Engineering 4-coumaroyl-CoA derived polyketide production in Yarrowia lipolytica through a ß-oxidation mediated strategy.

Polyketides are a diverse class of molecules sought after for their valuable properties, including as potential pharmaceuticals. Previously, we demonstrated that the oleaginous yeast Yarrowia lipolytica is an optimal host for production of the simple polyketide, triacetic acid lactone (TAL). We here expand the capacities of this host by overcoming previous media challenges and enabling production of more complex polyketides. Specifically, we employ a ß-oxidation related strategy to improve polyketide production directly from defined media. Beyond TAL production, we establish biosynthesis of the 4-coumaroyl-CoA derived polyketides: naringenin, resveratrol, and bisdemethoxycurcumin, as well as the diketide intermediate, (E)-5-(4-hydroxyphenyl)-3-oxopent-4-enoic acid. In this background, we enable high-level de novo production of naringenin through import of both a heterologous pathway and a mutant Y. lipolytica allele. In doing so, we generated an averaged maximum titer of 898 mg/L naringenin, the highest titer reported to date in any host. These results demonstrate that Y. lipolytica is an ideal polyketide production host for more complex 4-coumaroyl-CoA derived products.

Yarrowia lipolytica: more than an oleaginous workhorse.

Microbial production of fuels and chemicals offers a means by which sustainable product manufacture can be achieved. In this regard, Yarrowia lipolytica is a unique microorganism suitable for a diverse array of biotechnological applications. As a robust oleaginous yeast, it has been well studied for production of fuels and chemicals derived from fatty acids. However, thanks in part to newfound genetic tools and metabolic understanding, Y. lipolytica has been explored for high-level production of a variety of non-lipid products. This mini-review will discuss some of the recent research surrounding the ability of Y. lipolytica to support bio-based chemical production outside the realm of fatty acid metabolism including polyketides, terpenes, carotenoids, pentose phosphate-derived products, polymers, and nanoparticles.

Bark in the park: a review of domestic dogs in parks.

The presence of domestic dogs Canis familiaris in public open spaces is increasingly controversial. In our review of the literature, we located 133 publications of various types (papers, reports etc.) that examine some aspect of dogs in parks and open spaces (50 % focussed solely on dogs). There has been an exponential growth in the cumulative number of articles (R (2) = 0.96; 82 % published since 1997); almost all pertain to temperate latitudes (97 %) and most to the northern hemisphere (62 %). Most articles focus on impacts on wildlife (51 %), zoonotic diseases (17 %), and people's perceptions regarding dogs (12 %). Articles mostly describe problems associated with dogs, while reports of low compliance with dog regulations are common. We outline six major findings regarding dogs in parks: (1) there is a paucity of information on dogs in parks, particularly in relation to their interactions with wildlife and regarding their management; (2) published studies are mainly restricted to a handful of locations in developed countries; (3) sectors of societies hold different views over the desirability of dogs in parks; (4) the benefits and risks of dogs to humans and park values are poorly documented and known; (5) dogs represent a notable disease risk in some but not all countries; and (6) coastal parks are over-represented in the literature in terms of potential negative impacts. Park managers globally require better information to achieve conservation outcomes from dog management in parks.

Listen to Us: Perceptions of Animal Voice and Agency.

In many contexts, the interests of nonhuman animals (hereafter "animals") are often overlooked or considered to be a lower priority than those of humans. While strong arguments exist for taking animal moral claims seriously, these largely go unheard due to dominant anthropocentric attitudes and beliefs. This study aimed to explore how animal interests might be best represented in the human world. We conducted interviews to investigate people's perceptions of what it means to speak for other animals and who can reliably represent animal interests. Using Grounded Theory analytical methods, we identified one major theme: "Animal voice", and its subthemes: "Animals do/do not have a voice", "Human language constructs realities and paradigms", and "Let animals speak". Our findings illustrate how human language constructs contribute to shaping the realities of animals by contextually defining them as voiceless. This has serious implications for animals, society, and the environment. Drawing parallels with the relevant literature, our results reflect calls for the social and political recognition of animal voice as fundamental to animal representation. We recommend future research to focus on developing ethical and compassionate approaches to understanding animal subjective experiences to empower and amplify animal voices.

Wildlife Photos on Social Media: A Quantitative Content Analysis of Conservation Organisations' Instagram Images.

