RESUMEN
This paper presents a new method for combined measurements of persistent luminescence (PersL), thermoluminescence (TL), and mechanoluminescence (ML) of luminescent materials in the micrometer scale. Both the hardware and software designs have been illustrated in detail, and the experimental procedures to execute the emission map, PersL, TL, and ML measurements have been demonstrated. The PersL, TL, and ML properties of the SrAl2O4:Eu2+, Dy3+ micropowder, as well as the corresponding temperature variable emission spectra, have been measured. The results show good agreement with published investigations, indicating the accomplishment of designed functions. The instrument would be a powerful tool for exploring phosphorescent materials in the micrometer and smaller scales.
RESUMEN
WD repeat domain 5 (WDR5) serves an important role in various biological functions through the epigenetic regulation of gene transcription. Aberrant expression of WDR5 has been observed in various types of human cancer, including prostate cancer, breast cancer and leukemia. However, the role of WDR5 expression and its clinical implications in hepatocellular carcinoma (HCC) remain largely unknown. The present study investigated the WDR5 expression pattern in HCC. It was demonstrated that the mRNA and protein levels of WDR5 were upregulated in HCC cancer tissues compared with normal adjacent tissues using reverse transcription-quantitative polymerase chain reaction and western blotting. Furthermore, the elevated WDR5 protein level was significantly associated with the histological grade (P=0.038), tumor size (P=0.023), tumor-node-metastasis stage (P=0.035) and reduced long-term survival time. Additionally, it was demonstrated through the shRNA-mediated knockdown of WDR5 in HCC cells in vitro that WDR5 expression promotes cell proliferation using an MTT assay. Taken together, the results suggested that WDR5 overexpression may have an oncogenic effect in HCC, and may be a promising biomarker for the diagnosis and prognosis of HCC.