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1.
Glycobiology ; 34(6)2024 Apr 24.
Artículo en Inglés | MEDLINE | ID: mdl-38760939

RESUMEN

Genetic deficiency of alpha-L-iduronidase causes mucopolysaccharidosis type I (MPS-I) disease, due to accumulation of glycosaminoglycans (GAGs) including chondroitin/dermatan sulfate (CS/DS) and heparan sulfate (HS) in cells. Currently, patients are treated by infusion of recombinant iduronidase or by hematopoietic stem cell transplantation. An alternative approach is to reduce the L-iduronidase substrate, through limiting the biosynthesis of iduronic acid. Our earlier study demonstrated that ebselen attenuated GAGs accumulation in MPS-I cells, through inhibiting iduronic acid producing enzymes. However, ebselen has multiple pharmacological effects, which prevents its application for MPS-I. Thus, we continued the study by looking for novel inhibitors of dermatan sulfate epimerase 1 (DS-epi1), the main responsible enzyme for production of iduronic acid in CS/DS chains. Based on virtual screening of chemicals towards chondroitinase AC, we constructed a library with 1,064 compounds that were tested for DS-epi1 inhibition. Seventeen compounds were identified to be able to inhibit 27%-86% of DS-epi1 activity at 10 µM. Two compounds were selected for further investigation based on the structure properties. The results show that both inhibitors had a comparable level in inhibition of DS-epi1while they had negligible effect on HS epimerase. The two inhibitors were able to reduce iduronic acid biosynthesis in CS/DS and GAG accumulation in WT and MPS-I fibroblasts. Docking of the inhibitors into DS-epi1 structure shows high affinity binding of both compounds to the active site. The collected data indicate that these hit compounds may be further elaborated to a potential lead drug used for attenuation of GAGs accumulation in MPS-I patients.


Asunto(s)
Inhibidores Enzimáticos , Fibroblastos , Glicosaminoglicanos , Mucopolisacaridosis I , Mucopolisacaridosis I/tratamiento farmacológico , Mucopolisacaridosis I/metabolismo , Mucopolisacaridosis I/patología , Humanos , Fibroblastos/metabolismo , Fibroblastos/efectos de los fármacos , Glicosaminoglicanos/metabolismo , Inhibidores Enzimáticos/farmacología , Inhibidores Enzimáticos/química , Carbohidrato Epimerasas/metabolismo , Carbohidrato Epimerasas/antagonistas & inhibidores , Carbohidrato Epimerasas/genética , Simulación del Acoplamiento Molecular , Antígenos de Neoplasias , Proteínas de Unión al ADN , Proteínas de Neoplasias
2.
Chembiochem ; 24(4): e202200619, 2023 02 14.
Artículo en Inglés | MEDLINE | ID: mdl-36453606

RESUMEN

1-Azasugar analogues of l-iduronic acid (l-IdoA) and d-glucuronic acid (d-GlcA) and their corresponding enantiomers have been synthesized as potential pharmacological chaperones for mucopolysaccharidosis I (MPS I), a lysosomal storage disease caused by mutations in the gene encoding α-iduronidase (IDUA). The compounds were efficiently synthesized in nine or ten steps from d- or l-arabinose, and the structures were confirmed by X-ray crystallographic analysis of key intermediates. All compounds were inactive against IDUA, although l-IdoA-configured 8 moderately inhibited ß-glucuronidase (ß-GLU). The d-GlcA-configured 9 was a potent inhibitor of ß-GLU and a moderate inhibitor of the endo-ß-glucuronidase heparanase. Co-crystallization of 9 with heparanase revealed that the endocyclic nitrogen of 9 forms close interactions with both the catalytic acid and catalytic nucleophile.


Asunto(s)
Iduronidasa , Mucopolisacaridosis I , Humanos , Iduronidasa/química , Iduronidasa/genética , Ácidos Urónicos , Glucuronidasa/química , Mucopolisacaridosis I/genética
3.
Int J Mol Sci ; 23(9)2022 Apr 27.
Artículo en Inglés | MEDLINE | ID: mdl-35563215

