Three-dimensional tomographic reconstruction using Voronoi weighting.

Three-dimensional tomographic reconstruction requires careful selection of the illumination angles, often under certain measurement constraints. When the angular distribution must be nonuniform, appropriate selection of the reconstruction weights is necessary. We show that Voronoi weighting can significantly improve the fidelity of optical diffraction tomography.

Iterative eigensolver using fixed-point photonic primitive.

Photonic computing has potential advantages in speed and energy consumption yet is subject to inaccuracy due to the limited equivalent bitwidth of the analog signal. In this Letter, we demonstrate a configurable, fixed-point coherent photonic iterative solver for numerical eigenvalue problems using shifted inverse iteration. The photonic primitive can accommodate arbitrarily sized sparse matrix-vector multiplication and is deployed to solve eigenmodes in a photonic waveguide structure. The photonic iterative eigensolver does not accumulate errors from each iteration, providing a path toward implementing scientific computing applications on photonic primitives.

[Risk factors for bronchopulmonary dysplasia in twin preterm infants: a multicenter study]. / åŒèƒŽæ—©äº§å„¿æ”¯æ°”ç®¡è‚ºå‘è‚²ä¸è‰¯å±é™©å› ç´ åˆ†æžï¼šä¸€é¡¹å¤šä¸­å¿ƒç ”ç©¶.

OBJECTIVES: To investigate the risk factors for bronchopulmonary dysplasia (BPD) in twin preterm infants with a gestational age of <34 weeks, and to provide a basis for early identification of BPD in twin preterm infants in clinical practice. METHODS: A retrospective analysis was performed for the twin preterm infants with a gestational age of <34 weeks who were admitted to 22 hospitals nationwide from January 2018 to December 2020. According to their conditions, they were divided into group A (both twins had BPD), group B (only one twin had BPD), and group C (neither twin had BPD). The risk factors for BPD in twin preterm infants were analyzed. Further analysis was conducted on group B to investigate the postnatal risk factors for BPD within twins. RESULTS: A total of 904 pairs of twins with a gestational age of <34 weeks were included in this study. The multivariate logistic regression analysis showed that compared with group C, birth weight discordance of >25% between the twins was an independent risk factor for BPD in one of the twins (OR=3.370, 95%CI: 1.500-7.568, P<0.05), and high gestational age at birth was a protective factor against BPD (P<0.05). The conditional logistic regression analysis of group B showed that small-for-gestational-age (SGA) birth was an independent risk factor for BPD in individual twins (OR=5.017, 95%CI: 1.040-24.190, P<0.05). CONCLUSIONS: The development of BPD in twin preterm infants is associated with gestational age, birth weight discordance between the twins, and SGA birth.

Iterative optical diffraction tomography with embedded regularization.

Total-variation regularization is applied at each iteration of an iterative framework for optical diffraction tomography. Numerical and experimental tests are performed using various highly scattering objects, and significant improvement in reconstruction SNR are demonstrated.

Synthesis of Low-Cost and High-Performance Dual-Atom Doped Carbon-Based Materials with a Simple Green Route as Anodes for Sodium-Ion Batteries.

Sodium-ion batteries (SIBs) are promising alternatives to replace lithium-ion batteries as future energy storage batteries because of their abundant sodium resources, low cost, and high charging efficiency. In order to match the high energy capacity and density, designing an atomically doped carbonous material as the anode is presently one of the important strategies to commercialize SIBs. In this work, we report the preparation of high-performance dual-atom-doped carbon (C) materials using low-cost corn starch and thiourea (CH4N2S) as the precursors. The electronegativity and radii of the doped atoms and C are different, which can vary the embedding properties of sodium ions (Na+) into/on C. As sulfur (S) can effectively expand the layer spacing, it provides more channels for embedding and de-embedding Na+. The synergistic effect of N and S co-doping can remarkably boost the performance of SIBs. The capacity is preserved at 400 mAh g -1 after 200 cycles at 500 mA g-1; more notably, the initial Coulombic efficiency is 81%. Even at a high rate of high current of 10 A g-1, the cell capacity can still reach 170 mAh g-1. More importantly, after 3000 cycles at 1 A g-1, the capacity decay is less than 0.003% per cycle, which demonstrates its excellent electrochemical performance. These results indicate that high-performance carbon materials can be prepared using low-cost corn starch and thiourea.

Hard-Carbon Negative Electrodes from Biomasses for Sodium-Ion Batteries.

