Virtual Planning and Rapid 3D Prototyping Surgical Guide for Anterior Crown Lengthening Surgery: A Clinical Case Report.

Progress with additive 3D printing is revolutionizing biomaterial manufacturing, including clinical dentistry and prosthodontics. Among the several 3D additive printing technologies, stereolithography is very popular as it utilizes light-activated resin for precise resolution. A simplified digital technique was used to fabricate two designs of a surgical guide for crown lengthening. Two cases are presented that utilized digital imaging and communications in medicine (DICOM) files obtained with computed tomography (CT) imaging and processed using four CAD software (Blue Sky Plan, Exocad, Meshmixer and 3D Slicer). The final models were converted to standard tessellation (STL) files and the guides were 3D printed with an additive stereolithography (SLA) printer. The first case was fabricated with a bone model from cone beam computed tomography (CBCT) data, and the second case was generated with intraoral and wax-up scans alone. Both methods appear to be equally effective compared to using a conventional method of guide frabication. However, proximal bone reduction was a concern with both designs. Digitally fabricated 3D printed surgical guide for crown lengthening has merit and a practical design is needed for future clinical validation.

Synergistic effect of hyperbaric oxygen therapy with PTH [1-34] on calvarial bone graft in irradiated rat.

OBJECTIVE: To determine the synergistic effect of parathyroid hormone (PTH) [1-34] in combination with hyperbaric oxygen (HBO) on bone graft in a rat calvarial bone defect model under impaired osteogenic conditions. MATERIALS AND METHODS: Twenty-four rats were divided into three groups. Localized radiation with a single 12 Gy dose was administered to the calvaria. Four weeks after radiation, calvarial circular defects were created in the parietal bones. All defects were filled with biphasic calcium phosphate. After the bone graft, PTH [1-34] was injected subcutaneously, and HBO was administered. At 6 weeks after the bone graft, the rats were sacrificed, and specimens were harvested. RESULTS: Histomorphometric evaluation showed that the percentage of new bone area was higher in the PTH and PTH/HBO groups than in the control group. The percent residual material area was decreased in the PTH/HBO group compared with the control group. The percentage blood vessel number was highest in the PTH group. Micro-CT evaluation showed that the new bone volume was highest in the PTH/HBO group. The residual material volume was lowest in the PTH/HBO group. CONCLUSION: Within the limitations of this study, our data indicate that PTH combined with HBO may reverse radiation-induced impairment of bone healing.

Maxillary implant-retained partial overdenture with Dolder bar attachment: a clinical report.

This article describes a technique for maintaining a maxillary Kennedy III partial removable dental prosthesis design in a patient who had non-restorable failing abutments by replacing the abutments with dental implants. Two implants were placed immediately after extraction of the abutment teeth in the anterior maxilla. After the implants were fully integrated, a Dolder bar attachment was fitted onto the implants. A new maxillary partial removable dental prosthesis was fabricated using the implants and the remaining natural teeth as abutments to restore function and esthetics. With the aid of dental implants, this Kennedy III maxillary removable dental prosthesis design could provide additional retention and support by promoting cross-arch stability and tissue, implant and tooth support. The patient's satisfaction was significantly increased.

Primary stability comparison using piezoelectric or conventional implant site preparation systems in cancellous bone: a pilot study.

PURPOSE: This study compares implant primary stability achieved in cancellous bone after placement in piezoelectric prepared sites versus conventionally drilled sites. MATERIALS AND METHODS: Four bovine ribs were randomly assigned and placed in a water bath at 36.5 °C. Five sites per rib (total n = 20 sites) were prepared using piezoelectric system (test) or conventional drills (control) with twenty 10 × 3.6-mm Implantium implants placed. Using Osstell Mentor quantitative analysis, 5 resonance frequency analysis [implant stability quotient {ISQ}] values per implant were recorded at 5 locations for a total of 100 measurements. RESULTS: Independent t test analysis indicated significant difference in primary stabilities between groups: t (17) = 2.637, P = 0.17, with equal variance assumption satisfied (P = 0.196). Examination of means indicated a higher mean ISQ for piezoelectric than for conventional: 58.9 (+8.55) versus 49.2 (+7.33), respectively. Analysis of variance indicated a significant difference in mean ISQ value by rib. Tukey test indicated significantly higher ISQ values for rib A (test) than ribs B, C (control), and D (test). CONCLUSION: Implant site preparation using the piezoelectric system gives higher implant primary stability in cancellous bone. However, variations in quality across bones may have affected the results.