RESUMEN

Heparin is a polysaccharide expressed in animal connective tissue-type mast cells. Owing to the special pentasaccharide sequence, heparin specifically binds to antithrombin (AT) and increases the inhibitory activity of AT towards coagulation enzymes. Heparin isolated from porcine intestinal mucosa has an average molecular weight of 15 kDa, while heparins recovered from rat skin and the peritoneal cavity were 60-100 kDa and can be fragmented by the endo-glucuronidase heparanase in vitro. In this study, we have examined heparin isolated from in vitro matured fetal skin mast cells (FSMC) and peritoneal cavity mast cells (PCMC) collected from wildtype (WT), heparanase knockout (Hpa-KO), and heparanase overexpressing (Hpa-tg) mice. The metabolically 35S-labeled heparin products from the mast cells of WT, Hpa-KO, and Hpa-tg mice were compared and analyzed for molecular size and AT-binding activity. The results show that PCMC produced heparins with a size similar to heparin from porcine intestinal mast cells, whilst FSMC produced much longer chains. As expected, heparanase overexpression resulted in the generation of smaller fragments in both cell types, while heparins recovered from heparanase knockout cells were slightly longer than heparin from WT cells. Unexpectedly, we found that heparanase expression affected the production of total glycosaminoglycans (GAGs) and the proportion between heparin and other GAGs but essentially had no effect on heparin catabolism.


Asunto(s)
Glucuronidasa , Mastocitos , Animales , Anticoagulantes/metabolismo , Antitrombinas/metabolismo , Glucuronidasa/metabolismo , Glicosaminoglicanos/metabolismo , Heparina/química , Mastocitos/metabolismo , Ratones , Ratas , Porcinos
4.
Glycobiology ; 31(10): 1319-1329, 2021 11 18.
Artículo en Inglés | MEDLINE | ID: mdl-34192316

RESUMEN

Mucopolysaccharidosis type I (MPS-I) is a rare lysosomal storage disorder caused by deficiency of the enzyme alpha-L-iduronidase, which removes iduronic acid in both chondroitin/dermatan sulfate (CS/DS) and heparan sulfate (HS) and thereby contributes to the catabolism of glycosaminoglycans (GAGs). To ameliorate this genetic defect, the patients are currently treated by enzyme replacement and bone marrow transplantation, which have a number of drawbacks. This study was designed to develop an alternative treatment by inhibition of iduronic acid formation. By screening the Prestwick drug library, we identified ebselen as a potent inhibitor of enzymes that produce iduronic acid in CS/DS and HS. Ebselen efficiently inhibited iduronic acid formation during CS/DS synthesis in cultured fibroblasts. Treatment of MPS-I fibroblasts with ebselen not only reduced accumulation of CS/DS but also promoted GAG degradation. In early Xenopus embryos, this drug phenocopied the effect of downregulation of DS-epimerase 1, the main enzyme responsible for iduronic production in CS/DS, suggesting that ebselen inhibits iduronic acid production in vivo. However, ebselen failed to ameliorate the CS/DS and GAG burden in MPS-I mice. Nevertheless, the results propose a potential of iduronic acid substrate reduction therapy for MPS-I patients.


Asunto(s)
Fibroblastos/efectos de los fármacos , Glicosaminoglicanos/antagonistas & inhibidores , Ácido Idurónico/antagonistas & inhibidores , Isoindoles/farmacología , Mucopolisacaridosis I/tratamiento farmacológico , Compuestos de Organoselenio/farmacología , Relación Dosis-Respuesta a Droga , Fibroblastos/metabolismo , Fibroblastos/patología , Glicosaminoglicanos/metabolismo , Células HEK293 , Humanos , Ácido Idurónico/metabolismo , Isoindoles/química , Estructura Molecular , Mucopolisacaridosis I/metabolismo , Mucopolisacaridosis I/patología , Compuestos de Organoselenio/química , Relación Estructura-Actividad
5.
J Biol Chem ; 293(35): 13725-13735, 2018 08 31.
Artículo en Inglés | MEDLINE | ID: mdl-29976758

RESUMEN

During the biosynthesis of chondroitin/dermatan sulfate (CS/DS), a variable fraction of glucuronic acid is converted to iduronic acid through the activities of two epimerases, dermatan sulfate epimerases 1 (DS-epi1) and 2 (DS-epi2). Previous in vitro studies indicated that without association with other enzymes, DS-epi1 activity produces structures that have only a few adjacent iduronic acid units. In vivo, concomitant with epimerization, dermatan 4-O-sulfotransferase 1 (D4ST1) sulfates the GalNAc adjacent to iduronic acid. This sulfation facilitates DS-epi1 activity and enables the formation of long blocks of sulfated iduronic acid-containing domains, which can be major components of CS/DS. In this report, we used recombinant enzymes to confirm the concerted action of DS-epi1 and D4ST1. Confocal microscopy revealed that these two enzymes colocalize to the Golgi, and FRET experiments indicated that they physically interact. Furthermore, FRET, immunoprecipitation, and cross-linking experiments also revealed that DS-epi1, DS-epi2, and D4ST1 form homomers and are all part of a hetero-oligomeric complex where D4ST1 directly interacts with DS-epi1, but not with DS-epi2. The cooperation of DS-epi1 with D4ST1 may therefore explain the processive mode of the formation of iduronic acid blocks. In conclusion, the iduronic acid-forming enzymes operate in complexes, similar to other enzymes active in glycosaminoglycan biosynthesis. This knowledge shed light on regulatory mechanisms controlling the biosynthesis of the structurally diverse CS/DS molecule.