With the development of high-performance electrode materials, sodium-ion batteries have been extensively studied and could potentially be applied in various fields to replace the lithium-ion cells, owing to the low cost and natural abundance. As the key anode materials of sodium-ion batteries, hard carbons still face problems, such as poor cycling performance and low initial Coulombic efficiency. Owning to the low synthesis cost and the natural presence of heteroatoms of biomasses, biomasses have positive implications for synthesizing the hard carbons for sodium-ion batteries. This minireview mainly explains the research progress of biomasses used as the precursors to prepare the hard-carbon materials. The storage mechanism of hard carbons, comparisons of the structural properties of hard carbons prepared from different biomasses, and the influence of the preparation conditions on the electrochemical properties of hard carbons are introduced. In addition, the effect of doping atoms is also summarized to provide an in-depth understanding and guidance for the design of high-performance hard carbons for sodium-ion batteries.

High Wnt2 Expression Confers Poor Prognosis in Colorectal Cancer, and Represents a Novel Therapeutic Target in BRAF-Mutated Colorectal Cancer.

Background and Objectives: We aimed to investigate the role of Wnt2 expression in colorectal cancer (CRC) prognosis and evaluate its potential as a therapeutic target in BRAF-mutated CRC. Materials and Methods: Exactly 136 samples of formalin-fixed paraffin-embedded CRC tissue specimens were obtained from patients who underwent surgical resection for CRC. The gene mutation status of the samples was detected using fluorescence PCR. Wnt2 expression was detected using immunohistochemistry. Survival curves with high Wnt2 expression and BRAF mutations were compared using the Kaplan-Meier method. A nomogram was constructed to determine the estimated overall survival probability. We also predicted the 3-year and 5-year survival rates for patients with high Wnt2 expression and BRAF mutations. In total, 50 samples of BRAF-mutated CRC were collected and detected Wnt2 expression by immunohistochemistry. The Chi-squared test was used to analyze the association between Wnt2 expression and BRAF-mutated CRC. Results: High Wnt2 expression and BRAF mutations are associated with poor prognosis of CRC. Multivariate survival analyses indicated that high Wnt2 expression and BRAF mutations are significant independent predictors of CRC prognosis. Furthermore, high Wnt2 expression was significantly associated with BRAF-mutated CRC, and Wnt2 may be a potential therapeutic target for BRAF-mutated CRC. Conclusions: High Wnt2 expression confers poor prognosis in colorectal cancer and represents a novel therapeutic target in BRAF-mutated CRC.

Low-crosstalk mode-group demultiplexers based on Fabry-Perot thin-film filters.

Mode-group multiplexing (MGM) can increase the capacity of short-reach few-mode optical fiber communication links while avoiding complex digital signal processing. In this paper, we present the design and experimental demonstration of a novel mode-group demultiplexer (MG DeMux) using Fabry-Perot (FP) thin-film filters (TFFs). The MG DeMux supports low-crosstalk mode-group demultiplexing, with degeneracies commensurate with those of graded-index (GRIN) multimode fibers. We experimentally demonstrate this functionality by using a commercial six-cavity TFF that was intended for 100 GHz channel spaced wavelength-division multiplexing (WDM) system.

Construction of ZIF-67-On-UiO-66 Catalysts as a Platform for Efficient Overall Water Splitting.

A well-organized construction of hybrid metal-organic frameworks (MOFs) with exquisite structures is vital due to their potential applications. Herein, a novel hybrid nanostructure of UiO-66-on-ZIF-67, denoted as MZU-CoxZry (x and y represent the mass ratios of ZIF-67 and UiO-66, respectively), was successfully prepared by a simple method and showed a highly efficient and stable bifunctionality of both oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) in an alkaline medium. The MZU-Co2.5Zr1 shows remarkable OER performance with a low overpotential of 252 mV and an overpotential of 172 mV @ 10 mA/cm2 for HER in 1 mol/L KOH. With MZU-Co2.5Zr1 as the cathode and anode, the integrated water decomposition device has achieved low total potentials of 1.56 V @ 10 mA/cm2 and 1.59 V @ 30 mA/cm2, exhibiting its excellent performance of overall water splitting. Based on the experimental results, the mechanism of the superior electrocatalytic performance of MZU-CoxZry was discussed. This work supplies guidance for the rational design of non-precious composites for energy conversion.

Clinical outcome of cord blood transplantation for nine children with juvenile myelomonocytic leukemia receiving fludarabine-busulfan-cyclophosphamide-based conditioning.

