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1.
Proc Natl Acad Sci U S A ; 114(12): E2466-E2475, 2017 03 21.
Artículo en Inglés | MEDLINE | ID: mdl-28265061

RESUMEN

Ribonucleoprotein (RNP) granules are enriched in specific RNAs and RNA-binding proteins (RBPs) and mediate critical cellular processes. Purified RBPs form liquid droplets in vitro through liquid-liquid phase separation and liquid-like non-membrane-bound structures in cells. Mutations in the human RBPs TAR-DNA binding protein 43 (TDP-43) and RNA-binding protein FUS cause amyotrophic lateral sclerosis (ALS), but the biophysical properties of these proteins have not yet been studied in neurons. Here, we show that TDP-43 RNP granules in axons of rodent primary cortical neurons display liquid-like properties, including fusion with rapid relaxation to circular shape, shear stress-induced deformation, and rapid fluorescence recovery after photobleaching. RNP granules formed from wild-type TDP-43 show distinct biophysical properties depending on axonal location, suggesting maturation to a more stabilized structure is dependent on subcellular context, including local density and aging. Superresolution microscopy demonstrates that the stabilized population of TDP-43 RNP granules in the proximal axon is less circular and shows spiculated edges, whereas more distal granules are both more spherical and more dynamic. RNP granules formed by ALS-linked mutant TDP-43 are more viscous and exhibit disrupted transport dynamics. We propose these altered properties may confer toxic gain of function and reflect differential propensity for pathological transformation.


Asunto(s)
Esclerosis Amiotrófica Lateral/genética , Gránulos Citoplasmáticos/metabolismo , Proteínas de Unión al ADN/genética , Neuronas Motoras/metabolismo , Ribonucleoproteínas/metabolismo , Esclerosis Amiotrófica Lateral/metabolismo , Animales , Axones/química , Axones/metabolismo , Células Cultivadas , Gránulos Citoplasmáticos/química , Gránulos Citoplasmáticos/genética , Proteínas de Unión al ADN/metabolismo , Humanos , Neuronas Motoras/química , Mutación , Ratas , Ratas Sprague-Dawley , Ribonucleoproteínas/genética , Viscosidad
2.
Traffic ; 18(12): 808-824, 2017 12.
Artículo en Inglés | MEDLINE | ID: mdl-28941293

RESUMEN

The unique polarization of neurons depends on selective sorting of axonal and somatodendritic cargos to their correct compartments. Axodendritic sorting and filtering occurs within the axon initial segment (AIS). However, the underlying molecular mechanisms responsible for this filter are not well understood. Here, we show that local activation of the neuronal-specific kinase cyclin-dependent kinase 5 (CDK5) is required to maintain AIS integrity, as depletion or inhibition of CDK5 induces disordered microtubule polarity and loss of AIS cytoskeletal structure. Furthermore, CDK5-dependent phosphorylation of the dynein regulator Ndel1 is required for proper re-routing of mislocalized somatodendritic cargo out of the AIS; inhibition of this pathway induces profound mis-sorting defects. While inhibition of the CDK5-Ndel1-Lis1-dynein pathway alters both axonal microtubule polarity and axodendritic sorting, we found that these defects occur on distinct timescales; brief inhibition of dynein disrupts axonal cargo sorting before loss of microtubule polarity becomes evident. Together, these studies identify CDK5 as a master upstream regulator of trafficking in vertebrate neurons, required for both AIS microtubule organization and polarized dynein-dependent sorting of axodendritic cargos, and support an ongoing and essential role for dynein at the AIS.


Asunto(s)
Segmento Inicial del Axón/metabolismo , Axones/metabolismo , Quinasa 5 Dependiente de la Ciclina/metabolismo , Dineínas/metabolismo , Neuronas/metabolismo , Animales , Células Cultivadas , Citoesqueleto/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Microtúbulos/metabolismo , Transporte de Proteínas/fisiología , Ratas Sprague-Dawley
3.
Mol Cell Neurosci ; 92: 1-11, 2018 10.
Artículo en Inglés | MEDLINE | ID: mdl-29936143