Asunto(s)
Antígenos de Neoplasias/metabolismo , Proteínas de Unión al ADN/metabolismo , Dermatán Sulfato/metabolismo , Ácido Idurónico/metabolismo , Proteínas de Neoplasias/metabolismo , Sulfotransferasas/metabolismo , Animales , Antígenos de Neoplasias/análisis , Células COS , Chlorocebus aethiops , Proteínas de Unión al ADN/análisis , Humanos , Proteínas de Neoplasias/análisis , Proteínas Recombinantes/análisis , Proteínas Recombinantes/metabolismo , Sulfotransferasas/análisis
6.
Glycobiology ; 29(6): 446-451, 2019 06 01.
Artículo en Inglés | MEDLINE | ID: mdl-30869126

RESUMEN

The glycosaminoglycan dermatan sulfate (DS) is a well-known activator of heparin cofactor II-dependent inactivation of thrombin. In contrast to heparin, dermatan sulfate has never been prepared recombinantly from material of non-animal origin. Here we report on the enzymatic synthesis of structurally well-defined DS with high anticoagulant activity. Using a microbial K4 polysaccharide and the recombinant enzymes DS-epimerase 1, dermatan 4-O-sulfotransferase 1, uronyl 2-O-sulfotransferase and N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, several new glycostructures have been prepared, such as a homogenously sulfated IdoA-GalNAc-4S polymer and its 2-O-, 6-O- and 2,6-O-sulfated derivatives. Importantly, the recombinant highly 2,4-O-sulfated DS inhibits thrombin via heparin cofactor II, approximately 20 times better than heparin, enabling manipulation of vascular and extravascular coagulation. The potential of this method can be extended to preparation of specific structures that are of importance for binding and activation of cytokines, and control of inflammation and metastasis, involving extravasation and migration.


Asunto(s)
Dermatán Sulfato/farmacología , Cofactor II de Heparina/metabolismo , Inhibidores de Serina Proteinasa/farmacología , Trombina/antagonistas & inhibidores , Conformación de Carbohidratos , Dermatán Sulfato/síntesis química , Dermatán Sulfato/química , Humanos , Modelos Moleculares , Inhibidores de Serina Proteinasa/síntesis química , Inhibidores de Serina Proteinasa/química , Trombina/metabolismo
7.
Development ; 142(6): 1146-58, 2015 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-25758225

RESUMEN

Germ layer formation and primary axis development rely on Fibroblast growth factors (FGFs). In Xenopus, the secreted serine protease HtrA1 induces mesoderm and posterior trunk/tail structures by facilitating the spread of FGF signals. Here, we show that the serpin Protease nexin-1 (PN1) is transcriptionally activated by FGF signals, suppresses mesoderm and promotes head development in mRNA-injected embryos. An antisense morpholino oligonucleotide against PN1 has the opposite effect and inhibits ectodermal fate. However, ectoderm and anterior head structures can be restored in PN1-depleted embryos when HtrA1 and FGF receptor activities are diminished, indicating that FGF signals negatively regulate their formation. We show that PN1 binds to and inhibits HtrA1, prevents degradation of the proteoglycan Syndecan 4 and restricts paracrine FGF/Erk signaling. Our data suggest that PN1 is a negative-feedback regulator of FGF signaling and has important roles in ectoderm and head development.


