[Phenotype of blast cells in acute lymphoblastic leukemia]. / Fenotip blastnykh kletok pri ostrom limfoblastnom leikoze.

The complex morphocytochemical, immunological and cytogenetic investigation of the blast cells in acute lymphoblastic leukemia showed their considerable heterogeneity. There were several kinds of cells: typical blast cells, Ph'-positive blast cells expressing only Ia-like antigen and anaplastic blast cells with myeloid and erythroid differentiation.

[The expression of antigens, detectable with ICO-series monoclonal antibodies, on the surfaces of cells forming granulocyte and macrophage colonies in semiliquid agar]. / Ekspressiia antigenov, vyiavliaemykh monoklonal'nymi antitelami serii IKO, na poverkhnosti kletok, obrazuiushchikh granulotsitarnye i makrofagal'nye kolonii v poluzhidkom agare.

The expression of antigens on granulocyte-macrophagal colony-forming cells of patients with nonhematological diseases was studied. Treatment of bone marrow cells with murine monoclonal antibodies ICO-1 and ICO-11 led to statistically significant inhibition of the number of growing colonies. Monoclonal antibodies ICO-02, ICO-10, ICO-GM-1 and ICO-G-2 had no such effect.

[Morphofunctional characteristics of the blast cells in acute myelomonoblastic leukemia]. / Morfofunktsional'nye osobennosti blastnykh kletok pri ostrom mielomonoblastnom leikoze.

Morphocytochemical and immunological characteristics and indices of the cloning effectiveness were studied in agar cultures. Three types of leukemic cells were found whose ratio in the bone marrow varied in a rather wide range. The lymphoid markers (of cOLL and T-antigens) were detected on blast cells. It is established that histogenetic characteristics of the blast cells determines peculiarities of their growth in the agar culture.

[Effect of concanavalin A on the splenic lymphocyte proliferative activity of germ-free rats]. / Vliianie konkanavalina A na proliferativnuiu aktivnost' limfotsitov selezenki bezmikrobnykh krys.

Action of Con-A on the proliferative activity of spleen lymphocytes was studied using germfree and conventional Fisher rats 3-4 months of age and conventional 2-3 day old rats. The intensity of 3H-thymidine incorporation in lymphocytes of germfree and conventional animals was the same, however, the coefficient of stimulation was higher in germfree rats. Lymphocytes of suckling rats had a low reactivity to Con-A.

[Colony formation inhibition in human bone marrow stromal cells exposed to a factor formed in vitro by peripheral blood leukocytes]. / Ingibitsiia obrazovaniia kolonii stromal'nykh kletok chelovecheskogo kostnogo mozga pod vliianiem faktora, obrazuemogo in vitro leikotsitami perifericheskoi krovi

Addition of human peripheral blood leukocytes or a medium in which the leukocytes were cultivated to the monolayer culture of human bone marrow produced an inhibitory action of the growth of the fibroblast colonies. This effect was not associated with the immunological incompatibility of the cells in a culture--autologous blood cells produced the same action as the heterologous ones. The factor inhibiting the fibroblast growth failed to depress the growth of other cells, of macrophages in particular.

[The prognostic significance of pre-existing lymphocytotoxic antibodies in cadaveric kidney allografts with a negative specific crossmatch]. / Prognosticheskoe znachenie predsushchestvuiushchikh limfotsitotoksicheskikh antitel pri allotransplantatsiiakh trupnoi pochki s otritsatel'nym spetsificheskim kross-matchem.

The paper presents the results obtained on preexisting lymphocytotoxic antibodies in 110 patients with the use of cryopreserved donor lymphocytes. Preexisting antibodies (PA) are shown to display in 52% of primary patients in the nephrological department. Follow-up measurements gave evidence on PA levels fluctuations following an undulating form. The analysis of the transplants 3-month survival in the negative cross-match indicated a better prognosis in those patients who showed antibodies to none of the donors. The presence of lymphocytotoxic reactions even of nondonor specificity, on the contrary, made the prognosis much worse.

[Prevention of reduced plasma fibronectin level in acute and chronic phenol and aniline poisoning in rats on vitamin-rich diet]. / Preduprezhdenie snizheniia v plazme krovi urovnia fibronektina pri ostroi i khronicheskoi intoksikatsii fenolom i anilinom u krys, puluchavshikh v ratsione povyshennoe soderzhanie vitaminov.

Plasma fibronectin, a glycoprotein having a great molecular weight and a quaternary structure is a specific animal protein that maintains the body's homeostasis on exposure to various adverse factors, such as mechanical, thermal or chemical injuries, infection, hemorrhage, intoxication or immune disease. In acute phenol intoxication, fibronectin levels were increased in rats up to 680 mg/l during 48 hours after the onset of priming and decreased up to 240 mg/l during the following 48 hours and then became stable. In acute aniline intoxication, fibronectin concentrations were decreased up to 120-130 mg/l during 4 days, later on its level became higher. The mechanisms responsible for the phenomenon are tentatively outlined. This is an acute phase process in the first case (phenol intoxication) and a stress process in the functioning of the reticuloendothelial system in the other case (aniline intoxication).