RESUMEN

E2F1 is a transcription factor classically known to regulate G0/G1 to S phase progression in the cell cycle. In addition, E2F1 also regulates a wide range of apoptotic genes and thus has been well studied in the context of neuronal death and neurodegenerative diseases. However, its function and regulation in the mature central nervous system are not well understood. Alternative splicing is a well-conserved post-transcriptional mechanism common in cells of the CNS and is necessary to generate diverse functional modifications to RNA or protein products from genes. Heretofore, physiologically significant alternatively spliced E2F1 transcripts have not been reported. In the present study, we report the identification of two novel alternatively spliced E2F1 transcripts: E2F1b, an E2F1 transcript retaining intron 5, and E2F1c, an E2F1 transcript excluding exon 6. These alternatively spliced transcripts are observed in the brain and neural cell types including neurons, astrocytes, and undifferentiated oligodendrocytes. The expression of these E2F1 transcripts is distinct during maturation of primary hippocampal neuroglial cells. Pharmacologically-induced global translation inhibition with cycloheximide, anisomycin or thapsigargin lead to significantly reduced expression of E2F1a, E2F1b and E2F1c. Conversely, increasing neuronal activity by elevating the concentration of potassium chloride selectively increased the expression of E2F1b. Furthermore, experiments expressing these variants in vitro show the transcripts can be translated to generate a protein product. Taken together, our data suggest that the alternatively spliced E2F1 transcript behave differently than the E2F1a transcript, and our results provide a foundation for future investigation of the function of E2F1 splice variants in the CNS.


Asunto(s)
Empalme Alternativo , Factor de Transcripción E2F1/genética , Hipocampo/metabolismo , Animales , Células Cultivadas , Factor de Transcripción E2F1/metabolismo , Hipocampo/citología , Neuroglía/metabolismo , Neuronas/metabolismo , Ratas , Ratas Sprague-Dawley
4.
Neurol Genet ; 10(2): e200128, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38486676

RESUMEN

Objectives: Characterize the presentation, workup, and management of SGCE myoclonus-dystonia, a rare genetic condition, in a patient with atypical presenting symptoms and no family history of movement abnormalities. Methods: A woman with myoclonus and dystonia was identified based on clinical history and physical examination. Workup was conducted to determine the cause of her symptoms, including whole-exome sequencing. Myoclonus-dystonia is associated with more than 100 distinct mutations in MYC/DYT-SGCE that account for only half of the total myoclonus-dystonia patients. As such, this case required intensive genetic analyses rather than screening only for a small subset of well-characterized mutations. Results: Childhood onset myoclonus and worsening dystonia with age were identified in a young woman. She underwent screening for common causes of twitching movements, followed by whole-exome sequencing which identified a de novo novel variant in the SGCE gene, resulting in a diagnosis of SGCE myoclonus-dystonia. Discussion: Myoclonus-dystonia should be considered in patients with symptoms of head and upper extremity myoclonus early in life, especially with co-occurring dystonia, even in the absence of a family history of similar symptoms. Diagnosis of this condition should take place using sequencing, as new mutations continue to be discovered.

5.
FASEB J ; 22(12): 4281-95, 2008 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-18757500

RESUMEN

Metalloproteinase cleavage of transmembrane proteins (ectodomain cleavage), including the epidermal growth factor (EGF) ligands heparin-binding EGF-like growth factor (HB-EGF), neuregulin (NRG), and transforming growth factor-alpha (TGF-alpha), is important in many cellular signaling pathways and is disregulated in many diseases. It is largely unknown how physiological stimuli of ectodomain cleavage--hypertonic stress, phorbol ester, or activation of G-protein-coupled receptors [e.g., by lysophosphatidic acid (LPA)]--are molecularly connected to metalloproteinase activation. To study this question, we developed a fluorescence-activated cell sorting (FACS)- based assay that measures cleavage of EGF ligands in single living cells. EGF ligands expressed in mouse lung epithelial cells are differentially and specifically cleaved depending on the stimulus. Inhibition of protein kinase C (PKC) isoenzymes or metalloproteinase inhibition by batimastat (BB94) showed that different regulatory signals are used by different stimuli and EGF substrates, suggesting differential effects that act on the substrate, the metalloproteinase, or both. For example, hypertonic stress led to strong cleavage of HB-EGF and NRG but only moderate cleavage of TGF-alpha. HB-EGF, NRG, and TGF-alpha cleavage was not dependent on PKC, and only HB-EGF and NRG cleavage were inhibited by BB94. In contrast, phorbol 12-myristate-13-acetate (TPA) -induced cleavage of HB-EGF, NRG, and TGF-alpha was dependent on PKC and sensitive to BB94 inhibition. LPA led to significant cleavage of only NRG and TGF-alpha and was inhibited by BB94; only LPA-induced NRG cleavage required PKC. Surprisingly, specific inhibition of atypical PKCs zeta and iota [not activated by diacylglycerol (DAG) and calcium] significantly enhanced TPA-induced NRG cleavage. Employed in a high-throughput cloning strategy, our cleavage assay should allow the identification of candidate proteins involved in signal transduction of different extracellular stimuli into ectodomain cleavage.


