Evidence of reassortment of avian influenza A (H2) viruses in Brazilian shorebirds.

Influenza A viruses of the H2 subtype represent a zoonotic and pandemic threat to humans due to a lack of widespread specific immunity. Although A(H2) viruses that circulate in wild bird reservoirs are distinct from the 1957 pandemic A(H2N2) viruses, there is concern that they could impact animal and public health. There is limited information on AIVs in Latin America, and next to nothing about H2 subtypes in Brazil. In the present study, we report the occurrence and genomic sequences of two influenza A viruses isolated from wild-caught white-rumped sandpipers (Calidris fuscicollis). One virus, identified as A(H2N1), was isolated from a bird captured in Restinga de Jurubatiba National Park (PNRJ, Rio de Janeiro), while the other, identified as A(H2N2), was isolated from a bird captured in Lagoa do Peixe National Park (PNLP, Rio Grande do Sul). DNA sequencing and phylogenetic analysis of the obtained sequences revealed that each virus belonged to distinct subtypes. Furthermore, the phylogenetic analysis indicated that the genomic sequence of the A(H2N1) virus isolated from PNRJ was most closely related to other A(H2N1) viruses isolated from North American birds. On the other hand, the A(H2N2) virus genome recovered from the PNLP-captured bird exhibited a more diverse origin, with some sequences closely related to viruses from Iceland and North America, and others showing similarity to virus sequences recovered from birds in South America. Viral genes of diverse origins were identified in one of the viruses, indicating local reassortment. This suggests that the extreme South of Brazil may serve as an environment conducive to reassortment between avian influenza virus lineages from North and South America, potentially contributing to an increase in overall viral diversity.

Comparing photography and collection methods to sample litter in seabird nests in a coastal archipelago in the Southwest Atlantic.

Different methods are used to quantify and classify litter in seabird nests, such as the collection method (CM) and the photography method (PM). We compared the CM and PM in 195 brown booby (Sula leucogaster) nests breeding in a coastal archipelago in the state of Rio de Janeiro, Brazil. Photographs recorded 109 litter items in 44 nests (23% of nests), compared to 416 litter items in 82 nests (42%) by the CM. Pairwise comparison showed a significant difference in the variety and amount of litter items per nest, which was greater for CM (2.1 ± 1.1 categories, 2.13 ± 4.8 items) than for PM (1.5 ± 0.8 categories; 0.56 ± 1.6 items), in addition to a significant difference in the overall litter composition. The CM has been the most often used method to date. Although PM underestimates the amount and frequency of litter, we encourage its use when litter is abundant in nests and for threatened species.

Gillnet fisheries as a major mortality factor of Magellanic penguins in wintering areas.

The incidental capture in fisheries is probably the main conservation problem affecting seabirds. While the capture of albatrosses and petrels on longline hooks is well-known worldwide, the bycatch of diving seabirds in gillnets is an overlooked conservation problem. During a winter coastal fishing trip, the capture of Magellanic penguins (Spheniscus magellanicus) was recorded in driftnet and bottom setnet fisheries for the first time in southern Brazil. The highest captures rates were found in driftnets, from 146.5 to 545.5 penguins/km² of net and a total of 56 dead penguins were recorded. In the bottom gillnet, a total of 12 birds were killed and the capture rates varied from 41.7 to 125.0 penguins/km² of net. Although preliminary, the results presented in this paper were consistent between sets. If we consider the magnitude of driftnet and setnet fishing fleets, and that most dead penguins were adults, the impact upon Magellanic penguin populations is probably significant.