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Microglia play key roles in brain homeostasis as well as responses to neurodegeneration and neuroinflammatory processes caused by physical disease and psychosocial stress. The pig is a physiologically relevant model species for studying human neurological disorders, many of which are associated with microglial dysfunction. Furthermore, pigs are an important agricultural species, and there is a need to understand how microglial function affects their welfare. As a basis for improved understanding to enhance biomedical and agricultural research, we sought to characterize pig microglial identity at genome-wide scale and conduct inter-species comparisons. We isolated pig hippocampal tissue and microglia from frontal cortex, hippocampus, and cerebellum, as well as alveolar macrophages from the lungs and conducted RNA-sequencing (RNAseq). By comparing the transcriptomic profiles between microglia, macrophages, and hippocampal tissue, we derived a set of 239 highly enriched genes defining the porcine core microglial signature. We found brain regional heterogeneity based on 150 genes showing significant (adjusted p < 0.01) regional variations and that cerebellar microglia were most distinct. We compared normalized gene expression for microglia from human, mice and pigs using microglia signature gene lists derived from each species and demonstrated that a core microglial marker gene signature is conserved across species, but that species-specific expression subsets also exist. Our data provide a valuable resource defining the pig microglial transcriptome signature that validates and highlights pigs as a useful large animal species bridging between rodents and humans in which to study the role of microglia during homeostasis and disease.
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Microglía , Transcriptoma , Animales , Humanos , Ratones , Porcinos , Microglía/metabolismo , Roedores/genética , Análisis de Secuencia de ARN , Macrófagos/metabolismoRESUMEN
PURPOSE: Transsphenoidal surgery is an established treatment for pituitary adenomas. We examined outcomes and time points following transsphenoidal surgery for pituitary adenoma to identify reporting heterogeneity within the literature. METHODS: A systematic review of studies that reported outcomes for transsphenoidal surgery for pituitary adenoma 1990-2021 were examined. The protocol was registered a priori and adhered to the PRISMA statement. Studies in English with > 10 patients (prospective) or > 500 patients (retrospective) were included. RESULTS: 178 studies comprising 427,659 patients were included. 91 studies reported 2 or more adenoma pathologies within the same study; 53 studies reported a single pathology. The most common adenomas reported were growth hormone-secreting (n = 106), non-functioning (n = 101), and ACTH-secreting (n = 95); 27 studies did not state a pathology. Surgical complications were the most reported outcome (n = 116, 65%). Other domains included endocrine (n = 104, 58%), extent of resection (n = 81, 46%), ophthalmic (n = 66, 37%), recurrence (n = 49, 28%), quality of life (n = 25, 19%); and nasal (n = 18, 10%). Defined follow up time points were most reported for endocrine (n = 56, 31%), extent of resection (n = 39, 22%), and recurrence (n = 28, 17%). There was heterogeneity in the follow up reported for all outcomes at different time points: discharge (n = 9), < 30 days (n = 23), < 6 months (n = 64), < 1 year (n = 23), and > 1 year (n = 69). CONCLUSION: Outcomes and follow up reported for transsphenoidal surgical resection of pituitary adenoma are heterogenous over the last 30 years. This study highlights the necessity to develop a robust, consensus-based, minimum, core outcome set. The next step is to develop a Delphi survey of essential outcomes, followed by a consensus meeting of interdisciplinary experts. Patient representatives should also be included. An agreed core outcome set will enable homogeneous reporting and meaningful research synthesis, ultimately improving patient care.
