Draft genome sequence data of two Salmonella bacteria from serogroup C type.

Salmonella is a gram-negative rod-shape bacterium from the family of Enterobacteriaceae that can cause a wide range of human disease such as enteric fever, gastroenteritis and bacteremia. Here we sequenced two genomes of Salmonella bacteria isolated from the Gallus gallus domesticus host. Genomic DNA of the two Salmonella isolates were extracted and subjected to whole genome sequencing using Illumina platform. The draft genome size of the two Salmonella isolates was determined to be 4,902,295 bp (S18) and 4,847,310 bp (S20) respectively. The percentage of GC content for both draft genomes is the same which is 52.1%. Both the whole genome shotgun project (S18 and S20) has been deposited in National Center for Biotechnology Information Sequence Read Archive under the accession number of SRR7503041 (S18) and SRR7503040 (S20). The sequenced genome (S18 and S20) were aligned with the reference genome and three other Salmonella genomes from serogroup B, D and E. The data obtained show the presence of unique DNA sequences in S18 and S20 genomes. This unique DNA sequences are from the fimbrial gene group.

Genotyping of Salmonella spp. on the basis of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)

@#Aims: The CRISPR locus in Salmonella genome is comprised of three main components which are the (CRISPRassociated) cas genes, an AT-rich leader sequence and the CRISPR array. The length of CRISPR array is determined by the number of spacers within it and varies not only among different organisms but also varies among the bacterial serotypes and strains. This present study aimed at determining if the CRISPR array in Salmonella spp. could be applied to establish a correlation between serogroup type and the fingerprint generated by CRISPR typing. Methodology and results: A total of 30 Salmonella samples were obtained from the Veterinary Diagnostic Laboratory, Kota Kinabalu, Sabah. Salmonella serogroup was determined using the slide agglutination test. Four different serogroups were identified which were serogroup B, C, D, and E. Deoxyribonucleic acid (DNA) was extracted and polymerase chain reaction (PCR) was performed using primers which were designed to amplify the CRISPR array in Salmonella genome. Our results indicate that there is a positive correlation between serogroup results obtained using slide agglutination test and the profile generated by CRISPR typing. Conclusion, significance and impact of study: CRISPR typing has the potential to be applied for the genotyping of Salmonella bacteria.