Wildlife populations are vanishing at alarmingly high rates. This issue is being addressed by organisations around the world and when utilizing social media sites like Instagram, images are potentially more powerful than words at conveying crucial conservation messages and garnering public support. However, different elements of these images have been shown to potentially have either positive or negative effects on viewers' attitudes and behaviours towards wildlife and towards the organisation posting the image. This study used a quantitative content analysis to assess the most common and engaging elements of wildlife images posted to Instagram in 2020 and 2021, using Australian conservation organisations as a case study. A total of 670 wildlife images from the Instagram accounts of 160 conservation organisation Instagram accounts were coded and analysed. Results highlight that the most common image elements used included natural backgrounds, mammals and birds, and no human presence. In addition, it was found that the taxon of the animal featured in a post and the presence of humans did not impact engagement levels. Our findings highlight the potential for Instagram posts to feature and promote a wide range of currently underrepresented species, and for conservation organisations to be able to confidently share and post images that promote positive perceptions of both the animal and the conservation organisation.

Climate change adaptation for managing non-timber forest products in the Nepalese Himalaya.

Non-timber forest products (NTFPs) contribute to the well-being of mountain communities in many ways but their availability is being impacted by climate change. Policy and programs to enhance adaptation can alleviate these impacts, but to be effective they require an understanding of mountain community perceptions of climate change impacts on NTFPs and the perceived barriers to climate change adaptation. Here, we explored mountain communities' adaptation responses to the perceived impacts of climate change on NTFPs and people's barriers to adaptation using a structured questionnaire delivered as a field-based survey of 278 forest-dependent households from the Upper Madi Watershed of Nepal. We present a quantitative graphical exploration of the results to provide a simple overview of climate change impacts of NTFPs and local adaptation. The most common adaptation practices adopted by the mountain communities in the study area include the self-regulation of over-collection of NTFPs, alternative income generating activities, improved stoves, agroforestry, and sourcing alternative tools and materials. However, adaptation options to deal with increased invasive plant species and pest insect outbreaks appear to be challenging despite having the highest perceived impact on NTFPs. Adaptation practices are constrained by several factors such as the lack of predictability of extreme events and climate-related hazards, lack of technical knowledge, fatalistic beliefs and perceived lack of agency, and limited government support. Our results can inform policies and programs required for addressing the impacts of climate change on mountain communities in Nepal and other developing nations.

Survey data on climate change adaptation and barriers to adoption among smallholder farmers in Nepal.

The dataset presents the raw data collected through household surveys of smallholder farmers on adaptation to climatic variabilities and change in Sudurpaschim Pradesh (Far Western Province), Nepal. The dataset comprises farmers' responses on the likely determinants of adaptation decisions, actual uptake of adaptation measures, and the barriers to adaptation. We collected the data by conducting face-to-face interviews of 327 farmers using structured questionnaires in all nine districts representing the Mountain, Hill, and Terai agroecosystems in the province. We employed a stratified random sampling technique to recruit participants and interviewed them during December 2019 and March 2020. The interview methodology was approved by the Human Ethics Review Committee at Deakin University, Australia. The dataset is important for understanding the drivers of climate change adaptation and the barriers to adaptation to enhance the resilience of smallholder agriculture in far-western Nepal and can inform climate change adaptation strategies for the region and for the smallholder agroecosystems more broadly. The data are provided with this article.

Human Positioning in Close-Encounter Photographs and the Effect on Public Perceptions of Zoo Animals.

With the rising popularity of social media, conservation organisations and zoos need to understand its impact on public perceptions of the animals they house and their role in conservation. In addition, many zoos offer close-encounter experiences, and visitors frequently share images from these experiences online. This study measured the effects that viewing such encounter images had on public perceptions of both the zoo and the animals they saw. One of sixteen images was randomly presented to participants in two samples: one of Zoo Community followers and members of Zoos Victoria (n = 963), and a representative sample of the Australian public (n = 1619). Each image featured one of four animals (Eclectus parrot, Kangaroo Island kangaroo, Monteith's leaf insect, Centralian carpet python) and one of four human positions (human and animal touching, human and animal ~30 cm apart, human and animal ~1 m apart, animal alone). Results indicated that viewing different animals and the different human positions within these human-animal encounter images can affect public perceptions of zoo animals. In particular, the closer the proximity of a human to an animal in an image, the more likely respondents were to think that the animal was not displaying a natural behaviour and the more likely it was for General Public respondents to think that the animal would make a good pet. These findings can be used by zoos, wildlife tourism, and media organisations to ensure that they are sending clear, positive, and intended messages about zoo facilities and animals, as well as providing insights into animal encounter images in wider settings.

