RESUMEN
This paper presents the analytical solution of radial vibration of a rolling cylinder submitted to a time-varying point force. In the simplest situation of simply supported edges and zero in-plane vibration, the cylinder is equivalent to an orthotropic pre-stressed plate resting on a visco-elastic foundation. We give the closed-form solution of vibration as a series of normal modes whose coefficients are explicitly calculated. Cases of both deterministic and random forces are examined. We analyse the effect of rolling speed on merging of vibrational energy induced by Doppler's effect for the example of rolling tyre.
RESUMEN
INTRODUCTION: We report a case of occupational hypersensitivity pneumonitis in a patient handling chicory leaves. CASE REPORT: The diagnosis was based symptoms of broncho-alveolitis with pyrexia, positive precipitins to moulds present on chicory, especially Fusarium, and the disappearance of the clinical and radiological manifestations following cessation of exposure to chicory. CONCLUSION: "Chicory worker's lung" is an occupational disease which should be considered in cases of respiratory symptoms suggestive of hypersensitivity pneumonitis and chronic exposure to chicory leaves.
Asunto(s)
Alveolitis Alérgica Extrínseca/etiología , Cichorium intybus/efectos adversos , Enfermedades Profesionales/etiología , Adulto , Femenino , Humanos , Hojas de la Planta/efectos adversosRESUMEN
INTRODUCTION: Catamenial haemoptysis is a rare clinical entity resulting from the presence of ectopic intra pulmonary endometrial tissue, either parenchymatous or endobronchial. The main diagnostic criterion is the periodic character of the haemoptysis which is synchronous with menstruation. CASE REPORT: The authors report a case of catamenial haemoptysis due to endobronchial endometriosis in a 46 year old menopausal woman receiving hormone replacement treatment (HRT). She presented with 3 episodes of haemoptysis synchronous with the first days of her menstrual cycle. A thoracic CT scan showed ground glass lesions with micronodulation. Bronchoscopy showed violacious lesions bleeding on contact. The endobronchial and CT abnormalities had disappeared by day 5. After withdrawal of the HRT the haemoptysis did not recur during a follow-up of 2 years. CONCLUSION: Endobronchial endometrioisis remains a rare occurrence. This is the first case reported in a menopausal woman with artificial cycles receiving hormone replacement therapy.
Asunto(s)
Terapia de Reemplazo de Estrógeno/efectos adversos , Hemoptisis/inducido químicamente , Endometriosis/inducido químicamente , Femenino , Humanos , Enfermedades Pulmonares/inducido químicamente , Persona de Mediana EdadRESUMEN
AIM: To appraise the tolerance and efficacy of an induction of tolerance protocol to infliximab permitting the re-administration of the drug to patients with Crohn's disease having had infusion reactions requiring suspension of treatment. METHODS: Fourteen patients were included in the induction of tolerance protocol. Each infusion of infliximab (5 mg/kg) was divided into 11 escalating 15 min increments over a 3-h time period. The induction of tolerance procedure was repeated for subsequent infusions. RESULTS: Ten patients (71.4%) received all the three infusions for the induction treatment. Nine (64.3%) had a significant response and six (48.8%) still benefited from infliximab infusions. Seven patients (50%) achieved a complete remission, after a mean of 2.5 (two to three) infusions. Four patients (28.6%) had no response and the protocol was stopped. Three patients (21.4%) experienced mild immediate hypersensitivity reactions, which were controlled, two patients (14.2%) experienced severe immediate hypersensitivity reactions, leading to interruption of the treatment and one patient developed a delayed hypersensitivity reaction. CONCLUSION: Our induction of tolerance protocol allows some patients who have experienced severe or repetitive infusion reactions to infliximab to be safely retreated with the drug in a hospitalized setting, with a clinical response achieved in a majority of these patients.