Asunto(s)
Tipificación del Cuerpo/fisiología , Retroalimentación Fisiológica/fisiología , Factores de Crecimiento de Fibroblastos/metabolismo , Estratos Germinativos/embriología , Serpina E2/metabolismo , Transducción de Señal/fisiología , Xenopus/embriología , Animales , Immunoblotting , Inmunoprecipitación , Hibridación in Situ
9.
Mol Med ; 22: 147-155, 2016 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-26933843

RESUMEN

Methionine adenosyltransferase (MAT) I/III deficiency can be inherited as autosomal dominant (AD) or as recessive (AR) traits in which mono- or biallelic MAT1A mutations have been identified, respectively. Although most patients have benign clinical outcomes, some with the AR form have neurological deficits. Here we describe 16 Korean patients with MAT I/III deficiency from 15 unrelated families identified by newborn screening. Ten probands had the AD MAT I/III deficiency, while six had AR MAT I/III deficiency. Plasma methionine (145.7 µmol/L versus 733.2 µmol/L, P < 0.05) and homocysteine levels (12.3 µmol/L versus 18.6 µmol/L, P < 0.05) were lower in the AD type than in AR type. In addition to the only reported AD MAT1A mutation, p.Arg264His, we identified two novel AD mutations, p.Arg249Gln and p.Gly280Arg. In the AR type, four previously reported and two novel mutations, p.Arg163Trp and p.Tyr335*, were identified. No exonic deletions were found by quantitative genomic polymerase chain reaction (PCR). Three-dimensional structural prediction programs indicated that the AD-type mutations were located on the dimer interface or in the substrate binding site, hindering MAT I/III dimerization or substrate binding, respectively, whereas the AR mutations were distant from the interface or substrate binding site. These results indicate that the AD or AR MAT I/III deficiency is correlated with clinical findings, substrate levels and structural features of the mutant proteins, which is important for the neurological management and genetic counseling of the patients.

10.
Glycobiology ; 25(1): 30-41, 2015 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-25138304

RESUMEN

Proteoglycan (PG) sulfation depends on activated nucleotide sulfate, 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Transporters in the Golgi membrane translocate PAPS from the cytoplasm into the organelle lumen where PG sulfation occurs. Silencing of PAPS transporter (PAPST) 1 in epithelial MDCK cells reduced PAPS uptake into Golgi vesicles. Surprisingly, at the same time sulfation of heparan sulfate (HS) was stimulated. The effect was pathway specific in polarized epithelial cells. Basolaterally secreted proteoglycans (PGs) displayed an altered HS sulfation pattern and increased growth factor binding capacity. In contrast, the sulfation pattern of apically secreted PGs was unchanged while the secretion was reduced. Regulation of PAPST1 allows epithelial cells to prioritize between PG sulfation in the apical and basolateral secretory routes at the level of the Golgi apparatus. This provides sulfation patterns that ensure PG functions at the extracellular level, such as growth factor binding.


Asunto(s)
Sulfatos de Condroitina/metabolismo , Aparato de Golgi/metabolismo , Proteoglicanos de Heparán Sulfato/metabolismo , Heparitina Sulfato/metabolismo , Proteínas de Transporte de Membrana/metabolismo , Fosfoadenosina Fosfosulfato/metabolismo , Animales , Transporte Biológico , Polaridad Celular , Sulfatos de Condroitina/química , Perros , Regulación de la Expresión Génica , Proteoglicanos de Heparán Sulfato/química , Heparitina Sulfato/química , Péptidos y Proteínas de Señalización Intercelular/genética , Péptidos y Proteínas de Señalización Intercelular/metabolismo , Células de Riñón Canino Madin Darby , Proteínas de Transporte de Membrana/genética , Fosfoadenosina Fosfosulfato/química , Unión Proteica , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/metabolismo
11.
Birth Defects Res A Clin Mol Teratol ; 100(9): 712-20, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25186462

RESUMEN

BACKGROUND: Dermatan sulfate (DS) is a highly sulfated polysaccharide with a variety of biological functions in extracellular matrix organization and processes such as tumorigenesis and wound healing. A distinct feature of DS is the presence of iduronic acid, produced by the two enzymes, DS-epimerase 1 and 2, which are encoded by Dse and Dsel, respectively. METHODS: We have previously shown that Dse knockout (KO) mice in a mixed C57BL/6-129/SvJ background have an altered collagen matrix structure in skin. In the current work we studied Dse KO mice in a pure NFR genetic background. RESULTS: Dse KO embryos and newborns had kinked tails and histological staining revealed significantly thicker epidermal layers in Dse KO mice when compared with heterozygote (Het) or wild-type (WT) littermates. Immunochemical analysis of the epidermal layers in newborn pups showed increased expression of keratin 5 in the basal layer and keratin 1 in the spinous layer. In addition, we observed an abdominal wall defect with herniated intestines in 16% of the Dse KO embryos. Other, less frequent, developmental defects were exencephaly and spina bifida. CONCLUSION: We conclude that the combination of defective collagen structure in the dermis and imbalanced keratinocyte maturation could be responsible for the observed developmental defects in Dse KO mice. In addition, we propose that Dse KO mice could be used as a model in pathogenetic studies of human fetal abdominal wall defects.