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Adenoma , Neoplasias Hipofisarias , Humanos , Neoplasias Hipofisarias/cirugía , Neoplasias Hipofisarias/patología , Estudios Retrospectivos , Estudios Prospectivos , Calidad de Vida , Resultado del Tratamiento , Adenoma/cirugía , Adenoma/patología , Medición de Resultados Informados por el PacienteRESUMEN
PURPOSE: Heterogeneous reporting in baseline variables in patients undergoing transsphenoidal resection of pituitary adenoma precludes meaningful meta-analysis. We therefore examined trends in reported baseline variables, and degree of heterogeneity of reported variables in 30 years of literature. METHODS: A systematic review of PubMed and Embase was conducted on studies that reported outcomes for transsphenoidal surgery for pituitary adenoma 1990-2021. The protocol was registered a priori and adhered to the PRISMA statement. Full-text studies in English with > 10 patients (prospective), > 500 patients (retrospective), or randomised trials were included. RESULTS: 178 studies were included, comprising 427,659 patients: 52 retrospective (29%); 118 prospective (66%); 9 randomised controlled trials (5%). The majority of studies were published in the last 10 years (71%) and originated from North America (38%). Most studies described patient demographics, such as age (165 studies, 93%) and sex (164 studies, 92%). Ethnicity (24%) and co-morbidities (25%) were less frequently reported. Clinical baseline variables included endocrine (60%), ophthalmic (34%), nasal (7%), and cognitive (5%). Preoperative radiological variables were described in 132 studies (74%). MRI alone was the most utilised imaging modality (67%). Further specific radiological baseline variables included: tumour diameter (52 studies, 39%); tumour volume (28 studies, 21%); cavernous sinus invasion (53 studies, 40%); Wilson Hardy grade (25 studies, 19%); Knosp grade (36 studies, 27%). CONCLUSIONS: There is heterogeneity in the reporting of baseline variables in patients undergoing transsphenoidal surgery for pituitary adenoma. This review supports the need to develop a common data element to facilitate meaningful comparative research, trial design, and reduce research inefficiency.
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Adenoma , Neoplasias Hipofisarias , Humanos , Adenoma/cirugía , Adenoma/patología , Neoplasias Hipofisarias/cirugía , Neoplasias Hipofisarias/patología , Estudios Prospectivos , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
BACKGROUND: Infectious diseases of farmed and wild animals pose a recurrent threat to food security and human health. The macrophage, a key component of the innate immune system, is the first line of defence against many infectious agents and plays a major role in shaping the adaptive immune response. However, this phagocyte is a target and host for many pathogens. Understanding the molecular basis of interactions between macrophages and pathogens is therefore crucial for the development of effective strategies to combat important infectious diseases. RESULTS: We explored how porcine pluripotent stem cells (PSCs) can provide a limitless in vitro supply of genetically and experimentally tractable macrophages. Porcine PSC-derived macrophages (PSCdMs) exhibited molecular and functional characteristics of ex vivo primary macrophages and were productively infected by pig pathogens, including porcine reproductive and respiratory syndrome virus (PRRSV) and African swine fever virus (ASFV), two of the most economically important and devastating viruses in pig farming. Moreover, porcine PSCdMs were readily amenable to genetic modification by CRISPR/Cas9 gene editing applied either in parental stem cells or directly in the macrophages by lentiviral vector transduction. CONCLUSIONS: We show that porcine PSCdMs exhibit key macrophage characteristics, including infection by a range of commercially relevant pig pathogens. In addition, genetic engineering of PSCs and PSCdMs affords new opportunities for functional analysis of macrophage biology in an important livestock species. PSCs and differentiated derivatives should therefore represent a useful and ethical experimental platform to investigate the genetic and molecular basis of host-pathogen interactions in pigs, and also have wider applications in livestock.
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Virus de la Fiebre Porcina Africana , Enfermedades Transmisibles , Virus de la Fiebre Porcina Africana/genética , Animales , Interacciones Huésped-Patógeno/genética , Macrófagos , Células Madre , PorcinosRESUMEN
BACKGROUND: Type II odontoid fractures are known to have low fusion rates following conservative management with a hard collar. However, most patients are elderly with comorbidities and are not fit for surgery. The present study identified the rates of bony fusion, complications, and clinical outcomes following conservative management of type II odontoid fractures. METHODS: We included consecutive patients referred with a suspected odontoid fracture to a Major Trauma Centre in the UK between March 2015 and December 2017. Data including patient demographics, fracture management, complications and outcomes. Bony fusion was assessed by two neurosurgeons and one neuroradiologists. Results were analysed with simple statistics and chi-squared test. RESULTS: 102 patients were included in the study (mean age = 80.4 ± 15.3). 10 (9.8%) were managed surgically and 92 (90.2%) were managed conservatively with a hard collar, for a mean of 87 days. Patients were followed up for a mean of 28.1 months (range 1-855 days) until discharge. 37% developed collar complications, namely pain, stiffness and non-tolerance. Bony union was achieved in 37.3% of patients treated with a hard collar (versus 80% in the surgical group, p = 0.0096). Increasing age was an independent risk factor for non-union (p < 0.001). Of the patients without bony union, none reported symptoms, and 90% were discharged without a collar. CONCLUSION: The management of type II odontoid fractures are difficult in an elderly, co-morbid population. With conservative management fusion rates are low, and collar complications are not insignificant. However, outcomes are good regardless of union.