Asunto(s)
Antiinflamatorios/efectos adversos , Anticuerpos Monoclonales/efectos adversos , Enfermedad de Crohn/tratamiento farmacológico , Adulto , Antiinflamatorios/administración & dosificación , Anticuerpos Monoclonales/administración & dosificación , Protocolos Clínicos , Esquema de Medicación , Hipersensibilidad a las Drogas/etiología , Femenino , Fármacos Gastrointestinales/administración & dosificación , Fármacos Gastrointestinales/efectos adversos , Humanos , Hipersensibilidad Inmediata/inducido químicamente , Infliximab , Infusiones Intravenosas/efectos adversos , Masculino , Persona de Mediana Edad , Resultado del TratamientoRESUMEN
Polymyositis is characterised by an inflammatory reaction in skeletal muscle with a variable degree of muscular weakness and associated with skin lesions in the case of dermatomyositis. Involvement of the muscles of deglutition and the diaphragm may lead to inhalation pneumonia and acute or chronic respiratory failure, often hypercapnic. The other respiratory manifestations are diffuse interstitial pneumonitis (DIP), usually non-specific, and very occasionally pulmonary arterial hypertension. The development of DIP during polymyositis is a grave prognostic factor, respiratory involvement being one of the main causes of morbidity and mortality. The onset of DIP is acute in between 30 and 47% of cases. Anti-synthetase antibodies (particularly anti-JO-1) are positive in about 75% of cases. Treatment is usually with a combination of immunosuppressants and corticosteroids without any immunosuppressants therapy having shown a superiority.
Asunto(s)
Enfermedades Pulmonares/etiología , Polimiositis/complicaciones , Corticoesteroides/uso terapéutico , Anticuerpos Antinucleares/inmunología , Dermatomiositis/complicaciones , Diagnóstico Diferencial , Quimioterapia Combinada , Humanos , Inmunosupresores/uso terapéutico , Enfermedades Pulmonares/diagnóstico , Enfermedades Pulmonares/tratamiento farmacológico , Enfermedades Pulmonares/inmunología , Enfermedades Pulmonares Intersticiales/etiología , Polimiositis/diagnóstico , Polimiositis/tratamiento farmacológico , Polimiositis/inmunología , Pronóstico , Resultado del TratamientoRESUMEN
The p53 protein is known to trans-activate a number of genes by specific binding to a consensus sequence containing two decamers of the type: PuPuPuCA/TT/AGPyPyPy. In order to identify new p53 trans-activated genes, we defined a set of criteria for computer search of p53-responsive elements. Based on experimental data, we proposed an extended consensus sequence composed of the two decamers of the El-Deiry consensus sequence flanked by two additional ones. A maximum of 3 bp substitutions was accepted for the two decamers of the El-Deiry consensus sequence, as well as for each additional decamer, except when the two decamers of the El-Deiry consensus sequence are contiguous. In this case, each additional decamer is allowed to bear one base insertion or deletion between the median C and G. This set of criteria was validated by identifying within the promoter region of the IGF-BP3 gene the existence of a novel p53-responsive element whose functional significance was verified. By limiting our computer search to Vertebrate genes involved in cell cycle regulation, cellular adhesion or metastatic processes and to gene families most often found in HOVERGEN database, 7785 gene sequences were first analysed. Among the oncogenes, kinases, proteases and structural proteins, 55 new genes were selected; six of them were retrieved in more than one species.
Asunto(s)
ADP-Ribosil Ciclasa , Antígenos CD , Regiones Promotoras Genéticas/genética , Análisis de Secuencia de ADN , Activación Transcripcional/genética , Proteína p53 Supresora de Tumor/genética , Secuencia de Bases , Cloranfenicol O-Acetiltransferasa/genética , Cloranfenicol O-Acetiltransferasa/metabolismo , Proteínas Ligadas a GPI , Genes Reporteros , Células HeLa , Humanos , Glicoproteínas de Membrana/metabolismo , Datos de Secuencia Molecular , Somatomedinas/metabolismo , Proteína p53 Supresora de Tumor/fisiologíaRESUMEN
INTRODUCTION: Corticosteroids are widely used for the management of many diseases because of anti-inflammatory and immunomodulatory properties. Sulfite intolerance is well known but immediate hypersensitivity to corticosteroids is rarer and must be reported. EXEGESIS: We report a case of a 38-year-old man, followed since 1986 for asthma with sulfite intolerance. He twice developed urticaria after ingesting betalactam antibiotics associated with corticosteroids of the group A, according to the Matura and Goossens's classification. The allergy inquiry confirmed not only a beta-lactame allergy but also in corticosteroids of the group A. Intradermal reactions with methylprednisolone and hydrocortisone were positive at 7 mm for methylprednisolone on 1/1000 dilution and at 4 mm for hydrocortisone on 1/10 dilution. Thus immediate allergy to corticosteroids was confirmed. But those skin tests were negative for corticosteroids of group B and C. In order to propose an alternative therapy for acute asthma, an intravenous injection of 4 mg of betamethasone was performed without inducing immediate and delayed allergic reactions. CONCLUSION: Corticosteroid allergy is rare and can be fatal. Many asthmatic or acetylsalicylic acid intolerant patients may be concerned. The diagnostic of corticosteroid allergy have to be confirmed by clinical history and skin tests of the different groups to try to identify one or more allergens and to propose a alternative treatment if necessary.
