RESUMEN
Visible region active photocatalytic coatings are of interest for antimicrobial activity in low light applications or those employing LED lights with limited UV content. This work examined Ag and Fe doped titania nanoparticles (nTiO2) with varying dopant ranges in polyaspartic polymer coatings for potential light and dark activity. First, the Ag and Fe doped nTiO2 were synthesized by sol-gel chemistry with varying dopant concentrations, then characterized with respect to their size and aggregate size distribution, crystallinity, and surface and band gap features. The photocatalytic activity was then tested with methylene blue under both AM 1.5 G and visible light. From both sample sets (Ag and Fe doped nTiO2), the best photo catalytically active sample materials were chosen for antibacterial tests with gram-negative Escherichia coli (E. coli) and gram-positive Bacillus subtilis (B. subtilis) in (a) solution and (b) polyaspartic nanocomposites under UV and visible irradiation. The results showed that Ag doped nTiO2 samples delivered the best and excellent antibacterial action, even in the dark, attributed to both an enhanced band gap and surface area, as well as a combination of photocatalytic activity and Ag being present at the nanoparticle's surface. No leaching of Ag at room temperature was observed from the nTiO2 structure, giving potential for next generation coatings that are both light and dark active.
Asunto(s)
Antibacterianos/química , Hierro/química , Nanopartículas/química , Plata/química , Titanio/química , Antibacterianos/farmacología , Bacillus subtilis/efectos de los fármacos , Bacillus subtilis/efectos de la radiación , Catálisis , Escherichia coli/efectos de los fármacos , Escherichia coli/efectos de la radiación , Infecciones por Escherichia coli/tratamiento farmacológico , Humanos , Hierro/farmacología , Luz , Nanopartículas/ultraestructura , Plata/farmacología , Titanio/farmacología , Rayos UltravioletaRESUMEN
The dispersion of light-absorbing inorganic nanomaterials in transparent plastics such as poly(ethylene-co-vinyl acetate) (PEVA) is of enormous current interest in emerging solar materials, including photovoltaic (PV) modules and commercial greenhouse films. Nanocrystalline semiconductor or quantum dots (QDs) have the potential to absorb UV light and selectively emit visible light, which can control plant growth in greenhouses or enhance PV panel efficiencies. This work provides a new and simple approach for loading mesoporous silica-encapsulated QDs into PEVA. Highly luminescent CdS and CdS-ZnS core-shell QDs with 5 nm size were synthesized using a modified facile approach based on pyrolysis of the single-molecule precursors and capping the CdS QDs with a thin layer of ZnS. To make both the bare and core-shell structure QDs more resistant against photochemical reactions, a mesoporous silica layer was grown on the QDs through a reverse microemulsion technique based on hydrophobic interactions. By careful experimental tuning, this encapsulation technique enhanced the quantum yield (â¼65%) and photostability compared to the bare QDs. Both the encapsulated bare and core-shell QDs were then melt-mixed with EVA pellets using a mini twin-screw extruder and pressed into thin films with controlled thickness. The results demonstrated for the first time that mesoporous silica not only enhanced the quantum yield and photostability of the QDs but also improved the compatibility and dispersibility of QDs throughout the PEVA films. The novel light selective films show high visible light transmission (â¼90%) and decreased UV transmission (â¼75%).
RESUMEN
Ultraviolet (UV) radiation is known to cause an imbalance of the endogenous antioxidant system leading to an increase in skin cancer. Panax quinquefolium (American ginseng) polysaccharides (GPS) can inhibit such an imbalance due to its anti-oxidative and anti-inflammatory properties. The aim of this study was to investigate the therapeutic effects of topical formulations containing GPS nanoparticles (NPs) to inhibit UVB induced oxidative damage and skin cancer. Photoaging was conducted under UVB irradiation with a dose of 300 mJ/cm2 on SKH1 hairless mice. The treatment groups (n = 5) were as follows: sham control, native GPS, GPS NPs and fluorescent labeled GPS NPs. To compare the photoprotective performance, the topical formulations were applied before and after UVB induction (pre-treatment and post-treatment), followed by sacrificing the animals. Then, skin and blood samples were collected, and inflammatory cytokines production was measured using ELISA. Compared to the sham control, GPS NPs pre-treated mice skin and blood samples exhibited a significant lowering in all cytokine production. In addition, skin histology analysis showed that pre-treatment of GPS NPs prevented epidermal damage and proliferation. The results support that topical formulation containing GPS NPs can inhibit UVB induced oxidative damage and skin cancer.