Strength properties of preceramic brazed joints of a gold-palladium alloy with a microwave-assisted oven and gas/oxygen torch technique.

STATEMENT OF PROBLEM: The effect of microwave brazing on the strength properties of dental casting alloys is not yet known. PURPOSE: The purpose of this study was to compare the strength properties of preceramic brazed joints obtained by using a microwave oven and a conventional torch flame for a high noble alloy (Au-Pd). MATERIAL AND METHODS: A total of 18 tensile bars made of an Au-Pd ceramic alloy were fabricated. Six specimens were cut and joined with a high-fusing preceramic solder in a specially designed microwave oven, and 6 specimens were joined with a conventional natural gas/oxygen torch. The remaining 6 uncut specimens were tested as a control. All the specimens were subjected to testing with a universal testing machine. A 1-way ANOVA was performed for each strength property tested. RESULTS: The tensile strength of the uncut group was the highest (745 ±19 MPa), followed by the microwave group (420 ±68 MPa) and the conventional torch group (348 ±103 MPa) (P<.001); however, no significant difference in tensile strength was found between the microwave group and gas torch group. The tensile strength of the microwave group exceeded ANSI/ADA Standard No. 88, Dental Brazing Alloys (a joint standard of the American National Standards Institute and the American Dental Association). CONCLUSIONS: The microwave heating preceramic solder method demonstrated the excellent tensile strength of an Au-Pd alloy and may be an alternative way of joining alloys when a torch flame is contraindicated.

Effects of soldering methods on tensile strength of a gold-palladium metal ceramic alloy.

STATEMENT OF PROBLEM: The tensile strength obtained by conventional postceramic application soldering and laser postceramic welding may require more energy than microwave postceramic soldering, which could provide similar tensile strength values. PURPOSE: The purpose of the study was to compare the tensile strength obtained by microwave postceramic soldering, conventional postceramic soldering, and laser postceramic welding. MATERIAL AND METHODS: A gold-palladium metal ceramic alloy and gold-based solder were used in this study. Twenty-seven wax specimens were cast in gold-palladium noble metal and divided into 4 groups: laser welding with a specific postfiller noble metal, microwave soldering with a postceramic solder, conventional soldering with the same postceramic solder used in the microwave soldering group, and a nonsectioned control group. All the specimens were heat treated to simulate a normal porcelain sintering sequence. An Instron Universal Testing Machine was used to measure the tensile strength for the 4 groups. The means were analyzed statistically with 1-way ANOVA. The surface and fracture sites of the specimens were subjectively evaluated for fracture type and porosities by using a scanning electron microscope. RESULTS: The mean (standard deviation) ultimate tensile strength values were as follows: nonsectioned control 818 ±30 MPa, microwave 516 ±34 MPa, conventional 454 ±37 MPa, and laser weld 191 ±39 MPa. A 1-way ANOVA showed a significant difference in ultimate tensile strength among the groups (F3,23=334.5; P<.001). Follow-up multiple comparisons showed a significant difference among all the groups. Microwave soldering resulted in a higher tensile strength for gold and palladium noble metals than either conventional soldering or laser welding. CONCLUSION: Conventional soldering resulted in a higher tensile strength than laser welding. Under the experimental conditions described, either microwave or conventional postceramic soldering would appear to satisfy clinical requirements related to tensile strength.

Comparison of fracture resistance of pressable metal ceramic custom implant abutment with a commercially fabricated CAD/CAM zirconia implant abutment.

STATEMENT OF PROBLEM: The predictable nature of the hot pressing ceramic technique has several applications, but no study was identified that evaluated its application to the fabrication of custom implant abutments. PURPOSE: The purpose of this study was to compare the fracture resistance of an experimentally designed pressable metal ceramic custom implant abutment (PR) with that of a duplicate zirconia abutment (ZR). MATERIALS AND METHODS: Two groups of narrow platform (NP) (Nobel Replace) implant abutment specimens were fabricated (n=10). The experimental abutment (PR) had a metal substructure cast with ceramic alloy (Lodestar) and veneered with leucite pressable glass ceramic (InLine PoM). Each PR abutment was individually scanned and 10 duplicate CAD/CAM ZR abutments were fabricated for the control group. Ceramic crowns (n=20) with the average dimensions of a human lateral incisor were pressed with lithium disilicate glass ceramic (IPS e.max Press) and bonded on the abutments with a resin luting agent (Multilink Automix). The specimens were subjected to thermocycling, cyclic loading, and finally static loading to failure with a computer-controlled Universal Testing Machine. An independent t test (1 sided) determined whether the mean values of the fracture load differed significantly (α=.05) between the 2 groups. RESULTS: No specimen failed during cyclic loading. Upon static loading, the mean (SD) load to failure was significantly higher for the PR group (525.89 [143.547] N) than for the ZR group (413.70 [35.515] N) for internal connection narrow platform bone-level implants (P=.025). Failure was initiated at the screw and internal connection level for both groups. CONCLUSIONS: It is possible to fabricate PR abutments that are stronger than ZR abutments for Nobel Biocare internal connection NP bone-level implants. The screw and the internal connection are the weak links for both groups.

