High-sensitivity operation of an unshielded single cell radio-frequency atomic magnetometer: erratum.

An erratum is presented to include the right vertical scale and label in Fig. 2 and Fig. 7, which were omitted in our published manuscript ["High-sensitivity operation of an unshielded single cell radio-frequency atomic magnetometer" Opt. Express30 (23), 42015 (2022) 10.1364/OE.476016].

Neural network-aided optimisation of a radio-frequency atomic magnetometer.

Efficient unsupervised optimisation of atomic magnetometers is a requirement in many applications, where direct intervention of an operator is not feasible. The efficient extraction of the optimal operating conditions from a small sample of experimental data requires a robust automated regression of the available data. Here we address this issue and propose the use of general regression neural networks as a tool for the optimisation of atomic magnetometers which does not require human supervision and is efficient, as it is ideally suited to operating with a small sample of data as input. As a case study, we specifically demonstrate the optimisation of an unshielded radio-frequency atomic magnetometer by using a general regression neural network which establishes a mapping between three input variables, the cell temperature, the pump beam power and the probe beam power, and one output variable, the AC sensitivity. The optimisation results into an AC sensitivity of 44 fT/Hz at 26 kHz.

High-sensitivity operation of an unshielded single cell radio-frequency atomic magnetometer.

Real-world applications of atomic magnetometers require the ability to operate them with high-sensitivity in the presence of magnetic noise. In the present work, high-sensitivity operation of unshielded atomic magnetometers in a magnetically noisy environment is demonstrated. The distinguishing feature of the demonstrated approach is the implementation of active in-situ bias field stabilization using multiple fluxgate magnetometers. This is combined with the use of a counter-propagating pump and triple-pass probe configuration, to maximize the atomic polarization and the probe rotation respectively, so to reach high-sensitivity. The improvement in sensitivity of the unshielded system with respect to previous realizations is fully characterized, with the contributions of the different modifications of the apparatus individually quantified. The presented set-up is suitable for the detection of long-range magnetic fields, where shielding or differential measurements using multi-sensor set-ups do not constitute viable options.

Optimisation of a radio-frequency atomic magnetometer: a Uniform Design approach.

High-sensitivity operation of a radio-frequency atomic magnetometer (RF-AM) requires careful setting of the system parameters, including the lasers intensity and detuning, and the vapour cell temperature. The identification of the optimal operating parameters, which ensures high sensitivity, is typically performed empirically and is often a lengthy process, which is especially labour intensive if frequent retuning of the magnetometer is required to perform different tasks. This paper demonstrates an efficient approach to RF-AM performance optimisation which relies on an open-loop optimisation technique based on Uniform Design (UD). This paper specifically describes the optimisation of an unshielded RF-AM based on a 4-factor-12-level UD of the experimental parameters space. The proposed procedure is shown to lead to the efficient optimisation of the atomic magnetometer at different frequencies, and is applicable to both AC and DC sensitivity optimisation. The procedure does not require any detailed knowledge of the model underlying the operation of the RF-AM and is effective in reducing the number of experimental runs required for the optimisation. It is ideally suited to self-calibration of devices without human supervision.

Targeted annotation of immunoglobulin light chain (IgL) genes in zebrafish from BAC clones reveals kappa-like recombining/deleting elements within IgL constant regions.

Genomic organization, composition, and microsynteny of immunoglobulin light chain (IgL) gene segments in the zebrafish were analyzed through the identification and annotation of overlapping BAC clone insert sequences and an Illumina de novo assembly. The resultant gap-free IgL annotation confirmed a number of previous conclusions about teleost IgL including: suites of (V(L)-J(L)-C(L)) clusters on multiple chromosomes; V(L) in the same or opposite transcriptional orientation as J(L) and C(L); and the apparent absence of lambda IgL in the zebrafish model. In addition, palindromic heptamers (CACAGTG or CACTGTG) within the 3' region of zebrafish C(L) were identified. In mammals, heptamers within J(κ)-C(κ) introns can recombine with downstream kappa deleting elements (Kde) to ablate C(κ) regions prior to rearrangements of V(λ)-J(λ) gene segments. The presence of palindromic heptamers within zebrafish C(L) is intriguing as their recombination with intact RSS might result in the deletion of a large portion of the C(L) thereby permanently silencing C(L) exons within the IgL locus. Given that bony fish have appreciably more C(L) spread over more chromosomes than mice and humans, it is plausible the presence of recombining sequences within C(L) may be tied to a need for heightened mechanisms to facilitate allelic exclusion or receptor editing. Collectively, with this report, gap-free annotations of the heavy and light chain Ig loci have now been completed for Danio rerio, the only teleost for which this has been accomplished, thereby strengthening the overall utility of zebrafish as a model organism for both comparative immunology and biomedical research.

Immunoglobulin light chain (IgL) genes in zebrafish: Genomic configurations and inversional rearrangements between (V(L)-J(L)-C(L)) gene clusters.

In mammals, Immunoglobulin light chain (IgL) are localized to two chromosomal regions (designated kappa and lambda). Here we report a genome-wide survey of IgL genes in the zebrafish revealing (V(L)-J(L)-C(L)) clusters spanning 5 separate chromosomes. To elucidate IgL loci present in the zebrafish genome assembly (Zv6), conventional sequence similarity searches and a novel scanning approach based on recombination signal sequence (RSS) motifs were applied. RT-PCR with zebrafish cDNA was used to confirm annotations, evaluate VJ-rearrangement possibilities and show that each chromosomal locus is expressed. In contrast to other vertebrates in which IgL exon usage has been studied, inversional rearrangement between (V(L)-J(L)-C(L)) clusters were found. Inter-cluster rearrangements may convey a selective advantage for editing self-reactive receptors and poise zebrafish by virtue of their extensive numbers of V(L), J(L) and C(L) to have greater potential for immunoglobulin gene shuffling than traditionally studied mice and human models.