Insight into Bioaccumulation of Decabromodiphenyl Ethane in Eisenia fetida Increased by Microplastics.

The rise of electronics inevitably induced the co-pollution of novel brominated flame retardants (NBFRs) and microplastics (MPs). However, studies on how they interact to influence their bioavailability are scarce. Here, we explored the influence mechanism of acrylonitrile butadiene styrene (ABS)-MPs on the bioaccumulation of decabromodiphenyl ethane (DBDPE) in soil-earthworm microcosms. The influence exhibited a temporal pattern characterized by short-term inhibition and long-term promotion. After 28 days of exposure, DBDPE bioaccumulation in a co-exposure (10 mg kg-1 DBDPE accompanied by 1000 mg kg-1 ABS-MPs) was 2.61 times higher than that in a separate exposure. The adsorption process in the soil, intestines, and mucus introduced DBDPE-carried MPs, which had a higher concentration of DBDPE than the surrounding soil and directly affected the bioavailability of DBDPE. MP-pre-exposure (100, 1000, and 10000 mg kg-1) reduced epidermal soundness, mucus secretion, and worm cast production. This eventually promoted the contact between earthworm and soil particles and enhanced the DBDPE of earthworm tissue by 6%-61% in the next DBDPE-postexposure period, confirming that MPs increased DBDPE bioaccumulation indirectly by impairing the earthworm health. This study indicates that MPs promoted DBDPE bioaccumulation via adsorption and self-toxicity, providing new insight into the combined risk of MPs and NBFRs.

Decabromodiphenyl Ethane Mainly Affected the Muscle Contraction and Reproductive Endocrine System in Female Adult Zebrafish.

The novel brominated flame retardant decabromodiphenyl ethane (DBDPE) has become a widespread environmental pollutant. However, the target tissue and toxicity of DBDPE are still not clear. In the current study, female zebrafish were exposed to 1 and 100 nM DBDPE for 28 days. Chemical analysis revealed that DBDPE tended to accumulate in the brain other than the liver and gonad. Subsequently, tandem mass tag-based quantitative proteomics and parallel reaction monitoring verification were performed to screen the differentially expressed proteins in the brain. Bioinformatics analysis revealed that DBDPE mainly affected the biological process related to muscle contraction and estrogenic response. Therefore, the neurotoxicity and reproductive disruptions were validated via multilevel toxicological endpoints. Specifically, locomotor behavioral changes proved the potency of neurotoxicity, which may be caused by disturbance of muscular proteins and calcium homeostasis; decreases of sex hormone levels and transcriptional changes of genes related to the hypothalamic-pituitary-gonad-liver axis confirmed reproductive disruptions upon DBDPE exposure. In summary, our results suggested that DBDPE primarily accumulated in the brain and evoked neurotoxicity and reproductive disruptions in female zebrafish. These findings can provide important clues for a further mechanism study and risk assessment of DBDPE.

Bioconcentration, Biotransformation, and Thyroid Endocrine Disruption of Decabromodiphenyl Ethane (Dbdpe), A Novel Brominated Flame Retardant, in Zebrafish Larvae.

The brominated flame retardant decabromodiphenyl ethane (DBDPE), an alternative to decabrominated diphenyl ether (BDE209), has become a widespread environmental contaminant, but its possible toxic effects to wildlife remain unknown. Using zebrafish as a model, we investigated the bioconcentration and impact of DBDPE on thyroid endocrine function after water-borne exposure, compared to BDE209. Zebrafish embryos were exposed to DBDPE or BDE209 (0, 3, 10, 30, 100, 300 nM) for 6 or 14 days. Chemical analysis revealed that DBDPE and BDE209 were bioconcentrated in zebrafish larvae, with similar magnitudes of accumulated concentrations. Based on screened by chromatograms, at least seven unknown compounds were observed in DBDPE-treated larvae, indicating biotransformation of the chemical. Significant increases in whole body content of triiodothyronine (T3) and thyroxine (T4) were detected in DBDPE-treated larvae, but decreased in BDE209-treated groups. Alterations in gene transcription along the related hypothalamic-pituitary-thyroid (HPT) axis were observed. Furthermore, the binding and transport protein transthyretin (TTR) was significantly increased in DBDPE exposure groups. Histological examination and stereological analysis showed no obvious pathological changes in the thyroid gland. The present study demonstrates for the first time the bioavailability, biotransformation and thyroid endocrine disruption associated with DBDPE exposure in fish. Further studies are warranted to identify the metabolites of DBDPE and to define its environmental risks to aquatic organisms.

