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1.
Exp Eye Res ; 197: 108118, 2020 08.
Artículo en Inglés | MEDLINE | ID: mdl-32562694

RESUMEN

Primary angle-closure glaucoma (PACG) and retinitis pigmentosa (RP) can co-occur, but the mechanism of their association is not yet established. The purpose of this study was to investigate the differences in ocular biometry parameters and molecular genetics in patients with PACG with or without RP, and to determine the association between PACG and RP. Patients with early-onset PACG (age of onset <45 years) with or without RP were selected from the glaucoma outpatient department after full ocular examinations by the same glaucoma specialist (LX). Ocular biometry parameters were statistically analyzed. Blood samples were collected from the probands, and genomic DNA was sent out for whole exome sequencing. Variants in 326 selected genes, were extracted from the whole exome sequencing data and filtered using multiple bioinformatics analysis. The 326 genes included 10 PACG-associated genes from two genome wide association studies; 45 genes associated with anterior segment dysgenesis, microcornea, and microphthalmia; and 271 RetNet genes. Potential pathogenic variants (PPV) were obtained and underwent further genotype-phenotype analysis. As a result, a total of 32 probands with early-onset PACG were collected; nine had accompanying RP. No significant differences were noted for ocular biometry parameters between patients with PACG with RP and with PACG alone. Systematic analysis of the variants revealed that 16 of 32 probands (50%) carried PPV in 15 of 326 genes, including 14 RetNet genes and one anterior segment dysgenesis-associated gene. Of these 16 probands with PPV, five (55.56%) were from the group of nine probands with both had PACG and RP and 11 (47.83%) were from the group of 23 probands with PACG alone. Of the 15 genes, five genes, CRB1, COL2A1, RHO, RP1L1, and PAX6, were reported to cause phenotypes including glaucoma. The variants in RetNet genes appeared to be associated with a significant proportion of PACG, especially in probands with both PACG and RP. These findings enrich the phenotype spectrum of RetNet genes and provide clues for genetic screening for glaucoma. Our study suggests a genetic association between PACG and RP, although the cause-effect relationship between them needs further validation.


Asunto(s)
Predisposición Genética a la Enfermedad , Estudio de Asociación del Genoma Completo/métodos , Glaucoma de Ángulo Cerrado/genética , Mutación , Retinitis Pigmentosa/genética , Adulto , Biometría , Femenino , Humanos , Masculino , Fenotipo , Secuenciación del Exoma
2.
Ophthalmic Res ; 63(3): 244-251, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31618736

RESUMEN

PURPOSE: To assess the reproducibility of quantitative measurements of the foveal avascular zone (FAZ) and retinal vasculature determined by different scanning protocols of optical coherence tomography angiography (OCTA) in healthy volunteers. METHOD: All participants were scanned by two trained operators using an AngioPlex OCTA. Both angiography protocols (6 × 6 mm and 3 × 3 mm) were performed three times on the same eye by operator A and one additional time by operator B. The FAZ area and perimeter, retinal vessel length density (VLD) and perfusion density (PD) of different regions were analysed. RESULTS: Fifty-two eyes from 52 subjects were recruited for this study. The repeated measurements of FAZ, VLD, and PD parameters obtained by the same operator, as well as by different operators, were not significantly different when the same protocol was used (p > 0.05). The intra- and inter-operator intraclass correlation coefficients (ICCs) of the FAZ and central VLD and PD parameters (range, 0.99-0.95) were better than the intra- and inter-operator ICCs of VLD and PD in the inner and outer rings (range, 0.86-0.90). The FAZ area, perimeter, and VLDs obtained by the 3 × 3 mm protocol were larger than those obtained by the 6 × 6 mm protocol (p < 0.01), but the PDs obtained by the 3 × 3 mm protocol were smaller than those obtained by the 6 × 6 mm protocol (p < 0.001). All of the corresponding parameters obtained by the two protocols were positively correlated (r = 0.64-0.99, p < 0.001). CONCLUSION: Both the 6 × 6 and 3 × 3 mm protocols of the AngioPlex OCTA provide good reproducibility for assessing the FAZ and superficial retinal vasculature. However, the values obtained by these different protocols cannot be compared directly.


Asunto(s)
Angiografía con Fluoresceína/métodos , Mácula Lútea/irrigación sanguínea , Vasos Retinianos/diagnóstico por imagen , Tomografía de Coherencia Óptica/métodos , Adulto , Femenino , Fóvea Central/diagnóstico por imagen , Fondo de Ojo , Humanos , Mácula Lútea/diagnóstico por imagen , Masculino , Persona de Mediana Edad , Curva ROC , Valores de Referencia , Reproducibilidad de los Resultados , Vasos Retinianos/efectos de los fármacos , Adulto Joven
3.
J Glaucoma ; 2024 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-39083009