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Fracturas Óseas , Apófisis Odontoides , Fracturas de la Columna Vertebral , Anciano , Anciano de 80 o más Años , Humanos , Apófisis Odontoides/lesiones , Apófisis Odontoides/cirugía , Estudios Retrospectivos , Fracturas de la Columna Vertebral/cirugía , Resultado del TratamientoRESUMEN
The relationship between adolescents' communication with their significant others (mother, father, and best friend) and quality of life (KIDSCREEN) was investigated in 2262 Scottish adolescent pet owners. The variable attachment to pets was also tested and assessed as a mediator of this relationship. A positive relationship between adolescents' communication with their significant other (mother, father, and best friend) and quality of life decreased when controlling for attachment to dogs. In cat owners, a positive relationship between communication with a best friend and quality of life decreased when controlling for attachment to cats. In cat and dog owners, attachment to these pets predicted higher levels of quality of life. Higher attachment to dogs and cats was explained by good best friend (IV) and attachment to pets (DV) and best friends. Mediation effects of attachment to dogs and cats might be explained in terms of the caring activities associated with these types of pets.
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Comunicación , Amigos/psicología , Apego a Objetos , Relaciones Padres-Hijo , Mascotas/psicología , Calidad de Vida , Adolescente , Animales , Gatos , Perros , Femenino , Humanos , Masculino , Padres/psicología , EscociaRESUMEN
The aim of this study was to analyse spontaneous play behaviour in litters of domestic pigs (Sus scrofa) for sources of variation at individual and litter levels and to relate variation in play to measures of pre and postnatal development. Seven litters of commercially bred piglets (n = 70) were born (farrowed) within a penning system (PigSAFE) that provided opportunities for the performance of spontaneous play behaviours. Individual behaviour was scored based on an established play ethogram for 2 days per week over the 3 week study period. We found strong evidence of litter differences in play behaviour (F(6,63) = 27.30, p < 0.001). Of the variance in total play, 50% was attributable to differences between litters with a lesser proportion (11%) to between piglets within litters. We found similar evidence of litter differences when we analysed the separate play categories (e.g. for locomotor play: F(6,63) = 27.50, p < 0.001). For social and locomotor play the variance was partitioned in a broadly similar way to total play; however for object play the variance was distributed with a more even balance across and within litters. In terms of explanatory factors we found little evidence that at the litter level differences in play were associated with differences in general activity. Of the prenatal factors measured, we found that birth weight was positively associated with total play and the play categories (e.g. with total play: F(1,64) = 12.8, p < 0.001). We also found that postnatal piglet growth up to weaning (as a percentage of birth weight) had a significant positive association with total play and the play categories (e.g. with object play: F(1,66) = 20.55, p < 0.001). As found in other studies, on average males engaged in more social play (e.g. non-injurious play fighting: F(1,63) = 39.8, p < 0.001). Males also initiated more play bouts on average than females (F(1,62) = 4.41, p = 0.040). We conclude that the study of differences between litters and individuals provides a robust approach to understanding factors potentially influencing play behaviour in the pig. This work also provides support for the use of play as a welfare indicator in pre-weaned piglets as the litter differences in play we observed were associated positively with physical development.