Asunto(s)
Corticoesteroides/efectos adversos , Hipersensibilidad a las Drogas/etiología , Administración Oral , Corticoesteroides/administración & dosificación , Adulto , Humanos , Masculino , Factores de TiempoRESUMEN
BACKGROUND: Infliximab is a chimeric monoclonal antibody directed against tumour necrosis factor-alpha that has been shown to improve chronic refractory and fistulating Crohn's disease. Infliximab infusions have been associated both with immediate and delayed reactions. MATERIAL AND METHODS: Desensitisation was performed in four patients who had experienced immediate reactions to infliximab infusions and in one who had developed a delayed reaction. No therapeutic alternatives were available for these patients. Before desensitisation, skin tests were performed. RESULTS: Skin-tests were negative for all patients. Desensitisation was performed with serial dilutions of infliximab with monitoring of vital signs before each increment. After parenteral desensitisation, all five patients were able to tolerate infliximab infusion without complications or any requirement for antihistamines or steroids. However, two patients who had initially presented with an immediate reaction to infliximab experienced arthralgia and myalgia similar to a "serum sickness-type" of reaction 6 to 10 days after desensitisation. CONCLUSION: Even if there is no evidence of an allergic mechanism in infusion reactions to infliximab, successful desensitization can be achieved for patients experiencing acute reactions. The mechanism of desensitisation remains presently unknown. It is not yet possible to say if desensitization will be effective in preventing delayed reactions.
Asunto(s)
Anticuerpos Monoclonales/efectos adversos , Enfermedad de Crohn/tratamiento farmacológico , Desensibilización Inmunológica , Hipersensibilidad a las Drogas/etiología , Hipersensibilidad a las Drogas/terapia , Adulto , Anticuerpos Monoclonales/uso terapéutico , Femenino , Humanos , Infliximab , Masculino , Persona de Mediana Edad , Factor de Necrosis Tumoral alfaRESUMEN
The accessibility of each 30S subunit protein to their cognate antibodies (IgG or Fabs) having been previously well established, the effect of their in situ specific neutralization by monovalent IgG fragments (FabI) are reported for five reactions: 1) T4 and R17 RNA directed protein synthesis: 2) polyphenylalanine synthesis: 3) enzymatic Phe-tRNA binding in the presence of 30S + 50W subunits: 4) fMet-tRNAf binding to the 30S subunit in the presence of initiation factors IF1, IF2, IF3; 5) coupling with lambda plac DNA transcription of the initial translation step (i.e., interaction of IF3 activated 30S subunits with nascent mRNA, in the absence of tRNA). According to evident similarities in their inhibition pattern concerning the five reactions tested, 30S subunit proteins can be classified in five categories which are discussed in terms of functional topography.
Asunto(s)
Proteínas Bacterianas/fisiología , Escherichia coli/metabolismo , Proteínas Ribosómicas/fisiología , Fragmentos Fab de Inmunoglobulinas , Pruebas de Neutralización , Poli U/metabolismo , Biosíntesis de Proteínas , ARN Bacteriano/metabolismo , ARN Mensajero/metabolismo , ARN de Transferencia/metabolismo , Proteínas Ribosómicas/clasificación , Transcripción GenéticaRESUMEN
Computed tomography imaging in a patient with a pericardial effusion showed fluid collection in the superior recessus of pericardium confirmed by mediastinoscopy. Topographic and morphologic criteria and anatomic bases of the radiologic diagnosis are described, and emphasis placed on the fact that because of density variations in pericardial effusions the latter has to be added to the already long list of abnormal opacities of Barety's space.