RESUMEN

PRECISE: Glaucoma patients who had previously been evaluated by eye care professionals at lower-level facilities possessed limited awareness and knowledge about their condition upon presentation to a tertiary care ophthalmic hospital, highlighting the need for improved patient education throughout the health care system. PURPOSE: To investigate the depth of knowledge about glaucoma among patients who were referred to a tertiary eye hospital for their first visit. MATERIAL AND METHODS: An internally-designed questionnaire (scored 0-15) assessing patients' knowledge about glaucoma was administered at a glaucoma outpatient service. Patients were divided into normal, high-risk, and glaucoma groups based on comprehensive eye evaluation. Scores were analyzed by regression models. The relationship between glaucoma awareness and the stage of disease at presentation was explored. RESULTS: One hundred and thirty patients were enrolled and divided into three groups. The group with definitive diagnosis of glaucoma had the most prior medical visits but scored the lowest, with the primary source of information being previous health care providers. The high-risk group possessed more knowledge about glaucoma than the other groups and tended to acquire knowledge from the media and sought tertiary care earlier. Significant differences were observed between the glaucoma and the high-risk groups in all aspects of glaucoma knowledge (P < 0.05). Additionally, the average scores of all participants who had visited lower-level facilities were low. Education, economic status, presence of risk factors for glaucoma positively correlated with awareness and knowledge, whereas age had an inverse relationship (all P < 0.05). Ophthalmic visit frequency had no impact (P > 0.05). Doctors were the primary source of information for all groups, but social media users were better informed. CONCLUSIONS: Patients at tertiary eye care centers lack glaucoma knowledge, despite experience with eye care providers previously. Implementing health education at all levels is crucial in preventing glaucoma-related visual impairment.

4.
Front Med (Lausanne) ; 9: 828991, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35391877

RESUMEN

Purpose: This study aimed to investigate the differences in longitudinal changes in the peripapillary retinal nerve fiber layer (pRNFL) and macular ganglion cell plus inner plexiform layer (GCIPL) caused by progressive myopia and glaucoma among adolescents. Design: This was a retrospective observational study. Methods: A total of forty-seven and 25 eyes of 47 and 25 adolescents with myopia progression (MP) and glaucoma progression (GP), respectively, who were followed up at the Zhongshan Ophthalmic Center for at least 3 years, were included in the study. The pRNFL and GCIPL that measured at the initial and last visits were analyzed. Results: The median follow-up period was 5 years for both two groups. During follow-up, the whole, superior, and inferior pRNFL decreased in both the MP and GP groups, (p < 0.001). Nasal pRNFL decreased in the MP group (p < 0.001) but had no significant difference in the GP group (p = 0.19). Temporal pRNFL was increased in the MP group (p < 0.001) but decreased in the GP group (p < 0.001). The average and sectoral GCIPL decreased in both groups (p < 0.001). The annual change rate of temporal pRNFL and pRNFL at 10-, 8-, 9-, and 7-clock-hour sectors and the inferotemporal GCIPL has better diagnostic value to differentiate glaucoma from myopia (the area under the receiver operating characteristic curve, AUC > 0.85). Conclusion: Glaucoma and MP could cause loss of the pRNFL and GCIPL in adolescents; however, the loss patterns were different between the two groups. The temporal quadrant and 7-, 8-, 9-, and 10-clock-hour sector pRNFL and the inferotemporal GCIPL can help distinguish pRNFL and GCIPL loss caused by glaucoma or MP.

5.
Front Med (Lausanne) ; 8: 772578, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34805232

RESUMEN

Background: The purpose of this study was to analyze the ocular biometric parameters of primary angle-closure disease (PACD) in younger patients and compare them with those of elderly patients. Methods: This clinic-based, cross-sectional study included 154 eyes of 154 patients with PACD, consisting of 77 eyes of patients aged 40 years or younger and 77 eyes of patients older than 40. The PACD case definition was compatible with the ISGEO definition. Anterior segment parameters were measured by ultrasound biomicroscopy, axial length (AL) and lens thickness (LT) were measured by A-scan ultrasonography measurements, and the thickness of the retina and choroid were measured by optical coherence tomography. The differences in ocular biometric parameters between different age groups were compared by independent sample t-tests or Mann-Whitney U tests, and the correlation between the parameters and age was analyzed. Results: Compared to older PACD patients, the lens vault(LV),LV/LT and subfoveal choroidal thickness (SFCT) of younger patients were larger, while the peripheral and mean iris thickness (IT), trabecular-ciliary angle (TCA), ciliary body thickness (CBT), AL and LT were smaller (all P < 0.01). There was no significant difference in anterior chamber depth, anterior chamber width, pupil diameter, angle opening distance at 500 µm from the scleral spur, anterior chamber angle and iris convexity between the two groups (all P > 0.05). AL, LT, IT, TCA and CBT were positively associated with age (all P < 0.001), while LV and SFCT were negatively associated with age (P = 0.027 and P < 0.001, respectively). Conclusions: Compared with elderly patients, younger PACD patients had more anteriorly positioned lenses, thinner and more anteriorly rotated ciliary bodies, thicker choroids, and shorter axial length. These characteristics might be important anatomical bases for the earlier onset of PACD and the higher risk of malignant glaucoma after filtering surgery.