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The importance of maternal care in commercial pig production is largely ignored. The sow has little possibility to interact with her piglets, and piglets are often subjected to early weaning or artificial rearing. This study aimed to investigate aspects of physiological and behavioural maternal provisioning that contribute to offspring outcomes. We hypothesised that better maternal care and nutritional provisioning would relate positively to piglet immunity, growth and behaviour. Nineteen sows and their litters were studied in free-farrowing pens. Oxytocin and tumour necrosis factor-α in colostrum/milk and salivary cortisol were sampled from sows throughout lactation. Sows were assessed for dominance rank, response to handling, maternal defensiveness, suckling initiation and termination, posture and sow-piglet contact. Piglets were weighed, measured for body mass index (BMI) and sampled for blood (Immunoglobulin G; at birth). After weaning, they experienced a human approach test (HAT) and novel object test. Correlations were explored between individual sow characteristics, individual piglet outcomes, and between sow characteristics and piglet outcomes averaged by litter. Significant correlations between sow and piglet factors were analysed at the litter level in mixed models with piglet outcomes as response variables and sow characteristics as predictor variables, while accounting for sow parity, litter size and batch. Litters grew faster when their sow had lower cortisol values (P = 0.03), while sows with lower cortisol levels had more successful suckling bouts and engaged in greater amounts of sow-piglet contact. Litters had a lower BMI at weaning when the sow had a higher milk fat percentage at d3. Litters of the most dominant sows took longer to approach the human in the HAT, while litters of sows with higher cortisol at d0 took longer to approach the novel object when assessed on correlations (r = 0.82, P < 0.001) but not when the model accounted for parity and litter size (P = 0.35). Only some of the measured nutritive and non-nutritive sow factors influenced litter performance and behaviour, with parity and litter size also playing a role. Given the continued increase in litter size, but also the interest in loose-housed lactation pens for sows, further research on sows' maternal investment and how it can be optimised is warranted.
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Hidrocortisona , Parto , Embarazo , Femenino , Humanos , Animales , Porcinos , Animales Recién Nacidos , Lactancia/fisiología , Destete , Tamaño de la CamadaRESUMEN
Scientific assessment of affective states in animals is challenging but vital for animal welfare studies. One possible approach is Qualitative Behavioural Assessment (QBA), a 'whole animal' methodology which integrates information from multiple behavioural signals and styles of behavioural expression (body language) directly in terms of an animal's emotional expression. If QBA provides a valid measure of animals' emotional state it should distinguish between groups where emotional states have been manipulated. To test this hypothesis, QBA was applied to video-recordings of pigs, following treatment with either saline or the neuroleptic drug Azaperone, in either an open field or elevated plus-maze test. QBA analysis of these recordings was provided by 12 observers, blind to treatment, using a Free Choice Profiling (FCP) methodology. Generalised Procrustes Analysis was used to calculate a consensus profile, consisting of the main dimensions of expression. Dimension one was positively associated with terms such as 'Confident' and 'Curious' and negatively with 'Unsure' and 'Nervous'. Dimension two ranged from 'Agitated'/'Angry' to 'Calm'/'Relaxed'. In both tests, Azaperone pre-treatment was associated with a more positive emotionality (higher scores on dimension one reflecting a more confident/curious behavioural demeanour) than control pigs. No effect of drug treatment on dimension two was found. Relationships between qualitative descriptions of behaviour and quantitative behavioural measures, taken from the same recordings, were found. Overall, this work supports the use of QBA for the assessment of emotionality in animals.
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'Tickling' induces positive affective states in laboratory rats as evidenced by the production of 50-kHz ultrasonic vocalisations (USVs), although this has mostly been investigated in males. Juvenile rats emit distinctive 50-kHz USV subtypes. Frequency-modulated (FM) 50-kHz USVs are thought to be associated with positive affect and flat 50-kHz USVs with social communication. FM and flat USVs are produced by both sexes during tickling, but it is unclear whether these calls are produced in relation to particular play-related behaviours, and whether USV subtypes are used in a sexually dimorphic manner during tickling. We tested the hypotheses that FM USVs are associated with tickle-induced play behaviours in a sex-specific way, and that flat USVs are associated with non-play activities. Rats were allocated to one of two treatment groups: tickling (tickled, n = 16/sex) or no hand contact (control, n = 16/sex). Play behaviours (hopping, darting and hand approaches) and FM and flat USVs emitted during the testing session were quantified for each rat, with the frequency of FM and flat USVs made in anticipation of, and during, each behaviour analysed. In females, play behaviours were associated with more flat USVs than in males (before and during; p < 0.001), irrespective of treatment. FM USVs were paired with hopping and darting (before and during; p < 0.001), and in anticipation of hand approaches (p < 0.001) in both tickled females and males compared to controls (both sexes) suggesting that FM USVs are linked with play behaviour. The higher call rate of flat USVs paired with play behaviour in females suggests that there may be sex differences in the role of flat USVs during play. This result is evidence of sex differences in tickle-induced behaviours and has implications for our understanding of the function of different USVs in juvenile female and male rats.