Asunto(s)
Pared Abdominal/anomalías , Carbohidrato Epimerasas/genética , Dermis/metabolismo , Hernia Abdominal/genética , Queratinocitos/metabolismo , Animales , Carbohidrato Epimerasas/deficiencia , Dermatán Sulfato/metabolismo , Dermis/patología , Modelos Animales de Enfermedad , Embrión de Mamíferos , Expresión Génica , Hernia Abdominal/complicaciones , Hernia Abdominal/patología , Humanos , Queratina-1/genética , Queratina-1/metabolismo , Queratina-15/genética , Queratina-15/metabolismo , Queratinocitos/patología , Ratones , Ratones Noqueados , Defectos del Tubo Neural/complicaciones , Defectos del Tubo Neural/genética , Defectos del Tubo Neural/patología , Disrafia Espinal/complicaciones , Disrafia Espinal/genética , Disrafia Espinal/patología
12.
Intensive Care Med Exp ; 11(1): 63, 2023 Sep 21.
Artículo en Inglés | MEDLINE | ID: mdl-37733256

RESUMEN

Sepsis is a condition with high morbidity and mortality. Prompt recognition and initiation of treatment is essential. Despite forming an integral part of sepsis management, fluid resuscitation may also lead to volume overload, which in turn is associated with increased mortality. The optimal fluid strategy in sepsis resuscitation is yet to be defined. Hyaluronan, an endogenous glycosaminoglycan with high affinity to water is an important constituent of the endothelial glycocalyx. We hypothesized that exogenously administered hyaluronan would counteract intravascular volume depletion and contribute to endothelial glycocalyx integrity in a fluid restrictive model of peritonitis. In a prospective, blinded model of porcine peritonitis sepsis, we randomized animals to intervention with hyaluronan (n = 8) or 0.9% saline (n = 8). The animals received an infusion of 0.1% hyaluronan 6 ml/kg/h, or the same volume of saline, during the first 2 h of peritonitis. Stroke volume variation and hemoconcentration were comparable in the two groups throughout the experiment. Cardiac output was higher in the intervention group during the infusion of hyaluronan (3.2 ± 0.5 l/min in intervention group vs 2.7 ± 0.2 l/min in the control group) (p = 0.039). The increase in lactate was more pronounced in the intervention group (3.2 ± 1.0 mmol/l in the intervention group and 1.7 ± 0.7 mmol/l in the control group) at the end of the experiment (p < 0.001). Concentrations of surrogate markers of glycocalyx damage; syndecan 1 (0.6 ± 0.2 ng/ml vs 0.5 ± 0.2 ng/ml, p = 0.292), heparan sulphate (1.23 ± 0.2 vs 1.4 ± 0.3 ng/ml, p = 0.211) and vascular adhesion protein 1 (7.0 ± 4.1 vs 8.2 ± 2.3 ng/ml, p = 0.492) were comparable in the two groups at the end of the experiment. In conclusion, hyaluronan did not counteract intravascular volume depletion in early peritonitis sepsis. However, this finding is hampered by the short observation period and a beneficial effect of HMW-HA in peritonitis sepsis cannot be discarded based on the results of the present study.

13.
Carbohydr Polym ; 299: 120191, 2023 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-36876765

RESUMEN

Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues. Incubations of a [4GlcAß1-4GlcNSO3α1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps. Enzyme complexes were supported by computational modeling and homogeneous time resolved fluorescence. GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions. Evidence for a functional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O-sulfated glucosamine residues. The inability to achieve simultaneous 2-O- and 6-O-sulfation in vitro supported topologically separated reactions in the cell. These findings provide novel insight into the roles of enzyme interactions in heparan sulfate biosynthesis.


Asunto(s)
Ácido Idurónico , Complejos Multienzimáticos , Ácido Glucurónico , Polímeros , Protones , Racemasas y Epimerasas , Sulfotransferasas , Heparitina Sulfato
14.
Glycobiology ; 22(7): 1007-16, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-22496542

RESUMEN

Dermatan sulfate epimerase 2 (DS-epi2), together with its homolog DS-epi1, transform glucuronic acid into iduronic acid in DS polysaccharide chains. Iduronic acid gives DS increased chain flexibility and promotes protein binding. DS-epi2 is ubiquitously expressed and is the predominant epimerase in the brain. Here, we report the generation and initial characterization of DS-epi2 null mice. DS-epi2-deficient mice showed no anatomical, histological or morphological abnormalities. The body weights and lengths of mutated and wild-type littermates were indistinguishable. They were fertile and had a normal lifespan. Chondroitin sulfate (CS)/DS isolated from the newborn mutated mouse brains had a 38% reduction in iduronic acid compared with wild-type littermates, and compositional analysis revealed a decrease in 4-O-sulfate and an increase in 6-O-sulfate containing structures. Despite the reduction in iduronic acid, the adult DS-epi2-/- brain showed normal extracellular matrix features by immunohistological stainings. We conclude that DS-epi1 compensates in vivo for the loss of DS-epi2. These results extend previous findings of the functional redundancy of brain extracellular matrix components.