Asunto(s)
Péptidos y Proteínas de Señalización Intercelular/metabolismo , Proteínas de la Membrana/metabolismo , Metaloproteasas/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Proteína Quinasa C/metabolismo , Factor de Crecimiento Transformador alfa/metabolismo , Animales , Células Cultivadas , Citometría de Flujo/métodos , Factor de Crecimiento Similar a EGF de Unión a Heparina , Humanos , Indoles/farmacología , Isoenzimas/metabolismo , Maleimidas/farmacología , Metaloproteasas/antagonistas & inhibidores , Ratones , Neurregulina-1 , Fenilalanina/análogos & derivados , Fenilalanina/farmacología , Proteína Quinasa C/antagonistas & inhibidores , Tiofenos/farmacología
6.
Acad Pathol ; 6: 2374289519848353, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31206012

RESUMEN

Molecular profiling of glioblastoma has revealed complex cytogenetic, epigenetic, and molecular abnormalities that are necessary for diagnosis, prognosis, and treatment. Our neuro-oncology group has developed a data-driven, institutional consensus guideline for efficient and optimal workup of glioblastomas based on our routine performance of molecular testing. We describe our institution's testing algorithm, assay development, and genetic findings in glioblastoma, to illustrate current practices and challenges in neuropathology related to molecular and genetic testing. We have found that coordination of test requisition, tissue handling, and incorporation of results into the final pathologic diagnosis by the neuropathologist improve patient care. Here, we present analysis of O6-methylguanine-DNA-methyltransferase promoter methylation and next-generation sequencing results of 189 patients, obtained utilizing our internal processes led by the neuropathology team. Our institutional pathway for neuropathologist-driven molecular testing has streamlined the management of glioblastoma samples for efficient return of results for incorporation of genomic data into the pathological diagnosis and optimal patient care.

7.
Trends Neurosci ; 41(9): 555-556, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-30143179

RESUMEN

Active intracellular transport of organelles relies on the coordinated activities of cytoplasmic dynein and kinesin, ATP-dependent microtubule motor proteins. While axonemal dynein was discovered during the mid-1960s, it was not until the mid-1980s that kinesin was discovered by Ron Vale and colleagues, as reported in 1985. Their research demonstrated that the newly identified protein, isolated from both squid axoplasm and bovine brain, was independently capable of driving microtubule gliding or organelle movement. These findings kicked off rapid progress in the fields of physiology and neuroscience, leading to the identification of the many members of the extended kinesin superfamily, as well as detailed explorations of their biophysical properties, cellular mechanisms of action, and roles in disease.


Asunto(s)
Cinesinas/fisiología , Animales , Transporte Biológico , Dineínas/fisiología , Humanos , Orgánulos/fisiología
8.
Methods Cell Biol ; 131: 409-24, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26794526

RESUMEN

Axonal transport is important for neuronal development and the maintenance of effective neuronal function in mature cells. Observing the active transport of organelles and vesicles along the axons of living neurons has emerged as a valuable tool for probing the health of the neuron, and assessing changes associated with stress and neurodegenerative disease. Transport relies on two families of motor proteins: kinesins and dynein. Using these motors, a diverse set of cargos are transported toward the axon tip, the cell body, or anywhere in between. Of particular interest are organelles and cargos associated with disease and the changes in motility that these cargos undergo during pathogenesis. Here, we describe the factors that should be considered when studying different cargos, and the imaging parameters associated with optimal tracking of various organelles and proteins. Ultimately, the ideal cargo to investigate depends on the question being asked and the limitations of individual microscopes available for imaging.