Combined exposure to decabromodiphenyl ether and nano zero-valent iron aggravated oxidative stress and interfered with metabolism in earthworms.

Decabromodiphenyl ether (BDE-209) is a common brominated flame retardant in electronic waste, and nano zero-valent iron (nZVI) is a new material in the field of environmental remediation. Little is known about how BDE-209 and nZVI combined exposure influences soil organisms. During the 28 days study, we determined the effects of single and combined exposures to BDE-209 and nZVI on the oxidative stress and metabolic response of earthworms (Eisenia fetida). On day 7, compared to CK, malondialdehyde (MDA) content increased in most combined exposure groups. To remove MDA and reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were induced in most combined exposure groups. On day 28, compared to CK, the activities of SOD and CAT were inhibited, while POD activity was significantly induced, indicating that POD plays an important role in scavenging ROS. Combined exposure to BDE-209 and nZVI significantly affected amino acid biosynthesis and metabolism, purine metabolism, and aminoacyl-tRNA biosynthesis pathways, interfered with energy metabolism, and aggravated oxidative stress in earthworms. These findings provide a basis for assessing the ecological impacts of using nZVI to remediate soils contaminated with BDE-209 from electronic waste.

Five coexisting brominated flame retardants in a water-sediment-Vallisneria system: Bioaccumulation and effects on oxidative stress and photosynthesis.

The pollution of various brominated flame retardants (BFRs) is concurrence, while their environmental fate and toxicology in water-sediment-submerged plant systems remain unclear. In this study, Vallisneria natans plants were co-exposed to 2,3,4,5,6-pentabromotoluene (PBT), hexabromobenzene (HBB), 1,2-bis (2,4,6-tribromophenoxy) ethane (BTBPE), decabromodiphenyl ether (BDE209), and decabromodiphenyl ethane (DBDPE). The ∑BFRs concentration in the root was 2.15 times higher than that in the shoot. Vallisneria natans accumulated more BTBPE and HBB in 0.2, 1, and 5 mg/kg treatments, while they accumulated more DBDPE and BDE209 in 25 and 50 mg/kg treatments. The bioaccumulation factors in the shoot and root were 1.08-96.95 and 0.04-0.70, respectively. BFRs in sediments had a more pronounced effect on bioaccumulation levels than BFRs in water, and biotranslocation was another potential influence factor. The SOD activity, POD activity, and MDA content were significantly increased under co-exposure. The DBDPE separate exposure impacted the metabolism of substances and energy, inhibited mismatch repair, and disrupted ribosomal functions in Vallisneria natans. However, DBDPE enhanced their photosynthesis by upregulating the expression level of genes related to the light reaction. This study provides a broader understanding of the bioaccumulation and toxicity of BFRs in submerged plants, shedding light on the scientific management of products containing BFRs.

Contamination characteristics and dietary intake risk of brominated flame retardants in fishes around a typical e-waste dismantling site in Southern China.

