Bioinspired Cobalt Oxide Nanoball Synthesis, Characterization, and Their Potential as Metal Stress Absorbants.

Massive accumulation of heavy metals in agricultural land as a result of enhanced levels of toxicity in the soil is an emerging global concern. Among various metals, zinc contamination has severe effects on plant and human health through the food chain. To remove such toxicity, a nanotechnological neutralizer, cobalt oxide nanoballs (Co3O4 Nbs) were synthesized by using the extract of Cordia myxa. The Co3O4 Nbs were well characterized via UV-vis spectrophotometry, scanning electron microscopy, and X-ray diffraction techniques. Green-synthesized Co3O4 Nbs were exposed over Acacia jacquemontii and Acacia nilotica at different concentrations (25, 50, 75, and 100 ppm). Highly significant results were observed for plant growth by the application of Co3O4 Nbs at 100 ppm, thereby increasing the root length (35%), shoot length (48%), fresh weight (44%), and dry weight (40%) of the Acacia species with respect to the control. Furthermore, physiological parameters including chlorophyll contents, relative water contents, and osmolyte contents like proline and sugar showed a prominent increase. The antioxidant activity and atomic absorption supported and justified the positive response to using Co3O4 Nbs that mitigated the heavy-metal zinc stress by improving the plant growth. Hence, the biocompatible Co3O4 Nbs counteract the zinc toxicity for governing and maintaining plant growth. Such nanotechnological tools can therefore step up the cropping system and overcome toxicity to meet the productivity demand along with the development of agricultural management strategies.

Quantifying Serum Derived Differential Expressed and Low Molecular Weight Protein in Breast Cancer Patients.

BACKGROUND: Searching the biomarker from complex heterogeneous material for early detection of disease is a challenging task in the field of biomedical sciences. OBJECTIVE: The study has been arranged to explore the proteomics serum derived profiling of the differential expressed and low molecular weight protein in breast cancer patient. METHODS: Quantitative proteome was analyzed using the Nano LC/Mass and Bioinformatics tool. RESULTS: This quantification yields 239 total protein constituting 29% of differentially expressed protein, with 82% downregulated differential protein and 18% up-regulated differential protein. While 12% of total protein were found to be cancer inducing proteins. Gene Ontology (GO) described that the altered proteins with 0-60 kDa mass in nucleus, cytosol, ER, and mitochondria were abundant that chiefly controlled the RNA, DNA, ATP, Ca ion and receptor bindings. CONCLUSION: The study demonstrate that the organelle specific, low molecular weighted proteins are significantly important biomarker. That act as strong agents in the prognosis and diagnosis of breast cancer at early stage.