Asunto(s)
Encéfalo/crecimiento & desarrollo , Carbohidrato Epimerasas/deficiencia , Dermatán Sulfato/metabolismo , Animales , Encéfalo/citología , Encéfalo/enzimología , Conformación de Carbohidratos , Carbohidrato Epimerasas/genética , Células Cultivadas , Disacáridos/metabolismo , Matriz Extracelular/metabolismo , Femenino , Técnicas de Inactivación de Genes , Ácido Idurónico/metabolismo , Riñón/metabolismo , Masculino , Ratones , Ratones de la Cepa 129 , Ratones Endogámicos C57BL , Ratones Transgénicos , Sulfatos/metabolismo
15.
Dev Cell ; 13(2): 226-41, 2007 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-17681134

RESUMEN

We found that the secreted serine protease xHtrA1, expressed in the early embryo and transcriptionally activated by FGF signals, promotes posterior development in mRNA-injected Xenopus embryos. xHtrA1 mRNA led to the induction of secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner. An antisense morpholino oligonucleotide or a neutralizing antibody against xHtrA1 had the opposite effects. xHtrA1 activates FGF/ERK signaling and the transcription of FGF genes. We show that Xenopus Biglycan, Syndecan-4, and Glypican-4 are proteolytic targets of xHtrA1 and that heparan sulfate and dermatan sulfate trigger posteriorization, mesoderm induction, and neuronal differentiation via the FGF signaling pathway. The results are consistent with a mechanism by which xHtrA1, through cleaving proteoglycans, releases cell-surface-bound FGF ligands and stimulates long-range FGF signaling.


Asunto(s)
Embrión no Mamífero/enzimología , Factores de Crecimiento de Fibroblastos/metabolismo , Serina Endopeptidasas/metabolismo , Transducción de Señal , Proteínas de Xenopus/metabolismo , Xenopus laevis/embriología , Xenopus laevis/metabolismo , Animales , Secuencia de Bases , Biglicano , Tipificación del Cuerpo/efectos de los fármacos , Bovinos , Diferenciación Celular/efectos de los fármacos , Dermatán Sulfato/farmacología , Ectodermo/efectos de los fármacos , Ectodermo/metabolismo , Embrión no Mamífero/efectos de los fármacos , Proteínas de la Matriz Extracelular/metabolismo , Factor 8 de Crecimiento de Fibroblastos/metabolismo , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Glipicanos/metabolismo , Heparitina Sulfato/farmacología , Humanos , Mesodermo/efectos de los fármacos , Mesodermo/metabolismo , Datos de Secuencia Molecular , Neuronas/citología , Neuronas/efectos de los fármacos , Procesamiento Proteico-Postraduccional/efectos de los fármacos , Proteoglicanos/metabolismo , Serina Endopeptidasas/química , Serina Endopeptidasas/genética , Transducción de Señal/efectos de los fármacos , Sindecano-4/metabolismo , Cola (estructura animal)/anatomía & histología , Cola (estructura animal)/efectos de los fármacos , Cola (estructura animal)/embriología , Proteínas de Xenopus/química , Proteínas de Xenopus/genética
16.
Glycobiology ; 21(5): 565-74, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21177331