Asunto(s)
Nanomedicina , Nanopartículas/administración & dosificación , Nanopartículas/uso terapéutico , Panax/química , Polisacáridos/uso terapéutico , Neoplasias Cutáneas/tratamiento farmacológico , Rayos Ultravioleta , Administración Cutánea , Animales , Citocinas/metabolismo , Daño del ADN , Femenino , Ratones Pelados , Nanopartículas/ultraestructura , Tamaño de la Partícula , Polisacáridos/administración & dosificación , Piel/patologíaRESUMEN
Plant polysaccharides (PS) such as American ginseng polysaccharide (GPS) have drawn immense interest in the field of immunoengineering, as they offer a way to actively control immune cell behavior and stimulation. These pharmacological activities have been limited by PS's inherent physicochemical properties including large molecular size, heterogeneity, and poor solubility. In this work, we hypothesized that by nanosizing and encapsulating GPSs, we could enhance their immunomodulation by increased penetration and absorption through the GI tract. Herein, GPS nanoparticles (NPs) of average size 20 nm (± 4 nm) were prepared using a microfluidic approach, then encapsulated within porous nanospheres (diameter 180 ± 10 nm) of biodegradable gelatin to enhance their oral delivery. To locate the GPS NPs inside the gelatin, we encapsulated fluorescent-labeled GPS in gelatin and analyzed using confocal microscopy. An in vitro investigation on tumor induced macrophage cell lines showed a concentration dependent enhanced immunostimulation with the encapsulated GPS NPs. The immunomodulation was then studied for different formulations of GPS through oral gavage in Swiss albino mice. The results showed that the production of proinflammatory mediators in blood samples was significantly increased for the encapsulated GPS in a dose- and time-dependent manner compared to other GPS treatments. This study shows that GPS and potentially other PS systems' immunomodulation properties can be significantly enhanced for use in simple oral drug delivery.
RESUMEN
Polysaccharides are a major active component of American ginseng root showing various biological activities including anti-carcinogenic, anti-aging, immunostimulatory and antioxidant effects. Although their biological activity has been reported by several groups, no research has explored their cellular uptake and biodistribution, owing to the lack of suitable detection techniques in living cells. This work examines a novel, simple and efficient fluorescent labeling procedure of ginseng polysaccharides (PS), in order to examine their cellular distribution using confocal microscopy. This procedure utilized a one-pot strategy with fluorescein-5-thiosemicarbazide (FTSC) to introduce a thiosemicarbazide group onto the aldehyde group at the reducing saccharide end to form a stable amino derivative through reductive amination. This polysaccharide-FTSC derivative was then characterized by GPC, UV, FTIR, photoluminescence and fluorescence microscopy to confirm attachment and any structural changes. The results demonstrated that the labeled ginseng PS nanostructure showed high fluorescence with minimal changes in PS molecular weight. The labeled PS exhibited almost no cytotoxicity effect against tumor induced macrophage cell lines (RAW 264.7) while retaining high immunostimulating activity similar to the non-labeled ginseng PS. Therefore, the developed approach provides a convenient and highly efficient fluorescent labeling procedure for understanding the mechanism of ginseng PS uptake in macrophage cell lines.
Asunto(s)
Factores Inmunológicos/química , Factores Inmunológicos/farmacología , Nanopartículas/química , Panax/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Polisacáridos/química , Polisacáridos/farmacología , Animales , Supervivencia Celular/efectos de los fármacos , Citocinas/metabolismo , Colorantes Fluorescentes , Macrófagos/efectos de los fármacos , Macrófagos/inmunología , Macrófagos/metabolismo , Ratones , Imagen Molecular , Nanopartículas/ultraestructura , Óxido Nítrico/metabolismo , Células RAW 264.7 , Espectroscopía Infrarroja por Transformada de Fourier , Coloración y EtiquetadoRESUMEN
North American (NA) ginseng root (Panax quinquefolium) has become of increasing scientific interest because of its immune-enhancing properties. Herein, we have developed a novel approach to synthesize ginseng polysaccharide nanoparticles (NPs) from NA ginseng for enhancing their immunostimulation. Nanoparticles of ginseng polysaccharide were prepared using a microfluidic device and compared to other conventional wet chemical processes including nanoprecipitation and reverse microemulsion. The morphology and size of the NPs were characterized by SEM, TEM, DLS and FTIR. Depending on the experimental conditions, microfluidics was found to provide unimodal polysaccharide spheres down to 20 nm (±4 nm) with very narrow particle size distributions. In addition, the immunostimulating effect of the polysaccharide NPs was investigated on Murine macrophage cell lines, with the results revealing an enhanced production of all proinflammatory mediators in a concentration dependent manner. The proposed microfluidic system has the advantages of ease of fabrication, simplicity, and a fast and low-cost process that is capable of producing ginseng polysaccharide NPs with demonstrated enhancement of immunostimulation of macrophage cell lines.