Polybrominated diphenyl ethers (PBDEs) and their substitutes, novel brominated flame retardants (NBFRs), are ubiquitously present in the aquatic environment of electronic waste (e-waste) dismantling region, leading to their inevitable absorption and accumulation by aquatic organisms, which can be transferred to human via directly aquatic product consumption or through food chain, thereby posing potential health risks. This study focused on fish samples from Guiyu and its surrounding areas, and found the total PBDEs concentrations were 24-7400 ng/g lw (mean: 1800 ng/g lw) and the total NBFRs concentrations were 14 to 2300 ng/g lw (mean: 310 ng/g lw). Significant positive correlations were found among PBDE congeners, among different NBFRs, and between NBFRs and commercial PBDEs that they replace. ΣPBDEs and ΣNBFRs in the intestine were 620-350,000 and 91-81,000 ng/g lw (mean: 83000 and 12,000 ng/g lw, respectively), significantly exceeding those in the gills, where ΣPBDEs and ΣNBFRs were 14-37,000 and 39-45,000 ng/g lw (mean: 9200 and 2400 ng/g lw, respectively). The ΣPBDEs and ΣNBFRs showed no non-carcinogenic risks to the target population through dietary intake. Despite the significantly higher daily intake of decabromodiphenyl ethane (DBDPE) compared to decabromodiphenyl ether (BDE209), the non-carcinogenic risk associated with BDE209 remained higher than that of DBDPE. Our findings can assist researchers in understanding the presence of BFRs in aquatic organisms, inhabiting e-waste dismantling areas, and in evaluating the associated health risks posed to humans through dietary exposure.

Aspirin altered antibiotic resistance genes response to sulfonamide in the gut microbiome of zebrafish.

Pharmaceuticals are widespread in aquatic environments and might contribute to the prevalence of antibiotic resistance. However, the co-effect of antibiotics and non-antibiotic pharmaceuticals on the gut microbiome of fish is poorly understood. In this study, we characterized the variation of the zebrafish gut microbiome and resistome after exposure to sulfamethoxazole (SMX) and aspirin under different treatments. SMX contributed to the significant increase in the antibiotic resistance genes (ARGs) richness and abundance with 46 unique ARGs and five mobile genetic elements (MGEs) detected. Combined exposure to SMX and aspirin enriched total ARGs abundance and rearranged microbiota under short-term exposure. Exposure time was more responsible for resistome and the gut microbiome than exposure concentrations. Perturbation of the gut microbiome contributed to the functional variation related to RNA processing and modification, cell motility, signal transduction mechanisms, and defense mechanisms. A strong significant positive correlation (R = 0.8955, p < 0.001) was observed between total ARGs and MGEs regardless of different treatments revealing the key role of MGEs in ARGs transmission. Network analysis indicated most of the potential ARGs host bacteria belonged to Proteobacteria. Our study suggested that co-occurrence of non-antibiotics and antibiotics could accelerate the spread of ARGs in gut microbial communities and MGEs played a key role.

Dynamics and mechanisms of bioaccumulation and elimination of nonylphenol in zebrafish.

Nonylphenol (NP) has been widely concerned for its endocrine disrupting effects. In this study, we investigated the accumulation and elimination of NP for the whole body and trunk of zebrafish (Danio rerio). The results show that the LC50 values of NP in zebrafish ranged from 474 µg·L-1 (24-h exposure) to 238 µg·L-1 (96-h exposure). Meanwhile, the NP concentrations in zebrafish during the depuration stage fitted the first-order kinetic model well, and the depuration rate constant (K2) was reduced from 0.412 d-1 to 0.2827 d-1 with higher NP. The half-life (t1/2) of NP was 1.75-2.45 d in the whole fish and 0.56-0.86 d in the trunk under low to high NP, respectively. Both the accumulation and elimination of NP in trunk were faster than those in whole fish, indicating the preferential transfer from viscera to muscle and rapidly diffusion in reverse. The bioconcentration factors (BCFSS) of NP were 104-112 L·kg-1 in whole body and 76-104 L·kg-1 in trunk, respectively, suggesting that the muscle was a major position for NP storage.

Occurrence, spatial distribution, and risk assessment of brominated flame retardants in farmland soils of typical provinces in China.