Piezoelectric vs. conventional implant site preparation: ex vivo implant primary stability.

OBJECTIVES: This study aims to determine differences in primary stability between implants placed in cortical bone following Piezoelectric or conventional site preparation, as assessed by resonance frequency analysis (RFA) and reverse torque testing (RTT). MATERIAL AND METHODS: Four fresh bovine ribs were acquired and surgical guides fabricated with five sites per rib (n = 20), for proper site preparation. Implant sites were prepared via conventional drilling technique as per manufacturer's instruction (Implantium) or via Piezoelectric (Mectron) implant site preparation using the Implant Prep kit. Twenty 10 mm long, 3.6 mm diameter Implantium implants were placed with 35 Ncm torque; 10 implants per preparation method. RFA was assessed via the Osstell Mentor. Five values were taken per implant. All implants where subjected to a reverse torque in increasing increments of 5 until 50 Ncm force was reached. RESULTS: The five RFA values per site were averaged and plotted by placement technique. A paired t-test statistical analysis was run. The average RFA values showed no statistical significance between the 10 test (RFA = 69.04 ± 5.11) and 10 control (RFA = 70.94 ± 6.41) sites (P > 0.05). All implants in both groups withstood RTT up to 50 Ncm force without movement and thus showed no statistical differences. CONCLUSION: Results of this ex vivo study imply that the Piezoelectric implant site preparation affords similar primary implant stability in comparison to conventional rotary instrumentation in cortical bone.

Applying microwave technology to sintering dental zirconia.

STATEMENT OF PROBLEM: When sintering zirconia, conventional processing may not provide uniform heating and consumes more energy than an alternative method using microwave energy. PURPOSE: The purpose of this study was to compare the surface quality, mechanical and physical properties, and dimensional stability obtained by sintering yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) in a conventional furnace versus a microwave furnace. MATERIAL AND METHODS: Twenty bars of Y-TZP were prepared from Zircad blocks. Ten specimens were used for sintering in a conventional furnace. The remaining 10 specimens were sintered in a microwave furnace. The sintering temperature used for both techniques was 1500°C. The flexural strength of all specimens was measured with the 3-point bend test with a universal testing machine with a cross head speed of 1.0 mm/min. Density was measured by applying the Archimedes method, and specimen length, width, and thickness were measured with a digital micrometer. The phase composition and average grain size of these ceramics were examined by using X-ray diffraction, and microstructure characteristics were studied with scanning electron microscopy. Data obtained were analyzed by using independent t tests (α=.05). RESULTS: No significant difference between conventional and microwave sintering for either flexural strength, t18=0.49 (P=.63) or density, t18=0.07 (P=.95) was found. Specimens in both groups exhibited a uniform firing shrinkage of approximately 24.6% in all dimensions. The surface of selected specimens examined with a scanning electron microscope showed no visible difference in grain shape or porosity size between the 2 sintering methods. CONCLUSIONS: Under the conditions of this study, it appears that either microwave or conventional zirconia sintering may be used for processing zirconia for dental use. However, microwave energy provides uniformity of heating, allowing the use of higher heating rates, which can increase productivity and save energy.

Internally weighted mandibular denture fabrication using a processed denture base.

This article describes a method of fabricating a customized weighted metal base for the mandibular complete denture using a processed denture base and a plaster index of the tooth arrangement.

Comparison of porcelain surface and flexural strength obtained by microwave and conventional oven glazing.