6.
Zhonghua Yan Ke Za Zhi ; 45(7): 621-4, 2009 Jul.
Artículo en Zh | MEDLINE | ID: mdl-19957690

RESUMEN

OBJECTIVE: To investigate the effect of gene screening on forewarning and monitoring of familial open-angle glaucoma pedigree. METHODS: Comprehensive ophthalmic examinations were performed in all available family members. The genomic DNA was extracted from peripheral blood. The myocilin gene was amplified and screened for mutations using direct sequencing. All family members were followed up. RESULTS: Among 12 family members, 5 individuals carry a C to T transition in exon 3 resulting in the substitution of proline to leucine (Pro370Leu), and the other 7 individuals did not carry this mutation. Ophthalmic examinations did not show any abnormality in the optic disc, the thickness of RNFL, and visual field parameters in mutation-carriers. During the follow-up, all carriers were diagnosed as open-angle glaucoma. The mean time of presentation of the defect of visual field was 21.6 months and 14.4 months after the changes in RNFL thickness. CONCLUSION: Genetic diagnosis was proven to be a method with high specificity and sensitivity; and can be used for presymptom diagnosis and forewarning in familial open-angle glaucoma pedigree.


Asunto(s)
Proteínas del Citoesqueleto/genética , Proteínas del Ojo/genética , Glaucoma de Ángulo Abierto/diagnóstico , Glaucoma de Ángulo Abierto/genética , Glicoproteínas/genética , Mutación , Adolescente , Niño , ADN , Femenino , Pruebas Genéticas , Humanos , Masculino , Linaje , Sensibilidad y Especificidad
7.
Zhonghua Yan Ke Za Zhi ; 45(4): 338-43, 2009 Apr.
Artículo en Zh | MEDLINE | ID: mdl-19575967

RESUMEN

OBJECTIVE: To evaluate the effect of trabeculectomy on intraocular pressure (IOP) and complications in eyes with primary angle closure glaucoma (PACG). METHODS: It was retrospective clinical study. PACG was classified as acute primary angle closure glaucoma (APACG) group and chronic primary angle closure glaucoma (CPACG) group. APACG was then divided into acute attack phase and chronic phase, and CPACG was divided into chronic phase and late phase. IOP, best corrected visual acuity were compared before and after trabeculectomy in different subgroup of PACG. In addition, the incidence of complications of trabeculectomy was assessed. Statistical analyses were performed using SPSS 12.0 statistics software. Categorical variables such as best corrected visual acuity were compared using nonparametric test, continuous variables such as age and IOP were compared between the two groups using independent two-sample t-tests. Pre- and postoperative IOP were compared using one-way analysis of variance of repeated measures. RESULTS: 40 eyes (37 cases) of APACG and 56 eyes (45 cases) of CPACG were enrolled in this study. The median follow-up period was 24 and 25 months, respectively. IOP was significantly decreased from (53.6 +/- 17.9) mm Hg (1 mm Hg = 0.133 kPa, preoperation) to (10.5 +/- 4.9) mm Hg (postoperation) at time of discharging from hospital and (14.0 +/- 10.3) mm Hg at time of final follow up in APACG (F = 100.783, P < 0.01), respectively, and from (36.8 +/- 13.8) mm Hg to (11.7 +/- 4.2) mm Hg at time of discharging from hospital and (13.8 +/- 4.5) mm Hg at time of final follow up in CPACG (F = 54.383, P < 0.01), respectively. The IOP remained controlled (< or = 21 mm Hg) without antiglaucomatous medication in 38 eyes (95.0%) of APACG and in 50 eyes (89.3%) of CPACG. Visual acuity was significantly (H = 12.316, P < 0.01) decreased after trabeculectomy in all sub-types of PACG by Kruskal-wallis analysis. Shallow anterior chamber was commonly occurred after trabeculectomy. CONCLUSIONS: Trabeculectomy is an effective method to control IOP in APACG and CPACG. However, the high proportion of impaired vision was found following trabeculectomy in this study and warranted further clinical investigation.


Asunto(s)
Glaucoma de Ángulo Cerrado/cirugía , Trabeculectomía/métodos , Adulto , Anciano , Femenino , Glaucoma de Ángulo Cerrado/fisiopatología , Humanos , Presión Intraocular , Masculino , Persona de Mediana Edad , Estudios Retrospectivos
8.
Mol Med Rep ; 19(1): 468-476, 2019 01.
Artículo en Inglés | MEDLINE | ID: mdl-30483803

RESUMEN

Subconjunctival fibrosis represents the primary cause of postoperative failure of trabeculectomy, and at present there is a lack of effective intervention strategies. The present study aimed to investigate the effect of the mitogen­activated protein kinase kinase (MEK) inhibitor U0126 on human tenon fibroblast (HTF) myofibrosis transdifferentiation, and to illuminate the underlying molecular mechanisms involved. It was demonstrated that U0126 significantly inhibited the proliferation, migration and collagen contraction of HTFs stimulated with TGF­ß1. In addition, U0126 largely attenuated the TGF­ß1­induced conversion of HTFs into myofibroblasts, as indicated by a downregulation of the mRNA and protein expression of α­smooth muscle actin and zinc finger protein SNAI1, and by ameliorating the 3D­collagen contraction response. Mechanistically, U0126 suppressed the TGF­ß1­stimulated phosphorylation of mothers against decapentaplegic homolog 2/3, P38 mitogen­activated protein kinase and extracellular signal­regulated kinase 1/2, indicating that U0126 may inhibit HTF activation through the canonical and non­canonical signaling pathways of TGF­ß1. Therefore, U0126 exhibits a potent anti­fibrotic effect among HTFs, and the inhibition of MEK signaling may serve as an alternative intervention strategy for the treatment of trabeculectomy­associated fibrosis.