In the present study, we investigated the occurrence, distribution, and potential risks of 4 brominated flame retardants in farmland soils across 18 provinces of China. The total mean concentrations of the BFRs were in order as DBDPE > BDE209 > HBB > TBB. DBDPE concentration was highest at 177.208 ng/kg, revealing its long-term use and persistence across the study areas. In parts of China, DBDPE was highest in the south (Sichuan, Shaanxi and Guangdong provinces), BDE209 was highest in the south (Sichuan province) and north (Jilin province), while HBB was highest in the south (Sichuan province) and east (Anhui and Zhejiang provinces) of China. Comparisons of the results in this study to other reported studies in different regions indicated that the studied BFRs concentrations were higher in the studied provinces of China. Pearson correlation between BFRs revealed both positive and negative associations within the BFRs groups and between BFRs and soil properties (SOM, CEC, pH, EC and PS%). The mean hazard quotients (HQ) of ecological risks ranged from 8.76 × 10-6 to 1.16 × 10-2 (HQ < 1) while non-carcinogenic human health risk evaluation for adults ranged from 7.05 × 10-7 - 7.48 × 10-4 (HQ < 1) and for children 2.99 × 10-4 - 4.30 × 10-2 (HQ < 1). Although the risk evaluations of BFRs from farmland soils in this study were low, the results serve as useful indicators of potential cumulative and long-term threats of BFR to rural areas where there is a high conversion of agricultural lands to non-agricultural use.

Legacy and novel brominated flame retardants in a lab-constructed freshwater ecosystem: Distribution, bioaccumulation, and trophic transfer.

The extensive utilization of both legacy and novel brominated flame retardants (BFRs) leads to high environmental concentrations, which would be bioaccumulated by organisms and further transferred through the food webs, causing potential risks to humans. In this study, five BFRs, that showed high detection frequencies and concentrations in sediments from an e-waste dismantling site in Southern China, namely 2,3,4,5,6-pentabromotoluene (PBT), hexabromobenzene (HBB), 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE), decabromodiphenyl ethane (DBDPE), and decabromodiphenyl ether (BDE209), were selected as target pollutants in the lab-constructed aquatic food web as part of a micro-ecosystem, to investigate their distribution, bioaccumulation, and trophic transfer patterns. The significant correlations between different samples in the food web indicated that the dietary uptake appeared to influence the levels of BFRs in organisms. Significant negative correlations were observed between the trophic level of organisms and the lipid-normalized concentrations of BTBPE and DBDPE, indicating the occurrence of trophic dilution after 5-month exposure. However, the average values of bioaccumulation factors (BAFs) were from 2.49 to 5.17 L/kg, underscoring the importance of continued concern for environmental risks of BFRs. The organisms occupying higher trophic levels with greater bioaccumulation capacities may play a pivotal role in determining the trophic magnification potentials of BFRs. This research provides a helpful reference for studying the impacts of feeding habits on bioaccumulation and biomagnification, as well as for identifying the fate of BFRs in aquatic environment.

Fate and toxicity of legacy and novel brominated flame retardants in a sediment-water-clam system: Bioaccumulation, elimination, biotransformation and structural damage.

Due to the characteristics of persistent organic pollutants (POPs), some legacy brominated flame retardants (LBFRs) were prohibited from use, and then gradually replaced by novel brominated flame retardants (NBFRs). However, till now little research focused on the effects of NBFRs on the benthos. In the present study, 0.5, 5, and 50 mg/kg dw of pentabromotoluene (PBT), hexabromobenzene (HBB), 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE), decabromodiphenyl ethane (DBDPE) and decabromodiphenyl ether (BDE209) were added into sediments to test freshwater clams (Corbicula fluminea). In the 35-day exposure experiment, C. fluminea had different enrichment behaviors in three treatment groups. It was conjectured that in the lower dose group, the clams ingested contaminants and tended to be stable over time. While in higher dose groups, the clams were induced by the chemicals, leading to the changes in physiological activities so that the concentrations showed a downward trend first and then went up. The half-lives of contaminants in freshwater clams were between 0.911 and 11.6 days. DBDPE showed stronger bioaccumulation ability than BDE209 in this study. Parabolic relationships were observed between log BSAF and log Kow values in clam tissues. Debromination, hydroxylation, and methoxylated products were detected. Additionally, the gill samples of C. fluminea exposed to 50 mg/kg dw of single substance were observed by scanning electron microscope (SEM), indicating that the adhesions, tissue hyperplasia, and messy cilia occurred on the surface. Our research potentially contributes to further evaluations of the environmental risks posed in sediments contaminated by PBT, HBB, BTBPE, DBDPE, and BDE209, particularly the benthic organisms.