STATEMENT OF PROBLEM: Although the superior qualities of microwave technology are common knowledge in the industry, effects of microwave glazing of dental ceramics have not been investigated. PURPOSE: The purpose of this study was to investigate the surface roughness and flexural strength achieved by glazing porcelain specimens in a conventional and microwave oven. MATERIAL AND METHODS: Thirty specimens of each type of porcelain (Omega 900 and IPS d.Sign) were fabricated and sintered in a conventional oven. The specimens were further divided into 3 groups (n=10): hand polished (using diamond rotary ceramic polishers), microwave glazed, and conventional oven glazed. Each specimen was evaluated for surface roughness using a profilometer. The flexural strength of each specimen was measured using a universal testing machine. A 2-way ANOVA and Tukey HSD post hoc analysis were used to determine significant intergroup differences in surface roughness (alpha=.05). Flexural strength results were also analyzed using 2-way ANOVA, and the Weibull modulus was determined for each of the 6 groups. The surfaces of the specimens were subjectively evaluated for cracks and porosities using a scanning electron microscope (SEM). RESULTS: A significant difference in surface roughness was found among the surface treatments (P=.02). Follow-up tests showed a significant difference in surface roughness between oven-glazed and microwave-glazed treatments (P=.02). There was a significant difference in flexural strength between the 2 porcelains (P<.005), but no significant difference in flexural strength by surface treatment (P=.48). The Weibull modulus value for the Omega 900 microwave-glazed group was the highest (1.9) as compared to the other groups. CONCLUSIONS: The surface character of microwave-glazed porcelain was superior to oven-glazed porcelain. Omega 900 had an overall higher flexural strength than IPS d.Sign. Weibull distributions of flexural strengths for Omega 900 oven-glazed and microwave-glazed specimens were similar. SEM analysis demonstrated a greater number of surface voids and imperfections in IPS d. Sign as compared to Omega 900.

Comparison of fracture resistance of pressable metal ceramic custom implant abutments with CAD/CAM commercially fabricated zirconia implant abutments.

STATEMENT OF PROBLEM: The adequacy of the strength of dental zirconia abutments under heavy occlusal load conditions is questionable, with degradation of the mechanical properties of zirconia having been reported. Therefore, some alternatives must be considered. PURPOSE: The purpose of this study was to determine the efficacy of fabricating pressable metal ceramic custom implant (Pr) abutments, and to evaluate the fracture resistance of these abutments. MATERIAL AND METHODS: Two groups of implant abutment specimens were fabricated (n=10). The experimental group consisted of Pr abutments, and the control group consisted of CAD/CAM-designed zirconia-based ceramic (Zr) abutments. For the experimental group, custom metal abutments were cast using a compatible metal alloy (Lodestar); this was followed by injection molding with lithium disilicate pressable ceramic (IPS e.max Press) around the metal column of the custom implant abutment. For the control group, 10 specimens were fabricated from CAD/CAM-designed zirconia abutments (Procera Zirconia). Following scanning, all-ceramic crowns with the average dimensions of a human central incisor were fabricated for the experimental and control abutments (n=20) using lithium disilicate pressable ceramic (IPS e.max Press). Each crown was cemented to the implant abutments with a resin luting agent (Variolink II). The crown-abutment test specimens were fixed to titanium implant analogs and placed in a test stand at 30 degrees from the vertical axis of the specimens in a computer-controlled universal testing device. The independent t test was used to detect if the mean values of the fracture load differed significantly (alpha=.05) between the 2 groups. RESULTS: The mean (SD) fracture load was significantly higher in the Pr group (901.67 (102.05) N) than in the Zr group (480.01 (174.46) N, P<.005). CONCLUSIONS: This study found that Pr abutments are stronger than Zr abutments.

Periodontal biotype modification using a volume-stable collagen matrix and autogenous subepithelial connective tissue graft for the treatment of gingival recession: a case series.

PURPOSE: The purpose of this study was to propose a technique for periodontal biotype modification through thickening of the entire facial aspect using a volume-stable collagen matrix and autogenous subepithelial connective tissue graft (CTG) for the treatment of gingival recession. METHODS: Four systemically healthy patients showing Miller class I or class II gingival recession in the mandibular incisor area were included in this study. Full-mouth scaling and root planing procedures were performed at least 4 weeks prior to periodontal plastic surgery. A split-thickness flap with a horizontal intrasulcular incision and 2 vertical incisions was used in cases 1-3, and the modified tunnel technique was used in case 4 for coronal advancement of the mucogingival complex. After the exposed root surfaces were debrided thoroughly, double-layered volume-stable collagen matrix was placed on the apical part of the recession and a subepithelial CTG harvested from the palatal area was placed on the coronal part. The amount of root coverage at 3 months postoperatively was evaluated in cases 1-3, and facio-lingual volumetric changes were analyzed in cases 1 and 2. RESULTS: Healing was uneventful in all 4 cases and complete root coverage was shown in cases 1-3. In case 4, reduction of gingival recession was observed at 3 months after surgery. In cases 1 and 2, a comparison of stereolithographic files from the preoperative and postoperative time points demonstrated that the entire facio-lingual volume had increased. CONCLUSIONS: The surgical technique suggested herein, using a volume-stable collagen matrix and autogenous subepithelial CTG, may be an effective method for periodontal biotype modification through thickening of the entire facial aspect for the treatment of gingival recession.