Asunto(s)
Transdiferenciación Celular/fisiología , Fibroblastos/metabolismo , Proteínas Quinasas Activadas por Mitógenos/antagonistas & inhibidores , Miofibroblastos/metabolismo , Factor de Crecimiento Transformador beta1/metabolismo , Actinas/metabolismo , Butadienos/farmacología , Diferenciación Celular/fisiología , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Transdiferenciación Celular/efectos de los fármacos , Células Cultivadas , Colágeno/metabolismo , Regulación hacia Abajo/efectos de los fármacos , Fibroblastos/efectos de los fármacos , Fibrosis/tratamiento farmacológico , Fibrosis/metabolismo , Humanos , Miofibroblastos/efectos de los fármacos , Nitrilos/farmacología , Fosforilación/efectos de los fármacos , ARN Mensajero/metabolismo , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Factores de Transcripción de la Familia Snail/metabolismo
9.
Zhonghua Yan Ke Za Zhi ; 42(3): 199-203, 2006 Mar.
Artículo en Zh | MEDLINE | ID: mdl-16643748

RESUMEN

OBJECTIVE: To evaluate central corneal thickness (CCT) of normal subjects, patients with primary open angle glaucoma (POAG), normal tension glaucoma (NTG) and ocular hypertension (OHT) using optical coherence tomography (OCT). METHODS: One hundred and forty-three eyes of 143 normal subjects, 36 eyes of 36 patients with POAG, 39 eyes of 39 patients with NTG and 40 eyes of 40 patients with OHT were enrolled. CCT were measured by OCT3 and analyzed using ANOVA. The relationship of CCT and intraocular pressure (IOP) measured by Goldmann applanation tonometry were analyzed by linear regression. The inter-observer and intra-observer agreement of CCT measurement by OCT were analyzed by interclass correlation coefficient (ICC). RESULTS: The average CCT of normal subjects, patients with POAG, NTG and OHT were (525.31 +/- 32.18) microm, (531.87 +/- 31.58) microm, (507.61 +/- 21.56) microm and (574.09 +/- 27.84) microm, respectively. There was no significant difference between normal subjects and patients with POAG (P = 0.099). The average CCT of OHT patients was larger than those of other groups (P < 0.001). The average CCT of patients with NTG was less than those of other groups (P < 0.001). There was positive relationship between CCT and IOP in normal subjects (r = 0.318, R2 = 0.101, P < 0.001). The ICC of inter-observer and intra-observer agreement of CCT measurement by OCT were 0.995 and 0.996 respectively. CONCLUSIONS: Central corneal thickness could be measured by OCT accurately. The average CCT in the patients with NTG was thinner, but the average CCT in the patients with OHT was thicker than that of normal subjects. CCT could affect IOP measurement in the application of Goldmann applanation tonometry but with little significant.


Asunto(s)
Córnea/patología , Glaucoma de Ángulo Abierto/patología , Tomografía de Coherencia Óptica , Adolescente , Adulto , Anciano , Córnea/anatomía & histología , Femenino , Glaucoma de Ángulo Abierto/fisiopatología , Humanos , Presión Intraocular , Masculino , Persona de Mediana Edad , Hipertensión Ocular/patología , Hipertensión Ocular/fisiopatología , Tonometría Ocular
10.
Zhonghua Yan Ke Za Zhi ; 42(3): 209-11, 2006 Mar.
Artículo en Zh | MEDLINE | ID: mdl-16643750

RESUMEN

OBJECTIVE: To study the effect of dexamethasone on trans epithelial electrical resistance (TEER) and the expression of AQP-1 in immortalized trabecular cells. METHODS: Immortalized human trabecular cells were cultured on PET membrane in 24 wells plate. The cells were treated with Dexamethasone in concentration of 10(-7) mol/L. After the cultured cell became confluence, TEER of the cells was detected to evaluate the resistance of outflow pathway at different time points. Western blot was used to analyze the expression of AQP-1 in the trabecular cells. RESULTS: The TEER and the expression of AQP-1 were much higher in the cells treated by Dexamethasone in comparison to control without dexamethasone exposure. CONCLUSIONS: The TEER and expression of AQP-1 in immortalized trabecular cells can be enhanced by Dexamethasone. AQP-1 upregulation induced by Glucocorticoid may relate with the increased resistance of outflow pathway in glaucoma.