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Caracteres Sexuales , Vocalización Animal , Animales , Femenino , Masculino , Ratas , UltrasonidoRESUMEN
Rat tickling is a heterospecific interaction for experimenters to mimic the interactions of rat play, where they produce 50 kHz ultrasonic vocalisations (USV), symptoms of positive affect; tickling can improve laboratory rat welfare. The standard rat tickling protocol involves gently pinning the rat in a supine position. However, individual response to this protocol varies. This suggests there is a risk that some rats may perceive tickling as only a neutral experience, while others as a positive one, depending on how tickling is performed. Based on our research experiences of the standard tickling protocol we have developed a playful handling (PH) protocol, with reduced emphasis on pinning, intended to mimic more closely the dynamic nature of play. We will test whether our PH protocol gives rise to more uniform increases in positive affect across individuals relative to protocols involving pinning. We will compare the response of juvenile male and female Wistar rats as: Control (hand remains still against the side of the test arena), P0 (PH with no pinning), P1 (PH with one pin), P4 (PH with four pins). P1 and P4 consist of a background of PH, with treatments involving administration of an increasing dosage of pinning per PH session. We hypothesise that rats exposed to handling protocols that maximise playful interactions (where pinning number per session decreases) will show an overall increase in total 50 kHz USV as an indicator of positive affect, with less variability. We will explore whether behavioural and physiological changes associated with alterations in PH experience are less variable. We propose that maximising the numbers of rats experiencing tickling as a positive experience will reduce the variation in response variables affected by tickling and increase the repeatability of research where tickling is applied either as a social enrichment or as a treatment.
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Bienestar del Animal , Interacción Humano-Animal , Ratas Wistar , Animales , Femenino , Masculino , Ratas , Ultrasonido , Vocalización AnimalRESUMEN
This study assessed the effect of pre-natal social stress and post-natal pain on the reproductive development of young (approximately day 40) pigs. Male pigs carried by sows that were stressed by mixing with unfamiliar older sows for two 1-week periods during mid-pregnancy had lower plasma testosterone (0.54 vs 0.86 ng/ml, S.E.D.=0.11; P=0.014) and oestradiol (E(2); 22.9 vs 38.7 pg/ml, S.E.D.=7.80; P=0.021) concentrations compared with males carried by unstressed control sows. Although there was no effect of pre-natal stress on female E(2) concentrations, female pigs carried by stressed sows had fewer primordial ovarian follicles (log -4.32/µm(2) vs -4.00/µm(2), s.e.d.=0.136; P=0.027). Tail amputation on day 3 after birth reduced E(2) concentrations in female (4.78 vs 6.84 pg/ml, s.e.d.=0.86; P=0.03) and in male (25.6 vs 34.9 pg/ml, S.E.D.=3.56; P=0.021) pigs and reduced both testis weight (0.09% of body weight vs 0.10% of body weight, S.E.D.=0.003; P=0.01) and the percentage of proliferating Leydig cells (1.97 vs 2.12, S.E.D.=0.114; P=0.036) compared with sham-amputated littermate controls. There was a significant (P=0.036) interaction between the effects of pre-natal stress and post-natal pain on testicular expression of the steroidogenic enzyme 17α-hydroxylase, such that amputation increased expression in pigs born to control sows, but reduced expression in animals born to stressed sows. This study shows that stressful procedures associated with routine animal husbandry can disrupt the developing reproductive axis.