Ridge preservation using basic fibroblast growth factor-2 and collagenated biphasic calcium phosphate in beagle dogs.

PURPOSE: The aim of this study was to evaluate volumetric and histologic changes in edentulous alveolar ridge areas after ridge preservation using basic fibroblast growth factor-2 (bFGF-2) in combination with collagenated biphasic calcium phosphate (BCP). METHODS: The experiments were performed in 6 adult male beagle dogs. The following 3 groups were created: 1) ridge preservation with bFGF-2 and collagenated BCP (experimental group), 2) ridge preservation with collagenated BCP (positive control group), and 3) a negative control group in which no ridge preservation procedure was performed. Volumetric change analysis was performed using an optical scanner and casts. Histological observations were made using light microscopy. RESULTS: After the initial swelling subsided, the magnitude of the volumetric change in the experimental group and positive control group was smaller than in the negative control group. In the experimental group, a distinct trend was observed for the resorption of residual bone and collagen fibers at 4 weeks and for more mature bone and faster healing at 12 weeks. CONCLUSIONS: Based on the findings of the present study, bFGF-2 may be considered for use as a therapeutic molecule in ridge preservation procedures.

Composite Electrolyte for All-Solid-State Lithium Batteries: Low-Temperature Fabrication and Conductivity Enhancement.

All-solid-state lithium batteries offer notable advantages over conventional Li-ion batteries with liquid electrolytes in terms of energy density, stability, and safety. To realize this technology, it is critical to develop highly reliable solid-state inorganic electrolytes with high ionic conductivities and adequate processability. Li1+x Alx Ti2-x (PO4 )3 (LATP) with a NASICON (Na superionic conductor)-like structure is regarded as a potential solid electrolyte, owing to its high "bulk" conductivity (ca. 10-3  S cm-1 ) and excellent stability against air and moisture. However, the solid LATP electrolyte still suffers from a low "total" conductivity, mainly owing to the blocking effect of grain boundaries to Li+ conduction. In this study, an LATP-Bi2 O3 composite solid electrolyte shows very high total conductivity (9.4×10-4  S cm-1 ) at room temperature. Bi2 O3 acts as a microstructural modifier to effectively reduce the fabrication temperature of the electrolyte and to enhance its ionic conductivity. Bi2 O3 promotes the densification of the LATP electrolyte, thereby improving its structural integrity, and at the same time, it facilitates Li+ conduction, leading to reduced grain-boundary resistance. The feasibility of the LATP-Bi2 O3 composite electrolyte in all-solid-state Li batteries is also examined in this study.

Prosthetic failure in implant dentistry.

Although osseointegrated dental implants have become a predictable and effective modality for the treatment of single or multiple missing teeth, their use is associated with clinical complications. Such complications can be biologic, technical, mechanical, or esthetic and may compromise implant outcomes to various degrees. This article presents prosthetic complications accompanied with implant-supported single and partial fixed dental prostheses.

Size-dependent strength of dental adhesive systems.