Asunto(s)
Acuaporina 1/biosíntesis , Dexametasona/farmacología , Malla Trabecular/efectos de los fármacos , Línea Celular , Impedancia Eléctrica , Humanos , Malla Trabecular/fisiopatología
11.
Zhonghua Yan Ke Za Zhi ; 41(12): 1068-75, 2005 Dec.
Artículo en Zh | MEDLINE | ID: mdl-16409756

RESUMEN

OBJECTIVE: To determine the causative mutation of myocilin gene and to investigate its pathogenic function in a large Chinese pedigree (GZ.1) with familial open-angle glaucoma. METHODS: Genome-wide scanning was performed and the Lod scores were calculated. Candidate gene was amplified and screened for mutations using direct sequencing. To elucidate its expression, distribution and cytotoxicity of mutant myocilin, human trabecular cells (HTM) cells were transfected with pcDNA-wild-type and mutant myocilin vectors using liposomes. RESULTS: Mutation analysis of the myocilin gene showed a C-to-T transition at the 1, 109 th nucleotide in exon 3 resulting in a change of amino acid from proline to leucine (Pro370Leu). This mutation cosegregated with all affected individuals (16/16) and never presented in unaffected individuals (0/8). In transfected HTM cells, the mutant myocilin protein was not correctly processed in ER and accumulated as aggresome-like structures in the cytoplasma instead of being secreted. In addition, the expression of mutant protein also led to apoptosis of trabecular cells and the occurrence of. CONCLUSION: The mutation of Pro370Leu in myocilin gene could cause the accumulation of misfolding myocilin protein in HTM cells, which might lead to glaucoma in GZ.1 pedigree.


Asunto(s)
Proteínas del Citoesqueleto/genética , Proteínas del Ojo/genética , Glaucoma de Ángulo Abierto/genética , Glicoproteínas/genética , Mutación , Malla Trabecular/metabolismo , Pueblo Asiatico/genética , Ligamiento Genético , Humanos , Linaje , Pliegue de Proteína , Malla Trabecular/patología
12.
PLoS One ; 10(11): e0142858, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26575284

RESUMEN

PURPOSE: To study willingness to pay for cataract surgery and surgical service provided by a senior cataract surgeon in urban Southern China. METHODS: This study was a cross-sectional willingness-to-pay (WTP) interview using bidding formats. Two-hundred eleven persons with presenting visual impairment in either eye due to cataract were enrolled at a tertiary eye hospital. Participants underwent a comprehensive eye examination and a WTP interview for both surgery and service provided by a senior surgeon. Demographic information, socioeconomic status and clinical data were recorded. RESULTS: Among 211 (98% response rate) persons completing the interview, 53.6% were women and 80.6% were retired. About 72.2% had a monthly income lower than 1000 renminbi (US $161). A total of 189 (89.6%) were willing to pay for cataract and the median amount of WTP was 6000 renminbi (US$968). And 102 (50.7%) were willing to pay additional fees for surgery performed by a senior surgeon, and the median amount of WTP was 500 renminbi (US$81). In regression models adjusting for age and gender, persons with preexisting eye diseases other than cataract, were more likely to pay for cataract surgery and service provided by a senior surgeon (P = 0.04 for both). CONCLUSIONS: In urban China, cataract patients, especially those with preexisting eye conditions, are willing to pay additional fees for a senior surgeon. Moving to a system where the price of cataract surgery is proportional to the consultant' skill and expertise is possible and may have a potential impact on waiting list and quality of eye care. Further studies are needed to examine the impact of such pricing system on attitudes and choices of cataract patients.


Asunto(s)
Extracción de Catarata , Catarata/psicología , Anciano , Catarata/economía , China , Estudios Transversales , Demografía , Femenino , Humanos , Entrevistas como Asunto , Masculino , Análisis Multivariante , Análisis de Regresión , Cirujanos/clasificación , Encuestas y Cuestionarios , Centros de Atención Terciaria
13.
Chin Med J (Engl) ; 116(3): 444-7, 2003 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-12781056

RESUMEN

OBJECTIVE: To identify the characteristics of and evaluate surgical impact on idiopathic macular hole (IMH) by using an optical coherence tomography (OCT) scanner. METHODS: Sixty-five cases (70 eyes) experiencing IMH were examined using OCT, then graded by their clinical characteristics. Nineteen cases (19 eyes) were scanned and measured using OCT before and after surgery. RESULTS: Of the 70 eyes,the number of stage I-IV macular holes were 11, 12, 36 and 11, respectively. For stage I holes, the OCT images revealed flattened or nonexistent fovea and minimally reflective space within or beneath the neurosensory retina; stage II holes appeared to be full-sized with attached operculum and surrounding edema; stage III holes were also full-sized with surrounding edema; finally, stage IV holes were full-sized and completely separated the posterior hyaloid membrane from the retina. Through quantitative measurements, OCT determined that the values for mean hole diameter, mean halo diameter and mean thickness of the hole's edge were reduced from 570.95 +/- 265.59 to 337.05 +/- 335.95 microm, 1043.53 +/- 278.8 to 695.00 +/- 483.00 microm and 389.78 +/- 60.58 to 298.78 +/- 109.80 microm, respectively in 19 IMH cases after surgery. In 17 eyes, the holes or halos eventually closed or were reduced in size, or the edges of the holes thinned out. The anatomic successful rate of the surgery was 89%. CONCLUSION: OCT can exhibit the characteristics of IMH and measure the diameter of holes quantitatively. This method can also judge the surgical impacts of IMH objectively, accurately and effectively.