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Crianza de Animales Domésticos , Dolor/fisiopatología , Efectos Tardíos de la Exposición Prenatal , Desarrollo Sexual , Estrés Psicológico , Animales , Femenino , Masculino , Embarazo , Caracteres Sexuales , Estrés Fisiológico , PorcinosRESUMEN
The importance given to minimising health issues and promoting natural behaviours is a polarising issue within farm animal welfare. It is predominantly thought that members of the public prioritise animals being able to behave naturally over other aspects of farm animal welfare, such as addressing health issues. However, public perspectives may be more multi-dimensional than is generally thought, with the importance given to these different elements of welfare dependent on the situation and state of the animals in question. To examine this, a factorial survey using vignettes, which experimentally manipulated the different levels of health (high health vs. low health) and natural behaviour provision (high behaviour vs. low behaviour), was completed by a sample (n = 810) representative of the UK population (on age, gender, ethnicity). Contrary to the predominant view, this study found animal health had the greatest effect on participants' judgements, explaining more of the variance in their assessments of animal welfare than any other factor. However, findings also indicated that participants considered animal welfare to be most positive when both health issues are minimised and natural behaviours are promoted. Attitudes to natural behaviours also varied more between participants, with females, individuals who do not (regularly) eat meat and those with a greater belief in animal mind giving greater priority to natural behaviours. In the context of public and private welfare standards seeking to meet public expectations, this study provides important insights into how public perspectives of animal welfare are more nuanced than previously thought, influenced by the context of the animal, the aspect of welfare in question and personal characteristics.
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Bienestar del Animal , Animales Domésticos , Conducta Animal , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Animales , Actitud , Femenino , Humanos , Masculino , Persona de Mediana Edad , Reino Unido , Adulto JovenRESUMEN
This review paper provides an integrative account regarding neurophysiological correlates of positive emotions and affect that cumulatively contribute to the scaffolding for happiness and wellbeing in humans and other animals. This paper reviews the associations among neurotransmitters, hormones, brain networks, and cognitive functions in the context of positive emotions and affect. Consideration of lifespan developmental perspectives are incorporated, and we also examine the impact of healthy social relationships and environmental contexts on the modulation of positive emotions and affect. The neurophysiological processes that implement positive emotions are dynamic and modifiable, and meditative practices as well as flow states that change patterns of brain function and ultimately support wellbeing are also discussed. This review is part of "The Human Affectome Project" (http://neuroqualia.org/background.php), and in order to advance a primary aim of the Human Affectome Project, we also reviewed relevant linguistic dimensions and terminology that characterizes positive emotions and wellbeing. These linguistic dimensions are discussed within the context of the neuroscience literature with the overarching goal of generating novel recommendations for advancing neuroscience research on positive emotions and wellbeing.
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Felicidad , Neurociencias , Animales , Encéfalo , Emociones , Humanos , LingüísticaRESUMEN
Play is a strong outwardly directed, emotional behaviour and can contagiously spread between individuals. It has been suggested that high-playing animals could 'seed' play in others, spreading positive affective states. Despite the current interest in play contagion there has been no previous attempt to measure the strength of the play contagion effect. The calf (Bos taurus) is ideal for testing the strength of play contagion as play in calves is strongly related to energy intake from milk. We manipulated play in calves through their milk allowances and housed the calves in uniform groups all on the same milk allowance (high = UHigh or low = ULow) or in mixed groups with calves in the same group receiving either a high (= MHigh) or low (= MLow) milk allowance. We measured locomotor play using accelerometers on two consecutive days when calves were four and eight weeks old, in order to study play contagion over a protracted developmental window. We anticipated that differences in the level of play contagion between treatment groups would result in difference in the play levels observed in the MLow and ULow individuals. Contrary to our expectations we found that spontaneous play was suppressed in the high-milk calves housed in mixed groups (MHigh), in comparison to calves housed with group mates all receiving high-milk (UHigh). These results are the first to quantify a negative play contagion effect, particularly in a situation of long-term contact, and may suggest that negative contagion has a stronger effect on play behaviour than positive contagion.
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Animales Recién Nacidos/fisiología , Animales Recién Nacidos/psicología , Conducta Animal/fisiología , Bovinos/fisiología , Bovinos/psicología , Dieta/veterinaria , Ingestión de Energía/fisiología , Leche , Juego e Implementos de Juego/psicología , Animales , Femenino , Vivienda para Animales , Humanos , MasculinoRESUMEN
Pre-natal stress (PNS) or undernutrition can have numerous effects on an individual's biology throughout their lifetime. Some of these effects may be adaptive by allowing individuals to tailor their phenotype to environmental conditions. Here we investigated, in the domestic pig Sus scrofa, whether one possible consequence of a predicted adverse environment could be altered pain perception. The behavioural response of piglets to the surgical amputation ('docking') of their tail or a sham procedure was measured for 1 min in piglets born to mothers who either experienced mid-gestation social stress or were left undisturbed throughout pregnancy. A behavioural pain score was found to predict the docked status of piglets with high discriminant accuracy. Piglets exposed to PNS had a significantly higher pain score than controls, and for each litter of tail-docked piglets, the average pain score was correlated with mid-gestation maternal cortisol levels. The data presented here provide evidence that the experience of stress in utero can result in a heightened acute response to injury in early life. Speculatively, this may represent an adaptive alteration occurring as a consequence of a pre-natal 'early warning' of environmental adversity.
