkene oxidation, supports ongoing research into the physiology and biochemistry of growth on ethene and VC, and provides biomarkers to facilitate detection of VC/ethene oxidizers in the environment. This is the first genome sequence from the genus Nocardioides and the first genome of a VC/ethene-oxidizing bacterium.
Asunto(s)
Actinomycetales/genética , ADN Bacteriano/química , ADN Bacteriano/genética , Genoma Bacteriano , Actinomycetales/metabolismo , Etilenos/metabolismo , Datos de Secuencia Molecular , Oxidación-Reducción , Análisis de Secuencia de ADN , Cloruro de Vinilo/metabolismoRESUMEN
Chromosome 19 has the highest gene density of all human chromosomes, more than double the genome-wide average. The large clustered gene families, corresponding high G + C content, CpG islands and density of repetitive DNA indicate a chromosome rich in biological and evolutionary significance. Here we describe 55.8 million base pairs of highly accurate finished sequence representing 99.9% of the euchromatin portion of the chromosome. Manual curation of gene loci reveals 1,461 protein-coding genes and 321 pseudogenes. Among these are genes directly implicated in mendelian disorders, including familial hypercholesterolaemia and insulin-resistant diabetes. Nearly one-quarter of these genes belong to tandemly arranged families, encompassing more than 25% of the chromosome. Comparative analyses show a fascinating picture of conservation and divergence, revealing large blocks of gene orthology with rodents, scattered regions with more recent gene family expansions and deletions, and segments of coding and non-coding conservation with the distant fish species Takifugu.
Asunto(s)
Cromosomas Humanos Par 19/genética , Genes/genética , Mapeo Físico de Cromosoma , Empalme Alternativo/genética , Animales , Composición de Base , Secuencia Conservada/genética , Islas de CpG/genética , Evolución Molecular , Duplicación de Gen , Genética Médica , Humanos , Ratones , Datos de Secuencia Molecular , Familia de Multigenes/genética , Seudogenes/genética , Análisis de Secuencia de ADNRESUMEN
Chromosome 5 is one of the largest human chromosomes and contains numerous intrachromosomal duplications, yet it has one of the lowest gene densities. This is partially explained by numerous gene-poor regions that display a remarkable degree of noncoding conservation with non-mammalian vertebrates, suggesting that they are functionally constrained. In total, we compiled 177.7 million base pairs of highly accurate finished sequence containing 923 manually curated protein-coding genes including the protocadherin and interleukin gene families. We also completely sequenced versions of the large chromosome-5-specific internal duplications. These duplications are very recent evolutionary events and probably have a mechanistic role in human physiological variation, as deletions in these regions are the cause of debilitating disorders including spinal muscular atrophy.
Asunto(s)
Cromosomas Humanos Par 5/genética , Análisis de Secuencia de ADN , Animales , Composición de Base , Cadherinas/genética , Secuencia Conservada/genética , Duplicación de Gen , Genes/genética , Enfermedades Genéticas Congénitas/genética , Genómica , Humanos , Interleucinas/genética , Datos de Secuencia Molecular , Atrofia Muscular Espinal/genética , Pan troglodytes/genética , Mapeo Físico de Cromosoma , Seudogenes/genética , Sintenía/genética , Vertebrados/genéticaRESUMEN
Rhodospirillum rubrum (Esmarch 1887) Molisch 1907 is the type species of the genus Rhodospirillum, which is the type genus of the family Rhodospirillaceae in the class Alphaproteobacteria. The species is of special interest because it is an anoxygenic phototroph that produces extracellular elemental sulfur (instead of oxygen) while harvesting light. It contains one of the most simple photosynthetic systems currently known, lacking light harvesting complex 2. Strain S1(T) can grow on carbon monoxide as sole energy source. With currently over 1,750 PubMed entries, R. rubrum is one of the most intensively studied microbial species, in particular for physiological and genetic studies. Next to R. centenum strain SW, the genome sequence of strain S1(T) is only the second genome of a member of the genus Rhodospirillum to be published, but the first type strain genome from the genus. The 4,352,825 bp long chromosome and 53,732 bp plasmid with a total of 3,850 protein-coding and 83 RNA genes were sequenced as part of the DOE Joint Genome Institute Program DOEM 2002.