Cytotoxicity profiling of decabromodiphenyl ethane to earthworm (Eisenia fetida): Abnormity-recovery-dysregulation physiological pattern reflects the coping mechanism.

Response of terrestrial invertebrates to decabromodiphenyl ethane (DBDPE) is an emerging field of research nowadays, while cytotoxicity of DBDPE and self-defense strategies of invertebrates are poorly understood. In this study, earthworms (Eisenia fetida) were incubated in the DBDPE-spiked soil system (10, 30, 50, 70, and 100 mg kg-1 dw) for 28-d uptake. The bioaccumulation and distribution of DBDPE, a series of biomarkers associated with lysosomes/mitochondria, and the apoptosis rate of coelomocytes have been evaluated on the 7th, 14th, 21th, and 28th day. At experimental endpoint, the autophagy/apoptosis phenomena have been observed under transmission electron microscopy and the expression levels of six target genes have been explored. Findings in this paper revealed that: bioaccumulation factors decreased with the incremental DBDPE concentrations in the soil. Intestinal ingestion, but not epidermal contact predominated the absorption of DBDPE. The fluctuations of biomarkers and the apoptosis rate were described as the "abnormity-recovery-dysregulation" pattern. Intense oxidative stress, energy demands, membrane-system damage, pathological organelles, and apoptosis were observed in the treated groups. Conclusively, the cytotoxicity of DBDPE initiated the mitochondrial apoptosis pathway which affected the physiological status of lysosomes, autophagy, and the expression of genes. The coping mechanisms of Eisenia fetida to DBDPE included activating mitochondrial electron transport processes, reorganizing actin cytoskeleton, and initiating autophagy. Earthworms resisted the cytotoxicity of DBDPE to a certain extent, while long-term exposure still resulted in apoptosis of coelomocytes. This study works as a laboratory simulation for the environmental safety evaluation of DBDPE and the detoxification mechanisms for earthworm.

Brominated flame retardants (BFRs) in sediment from a typical e-waste dismantling region in Southern China: Occurrence, spatial distribution, composition profiles, and ecological risks.

Our study evaluated the current occurrence, composition, and spatial distribution of eight congeners of polybrominated diphenyl ethers (PBDEs) and seven novel brominated flame retardants (NBFRs) in sediment from Guiyu, a typical e-waste dismantling region in China. PBDEs levels ranged from 0.345 to 401,000 ng/g dw and NBFRs levels ranged from 0.581 to 73,100 ng/g dw. Almost all sediment samples contained high levels of BDE-209 and DBDPE, and the ratio of DBDPE/BDE-209 in sediments ranged from 0.0814 to 2.80 (mean: 0.879). The concentration and composition profiles for BFRs in sediments from both mainstream and tributaries of two major rivers in Guiyu reach (and adjacent downstream locations) differed significantly from those far from Guiyu town. Whereas the high presence of BFRs in Guiyu reflected the historical crude e-waste dismantling activities in the region; the locations far from Guiyu town were likely to receive BFRs from atmospheric deposition, not originated from the region, as BFRs in water-sediment are known to be able to migrate a limited distance along the river. Ecological risk assessment revealed that the low brominated congeners of PBDEs and BDE-209 posed an unacceptable risk to the sedimentary life at multiple locations. Our results updated our knowledge of BFRs contamination in Guiyu, suggesting the necessity of continuous source monitoring, control procedures, and sediment cleanup for BFRs.

