RESUMEN
In this study, two slightly different halophilic mixed exoelectrogens were enriched and selected from salt lake soils. The results showed that the selected mixed exoelectrogens ESA from the sample OSA (Xiaochaidan Lake soil) and ESB from the sample OSB (Dachaidan Lake soil), without additional NaCl, produced current densities of 1231.1 and 1050.2 µA cm-2, which were 89.6% and 61.7% higher than the typical exoelectrogen G. sulfurreducens PCA, respectively. ESA and ESB could produce 2.7 and 1.9 times higher currents than that obtained using G. sulfurreducens PCA with an additional 1.5% NaCl, respectively. The community diversity data demonstrated that Proteobacteria was the most abundant phylum, in which Enterobacteriaceae and Rhodocyclaceae were the dominant families for both ESA and ESB. Furthermore, at the genus level, the dominant genera Propionivibrio and Escherichia-Shigella were also shared by both. ESA had higher species diversity compared to ESB.
RESUMEN
Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1% growth inhibition against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen of tomato. For enhanced production of antifungal metabolites, nutrient optimization was performed and in vitro well-diffusion antifungal assays demonstrated that crude extract obtained from GPYB culture showed a maximum zone of inhibition (8.8 ± 0.4 mm) against the wilt pathogen, which is corroborated by the comparative LCMS profiles of the extracts from all three media i.e. GPYB, YEB and PDB. Two known compounds, Asperazine (m/z 665 [M + H]+) and Nigerone (m/z 571 [M + H]+), were isolated from A. niger and their antifungal activity is reported here for the first time. In MIC experiments, Asperazine and Nigerone inhibited the pathogen at 60 and 80 µg·mL-1 respectively. Molecular docking studies of Nigerone and Asperazine with F. oxysporum tomatinase showed five and six binding interactions respectively.