BACKGROUND: Juvenile myelomonocytic leukemia (JMML) is a rare hematological malignancy in young children and can only be cured through the allogeneic stem cell transplantation. PROCEDURE: We have retrospectively analyzed the outcomes of nine children with JMML after unrelated cord blood transplantation (UCBT). RESULTS: Eight patients who have received a myeloablative conditioning regimen of fludarabine (FLU), busulfan (BU), and cyclophosphamide (CY) have gotten engraftment. None of the nine patients has relapsed following initial UCBT. Six patients are still alive and in complete remission after UCBT with a median observation time of 43 months (range: 10-80 months). CONCLUSIONS: This study shows that UCBT with FLU-BU-CY conditioning regimen can represent a suitable option for children with JMML.

Responses and comprehensive evaluation of growth characteristics of ephemeral plants in the desert-oasis ecotone to soil types.

The ecological environment of the Gurbantünggüt desert-oasis ecotone is extremely fragile. Ephemeral plants are an important part of the ecosystem and play an essential role in maintaining the ecological stability of the ecotone. However, few studies have focused on the growth, soil quality and system sustainability of ephemeral plants in different soils. This study was based on two typical soil types (grey desert soil, GS; aeolian soil, AS) in the aforementioned ecotone, considered four ephemeral plants (Tetracme recurvata, TR; Tetracme contorta, TC; Malcolmia scorpioides, MS; Isatis violascens, IV) as the research object, analysed plant characteristics and soil properties, and comprehensively evaluated the ephemeral plant system by analysing the soil quality index (SQI) and sustainability index (SI). The results showed that there were significant differences in biomass and nutrient accumulation between different ephemeral plants, which were significantly affected by soil types. In the two examined soils (GS and AS), the contents of nutrients and microbial carbon (MBC) and nitrogen (MBN) in the rhizosphere soil were higher than those in the bare soil (BS), and there were significant differences among different species. The key soil factors related to total biomass in GS and AS were also different. The SQI of ephemeral plants was significantly higher than that of the BS, and varied with soil types and plant species. The species with the highest SQI of the key factor data set in GS and AS were IV and TR, respectively. The SI analysis indicated that IV in GS and MS and IV in AS were sustainable, and the plant properties can be better used to assess the sustainability of ephemeral plant systems. In conclusion, ephemeral plants improved the soil quality and system sustainability of the study ecotone. Further, the growth of ephemeral plant and rhizosphere soil properties vary with plant species and soil types; thus, selecting suitable species for large-scale planting in different soil types is of great significance for improving the ecological stability of the ecotone.

Succession of endophytic fungi and arbuscular mycorrhizal fungi associated with the growth of plant and their correlation with secondary metabolites in the roots of plants.

BACKGROUND: To decipher the root and microbial interaction, secondary metabolite accumulation in roots and the microbial community's succession model during the plant's growth period demands an in-depth investigation. However, till now, no comprehensive study is available on the succession of endophytic fungi and arbuscular mycorrhizal fungi (AMF) with roots of medicinal licorice plants and the effects of endophytic fungi and AMF on the secondary metabolite accumulation in licorice plant's root. RESULTS: In the current study, interaction between root and microbes in 1-3 years old medicinal licorice plant's root and rhizospheric soil was investigated. Secondary metabolites content in licorice root was determined using high-performance liquid chromatography (HPLC). The composition and diversity of endophytic and AMF in the root and soil were deciphered using high-throughput sequencing technology. During the plant's growth period, as compared to AMF, time and species significantly affected the diversity and richness of endophytic fungi, such as Ascomycota, Basidiomycota, Fusarium, Cladosporium, Sarocladium. The growth period also influenced the AMF diversity, evident by the significant increase in the relative abundance of Glomus and the significant decrease in the relative abundance of Diversispora. It indicated a different succession pattern between the endophytic fungal and AMF communities. Meanwhile, distance-based redundancy analysis and Mantel tests revealed root's water content and secondary metabolites (glycyrrhizic acid, liquiritin, and total flavonoids), which conferred endophytic fungi and AMF diversity. Additionally, plant growth significantly altered soil's physicochemical properties, which influenced the distribution of endophytic fungal and AMF communities. CONCLUSIONS: This study indicated a different succession pattern between the endophytic fungal and AMF communities. During the plant's growth period, the contents of three secondary metabolites in roots increased per year, which contributed to the overall differences in composition and distribution of endophytic fungal and AMF communities. The endophytic fungal communities were more sensitive to secondary metabolites than AMF communities. The current study provides novel insights into the interaction between rhizospheric microbes and root exudates.

Amorphous Yolk-Shelled ZIF-67@Co3(PO4)2 as Nonprecious Bifunctional Catalysts for Boosting Overall Water Splitting.