Asunto(s)
Mácula Lútea/patología , Perforaciones de la Retina/patología , Tomografía/métodos , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Perforaciones de la Retina/cirugía
14.
Zhonghua Yan Ke Za Zhi ; 40(10): 683-8, 2004 Oct.
Artículo en Zh | MEDLINE | ID: mdl-16200859

RESUMEN

OBJECTIVE: To study the optic nerve head (ONH) parameters and topograph features of normal subjects, and analyze the effect of gender, laterality, age and disc size on the topograph of ONH. METHODS: Heidelberg Retina Tomograph (HRT) was used to obtain the optic nerve head parameters and topograph images in 216 eyes of 116 normal volunteers. The impact of gender, laterality, age and disc size on the topograph was analyzed. The optic disc was divided into three groups by their size, small (less than 1.5 mm2), middle and large disc (equal or larger than 2.5 mm2), to evaluate the differences of parameters. RESULTS: The parameters of ONH in normal eyes were as following: disc area (DA) (2.208 +/- 0.411) mm, cup area (CA) (0.466 +/- 0.355) mm2, cup/disk area ratio (C/DAR) (0.217 +/- 0.137), rim area (RA) (1.563 +/- 0.339) mm2, height variation contour (HVC) (0.404 +/- 0.096) mm, cup volume (CV) 0.127 mm3, rim volume (RV) (0.446 +/- 0.173) mm3, mean cup depth (MCD) (0.196 +/- 0.096) mm, maximum cup depth (MxCD) (0.559 +/- 0.228) mm, cup shape measure (CSM) (-0.213 +/- 0.076), mean retinal nerve fiber layer thickness (mRNFLT) (0.272 +/- 0.076) mm, and retinal nerve fiber layer cross section area (RNFLA) (1.364 +/- 0.375) mm2. There were significant differences between right and left eyes in C/DAR, RA and RV, but no difference between different genders in all parameters. A negative correlation was found between mRNFLT, RNFLA and age. There were significant differences in most parameters among three groups with different disc size, such as CA, C/DAR, CV, RA and CSM, but not in mRNFLT, RNFLA and RV. CONCLUSIONS: Significant variations were found in parameters of ONH in normal subjects. Some parameters of ONH were affected by age and optic disc size. There were differences in some parameters between right and left eyes, but not between different genders. There were differences of parameters between different quadrants of ONH.


Asunto(s)
Disco Óptico/anatomía & histología , Tomografía/normas , Adolescente , Adulto , Factores de Edad , Anciano , Niño , Preescolar , Técnicas de Diagnóstico Oftalmológico/normas , Femenino , Humanos , Masculino , Persona de Mediana Edad , Valores de Referencia , Retina/anatomía & histología , Factores Sexuales
15.
Zhonghua Yan Ke Za Zhi ; 40(11): 737-40, 2004 Nov.
Artículo en Zh | MEDLINE | ID: mdl-15634479

RESUMEN

OBJECTIVE: To evaluate the ability of Heidelberg retina tomography (HRT) in the diagnosis of primary open angle glaucoma (POAG). METHODS: The topography of optic nerve head of 116 normal eyes and 99 eyes with POAG were examined using HRT. The differences of the optic disc parameters between two groups were compared. The topographic parameters of the optic nerve head were undergone stepwised discriminant analysis and the discriminant function was established. The sensitive diagnostic parameters of POAG were screened. RESULTS: The topographic parameters of optic nerve head, including disc area (DA), cup area (CA), cup/disc area ratio (C/DAR), rim area (RA), height variation contour (HVC), cup volume (CV), rim volume (RV), mean cup depth (MCD), maximum cup depth (MxCD), cup shape measure (CSM), mean retinal nerve fiber layer thickness (mRNFLT), retinal nerve fiber layer cross section area (RNFLA), showed significant (P < 0.001) difference between normal subjects and patients with POAG. The sensitive diagnostic parameters of POAG were C/DAR, RA, CSM, CV, MCD. The discriminant function was Y = -2.083-4.833 x C/DAR-20.379 x CSM + 2.035 x RA + 3.955 x CV-3.701 x MxCD. The sensitivity, specificity and diagnostic accuracy were 81.8%, 86.2% and 84.2%, respectively, which were comparable to the automatic analysis program of HRT. CONCLUSIONS: The topographic parameters of optic nerve head in patients with POAG showed significant differences from that in normal people. The power of discriminant function established based on our data was comparable to the HRT automatic analysis program. Our results indicate that HRT automatic analysis program is applicable to Chinese.