RESUMEN

Chondroitin sulfate (CS) and dermatan sulfate (DS) are expressed in significant amounts in the brain and play important roles in the development of the central nervous system in mammals. CS and DS structures are often found in a single CS/DS hybrid chain. The l-iduronic acid (IdoA)-containing domain, which defines a DS-type domain, appears key to the biological functions of the CS/DS hybrid chain. In this study, to clarify the distribution of the DS-type structure in the brain during development, the expression patterns of DS epimerase 1 (DS-epi1) and DS-epi2, both of which convert d-glucuronic acid into IdoA, were investigated by in situ hybridization. DS-epi2 was ubiquitously expressed in the developing brain after birth, whereas the expression of DS-epi1 was faint and obscure at all developmental stages. Quantitative real-time polymerase chain reaction revealed the expression of DS-epi2 to be higher than that of DS-epi1 throughout development, suggesting that DS-epi2 but not DS-epi1 is mostly expressed in the brain and plays key roles in the epimerization of CS/DS during its biosynthesis. Moreover, an analysis of the disaccharides of CS/DS demonstrated significant amounts of IdoA-containing iD units [IdoA(2S)-GalNAc(6S)] and iB units [IdoA(2S)-GalNAc(4S)], where 2S, 4S and 6S stand for 2-O-, 4-O- and 6-O-sulfate, respectively, in every region of the brain examined. The proportion of these units in cerebellar CS/DS was greatly altered during postnatal development. These results suggest that the IdoA-containing structures in the developing brain are mainly produced by the actions of DS-epi2 and play crucial roles in postnatal development.


Asunto(s)
Encéfalo/metabolismo , Carbohidrato Epimerasas/metabolismo , Sulfatos de Condroitina/metabolismo , Dermatán Sulfato/metabolismo , Animales , Encéfalo/anatomía & histología , Encéfalo/crecimiento & desarrollo , Carbohidrato Epimerasas/genética , Disacaridasas/metabolismo , Regulación del Desarrollo de la Expresión Génica , Ácido Idurónico/metabolismo , Hibridación in Situ , Isoenzimas , Ratones , ARN Mensajero/metabolismo , Transcripción Genética
17.
Glycobiology ; 19(11): 1197-203, 2009 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-19661164

RESUMEN

Chondroitin/dermatan sulfate is a highly complex linear polysaccharide ubiquitously found in the extracellular matrix and at the cell surface. Several of its functions, such as binding to growth factors, are mediated by domains composed of alternating iduronic acid and 4-O-sulfated N-acetylgalactosamine residues, named 4-O-sulfated iduronic acid blocks. These domains are generated by the action of two DS-epimerases, which convert D-glucuronic acid into its epimer L-iduronic acid, in close connection with 4-O-sulfation. In this study, dermatan sulfate structure was evaluated after downregulating or increasing dermatan 4-O-sulfotransferase 1 (D4ST-1) expression. siRNA-mediated downregulation of D4ST-1 in primary human lung fibroblasts led to a drastic specific reduction of iduronic acid blocks. No change of epimerase activity was found, indicating that the influence of D4ST-1 on epimerization is not due to an altered expression level of the DS-epimerases. Analysis of the dermatan sulfate chains showed that D4ST-1 is essential for the biosynthesis of the disulfated structure iduronic acid-2-O-sulfate-N-acetylgalactosamine-4-O-sulfate, thus confirmed to be strictly connected with the iduronic acid blocks. Also the biologically important residue hexuronic acid-N-acetylgalactosamine-4,6-O-disulfate considerably decreased after D4ST-1 downregulation. In conclusion, D4ST-1 is a key enzyme and is indispensable in the formation of important functional domains in dermatan sulfate and cannot be compensated by other 4-O-sulfotransferases.


Asunto(s)
Dermatán Sulfato/química , Dermatán Sulfato/metabolismo , Ácido Idurónico/metabolismo , Sulfotransferasas/metabolismo , Dermatán Sulfato/biosíntesis , Regulación hacia Abajo/efectos de los fármacos , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Fibroblastos/enzimología , Humanos , Pulmón/citología , ARN Interferente Pequeño/farmacología , Sulfotransferasas/biosíntesis , Sulfotransferasas/genética
18.
PLoS One ; 13(1): e0191751, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29370293

RESUMEN

Chondroitin sulfate (CS)/dermatan sulfate (DS) proteoglycans are abundant on the cell surface and in the extracellular matrix and have important functions in matrix structure, cell-matrix interaction and signaling. The DS epimerases 1 and 2, encoded by Dse and Dsel, respectively, convert CS to a CS/DS hybrid chain, which is structurally and conformationally richer than CS, favouring interaction with matrix proteins and growth factors. We recently showed that Xenopus Dse is essential for the migration of neural crest cells by allowing cell surface CS/DS proteoglycans to adhere to fibronectin. Here we investigate the expression of Dse and Dsel in Xenopus embryos. We show that both genes are maternally expressed and exhibit partially overlapping activity in the eyes, brain, trigeminal ganglia, neural crest, adenohypophysis, sclerotome, and dorsal endoderm. Dse is specifically expressed in the epidermis, anterior surface ectoderm, spinal nerves, notochord and dermatome, whereas Dsel mRNA alone is transcribed in the spinal cord, epibranchial ganglia, prechordal mesendoderm and myotome. The expression of the two genes coincides with sites of cell differentiation in the epidermis and neural tissue. Several expression domains can be linked to previously reported phenotypes of knockout mice and clinical manifestations, such as the Musculocontractural Ehlers-Danlos syndrome and psychiatric disorders.


