Comparison of different primers for rapid detection of Vibrio parahaemolyticus using the polymerase chain reaction.

AIMS: The aim of this study was to compare different primers for rapid and effective detection of Vibrio parahaemolyticus by polymerase chain reaction (PCR). METHODS AND RESULTS: A total of four pairs of primers, three previously published and one based on a newly developed V. parahaemolyticus metalloprotease (vpm) gene, have been assayed for PCR detection of V. parahaemolyticus. They have been tested for specificity and sensitivity on a total of 101 strains including reference and environment isolates belonging to V. parahaemolyticus and other species in Vibrio. Of the four sets of primers tested, the one designed on the basis of the metalloprotease gene (675 bp) gave optimal results with bacterial strains examined as they only amplified the specific fragment in strains that had been genetically and biochemically assessed as V. parahaemolyticus and the limit of detection was 4 pg of purified target DNA. CONCLUSIONS: The primers designed on the metalloprotease gene gave optimal results for specific, sensitive and rapid detection of V. parahaemolyticus by PCR. SIGNIFICANCE AND IMPACT OF THE STUDY: PCR amplification with the optimal primer set VPM1/VPM2 could facilitate the rapid diagnosis and surveillance of potentially pathogenic strains of V. parahaemolyticus and reduce food-borne illness.

[Laryngeal function preservation in comprehensive therapy of carcinoma of pyriform sinus].

OBJECTIVE: To investigate the sufficiency of comprehensive therapy in the preservation of laryngeal function of carcinoma of pyriform sinus. METHOD: Since 1986, 33 cases of pyriform sinus carcinoma were treated, the clinical stages were as follows: stage I 2 cases, stage II 9 cases, stage III 12, stage IV 10. The induced chemotherapy agents were cisplatin, methotrexate and pingyangmycin. Surgical treatment include local dissection of tumor, partial hypopharynx and partial larynx dissection. All patients were received postoperative radiotherapy. RESULT: Chemotherapy partial response rate was 42.4%, 3 years survive rate was 54.8%, 5 years survive rate was 38.7%. CONCLUSION: The cisplatin centered combined induced chemotherapy was effective in the treatment of pyriform sinus, the laryngeal function preservation surgical treatment of pyriform sinus carcinoma in T1 T2 and selective T3 patients is practicable. Postoperative radiotherapy can improve the local control rate.

[The treatment of hypopharyngoesophageal stenosis].

OBJECTIVE: To improve the curative effects of hypopharyngoesophageal stenosis. METHOD: 35 patients treated for hypopharyngoesophageal stenosis were retrospectively reviewed. The curative methods were selected on the stage of stenosis, including drug, dilatation, reconstruction of hypopharynx and oesophagus. RESULT: 33 patients resumed a normal oral diet, no stenosis of anastomosis occurred. CONCLUSION: For the early mild lesions, using of steroids, antibacterial and dilatation is considered to be the procedure of choice, for those severe lesions, reconstruction of hypopharynx and oesophagus with selected appropriate materials can obtain good results.