Bioaccumulation, elimination and metabolism in earthworms and microbial indices responses after exposure to decabromodiphenyl ethane in a soil-earthworm-microbe system.

As a novel brominated flame retardant (NBFR), decabromodiphenyl ethane (DBDPE) has been poorly understood for the environmental fate and toxicity in terrestrial invertebrates. For the first time, the bioaccumulation, elimination, metabolism and detoxification of DBDPE in earthworms as well as its potential impacts on soil microbes were investigated. The results showed much higher DBDPE concentrations in casts than in earthworms. The bioaccumulation factor (BAF) and elimination rate constant (ke) values were 0.028-0.213 (gdw, worm/gdw, soil) and 0.323-0.452 (day-1), respectively. The detoxifying enzymes (CYP450 and GST) could be induced by DBDPE within the range of exposure dosage, and the activities were significantly increased at 21 d (p < 0.05). The results were identified by GC-ECNI-MS, and it showed that at least eleven unknown peaks were separately observed in the earthworms, which were the biotransformation products of DBDPE in earthworms. Additionally, the damages, including skin shrinkage, setae impairment, and intercellular vacuolization, were clearly observed by SEM/TEM. Based on these data, DBDPE could accumulate in earthworms, yet, with low bioaccumulation ability. Moreover, DBDPE exposure resulted in minimal harmful impacts on microbial activities including microbial biomass C (MBC), Microbial basal respiration (MBR), Urease (US) activity and fluorescein diacetate hydrolase (FDA) activity (p < 0.05). Our findings would provide some essential information for interpreting the ecological risks of DBDPE in soil.

Comprehensive adsorption behavior and mechanism of PFOA and PFCs in various subsurface systems in China.

The adsorption-desorption performance of perfluorooctanoic acid (PFOA), one of the environmentally persistent pollutants which is refractory to degrade in soil, was investigated and reported. The adsorption-desorption process of PFOA was firstly conducted using different fractions (sand, coarse silt and fine silt) of soil collected from Shanghai, China. More than 50% of PFOA (2.0 mg/L) could be adsorbed by soils while only less than 10% of which could be desorbed once contamination occurs. The kinetics and particle diffusion rates of PFOA in different fractions of soil were calculated and analyzed in detail. Apart from this, the retention of short-chained PFCs, which can be generated as degradation products of PFOA, were also measured. In single solute systems, the adsorption of pollutants in soils dramatically increased as the chain length of PFCs grew longer. Similarly, in mixed solutions, preferential adsorption of longer-chained PFCs over shorter chains in soils were sited, attributable to the stronger hydrophobicity of the pollutants. However, the desorption of them performed in reverse, where the desorption rates of longer-chained PFCs were far lower than those of shorter ones. Furthermore, influencing factors including pH, temperature and co-existing matters were studied during the adsorption process. After comprehending the adsorption behavior of PFOA in soil fractions, the situation of the adsorption of PFOA in various soils chosen from nine provinces in China was investigated and compared. There was an obvious discrepancy, whether it be from the rate or the amount of adsorption of PFOA (approximately 10%), in the nine different soils. Finally, a multiple linear regressive equation was employed to sort influencing parameters which are prone to affect the adsorption of PFOA in soils, the contribution of these are provided in order of relevance. These results demonstrate the adsorption performance and behavior of PFOA and PFCs in different soils, which can be utilized as a scientific reference for maximizing remediation of PFOA polluted sites in the future.

Characteristics of legacy and novel brominated flame retardants in water and sediment surrounding two e-waste dismantling regions in Taizhou, eastern China.