It is challenging to generate inexpensive and noble metal-free catalysts for efficient overall water splitting (OWS). To achieve this goal, suitable tuning of the structure and composition of electrocatalytic materials is a promising approach that has attracted much attention in recent years. Herein, novel hybrid amorphous ZIF-67@Co3(PO4)2 electrocatalysts with yolk-shell structures were prepared using a reflux method. It is demonstrated that yolk-shelled ZIF-67@Co3(PO4)2 is not only an active catalyst for the hydrogen evolution reaction (HER) but also an efficient catalyst for the oxygen evolution reaction (OER). The optimized composite electrode showed superior performance with low overpotentials of 73 and 334 mV @ 10 mA·cm-2 toward HER and OER, respectively, and a low potential of 1.62 V @ 10 mA·cm-2 and 1.66 V @ 30 mA·cm-2 in a practical OWS test under alkaline conditions. N-O bonds were formed to connect the two components of ZIF-67 and Co3(PO4)2 in the composite ZIF-67@Co3(PO4)2, which indicates that the two components are synergistic but not isolated, and this synergistic effect may be one of the important reasons to boost the oxygen and hydrogen evolution performances of the hybrid. Based on experimental data, the high electrocatalytic performance was inferred to be related to the unique structure of ZIF-67, tuning the ability of Co3(PO4)2 and synergism between ZIF-67 and Co3(PO4)2. The preparation strategy reported herein can be extended for the rational design and synthesis of cheap, active, and long-lasting bifunctional electrocatalysts for OWS and other renewable energy devices.

Optimization-based optical diffraction tomography using iODT initialization.

Optical diffraction tomography (ODT) is a label-free and noninvasive technique for biological imaging. However, ODT is only applicable to weakly scattering objects. To extend ODT to the multiple-scattering regime, more advanced inversion algorithms have been developed, including optimization-based ODT (Opti-ODT) and iterative ODT (iODT). In this paper, we propose a combined strategy, namely, an iODT initialization for Opti-ODT, based on the observed complementarity of their individual advantages. This study numerically demonstrates that under this combined strategy, the reconstruction can accurately converge to a better local minimum, especially in the case of multiply scattering objects with large optical path differences.

High-throughput sequencing analysis of the rhizosphere arbuscular mycorrhizal fungi (AMF) community composition associated with Ferula sinkiangensis.

BACKGROUND: Ferula sinkiangensis is an increasingly endangered medicinal plant. Arbuscular mycorrhiza fungi (AMF) are symbiotic microorganisms that live in the soil wherein they enhance nutrient uptake, stress resistance, and pathogen defense in host plants. While such AMF have the potential to contribute to the cultivation of Ferula sinkiangensis, the composition of AMF communities associated with Ferula sinkiangensis and the relationship between these fungi and other pertinent abiotic factors still remains to be clarified. RESULTS: Herein, we collected rhizosphere and surrounding soil samples at a range of depths (0-20, 20-40, and 40-60 cm) and a range of slope positions (bottom, middle, top). These samples were then subjected to analyses of soil physicochemical properties and high-throughput sequencing (Illumina MiSeq). We determined that Glomus and Diversispora species were highly enriched in all samples. We further found that AMF diversity and richness varied significantly as a function of slope position, with this variation primarily being tied to differences in relative Glomus and Diversispora abundance. In contrast, no significant relationship was observed between soil depth and overall AMF composition, although some AMF species were found to be sensitive to soil depth. Many factors significantly affected AMF community composition, including organic matter content, total nitrogen, total potassium, ammonium nitrogen, nitrate nitrogen, available potassium, total dissolvable salt levels, pH, soil water content, and slope position. We further determined that Shannon diversity index values in these communities were positively correlated with total phosphorus, nitrate-nitrogen levels, and pH values (P < 0.05), whereas total phosphorus, total dissolvable salt levels, and pH were positively correlated with Chao1 values (P < 0.05). CONCLUSION: In summary, our data revealed that Glomus and Diversispora are key AMF genera found within Ferula sinkiangensis rhizosphere soil. These fungi are closely associated with specific environmental and soil physicochemical properties, and these soil sample properties also differed significantly as a function of slope position (P < 0.05). Together, our results provide new insights regarding the relationship between AMF species and Ferula sinkiangensis, offering a theoretical basis for further studies of their development.

Root-associated endophytic bacterial community composition and structure of three medicinal licorices and their changes with the growing year.