Asunto(s)
Transporte Axonal/fisiología , Axones/metabolismo , Dendritas/metabolismo , Ganglios Espinales/metabolismo , Hipocampo/metabolismo , Animales , Células Cultivadas , Dineínas Citoplasmáticas/metabolismo , Ganglios Espinales/citología , Hipocampo/citología , Cinesinas/metabolismo , Quimografía/métodos , Proteína 1 de la Membrana Asociada a los Lisosomas/metabolismo , Ratones , Proteínas Asociadas a Microtúbulos/metabolismo , Mitocondrias/metabolismo , Cultivo Primario de Células , Ratas , Receptor trkB/metabolismo , Proteínas de Unión al GTP rab/metabolismo , Proteínas de Unión a GTP rab7
9.
Cell Rep ; 12(3): 462-73, 2015 Jul 21.
Artículo en Inglés | MEDLINE | ID: mdl-26166569

RESUMEN

Axonal transport is essential for neuronal function, and defects in transport are associated with multiple neurodegenerative diseases. Aberrant cyclin-dependent kinase 5 (CDK5) activity, driven by the stress-induced activator p25, also is observed in these diseases. Here we show that elevated CDK5 activity increases the frequency of nonprocessive events for a range of organelles, including lysosomes, autophagosomes, mitochondria, and signaling endosomes. Transport disruption induced by aberrant CDK5 activation depends on the Lis1/Ndel1 complex, which directly regulates dynein activity. CDK5 phosphorylation of Ndel1 favors a high affinity Lis1/Ndel/dynein complex that blocks the ATP-dependent release of dynein from microtubules, inhibiting processive motility of dynein-driven cargo. Similar transport defects observed in neurons from a mouse model of amyotrophic lateral sclerosis are rescued by CDK5 inhibition. Together, these studies identify CDK5 as a Lis1/Ndel1-dependent regulator of transport in stressed neurons, and suggest that dysregulated CDK5 activity contributes to the transport deficits observed during neurodegeneration.


Asunto(s)
1-Alquil-2-acetilglicerofosfocolina Esterasa/metabolismo , Transporte Axonal/fisiología , Proteínas Portadoras/metabolismo , Quinasa 5 Dependiente de la Ciclina/metabolismo , Dineínas/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , 1-Alquil-2-acetilglicerofosfocolina Esterasa/genética , Esclerosis Amiotrófica Lateral/metabolismo , Animales , Proteínas Portadoras/genética , Quinasa 5 Dependiente de la Ciclina/genética , Modelos Animales de Enfermedad , Endosomas/metabolismo , Células HeLa , Humanos , Lisosomas/metabolismo , Ratones , Proteínas Asociadas a Microtúbulos/genética , Neuronas/metabolismo
10.
Invest Ophthalmol Vis Sci ; 55(10): 6381-97, 2014 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-25190651

RESUMEN

PURPOSE: We characterized retinal structure in patients and carriers of choroideremia using adaptive optics and other high resolution modalities. METHODS: A total of 57 patients and 18 carriers of choroideremia were imaged using adaptive optics scanning light ophthalmoscopy (AOSLO), optical coherence tomography (OCT), autofluorescence (AF), and scanning light ophthalmoscopy (SLO). Cone density was measured in 59 eyes of 34 patients where the full cone mosaic was observed. RESULTS: The SLO imaging revealed scalloped edges of RPE atrophy and large choroidal vessels. The AF imaging showed hypo-AF in areas of degeneration, while central AF remained present. OCT images showed outer retinal tubulations and thinned RPE/interdigitation layers. The AOSLO imaging revealed the cone mosaic in central relatively intact retina, and cone density was either reduced or normal at 0.5 mm eccentricity. The border of RPE atrophy showed abrupt loss of the cone mosaic at the same location. The AF imaging in comparison with AOSLO showed RPE health may be compromised before cone degeneration. Other disease features, including visualization of choroidal vessels, hyper-reflective clumps of cones, and unique retinal findings, were tabulated to show the frequency of occurrence and model disease progression. CONCLUSIONS: The data support the RPE being one primary site of degeneration in patients with choroideremia. Photoreceptors also may degenerate independently. High resolution imaging, particularly AOSLO in combination with OCT, allows single cell analysis of disease in choroideremia. These modalities promise to be useful in monitoring disease progression, and in documenting the efficacy of gene and cell-based therapies for choroideremia and other diseases as these therapies emerge. (ClinicalTrials.gov number, NCT01866371.).


Asunto(s)
Coroideremia/diagnóstico , Técnicas de Diagnóstico Oftalmológico/instrumentación , Óptica y Fotónica , Células Fotorreceptoras Retinianas Conos/patología , Tomografía de Coherencia Óptica/instrumentación , Adolescente , Adulto , Estructuras Celulares , Niño , Coroideremia/fisiopatología , Diseño de Equipo , Angiografía con Fluoresceína/instrumentación , Estudios de Seguimiento , Fondo de Ojo , Humanos , Persona de Mediana Edad , Oftalmoscopios , Oftalmoscopía/métodos , Reproducibilidad de los Resultados , Agudeza Visual , Adulto Joven
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