Asunto(s)
Derrame Pericárdico/diagnóstico por imagen , Pericardio/diagnóstico por imagen , Tomografía Computarizada por Rayos X , Anciano , Enfermedad Crónica , Femenino , Humanos , Derrame Pericárdico/patología , Pericardio/anatomía & histología , Pericardio/patologíaRESUMEN
A report is made of 3 observations of vascular constrictions in the knee hollow of three young adults: two constrictions of the popliteal artery, one by abnormal insertion of a tendon of the gastrocnemius muscle and of the arterial passage, the other by a fibrous band surrounding the artery. In one observation, the disorder was revealed by acute ischemia of one limb. The third observation was of a sural phlebitis with secondary repetition at the constriction of the popliteal vein by Soleaire's arcade. In all cases examination using the Doppler effect, in baseline and especially dynamic position, gives a diagnosis and allows postoperative monitoring. Bilateral arteriography, both static and dynamic, allows a precise diagnosis to be given and reveals the extent of vascular lesions, which conditions the operative technique. Treatment is always surgical, with exploration of the contralateral side if a constriction is suspected, even if it is asymptomatic.
Asunto(s)
Arteria Poplítea , Vena Poplítea , Adulto , Constricción Patológica/complicaciones , Humanos , Isquemia/etiología , Pierna/irrigación sanguínea , Masculino , Flebitis/etiologíaAsunto(s)
Antiasmáticos/uso terapéutico , Anticuerpos Monoclonales/uso terapéutico , Asma/tratamiento farmacológico , Terapia Biológica , Administración por Inhalación , Corticoesteroides/administración & dosificación , Corticoesteroides/uso terapéutico , Adulto , Animales , Antiasmáticos/administración & dosificación , Antiinflamatorios/administración & dosificación , Antiinflamatorios/uso terapéutico , Antiinflamatorios no Esteroideos/administración & dosificación , Antiinflamatorios no Esteroideos/uso terapéutico , Anticuerpos Antiidiotipos , Anticuerpos Monoclonales/administración & dosificación , Anticuerpos Monoclonales Humanizados , Asma/inmunología , Beclometasona/administración & dosificación , Beclometasona/uso terapéutico , Niño , Ensayos Clínicos Controlados como Asunto , Modelos Animales de Enfermedad , Eosinofilia/tratamiento farmacológico , Etanercept , Predicción , Glucocorticoides/administración & dosificación , Glucocorticoides/uso terapéutico , Haplorrinos , Humanos , Síndrome Hipereosinofílico/tratamiento farmacológico , Inmunoglobulina E/inmunología , Inmunoglobulina G/administración & dosificación , Inmunoglobulina G/uso terapéutico , Inmunosupresores/administración & dosificación , Inmunosupresores/uso terapéutico , Interleucina-5/inmunología , Omalizumab , Receptores del Factor de Necrosis Tumoral/administración & dosificación , Receptores del Factor de Necrosis Tumoral/uso terapéutico , Factores de Tiempo , Factor de Necrosis Tumoral alfa/antagonistas & inhibidoresAsunto(s)
Proteínas Bacterianas/aislamiento & purificación , Unión Proteica , ARN Mensajero/análisis , ARN de Transferencia/análisis , Ribosomas/análisis , Centrifugación Zonal , Cromatografía DEAE-Celulosa , Cromatografía por Intercambio Iónico , Colifagos , ADN Viral/análisis , Electroforesis Discontinua , Escherichia coli , Calor , Hidroxiapatitas , Metionina , Microscopía Electrónica , Fenilalanina , ARN Nucleotidiltransferasas , ARN Bacteriano/análisis , ARN Bacteriano/aislamiento & purificación , ARN Mensajero/aislamiento & purificación , ARN de Transferencia/aislamiento & purificación , Factores de TiempoAsunto(s)
