RESUMEN
Action of Con-A on the proliferative activity of spleen lymphocytes was studied using germfree and conventional Fisher rats 3-4 months of age and conventional 2-3 day old rats. The intensity of 3H-thymidine incorporation in lymphocytes of germfree and conventional animals was the same, however, the coefficient of stimulation was higher in germfree rats. Lymphocytes of suckling rats had a low reactivity to Con-A.
Asunto(s)
Concanavalina A/farmacología , Vida Libre de Gérmenes/efectos de los fármacos , Linfocitos/efectos de los fármacos , Bazo/citología , Animales , Animales Recién Nacidos , División Celular/efectos de los fármacos , Femenino , Activación de Linfocitos/efectos de los fármacos , Masculino , RatasRESUMEN
The modified method for obtaining encephalitogenic protein from the brain of a guinea pig with the use of gel filtration and ion-exchange chromatography is described. The electrophoretic mobility of the isolated protein is characterized and its molecular weight is determined.
Asunto(s)
Química Encefálica , Proteínas de la Mielina/aislamiento & purificación , Animales , Cromatografía en Gel/métodos , Cromatografía por Intercambio Iónico/métodos , Electroforesis en Gel de Poliacrilamida/métodos , CobayasRESUMEN
Immunochemically pure IgE was obtained from the serum of LOW rats carrying IgE-plasmocytome by the immunochemical methods (ion-exchange chromatography and gel filtration). This IgE served as an antigen for immunization of a rabbit to produce a specific antiserum. The monospecificity of this antiserum was achieved by its sorption with normal serum proteins.