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Animales Recién Nacidos/fisiología , Conducta Animal , Preñez , Adaptación Psicológica , Animales , Oído Externo/lesiones , Femenino , Hidrocortisona/metabolismo , Dolor , Umbral del Dolor , Embarazo , PorcinosRESUMEN
This study aimed to compare the prevalence of lameness on organic and non-organic dairy farms in the United Kingdom (UK) and to assess which cow and farm factors influenced lameness levels. Forty organic and 40 non-organic dairy farms across the UK were repeatedly visited over a 2.5 year period. On each visit all milking cows were locomotion scored, and information about farm housing, management and husbandry practices was recorded on-farm. Over the whole study, the mean herd lameness prevalence was 16.2%, 16.3% and 19.3% in the autumn, winter and spring observation periods, respectively. Lameness prevalence was lower (P=0.012) on organic farms compared to non-organic farms. Numerous specific factors were found to significantly influence the prevalence of lameness. This study provided evidence that organic management reduced herd lameness. It supported previous research which suggested that lameness is a serious problem on many farms in the UK and further emphasised the multi-factorial aetiology of lameness problems.
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Crianza de Animales Domésticos/métodos , Bienestar del Animal , Enfermedades de los Bovinos/epidemiología , Industria Lechera/métodos , Cojera Animal/epidemiología , Crianza de Animales Domésticos/normas , Animales , Bovinos , Enfermedades de los Bovinos/etiología , Enfermedades de los Bovinos/prevención & control , Industria Lechera/normas , Femenino , Vivienda para Animales/normas , Cojera Animal/etiología , Cojera Animal/prevención & control , Locomoción , Factores de Riesgo , Estaciones del Año , Reino Unido/epidemiologíaRESUMEN
To support the furtherance of positive animal welfare, there is a need to develop meaningful and practical positive welfare indicators for on-farm welfare assessment. Considering the perspectives of farmers is arguably critical in this regard. Doing so helps ensure positive welfare indicators reflect farmers' existing welfare norms and attitudes and, are thus, of practical relevance to them. However, a key issue for such development is the dearth of knowledge on farmers' perspectives of positive welfare. To address this, this study uses qualitative interviews to directly examine livestock farmers' perspectives of positive welfare. Findings reveal that farmers describe elements of positive welfare which are broadly in line with indicators suggested in the positive welfare literature. These elements include animal autonomy, play, positive affect, positive human-animal relationships, social interaction, and appropriate genetic selection. Additionally, this study finds that farmers construct the reduction of negative aspects of welfare as their primary management concern and mostly construct positive welfare as arising indirectly from this. Insights into the importance that farmers of different sectors and systems give to different aspects of positive welfare indicators are also explored. The implications of these findings and the similitudes between farmers' perspectives and the positive welfare literature are discussed.
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It is claimed that positive animal welfare (PAW) developed over the last decade in reaction to animal welfare focusing too much on avoiding negatives. However, it remains unclear what PAW adds to the animal welfare literature and to what extent its ideas are new. Through a critical review of the PAW literature, we aim to separate different aspects of PAW and situate it in relation to the traditional animal welfare literature. We find that the core PAW literature is small (n = 10 papers) but links to wider areas of current research interest. The PAW literature is defined by four features: (1) positive emotions which is arguably the most widely acknowledged; (2) positive affective engagement which serves to functionally link positive emotions to goal-directed behavior; (3) quality of life which serves to situate PAW within the context of finding the right balance of positives over negatives; (4) happiness which brings a full life perspective to PAW. While the two first points are already part of welfare research going back decades, the two latter points could be linked to more recent research agendas concerning aggregation and how specific events may affect the ability of animals to make the best of their lives.