Sitios de Unión , Biosíntesis de Péptidos , ARN de Transferencia , Ribosomas , Antibacterianos/farmacología , Isótopos de Carbono , Dimetilsulfóxido/farmacología , Código Genético , Hibridación Genética , Conformación de Ácido Nucleico , Extensión de la Cadena Peptídica de Translación , Iniciación de la Cadena Peptídica Traduccional , Puromicina/farmacología , Estreptomicina/farmacología , Tetraciclina/farmacología , TritioAsunto(s)
Proteínas de Unión al ADN/metabolismo , ADN/metabolismo , Proteínas Nucleares/metabolismo , Animales , Autorradiografía/métodos , Sitios de Unión , ADN/genética , ADN/aislamiento & purificación , ADN Viral/genética , ADN Viral/metabolismo , Proteínas de Unión al ADN/aislamiento & purificación , Electroforesis en Gel de Poliacrilamida/métodos , Genes , Immunoblotting/métodos , Indicadores y Reactivos , Virus del Tumor Mamario del Ratón/genética , Virus del Tumor Mamario del Ratón/metabolismo , Ratones , Proteínas Nucleares/aislamiento & purificación , Secuencias Reguladoras de Ácidos Nucleicos , Secuencias Repetitivas de Ácidos Nucleicos , Mapeo Restrictivo , Especificidad por Sustrato , TATA BoxRESUMEN
The synthesis and DNA binding activity of purified nuclear non-histone proteins from mouse erythroblasts and myoblasts have been compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, affinity chromatography, and protein blotting. The labeled non-histone proteins bound to mouse total DNA clearly differ between erythroid and muscle cell lines, but these differences mainly reflect the qualitative changes observed in their pattern of synthesis. By contrast, a cloned genomic mouse beta-globin DNA fragment binds specifically several proteins (100K, 65K, 50K, 45K, and 34K) from erythropoietic Friend cells and does not bind any protein in the corresponding fraction from myoblasts. The specificity of these DNA protein interactions requires a NaCl concentration of 0.1 M and a low protein/DNA ratio. In these conditions lambda DNA binds the above proteins to only a small extent. During the dimethyl sulfoxide induced terminal differentiation of Friend mouse erythroleukemia (MEL) cells, there is an apparent overall decrease of total as well as globin DNA binding to the nuclear non-histone proteins but not to the histones, whereas no significant qualitative changes are detected.
Asunto(s)
Proteínas Cromosómicas no Histona/genética , ADN/genética , Genes , Globinas/genética , Leucemia Experimental/metabolismo , Animales , Línea Celular , Proteínas Cromosómicas no Histona/aislamiento & purificación , Ratones , Unión ProteicaRESUMEN
Restriction fragments of the mouse beta major globin gene and of the long terminal repeat (LTR) DNA fragment of the mouse mammary tumor provirus as a control, were used to analyze the specificity of DNA-protein interactions in nuclear extracts of mouse erythroleukemia (MEL) cells and of other differentiated mouse cultured cell lines. After gel electrophoresis and transfer to nitrocellulose, DNA-binding proteins with a preferential affinity for the cloned beta-globin genomic sequence were characterized and related to the level of globin gene expression during induction of differentiating mouse erythroblasts. Two proteins (110 K and 75 K) appear in differentiated MEL cells while another one (100 K), for which we have localized the binding site on the beta-globin gene, is present only in immature MEL cells.
Asunto(s)
Eritroblastos/metabolismo , Genes , Globinas/genética , Leucemia Eritroblástica Aguda/metabolismo , Leucemia Experimental/metabolismo , Nucleoproteínas/metabolismo , Animales , Diferenciación Celular , Línea Celular , ADN/metabolismo , Eritroblastos/citología , Ratones , Unión ProteicaRESUMEN
The product from the in vitro transcription of native lambdaDNA by purified E. coli RNA polymerase does not stimulate total amino acid incorporation although it can program ribosomes for the first peptide bond synthesis in the presence of the three initiation factor A, B and C.