RESUMEN
A reverse-phase (RP)-HPLC method is reported for determining L-tyrosine, p-octopamine, synephrine, tyramine and hordenine as chemical markers of the species Citrus aurantium in raw material, dry extracts and phytotherapeutic herbal formulations. Using RP-HPLC with diode array detection (DAD) and gradient elution, the amines were determined in 12 different products from different Brazilian states labelled as containing C. aurantium. The presence of the amines was confirmed by mass spectrometry using electrospray ionisation (ESI-MS/MS). This RP-HPLC method allowed the separation of the amines from complex mixtures containing caffeine, ephedrine, salicin and other raw materials (e.g. Garcinia camboja, Phaseolus vulgaris, Caralluma fimbriata, Cassia nomane, Ephedra sp. and Cordia ecalyculata). The method proved useful and selective for inspecting herbal medicines containing p-synephrine and structural analogues. The herbal products analysed had a p-synephrine content ranging from 0.005 to 4.0% (w/w).
Asunto(s)
Aminas Biogénicas/análisis , Cromatografía Líquida de Alta Presión/métodos , Citrus/química , Preparaciones Farmacéuticas/química , Estándares de Referencia , Espectrometría de Masa por Ionización de Electrospray , Espectrometría de Masas en TándemRESUMEN
ABSTRACT The phenolic content of the medicinal species Connarus perrottetti var. angustifolius Radlk., Connaraceae, Cecropia obtuse Trécul, Cecropia palmata Willd., Urticaceae; and Mansoa alliacea (Lam.) A.H.Gentry, Bignoniaceae, collected in three different years was evaluated. Plant infusions and hydroalcoholic, butanol and ethyl acetate extracts were analyzed by high-performance liquid chromatography with diode array detection. In order to endorse these results, analysis by electrospray mass spectrometry was also performed. Were identified: gallic acid, catechin, caffeic acid, ferulic acid, rutin, quercitrin and resveratrol. C. perrottetti showed greater diversity of polyphenols. M. alliacea had the higher concentration of caffeic acid even though it was found in all species. Catechin was the major antioxidant, but was not detected in M. alliacea. However, we discuss the popular use of these species, as well as their phenolic constitution and the interannual distribution of phenolic compounds.