BACKGROUND: The dried roots and rhizomes of medicinal licorices are widely used worldwide as a traditional medicinal herb, which are mainly attributed to a variety of bioactive compounds that can be extracted from licorice root. Endophytes and plants form a symbiotic relationship, which is an important source of host secondary metabolites. RESULTS: In this study, we used high-throughput sequencing technology and high-performance liquid chromatography to explore the composition and structure of the endophytic bacterial community and the content of bioactive compounds (glycyrrhizic acid, liquiritin and total flavonoids) in different species of medicinal licorices (Glycyrrhiza uralensis, Glycyrrhiza glabra, and Glycyrrhiza inflata) and in different planting years (1-3 years). Our results showed that the contents of the bioactive compounds in the roots of medicinal licorices were not affected by the species, but were significantly affected by the main effect growing year (1-3) (P < 0.05), and with a trend of stable increase in the contents observed with each growing year. In 27 samples, a total of 1,979,531 effective sequences were obtained after quality control, and 2432 effective operational taxonomic units (OTUs) were obtained at 97% identity. The phylum Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes, and the genera unified-Rhizobiaceae, Pseudomonas, Novosphingobium, and Pantoea were significantly dominant in the 27 samples. Distance-based redundancy analysis (db-RDA) showed that the content of total flavonoids explained the differences in composition and distribution of endophytic bacterial communities in roots of cultivated medicinal liquorices to the greatest extent. Total soil salt was the most important factor that significantly affected the endophytic bacterial community in soil factors, followed by ammonium nitrogen and nitrate nitrogen. Among the leaf nutrition factors, leaf water content had the most significant effect on the endophytic bacterial community, followed by total phosphorus and total potassium. CONCLUSIONS: This study not only provides information on the composition and distribution of endophytic bacteria in the roots of medicinal licorices, but also reveals the influence of abiotic factors on the community of endophytic bacteria and bioactive compounds, which provides a reference for improving the quality of licorice.

Iterative optical diffraction tomography for illumination scanning configuration.

Optical diffraction tomography (ODT) is used to reconstruct refractive-index distributions from multiple measurements in the object rotating configuration (ORC) or the illumination scanning configuration (ISC). Because of its fast data acquisition and stability, ISC-based ODT has been widely used for biological imaging. ODT typically fails to reconstruct multiply-scattering samples. The previously developed iterative ODT (iODT) was for the multiply-scattering objects in ORC, and could not be directly applied to ISC. To resolve this mismatch, we developed an ISC update and numerically demonstrated its accuracy. With the same prior knowledge, iODT-ISC outperforms conventional ODT in resolving the missing-angle problem.

Reconstructing complex refractive-index of multiply-scattering media by use of iterative optical diffraction tomography.

Conventional optical diffraction tomography (ODT) techniques fail in the presence of multiple scattering, and the problem becomes even more challenging when the medium is also lossy. Iterative ODT (iODT), which was shown recently to be more tolerant to multiple scattering than conventional ODT, is here augmented with an error-subtraction (ES) module. Numerical results demonstrate the accuracy and efficiency of iODT with ES for reconstructing multiply-scattering objects with complex refractive index.

Few-mode frequency-modulated LiDAR receivers.

We present a few-mode frequency-modulated receiver for light detection and ranging (LiDAR). We show that using a few-mode local oscillator (LO) with spatial modes at different frequencies at the receiver can significantly improve the performance of the LiDAR detection range. A preferred receiver architecture features LO modes with unequal frequency separations based on optical orthogonal codes (OOC) to allow range detection via cross correlation. The required signal-to-noise ratio (SNR) for the frequency-modulated continuous wave (FMCW) LiDAR decreases with the number of LO modes. This receiver can have a potential impact in the area of automotive LiDARs.

Scalable Hermite-Gaussian mode-demultiplexing hybrids.

We propose a Hermite-Gaussian (HG) mode-demulti-plexing hybrid (MDH) for coherent detection of mode-division multiplexed signals. The MDH, which performs multiple functionalities, including demultiplexing, local oscillator splitting, and optical 90-deg mixing, is realized based on the multi-plane light conversion technique. An isosceles right triangle output layout is employed to reduce the number of phase masks to fewer than the number of modes, significantly simplifying the construction of the MDH. A 10-Hermite-Gaussian (HG) mode MDH with only five phase masks is demonstrated by numerical simulation, achieving an insertion loss (IL) and mode dependent loss as low as ${-}{2.3}$-2.3 and 1.7 dB, respectively. The IL was further reduced to ${-}{1.5}\;{\rm dB}$-1.5dB through optimization of MDH parameters, such as the beam waists of the input HG modes and the output spots.