Asunto(s)
Glaucoma de Ángulo Abierto/diagnóstico , Enfermedades del Nervio Óptico/diagnóstico , Tomografía/normas , Adolescente , Adulto , Anciano , Pueblo Asiatico , Niño , Preescolar , China , Técnicas de Diagnóstico Oftalmológico/normas , Femenino , Glaucoma de Ángulo Abierto/complicaciones , Humanos , Masculino , Persona de Mediana Edad , Disco Óptico/patología , Enfermedades del Nervio Óptico/etiología , Valores de Referencia
16.
Zhonghua Yan Ke Za Zhi ; 38(5): 265-7, 2002 May.
Artículo en Zh | MEDLINE | ID: mdl-12133369

RESUMEN

OBJECTIVE: To investigate the effects of phacoemulsification on the macula following uncomplicated phacoemulsification by optical coherence tomography (OCT). METHODS: Eighty eyes of the senile cataract were chosen randomly. The uncomplicated phacoemulsification was performed. OCT was examined preoperatively and 1 week after the surgery. Preoperative visual acuity, the retinal thickness and phaco power were compared with those after surgery. RESULTS: In 80 eyes, the preoperative mean foveal thickness was (142.9 +/- 16.7) micrometer and the postoperative (157.9 +/- 36.7) micrometer, the difference being not significant (P > 0.05). Three eyes had macular edema 1 week after surgery. In 11 eyes with Tyndall sign (+ +), the mean postoperative foveal thickness was thicker than the mean preoperative value (P < 0.05). In lower phaco power group, the mean postoperative foveal thickness was (156.2 +/- 18.3) micrometer and the higher phaco power group was (172.6 +/- 32.9) microm (P < 0.05). The best corrected visual acuity after surgery had a negative correlation with the retinal thickness. CONCLUSIONS: The retinal thickening and macular edema can be found after uncomplicated phacoemulsification. The higher phaco power results in significant inflammation and thicker retina. The visual consequences were proportional to the degrees of macular thickening.


Asunto(s)
Mácula Lútea/patología , Edema Macular/patología , Facoemulsificación/efectos adversos , Complicaciones Posoperatorias/patología , Enfermedades de la Retina/patología , Tomografía/métodos , Anciano , Catarata/terapia , Femenino , Humanos , Edema Macular/diagnóstico , Masculino , Persona de Mediana Edad , Facoemulsificación/métodos , Complicaciones Posoperatorias/diagnóstico , Retina/patología
17.
Zhonghua Yan Ke Za Zhi ; 39(8): 471-5, 2003 Aug.
Artículo en Zh | MEDLINE | ID: mdl-14642167

RESUMEN

OBJECTIVE: To evaluate the interobserver and intraobserver agreement of parameters of optic nerve head by Heidelberg Retina Tomograph (HRT). METHODS: Optic nerve heads from 50 eyes (30 subjects) were imaged with the HRT. Three ophthalmologists majoring in glaucoma traced the disc margin with a contour line. One of these observers, repeatedly traced the disc margin with contour line in triplicate at three different times. Eleven topographic parameters of optic nerve head (including disc area, cup area, cup/disc area ratio, rim area, cup volume, rim volume, mean cup depth, maximum cup depth, cup shape measure, mean retinal nerve fiber layer thickness, sectional area of retinal nerve fiber layer) were analyzed with HRT software (version 2.01). Intraclass coefficients (ICC) and agreement of result estimation (kappa value) were calculated. RESULTS: The ICCs results among three observers on 12 parameters of optic nerve head topography were 0.976 - 0.999, which showed almost perfect agreement; and the kappa value were 0.649 approximately 0.767, which showed substantial agreement. The ICC of the same observer in different times were 0.983 approximately 0.999, which showed almost perfect agreement; and the Kappa values were 0.810 approximately 0.951, which showed substantial agreement. CONCLUSIONS: Tracing the optic nerve head with contour line on HRT image is substantial to almost perfect within both interobserver and intraobserver in experienced ophthalmologist. This procedure is suitable for clinical diagnosis and follow-up observation of glaucoma.


Asunto(s)
Glaucoma de Ángulo Abierto/diagnóstico , Disco Óptico/patología , Tomografía/estadística & datos numéricos , Adolescente , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Valores de Referencia , Retinoscopía/métodos
18.
Cell Stress Chaperones ; 19(6): 827-35, 2014 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24643796

RESUMEN

Endoplasmic reticulum (ER) stress has been implicated in various neurodegenerative diseases, including Alzheimer's disease. We have previously observed amyloid production in the retina of the Tg2576 transgenic mouse model of Alzheimer's disease. In this study, we used tunicamycin-induced ER stress in RGC-5 cells, a cell line identical to the photoreceptor cell line 661W, to investigate the effect of ER stress on production of amyloid-beta (Abeta) peptides. We found that the mRNA level of amyloid-beta precursor protein (APP) remained stable, while the protein level of amyloid-beta precursor protein (APP) was decreased, the amyloid-beta precursor protein cleaving enzymes beta-site APP-cleaving enzyme 1 and presenilin 1 were upregulated, Abeta1-40 and Abeta1-42 production were increased, and reactive oxygen species production and apoptosis markers were elevated following induction of ER stress. The protein level of Abeta degradation enzymes, neprilysin, endothelin-converting enzyme 1, and endothelin-converting enzyme 2 remained unchanged during the prolonged ER stress, showing that the generation of Abeta did not result from reduction of proteolysis by these enzymes. Inclusion of group II caspase inhibitor, Z-DEVD-FMK, increased the ER stress mediated Abeta production, suggesting that they are generated by a caspase-independent mechanism. Our findings provided evidence of a role of ER stress in Abeta peptide overproduction and apoptotic pathway activation in RGC-5 cells.