Asunto(s)
Carbohidrato Epimerasas/genética , Regulación del Desarrollo de la Expresión Génica , Xenopus laevis/embriología , Animales , Encéfalo/metabolismo , Hibridación in Situ , Sondas ARN , ARN Mensajero/genética
19.
Front Immunol ; 9: 206, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29472931

RESUMEN

For full activation of naïve adaptive lymphocytes in skin-draining lymph nodes (LNs), presentation of peptide:MHC complexes by LN-resident and skin-derived dendritic cells (DCs) that encountered antigens (Ags) is an absolute prerequisite. To get to the nearest draining LN upon intradermal immunization, DCs need to migrate from the infection site to the afferent lymphatics, which can only be reached by traversing a collagen-dense network located in the dermis of the skin through the activity of proteolytic enzymes. Here, we show that mice with altered collagen fibrillogenesis resulting in thicker collagen fibers in the skin display a reduced DC migration to the draining LN upon immune challenge. Consequently, the initiation of the cellular and humoral immune response was diminished. Ag-specific CD8+ and CD4+ T cells as well as Ag-specific germinal center B cells and serum immunoglobulin levels were significantly decreased. Hence, we postulate that alterations to the production of extracellular matrix, as seen in various connective tissue disorders, may in the end affect the qualitative outcome of adaptive immunity.


Asunto(s)
Inmunidad Adaptativa , Movimiento Celular/inmunología , Dermatán Sulfato/metabolismo , Células de Langerhans/inmunología , Ganglios Linfáticos/inmunología , Animales , Biopsia , Linfocitos T CD8-positivos/inmunología , Carbohidrato Epimerasas/deficiencia , Carbohidrato Epimerasas/genética , Dermatán Sulfato/inmunología , Femenino , Células de Langerhans/metabolismo , Ganglios Linfáticos/citología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Modelos Animales , Piel/citología , Piel/inmunología , Piel/patología
20.
Orphanet J Rare Dis ; 13(1): 4, 2018 01 10.
Artículo en Inglés | MEDLINE | ID: mdl-29321044

RESUMEN

BACKGROUND: TMEM199 deficiency was recently shown in four patients to cause liver disease with steatosis, elevated serum transaminases, cholesterol and alkaline phosphatase and abnormal protein glycosylation. There is no information on the long-term outcome in this disorder. RESULTS: We here present three novel patients with TMEM199-CDG. All three patients carried the same set of mutations (c.13-14delTT (p.Ser4Serfs*30) and c.92G > C (p.Arg31Pro), despite only two were related (siblings). One mutation (c.92G > C) was described previously whereas the other was deemed pathogenic due to its early frameshift. Western Blot analysis confirmed a reduced level of TMEM199 protein in patient fibroblasts and all patients showed a similar glycosylation defect. The patients presented with a very similar clinical and biochemical phenotype to the initial publication, confirming that TMEM199-CDG is a non-encephalopathic liver disorder. Two of the patients were clinically assessed over two decades without deterioration. CONCLUSION: A rising number of disorders affecting Golgi homeostasis have been published over the last few years. A hallmark finding is deficiency in protein glycosylation, both in N- and O-linked types. Most of these disorders have signs of both liver and brain involvement. However, the present and the four previously reported patients do not show encephalopathy but a chronic, non-progressive (over decades) liver disease with hypertransaminasemia and steatosis. This information is crucial for the patient/families and clinician at diagnosis, as it distinguishes it from other Golgi homeostasis disorders, in having a much more favorable course.


Asunto(s)
Hepatopatías/metabolismo , Proteínas de la Membrana/metabolismo , Fosfatasa Alcalina/genética , Fosfatasa Alcalina/metabolismo , Ceruloplasmina/genética , Ceruloplasmina/metabolismo , Preescolar , Trastornos Congénitos de Glicosilación/genética , Trastornos Congénitos de Glicosilación/metabolismo , Femenino , Glicosilación , Aparato de Golgi/genética , Aparato de Golgi/metabolismo , Humanos , Hígado/metabolismo , Hepatopatías/genética , Masculino , Proteínas de la Membrana/genética , Mutación , Transferrina/genética , Transferrina/metabolismo , Adulto Joven
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