A total of 51 water and 43 sediment samples were collected from the locations surrounding the two e-waste dismantling zones in Taizhou, the Fengjiang resource recycling industrial zone (FJ, shut down in 2017) and the Taizhou resource recycling base (TZ, newly constructed in recent years). The concentrations of polybrominated diphenyl ethers (PBDEs) ranged from 1.7 to 44 ng/L in water and from not detected (nd) to 7100 ng/g in sediment. Novel brominated flame retardants (NBFRs) ranged from 0.29 to 1.6 ng/L in water, and from nd to 5300 ng/g in sediment. The levels of PBDEs and NBFRs in the water were comparable between FJ and TZ, while their concentrations were higher in the sediment from FJ than those from TZ. The levels of BDE-28, BDE-153, pentabromotoluene (PBT), pentabromobenzene (PBB), ∑PBDEs and ∑BFRs in the water from FJ or TZ were found to be significantly negatively associated with the distance from the zone center. However, in the sediments from FJ and TZ, the BFRs levels did not decrease from the center to the outer regions. BDE-209 and decabromodiphenyl ethane (DBDPE) were predominant in the sediments and the ratio of DBDPE/BDE-209 were as high as 5.6 (mean: 0.97). The mass burden of PBDEs, BDE-209, DBDPE, 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE), and HBB in the riverine sediments in Luqiao District was 829, 787, 363, 85, and 61 kg, respectively. The ecological risk assessment revealed that BDE-99 posed an unacceptable risk to aquatic life at 86% of the locations. The hazard quotients for penta-BDE, BDE-209, and HBB exceeded one for 30%, 28%, and 2.3% of the sediment samples, respectively.

Exploring the environmental fate of novel brominated flame retardants in a sediment-water-mudsnail system: Enrichment, removal, metabolism and structural damage.

Novel brominated flame retardants (NBFRs) are now ubiquitous in the environment with the extensive production and application. In the present study, pentabromotoluene (PBT), hexabromobenzene (HBB) and decabromodiphenyl ethane (DBDPE) were spiked into the sediments where mudsnails (Bellamya aeruginosa) were cultivated. In the 35-day enrichment process, the highest concentration of the three NBFRs measured in mudsnail is 2.0 mg/kg, 22 mg/kg and 5.2 mg/kg dry weight (dw), respectively. The average enrichment of NBFRs in viscera was about 3 times of pleopod with the same mass. Meanwhile, the parent mudsnails can transfer NBFRs to their offspring. The removal half-life of the three NBFRs was in the range of 2.6 and 5.7 days according to the first-order kinetic equation. Several degradation products of the NBFRs were detected in mudsnail samples, which were exposed to single substance. 2,4,6-tribromotoluene was identified as degradation product of PBT; 1,2,4,5-tetrabromobenzene and 1,2,4-tribromobenzene were identified as debromination products of HBB. Possible degradation pathways were further proposed. Additionally, mudsnails after exposed to 50 mg/kg of NBFRs were observed under a scanning electron microscope, indicating that shrinkage, tissue hyperplasia and perforation occurred on the visceral surface. Such damage might be related to the accumulation of more pollutants in mudsnails viscera. As one of the few studies to explore the biological process of NBFRs, our observation could provide a scientific basis for evaluating the environmental risks of NBFRs to benthic organisms.

Interaction effects and mechanism of Pb pollution and soil microorganism in the presence of earthworm.

Heavy metals usually cause great damage to soil ecosystem. Lead (Pb) was chosen as a research object in the present study. Here repeated exposure of Pb was designed for the soil artificially contaminated. A laboratory study was conducted to determine the changes in the Pb availability and biological activity in the presence of earthworm, and the risk assessment code (RAC) was applied to evaluate the remediated soil. Results demonstrated that Pb gradually transformed to more stable fractions (OMB- and FeMnOX-Pb) under microbial action, indicating the risk level of Pb was declined. On the other hand, Pb also caused the inhibition of soil respiration and microbial biomass, and the higher the concentration of Pb, the stronger the inhibition; While in the presence of earthworm, it could absorb Pb and facilitate microbial activity, reflected the decrease of Pb content and the increase of respiration intensity in soil, as well as microbial biomass. Additionally, a good dose-response relationship between EXCH-Pb content and respiration intensity might provide a basis for ecological risk assessment.