RESUMEN
The present study aimed to investigate the antiinflammatory effect and mechanism of action of isosecotanapartholide (ISTP), isolated from Artemisia princeps Pampanini extract (APE). The effects of ISTP and APE on the proliferation of human keratinocytes following stimulation by tumor necrosis factorα/interferonγ were assessed. ISTP and APE downregulated the expression levels of signal transducer and activator of transcription1 (STAT1), and reduced interleukin33 (IL33) production. ISTP and APE inhibited the mRNA expression levels of thymus and activationregulated chemokine (TARC/CCL17) in a dosedependent manner. Western blot analysis demonstrated that ISTP and APE dosedependently inhibited protein expression levels of intercellular adhesion molecule1 and phosphorylation of STAT1. The results of the present study indicate that ISTP may inhibit TARC/CCL17 production in human epidermal keratinocytes via the STAT1 signaling pathway and may be associated with the inhibition of IL33 production. The current study indicated that ISTP is an active component in APE and may be a potential therapeutic agent for the treatment of inflammatory skin disorders.