OBJECTIVE: The aim of this study is to explain the influence of peripheral interface stress singularities on the testing of tensile bond strength. The relationships between these theoretically predicted singularities and the effect of specimen size on the measured bond strength are evaluated. METHODS: Finite element method (FEM) and boundary element method (BEM) analyses of microtensile bond strength test specimens were performed and the presence of localized high stress concentrations and singularities was analyzed. The specimen size effect predicted by the models was compared to previously published experimental data. RESULTS: FEM analysis of single-material trimmed hour-glass versus cast cylindrical specimens showed different theoretical stress distributions, with the dumbbell or cylindrical specimens showing a more homogeneous distribution of the stress on the critical symmetry plane. For multi-material specimens, mathematical singularities at the free edge of the bonded interface posed a computational challenge that resulted in mesh-dependence in the standard FEM analysis. A specialized weighted-traction BEM analysis, designed to eliminate mesh-dependence by capturing the effect of the singularity, predicted a specimen size effect that corresponds to that published previously in the literature. SIGNIFICANCE: The results presented here further support the attention to specimen dimensions that has already broadened the empirical use of the microtensile test methods. FEM and BEM analyses that identify stress concentrations and especially marginal stress singularities must be accounted for in reliable bonding strength assessments. Size-dependent strength variations generally attributed to the effects of flaw distributions throughout the interfacial region are not as relevant as the presence of singularities at bonded joint boundaries - as revealed by both FEM and BEM analyses, when interpreted from a generalized fracture mechanics perspective. Furthermore, this size-dependence must be considered when evaluating or designing dental adhesive systems.

Should anticoagulants be administered for portal vein thrombosis associated with acute pancreatitis?

Venous complications in patients with acute pancreatitis typically occur as a form of splenic, portal, or superior mesenteric vein thrombosis and have been detected more frequently in recent reports. Although a well-organized protocol for the treatment of venous thrombosis has not been established, anticoagulation therapy is commonly recommended. A 73-year-old man was diagnosed with acute progressive portal vein thrombosis associated with acute pancreatitis. After one month of anticoagulation therapy, the patient developed severe hematemesis. With endoscopy and an abdominal computed tomography scan, hemorrhages in the pancreatic pseudocyst, which was ruptured into the duodenal bulb, were confirmed. After conservative treatment, the patient was stabilized. While the rupture of a pseudocyst into the surrounding viscera is a well-known phenomenon, spontaneous rupture into the duodenum is rare. Moreover, no reports of upper gastrointestinal bleeding caused by pseudocyst rupture in patients under anticoagulation therapy for venous thrombosis associated with acute pancreatitis have been published. Herein, we report a unique case of massive upper gastrointestinal bleeding due to pancreatic pseudocyst rupture into the duodenum, which developed during anticoagulation therapy for portal vein thrombosis associated with acute pancreatitis.

Enhanced bone forming ability of SLA-treated Ti coated with a calcium phosphate thin film formed by e-beam evaporation.

With an electron-beam evaporation process, a calcium phosphate (Ca-P) thin film of approximately 500 nm thick was deposited on sand blasted with large grits and acid etched (SLA) Ti without changing the typical morphology of the SLA surface. Dissolution behavior was investigated by measuring the amount of dissolved phosphate ions with ion chromatography after immersing the SLA Ti sample coated with a Ca-P film in 1 ml de-ionized water maintained at 37 degrees C for different periods of soaking time, and the surface morphology was observed with field emission scanning electron microscopy. The amount of phosphate ions increased quickly right after immersion but began to decrease after 2 days of immersion by redeposition with Ca ions as apatite, and the amount of biomimetic apatite increased with the extended soaking time. The Saos-2 cell was more attached on the coated surface, and the in vivo evaluation was that the Ca-P deposited SLA implant greatly improved the new bone formation ability.

Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition.

Technical development for an efficient coating of bioactive materials improves the characteristics of a fully functional implant. The aim of this study was to investigate the osteoinductive effect of a newly developed hydroxyapatite (HA)-coating technique using aerosol deposition without post-heat treatment [room temperature (RT) group] on the titanium (Ti) dental implant in vitro and in vivo, compared with that of HA coating with post-heat treatment (HT-400 group) or machined surface (control group). Cell proliferation or attachment on the HA-coated Ti surface was assessed using tetrazolium salt, WST-8 or scanning electron microscopy (SEM). Human osteoblasts (HOB) on RT group were well attached and grew alike in the control or HT-400 group. The alkaline phosphatase activity of HOB cultured on RT and HT-400 group was significantly higher than the control group (p < 0.05). Evaluation by SEM, TEM, and XRD demonstrated that aerosol deposition facilitated HA particles to form a dense and uniform HA layer in the RT group despite no post-heating. In a rabbit tibia model (n = 3), the ratios of bone implant contact and bone area in the RT group (49.88%, 86.05%) were greater than in the HT-400 group (38.82%, 77.34%) or the control (28.31%, 73.86%). The finding of this study showed that the HA coating using aerosol deposition without post-heat treatment has a good biocompatibility, and provide a promoting strategy to enhance osseointegration in the application of the dental implant.