Asunto(s)
Péptidos beta-Amiloides/metabolismo , Estrés del Retículo Endoplásmico , Retículo Endoplásmico/metabolismo , Células Ganglionares de la Retina/metabolismo , Secretasas de la Proteína Precursora del Amiloide/metabolismo , Péptidos beta-Amiloides/genética , Animales , Apoptosis , Ácido Aspártico Endopeptidasas/metabolismo , Caspasa 3/metabolismo , Línea Celular , Retículo Endoplásmico/efectos de los fármacos , Retículo Endoplásmico/patología , Estrés Oxidativo , Fragmentos de Péptidos/metabolismo , Presenilina-1/metabolismo , Estabilidad del ARN , ARN Mensajero/metabolismo , Ratas , Especies Reactivas de Oxígeno/metabolismo , Células Ganglionares de la Retina/efectos de los fármacos , Células Ganglionares de la Retina/patología , Transducción de Señal , Factores de Tiempo , Factor de Transcripción CHOP/metabolismo , Tunicamicina/farmacología , Regulación hacia Arriba
19.
Eye Sci ; 28(3): 113-8, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24579550

RESUMEN

PURPOSE: Nanophthalmos is a rare congenital disorder associated with a high incidence of angle-closure glaucoma. We followed the clinical manifestations of three siblings to evaluate their responses to various treatments. METHODS: Three sisters with nanophthalmos were followed from 2000 to 2013. Glaucoma and cataract treatments were performed whenever indicated. RESULTS: The oldest sister had chronic elevation of intraocular pressure (IOP) and underwent laser peripheral iridotomy (LPI) on both eyes, followed by uneventful phacoemulsification with intraocular lens (IOL) implantation on the left eye and phacotrabeculectomy with IOL implantation on the right eye. The middle sister had acute elevation of IOP and initially underwent phacoemulsification combined with implantation of two IOLs on her left eye and LPI on her right eye. Severe uveal effusion occurred when phacoemulsification was performed on her right eye 6 years later, but ultimately was completely resolved. In both sisters, stable IOP and visual results were achieved after lensectomy. The youngest sister, who had suspected angle-closure, achieved a stable IOP and visual results with prophylactic LPI alone. CONCLUSION: In nanophthalmic eyes, the severity of the disease may foreshadow the severity of surgical complications and responses to therapy.


Asunto(s)
Glaucoma de Ángulo Cerrado/terapia , Implantación de Lentes Intraoculares , Microftalmía/complicaciones , Hipertensión Ocular/cirugía , Facoemulsificación , Hermanos , Femenino , Estudios de Seguimiento , Humanos , Presión Intraocular , Iris/cirugía , Terapia por Láser/métodos , Hipertensión Ocular/complicaciones , Facoemulsificación/efectos adversos , Facoemulsificación/métodos , Factores de Tiempo , Trabeculectomía/métodos , Resultado del Tratamiento , Agudeza Visual
20.
Chin Med J (Engl) ; 125(8): 1429-33, 2012 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-22613648

RESUMEN

BACKGROUND: Trabeculectomy has become a mainstream treatment in intraocular pressure (IOP) reduction for primary angle-closure glaucoma (PACG); combined trabeculectomy and cataract surgery was reported to reduce IOP and simultaneously improve vision for patients with PACG and coexisting cataract. This study was specialized to compare the efficacy and safety of combined phacotrabeculectomy with that of trabeculectomy only in the treatment of PACG with coexisting cataract. METHODS: This is a comparative case series study. Thirty-one patients (31 eyes) with PACG and coexisting cataract were enrolled. Of these, 17 underwent phacotrabeculectomy and 14 underwent trabeculectomy alone. IOP, filtering blebs, and complications were compared at the final follow-up. Complete success was defined as a final IOP less than 21 mmHg without IOP-lowering medication. RESULTS: After 10 months of postoperative follow-up, the phacotrabeculectomy and trabeculectomy groups showed no significant differences regarding IOP reduction ((20.59 ± 7.94) vs. (24.85 ± 14.39) mmHg, P = 0.614), complete success rate (88% vs. 71%, P = 0.370), formation rate of functioning blebs (65% (11/17) vs. 93% (13/14), P = 0.094), and complications (41% (7/17) vs. 57% (8/14), P = 0.380). IOP-lowering medication was not required for most of the patients in both groups. Additional surgery interventions, including anterior chamber reformation and phacoemulsification, were needed in the trabeculectomy group, whereas no surgery was needed postoperatively in the phacotrabeculectomy group. CONCLUSION: Phacotrabeculectomy and trabeculectomy treatments exhibit similar IOP reduction, successful rates, and complications when it comes to treating PACG patients with coexisting cataract, although additional surgery intervention may be needed for a few cases with cataract and complications after trabeculectomy.


Asunto(s)
Glaucoma de Ángulo Cerrado/cirugía , Facoemulsificación/métodos , Trabeculectomía/métodos , Anciano , Extracción de Catarata , Femenino , Glaucoma de Ángulo Cerrado/fisiopatología , Humanos , Presión Intraocular , Masculino , Persona de Mediana Edad , Facoemulsificación/efectos adversos , Complicaciones Posoperatorias/etiología , Trabeculectomía/efectos adversos , Agudeza Visual
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