RESUMEN
Plant polysaccharides exhibit many biological activities that are remarkably affected by molecular size and structures. This study aimed to investigate the degradation effect of ultrasonic-assisted Fenton reaction on the Panax notoginseng polysaccharide (PP). PP and its three degradation products (PP3, PP5, and PP7) were obtained from optimized hot water extraction and different Fenton reaction treatments, respectively. The results showed that the molecular weight (Mw) of the degraded fractions significantly decreased after treatment with the Fenton reaction. But the backbone characteristics and conformational structure were similar between PP and PP-degraded products, which was estimated by comparing monosaccharides composition, functional group signals in FT-IR spectra, X-ray differential patterns, and proton signals in 1H NMR. In addition, PP7, with an Mw of 5.89 kDa, exhibited stronger antioxidant activities in both the chemiluminescence-based and HHL5 cell-based methods. The results indicated that ultrasonic-assisted Fenton degradation might be used to improve the biological activities of natural polysaccharides by adjusting the molecular size.
Asunto(s)
Antioxidantes , Panax notoginseng , Antioxidantes/química , Panax notoginseng/química , Ultrasonido , Espectroscopía Infrarroja por Transformada de Fourier , Polisacáridos/química , Oligosacáridos , Peso MolecularRESUMEN
Camellia oleifera leaf is a rich source of polyphenols. In this study, 50 polyphenolic compounds from C. oleifera leaves was identified by UHPLC-Q-TOF-MS/MS. Accordingly, COSMO-RS was used in the design of deep eutectic solvents (DESs) to extract those polyphenols. 17 types of choline chloride (ChCl)-based DESs molecules (ChCl-acid, ChCl-sugar, ChCl-alcohol, ChCl-amine and amide) were synthetized into virtual cluster molecules with Materials Studio software. They were used to determine the activity coefficients with the standard compounds. The results showed that the amine and amide-based DESs exhibited outstanding dissolution effects. Additionally, ChCl-acetamide was selected as the solvent in response surface methodology to optimize the ultrasound-assisted DES extraction process parameters, including ultrasonic power, ultrasonic time, and liquid-solid ratio, resulting in an improved total phenolic content of 131.63 ± 0.85 mg GAE/g. This study developed a system utilizing UHPLC-Q-TOF-MS/MS to acquire specific substances required for COSMO-RS calculations.