Resilience of smallholder cropping to climatic variability.

Smallholder agriculture is a major contributor to global food production and is vital for ensuring food security in many developing countries. Smallholder agriculture is a typically complex and heterogeneous social-ecological system that is especially susceptible to climatic variability. Research has often focused on examining climate impacts on crops in smallholder agriculture. However, the resilience of smallholder agriculture in terms of maintaining yield remains largely unexplored. We empirically quantified the resilience of rice, wheat and maize to climatic variabilities for the Far Western Province of Nepal. We calculated resilience indices (RI) comparing the anomalies of actual yield in agricultural statistics to the expected yields generated by a process-based yield simulation model for nine districts across the Terai, Hill and Mountain regions of the province. Based on the sustainable livelihoods framework, we then assessed the degree to which indicator variables correlate with resilience. The results demonstrate the variability of resilience across regions and crops. Terai, Hill and Mountain regions were found to be resilient in wheat, rice and wheat, and maize, respectively. Each region has maintained resilience in at least one crop suggesting that smallholder farmers have prioritised food subsistence. While Nepal's current Agricultural Development Strategy is focused on boosting yields in the Terai, we found the region to be less resilient overall compared to the Hill and the Mountain regions. Theory-driven capital indicators exhibited a weak and often contradictory relationship with resilience. Such indicators, used in isolation, could therefore misguide the resilience assessment in the absence of complementary fine-scale exploratory social research necessary to explain the drivers of resilience in smallholder agriculture and infer policy decisions.

Dataset of non-timber forest products use and impacts of recent climate change in the Upper Madi Watershed, Nepal.

This dataset presents data collected from household surveys from Upper Madi Watershed of Nepal describing the benefits of non-timber forest products (NTFPs) to people of mountain ecosystems, their perceptions of climate change, and perceived impacts of climate change on NTFPs ecosystem services. The data were collected from 278 households that were randomly selected from the four villages in the watershed during the period September to December 2019. The survey assessed socio-demographic information; collected and utilized NTFPs; perceptions of climate change, and; perceived impacts of climate change on NTFPs ecosystem services. These data are important in understanding the benefits of non-timber forest products in mountain ecosystems and the impacts of climate change as the benefits and impacts are currently not well understood. The data will be helpful in formulation and implementation of adaptation strategies to sustain the supply, protection, and management of NTFPs in mountain ecosystems.

A global paucity of wild bird feeding policy.

Feeding wild birds is a common and popular human-wildlife interaction throughout the world. However, controversy exists over the benefits and costs to the birds and humans involved. This bibliometric study documents wild bird feeding policies around the globe and examines environmental and socio-cultural factors which may influence the position adopted by these policies. We located 412 policies from 237 different countries, territories or dependencies (jurisdictions); 58.7% of jurisdictions had no information available online regarding wild bird feeding policy. Generalized Linear Modelling revealed that colder minimum ambient temperatures were associated with more pro-feeding policy settings. An index of controversy was calculated for each jurisdiction; most (77.6%) had no controversy. This study highlights the significant variation in policy settings for wild bird feeding across jurisdictions and controversy in almost a quarter of jurisdictions. Recommendations are made for the development of clear, consistent and readily available policy advice on this globally popular human-wildlife interaction.

Avian Assemblages at Bird Baths: A Comparison of Urban and Rural Bird Baths in Australia.

Private gardens provide habitat and resources for many birds living in human-dominated landscapes. While wild bird feeding is recognised as one of the most popular forms of human-wildlife interaction, almost nothing is known about the use of bird baths. This citizen science initiative explores avian assemblages at bird baths in private gardens in south-eastern Australia and how this differs with respect to levels of urbanisation and bioregion. Overall, 992 citizen scientists collected data over two, four-week survey periods during winter 2014 and summer 2015 (43% participated in both years). Avian assemblages at urban and rural bird baths differed between bioregions with aggressive nectar-eating species influenced the avian assemblages visiting urban bird baths in South Eastern Queensland, NSW North Coast and Sydney Basin while introduced birds contributed to differences in South Western Slopes, Southern Volcanic Plains and Victorian Midlands. Small honeyeaters and other small native birds occurred less often at urban bird baths compared to rural bird baths. Our results suggest that differences between urban versus rural areas, as well as bioregion, significantly influence the composition of avian assemblages visiting bird baths in private gardens. We also demonstrate that citizen science monitoring of fixed survey sites such as bird baths is a useful tool in understanding large-scale patterns in avian assemblages which requires a vast amount of data to be collected across broad areas.