[Psycho-pathological risk factor of arterial disease (prevalence, superposition or vinculation with other factors)]. / Estudio oncativo: factores de riesgo psicopatológicos de enfermedad arterial (prevalencia, superposición o vínculo con otros factores).

Alexitimia and depression may or not coexist with others risk factors (comportment o physical). Frecuently they have relation with socio-echonomic status and with ethnia. Sometimes are determinants of the atherosclerotic process by increasing the vascular reactivity by the alteration of the evolution. There is no information in our country about this problem in general population. The present study result of the investigation of these aspects and the comportamental and physical factors of arterial disease, in a population of Cordoba province (Argentina Republic).

The effect of phospholipase A(2) immobilization upon calcium interaction: a kinetic study.

In this work we studied the effect of Ca(2+) on the ability of immobilized PLA(2) to hydrolyze phospholipid substrates either in aggregate or monomeric forms. We use a kinetic methodology for the determination of dissociation constants of soluble and immobilized PLA(2)-Ca(2+) complexes. This approach allows us to obtain the values of the dissociation constants of enzyme-Ca(2+) (K(x)) and enzyme-Ca(2+)-substrate (K'(x)) complexes from the kinetic data obtained at different substrate and Ca(2+) concentrations. Results using mixed micelles of phospholipid-Triton X-100 showed that, in most cases, productive complexes were destabilized by immobilization of PLA(2). However, a correct analysis of the interaction must be independent of the classical modes of PLA(2) action toward lipid surfaces. Thus, a substrate in monomeric form was also employed to analyze the effect of immobilization on hydrolysis rate in the absence of interfacial activation. Kinetic data showed that the immobilization affected severely the mode of PLA(2) action. The kinetic data also suggested that immobilization promoted conformational alterations in the Ca(2+)-binding site, destabilizing the productive complex enzyme-Ca(2+)-phospholipid.

A simple method to obtain a covalent immobilized phospholipase A(2).

In the present work, we obtained an immobilized phospholipase A(2) system through covalent coupling by using an acrylic polymer Eupergit C as support. The immobilized enzyme from cobra venom (Naja naja naja) showed good retention activity and excellent stability. Both properties are of great importance for biomedical applications such as hypercholesterolemia treatments.